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Citations for Lonza :
1651 - 1700 of 4746 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... All cell lines were mycoplasma-negative (Mycoalert, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with smooth muscle cell bullet kit (Lonza) with 1% penicillin-streptomycin to expand cell numbers ...
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bioRxiv - Cancer Biology 2023Quote: ... mononuclear cells from healthy bone marrow specimens (Lonza) or cord blood cells from human umbilical cord blood specimens (Life South ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human bronchial epithelial cells (Lonza, CC-2540S) were selected based on age ...
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bioRxiv - Immunology 2023Quote: ... and rested overnight in T cell medium (Lonza). GFP+mCherry+ T cells were sorted and incubated in R10 medium for at least 2 h at 37°C and 5% CO2 ...
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bioRxiv - Pathology 2023Quote: Human umbilical vein endothelial cells (HUVECs; Lonza, C2519A) were treated with recombinant human osteopontin (rhOPN ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with smooth muscle cell bullet kit (Lonza). Following isolation ...
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bioRxiv - Cell Biology 2023Quote: ... CD34+ cells were resuspended in Opti-MEM (Lonza) at a concentration of 3-5×106 cells in a total volume of 750 μl ...
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bioRxiv - Microbiology 2024Quote: ... cell pellet was resuspended in RPMI-1640 (Lonza) containing 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: Healthy human lung airway epithelial cells (SAECs, Lonza, #CC-2547 ...
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bioRxiv - Neuroscience 2024Quote: ... P3 primary cell nucleofector kit (Lonza, V4XP-3032) and 4D nucleofector (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... OS-17 cells electroporated with SE buffer (Lonza) and the CM-104 program had the highest percentage of GFP+ cells ...
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bioRxiv - Cell Biology 2024Quote: Human Umbilical Vein Endothelial Cells (HUVECs, Lonza Scientific) and Bovine Aortic Endothelial Cells (BAECs ...
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bioRxiv - Cell Biology 2024Quote: PtK1 cells were transfected by nucleofector technology (Lonza) with recombinant pIRES2-AcGFP1 vectors expressing Tm2 and Tm4 respectively.
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bioRxiv - Cancer Biology 2024Quote: ... All cell culture reagents were provided by Lonza.
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bioRxiv - Molecular Biology 2024Quote: HASM cells and media were purchased from Lonza and cultured according to the vendor’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The resulting single cell suspension in HBSS (Lonza) was filtered through 100 and 40 μm meshes (to remove clumps of cells and aggregated debris) ...
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bioRxiv - Immunology 2024Quote: Human umbilical vein endothelial cells (HUVECs, C2519A; Lonza) were cultured in T75 flasks (Nunc™ EasyFlask™ ...
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bioRxiv - Neuroscience 2024Quote: ... An amount of 25 μl of pmaxGFP plasmid from Lonza Amaxa cell Nucleofector Kit V (Lonza, #VCA-1003) at a concentration of ∼0.2 μg/μl was aliquoted into a 10 cm plate and irradiated with 600 J/m2 of UV-C light with the lid open ...
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bioRxiv - Molecular Biology 2019Quote: ... and resuspended in 500 µL of Zymolyase buffer (NZ buffer with 20T zymolyase) and 500 µL LMP 2% (SeaKem LE agarose, Lonza, ref# 50001). Homogenized pellets were poured into the plug molds (∼100 µl/plug ...
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bioRxiv - Systems Biology 2020Quote: ... The pellets were resuspended in 10 mL of complete mammary epithelial growth medium with 2% v/v FBS without GA-1000 (MEGM; Lonza CC-3150). Cells were incubated at 37 °C for 1 h ...
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bioRxiv - Biophysics 2021Quote: All experiments using HUVEC were performed with an hTERT-immortalized HUVEC line previously described (28) and cultured in EGM-2 media (Lonza, CC-3162). For transient DNA transfections ...
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bioRxiv - Systems Biology 2022Quote: ... Early passage HUVECs were cultured in EGM™2- Bulletkit™ medium with growth supplements CC-3156 & CC-4176 purchased from Lonza. At 80% confluency ...
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bioRxiv - Molecular Biology 2019Quote: ... and Ode macrophages (17) were cultured in RPMI 1640 medium supplemented with 2 mM of L-glutamine (Lonza, catalogue number 12-702F) and 10 mM Hepes (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... and CRX +/tdtomato were maintained in reduced growth factor Matrigel (50ug/cm^2) in mTeSR1 plus with 1x Pen/Strep Amphotericin B (Lonza, 17-745E).
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg of pX330 neomycin-resistant vector containing each gRNA and 10 mM ssODN were co-transfected into 2 × 106 ESCs using a Nucleofector (Lonza, program A023). Cells were plated onto two 10 cm dishes containing hygromycin-resistant DR4 MEFs ...
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bioRxiv - Biophysics 2019Quote: Zebrafish between 9 and 13 dpf were anesthetized with Tricaine-S (MS-222, Pentaire; .008% w/v, buffered to pH 7) and embedded in 2% low melting point agarose (Lonza SeaPlaque, #50100) with .004% w/v Tricaine ...
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bioRxiv - Synthetic Biology 2022Quote: ... were ligated to the target fragments and full-length ligation products were purified via gel electrophoresis (2% MetaPhor agarose, Lonza, Basel, Switzerland). Purity and concentration of extracted fragments were determined using capillary electrophoresis (Fragment Analyser ...
