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Citations for Lonza :
1451 - 1500 of 4746 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Human visceral pre-adipocytes were purchased from Lonza (PT-5005) and cultured according to the manufacturer’s instructions in PGM-2TM Preadipocyte Growth Medium-2 BulletKitTM from Lonza (PT-8002). Once cells were expanded ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were infected with virus dilutions (from 10-1 to 10-9) prepared in DMEM supplemented with 2% FBS and 1%Penicillin/Streptomycin mixture (Lonza Biosciences). Infected cells were incubated at 37°C for 7 to 15 days ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 2% haematocrit in O+ erythrocytes and RPMI-1640 medium (Lonza, BE12-167F or Gibco, 31870-025) supplemented to give the following final concentrations ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza) ...
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bioRxiv - Microbiology 2020Quote: ... Infected CD4 T cells were washed with PBS and 3 million cells per condition were resuspended in 20uL buffer P2 (Lonza) with 4μM IDT electroporation enhancer ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Genomics 2020Quote: ... 3 μL RNP complex solution was mixed with the cells and the cells were nucleofected using program DJ-110 on a 4D-Nucleofector (Lonza) with 16-well strips ...
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bioRxiv - Immunology 2021Quote: ... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
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bioRxiv - Cancer Biology 2020Quote: ... HMLE RAS Twist and HMLE RAS Twist ID1 cells were described previously (Stankic et al., 2013) and grown in MEBM (Mammary Epithelial Cell Growth Medium) (Lonza), supplemented with BPE (Bovine Pituitary Extract ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected by combining 5×105 viable cells with 400 ng plasmid DNA and nucleofection solution electroporation cuvettes (Lonza). Electroporation was performed according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Single cell suspensions were prepared by mechanical dissociation and expelling the cells through 40 μ using ACK lysing buffer (Lonza). Cells were washed and stained with an antibody cocktail of CD150 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were washed once in PBS and then resuspended in 1 ml of ACK Red Blood Cell lysis buffer (Lonza). After washing with PBS ...
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bioRxiv - Microbiology 2021Quote: ... between 1 x 106 and 1 x 107 cells were resuspended in 18 μl of P3 primary cells solution (Lonza), 2 μl of the restricted cassette (2-4 μg total ...
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Patients-derived iPS cells were electroporated with plasmids and single stranded DNA donor oligos using Human Stem Cell NucleofectorTM Kit 1 (Lonza). After 36 hours ...
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bioRxiv - Cell Biology 2021Quote: ... The RNP complex and 1.5 μg of a linearized homology-directed repair template plasmid were transfected into 2×105 – 5×105 nocodazole-arrested mitotic HeLa A12 cells using a Nucleofector and the associated Cell Line Kit R (Lonza) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Specific cells used were human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Lonza; Cat# CC-2540, male); human bronchial epithelial cells from a male smoking donor undergoing bronchoscopy for a non-cancer indication ...
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bioRxiv - Microbiology 2021Quote: ... Raw264.7 Ifit2 CRISPR cells were generated by electroporating low passage Raw264.7 cells with Ifit2 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...
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bioRxiv - Molecular Biology 2020Quote: ... H69 cells at 10,000 cells per well were mixed with 0.3% low melting temperature agarose (NuSieve GTG) (Lonza, Walkersville, MD) in complete medium ...
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bioRxiv - Microbiology 2022Quote: ... Ribonucleoproteins were introduced into 1 × 106 sub-confluent BlaER1 cells using 4D-Nucleofector X Unit and SF Cell line Kit (Lonza), applying program DN-100 ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Biophysics 2022Quote: ... 4-6 µg of DNA was mixed with the resuspended cells and electroporated using the Amaxa cell nucleofector (Lonza Laboratories) program Q-001 ...
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bioRxiv - Cancer Biology 2022Quote: ... and TILs were resuspended at a cell density of 1 million cells in 20 µL P3 electroporation buffer with supplement (Lonza) and then combined with 3 µL of RNP mixture and nucleofected using program DJ-110 (melanoma ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were introduced into HCT116 and MDCK cells using nucleofection with the 384-well HT Nucleofector and the SE cell line kit (Lonza) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Rinsed cells were resuspended in buffer P4 (mouse) or P2 (human) at 1-10 x 106 cells/20 μL (Lonza). Non-targeting Alt-R crRNA #1 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed once in PBS and then resuspended in 1 ml of ACK Red Blood Cell lysis buffer (Lonza). After washing with PBS ...
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bioRxiv - Cell Biology 2022Quote: HT29-N2 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) cell culture media (Lonza. Cat. Nº: BE12-604F/U1) supplemented with 10% (vol / vol ...