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bioRxiv - Microbiology 2022Quote: ... pre-coated cell culture 6-well plate with EBM-2 endothelial media supplemented with 10% FBS and a growth factor bullet kit (Lonza, Walkersville, USA) 3 mL/well ...
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bioRxiv - Cell Biology 2024Quote: ... coated tissue culture polystyrene (TCPS) and maintained at 37° Celsius and 5% CO2 in endothelial growth medium (EGM-2 with bullet kit; Lonza, CC-3162) supplemented with 1% penicillin/streptomycin (Corning ...
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bioRxiv - Immunology 2023Quote: ... 80 µl of supernatant was removed and replenished with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were regularly passaged 2-3 times a week and routinely tested for mycoplasma contamination using MycoAlert Plus mycoplasma Detection kit (Lonza, #LT07-710).
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... with SmGM until confluency and then in SmBM (SmGM without complement) + 2% (v/v) FBS and antibiotics from the Bulletkit for SmGM (CC-4149, Lonza, Basel, Switzerland). SEP was added once at the beginning of the experiment at 100 µg/mL ...
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bioRxiv - Genomics 2024Quote: ... we generated Unrelated Control 2 iPSC using purchased human dermal fibroblasts from a healthy unrelated male (CC-2511, Lot No. 0000293971, Lonza, Basel, Switzerland). We conducted fibroblast collection and culturing ...
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bioRxiv - Cancer Biology 2021Quote: Cells with or without treatment were plated as single cell suspension at a density of 2000-5000 cells/well depending on the cell line in 24-well ultra-low attachment plates in Mammary Epithelial Cell Growth Medium (MEBM) medium (Lonza, Germany) supplemented with 4 μg/ml insulin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... HUVEC (primary Human Umbilical Vein Endothelial Cells, CC2517, LOT 0000482213) were maintained in Endothelial Cell Growth Medium Lonza (Lonza, CC-3121) with supplements and growth factors (Lonza ...
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bioRxiv - Bioengineering 2021Quote: ... we nucleofected HEK293FT-RfxCas13d cells with 225 μmol of synthetic crRNAs in 20μl reaction of SF Cell Line Nucleofector Solution (Lonza V4XC-2032) using the Lonza Nucleofector 4D (program CM-130) ...
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bioRxiv - Cell Biology 2021Quote: ... Human primary hepatic stellate cells from donors 3 and 4 were purchased as isolated hepatic stellate cells from Lonza (cat# HUCLS). Donor information is listed below.
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bioRxiv - Microbiology 2021Quote: ... Vero cell cultures were seeded at 1-3 × 104 cells/cm2 in Dulbecco’s minimal essential medium (DMEM, LONZA, Alpharetta, GA, USA) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... CHO-S cells were resuspended at a density of 4 mio cells/mL in ProCHO 4 medium (Lonza, #LZ-BE12-029Q) supplemented with 8 mM ultraglutamine (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... DNA electroporation was conducted following the Amanxa® Cell line nucleofector protocol using the Cell Line Nucleofector® Kit R (Lonza). 2 μg plasmid DNA was used per reaction and electroporation was carried out using the I-013 program for high expression efficiency of HeLa cells ...
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bioRxiv - Cancer Biology 2021Quote: ... and immortalized human Brain Microvascular Endothelial cells (HBMEC) were seeded at 5000 cells/cm2 in EBM supplemented with 5% FBS and EGM supplements (all products, Lonza Bioscience) until reaching confluence 42,43 ...
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bioRxiv - Molecular Biology 2020Quote: ... PrEC cells (Prostate Epithelial Cells) were cultured in PrEBM basal medium supplemented with PrEGM SingleQuots™ Supplements and Growth Factors (Lonza) and passaged when 80-90% confluent ...
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bioRxiv - Immunology 2022Quote: ... were delivered into the CD4+ Human T cells by using Amaxa 4D-Nucleofector System and P2 Primary Cell 4D-Nucleofector® X Kit S (Lonza) according to manufacturer’s instructions using the pulse code EH100 ...
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bioRxiv - Developmental Biology 2019Quote: ... E14 cells (5 × 106) were transfected with 10 μg of the appropriate plasmid with the Mouse ES Cell Nucleofector™ Kit (Lonza) and allowed to grow for 48h after transfection ...
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bioRxiv - Immunology 2019Quote: ... Leishmania tarentolae cells were transfected via electroporation (Nucleofector™ 2b Device, AmaxaTM Human T Cell Nucleofector™ Kit, Lonza, Basel, Switzerland) with the respective expression cassette containing the selective antibiotic marker Nourseothricin (NTC ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to a density of 1×106 cells and electroporated with 5μg of plasmid DNA (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Cells from each cell line were cultured for an additional 48 hours until total RNA isolation (Maxwell RSC simplyRNA kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 hiPS cells were nucleofected by Amaxa nuclefector device using Human Stem Cell Nucleofector® Kit 1 (VPH-5012, Lonza) and program B-016 with 4 μg of MyoD plasmid and 1 μg of the dual helper plasmid ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcribed mRNAs encoding the TCR α- and β-chains were mixed at a 1:1 ratio and electroporated in activated T cells on a Lonza 4D-NucleofectorTM device using the P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza) according to the recommendations of the manufacturer ...