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bioRxiv - Bioengineering 2019Quote: ... approximately 200,000 cells were resuspended in a mixture containing 20 µl of P3 Primary Cell Nucleofector™ solution (V4XP-3032, Lonza), 5 µl of RNP complex for each guide (both guides AC and AD ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed once in PBS and then resuspended in 1 ml of ACK Red Blood Cell lysis buffer (Lonza). After two rounds of washing with PBS ...
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bioRxiv - Synthetic Biology 2019Quote: U2OS cells stably expressing eGFP.PEST or MDA-MB-231 cells were transfected with Cas9 ribonucleoprotein (RNP) and ssODN using the SE Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of DN-100 (U2OS ...
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bioRxiv - Cell Biology 2021Quote: ... was generated by the introduction of a plasmid carrying the EGFP gene under the control of the CAG promoter to EStTA5-4 cells using the Amaxa Mouse ES cells Nucleofector Kit (VPH-1001, Lonza).
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bioRxiv - Cell Biology 2021Quote: ... the culture medium was removed and the cells were replenished with UltraCULTURE serum-free cell culture medium (Lonza, BE12-725F). Medium containing secreted lentivirus was collected every 24 hours for a total of 72 hours and stored at 4°C ...
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bioRxiv - Immunology 2020Quote: Targeted gene’s expression was knocked-down in up to 5 x 106 cells using the P3 Primary Cell 4D-Nucleofector X Kit L (V4XP-3024, Lonza) with 90 µl P3 Primary cell solution and 100 pmol of corresponding si_RNA (resuspended in 10 ul RNAse-free H2O) ...
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bioRxiv - Cancer Biology 2020Quote: ... DRGFP expressing cells were electroporated in reagent T for H520 and H2228 or reagent L (Amaxa Cell Line Nucleofector Kit L, Lonza) for Calu6 with 10ug I-SceI using program X-005 for Calu6 ...
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bioRxiv - Systems Biology 2020Quote: 5×105 HAP1 cells were transiently transfected with 300 ng of plasmid using the SE cell line 4D-Nucleofector kit S (Lonza), on a 4D nucleofection unit (Lonza ...
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bioRxiv - Microbiology 2021Quote: Vero E6 cells (C1008; green African monkey kidney cells) were obtained from Public Health England and cultured in DMEM medium (Lonza) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... Naïve T cells expressing Cas9 were washed once with 1 mL pre-warmed recovery medium (Mouse T Cell Nucleofector Medium, Lonza) before electroporation ...
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bioRxiv - Immunology 2020Quote: ... and 5×106 cells seeded in 100-cm2 cell culture dishes in 12 mL RPMI supplemented with 10% fetal calf serum (Lonza), 1X non-essential amino acids (NEEA ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 NALM6 target cells were transfected with 1 μg plasmid using the Nucleofector 4D (SF cell line Kit, program CV-104, Lonza) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... the T cell / RNP / HDRT mixture was transferred onto a 16-well electroporation strip (20 µl = 106 cells per well, Lonza) or an electroporation cartridge (100 µl = 5×106 cells ...
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bioRxiv - Biochemistry 2022Quote: ... washed once with phosphate-buffered saline (PBS) and resuspended at 107 cells/mL in P4 Primary Cell Nucleofector Solution (Lonza). 20 µL of resuspended cells (corresponding to ~ 200,000 cells ...
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bioRxiv - Biophysics 2022Quote: ... The transfected cells were then co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) and cultured until virus-mediated CPE was observed ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF10A cells were purchased from ATCC and cultured with MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ (Lonza/Clonetics) supplemented with not supplemented with 100 ng/ml cholera toxin (Millipore) ...
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bioRxiv - Synthetic Biology 2022Quote: ... T cells were resuspended in P3 buffer (0.75-1 x 106 cells per 18 μl P3) from the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). 10 μg/μl Alt-R S.p ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once in PBS and then resuspended in 1 ml of ACK Red Blood Cell lysis buffer (Lonza). After washing with PBS ...
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bioRxiv - Microbiology 2022Quote: ... transfection was performed with 3.5 μg of PF16eYFPNeo introduced into 108 cells using a Human T-cell kit and IIb Nucleofector set to program X-001 (Lonza). Other transfections described in this work used the same conditions except with Tb-BSF buffer (90 mM sodium phosphate ...
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bioRxiv - Cancer Biology 2022Quote: ... and irradiated allogeneic PBMC (50 Gy) pooled from three donors as feeder cells in T-cell medium RPMI 1640 plus (Lonza):AIM-V (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Beads were mixed at a 1:1 ratio with cells and cells were cultured at a density of 1 x 106 cells/mL in complete X-Vivo 15 culture media (Lonza, 5% fetal bovine serum ...
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bioRxiv - Molecular Biology 2024Quote: ... the cell suspension was passed through 35 µm Cell Strainer Snap Cap and treated with red blood lysis (ACK buffer, Lonza) for 2 min at room temp ...