Labshake search
Citations for Lonza :
1401 - 1450 of 1833 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Enriched naïve PbTII GFP+ cells were resuspended in 20μl of P3 Primary buffer (P3 Primary Cell 4D-Nucleofector X Kit, Lonza) and mixed with sgRNA/Cas9 RNP ...
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bioRxiv - Neuroscience 2023Quote: Electroporation with freshly assembled RNP complex was performed on hiPSCs using the Human Stem Cell Nucleofector Kit 1 (Lonza) in conjunction with the Amaxa Nucleofector 2b system (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: ... hTERT ipn02.3 2λ cells were transfected by electroporation using the Amaxa Basic Nucleofector Kit for Primary Mammalian Fibroblasts (Lonza) and the program U-023 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 x 105 cells were transfected with the pU6-Sp-pegRNA-HEK3-insCTT plasmid (1,000 ng) using the SF Cell Line 96-well Nucleofector Kit (Lonza). Program code was FF-120.
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bioRxiv - Bioengineering 2023Quote: ... Cells were pulsed with protocol EO115 using the P3 Primary Cell 4D-Nucleofector Kit and 4D-Nucleofector System (Lonza). Cells were recovered immediately with warm media for 6 hours before transduction with CAR ...
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bioRxiv - Bioengineering 2023Quote: ... and 40 pmol of single-stranded oligonucleotide donor DNA (ssODN) were nucleofected to 0.25 × 106 HEK293T cells using the SF Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of CM-130 ...
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bioRxiv - Cell Biology 2023Quote: ... and mN designates the 2’-O-methylribonucleotides) was transfected into HAP1 cells (1.0 × 106 cells) by Nucleofector Kit V (Lonza) with a Nucleofector device (Lonza ...
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bioRxiv - Immunology 2023Quote: ... Cas9-gRNA ribonucleoproteins were assembled as described previously53 and nucleofected into 5×106 monocytes in 100μL nucleofection buffer (Human Monocyte Nucleofection Kit, Lonza) using a Nucleofector 2b (Lonza ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were targeted by nucleofection using the SE Cell Line 4D-Nucleofector™ X Kit S (Lonza #V4XC-1032). Cells were allowed to recover from nucleofection before sib-selection-based subcloning to isolate pure clonal cultures ...
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bioRxiv - Neuroscience 2023Quote: ... All CRISPR reagents were electroporated into iPSCs using the P3 Primary Cell 4D Nucleofector™ X Kit S (Lonza), as previously described.(25 ...
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bioRxiv - Bioengineering 2023Quote: ... with two phosphothiorate linkages on each end were mixed with 82 µL Nucleofector Solution V and 18 µL of Supplement Solution 1 according to manufacturer’s recommendations for the Cell Line Nucleofector Kit V (Lonza). 5×105 U2OS.EGFP parent cells were resuspended in the above mixture and nucleofected with a Lonza Nucleofector 2b Device (Kit V ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were transfected into human U2OS cells (ATCC HTB-96) by Amaxa electroporation with Nucleofector™ Kit V (Lonza) and plated in 6 well plates containing glass slides.
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bioRxiv - Synthetic Biology 2024Quote: ... K562 cells were electroporated with pDNA using the SF cell line 96-well Nucleofector kit (Lonza, Catalog#V4SC-2960) or SF Cell Line 384-well Nucleofector Kit (Lonza ...
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bioRxiv - Neuroscience 2024Quote: Nucleofections were done on cortical neurons using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, LZ-V4XP-3024), on the day of preparation and dissociation (DIV 0) ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...
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bioRxiv - Cell Biology 2020Quote: ... RNP complex was added to ssDNA GATA4 V267M repair template (IDT) and delivered to wildtype iPSCs by electroporation with the Amaxa human stem cell nucleofector starter kit (Lonza). Following electroporation ...
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bioRxiv - Cell Biology 2020Quote: ... Modified MTEC culture media is comprised of small airway basal media (SABM) with selected components from SAGM bullet kit (Lonza) including Insulin ...
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bioRxiv - Cell Biology 2020Quote: U-2 OS cells stably expressing spdCas9-GFP were nuclofected with Amaxa® Cell Line Nucleofector® Kit V (Lonza). Cells were detached with 0.05% trypsin/EDTA and spun down by centrifugation at 1000rpm for 5 min ...
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bioRxiv - Immunology 2021Quote: ... WT RAW264.7 cells were subjected to two rounds of nucleofection with Cas9-RNPs using the SF Cell Line 4D-Nucleofector X Kit S (Lonza) and the 4D Nucleofector apparatus precisely as specified by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... Constructs containing validated gRNAs were electroporated together with a vector expressing puromycin into hESCs using the P3 Primary Cell 4D-Nucleofector X kit (Lonza) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the RNP complexes were added to 1×105 cells in 40 μl of P3 nucleofection buffer from the 4D-Nucleofector X kit (Lonza). Half of the mixture was loaded to each well of a 16-well nucleofection cassette and nucleofected using the using E0-100 program with the 4D-Nucleofector Lonza Amaxa ...
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bioRxiv - Microbiology 2021Quote: ... were electroporated with 20 ug of guide plasmid and 60 ug of linearized repair plasmid using an Amaxa 4D electroporator and P3 Primary cell 4D Nucleofector X Kit L (Lonza) using programme FP158 as described (Collins et al. ...
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bioRxiv - Immunology 2022Quote: ... a total of 1.6 x 108 cells were transfected with 2 μM of the corresponding ASO using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza) and the EO-115 program ...
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bioRxiv - Genomics 2020Quote: ... Transfection of these iPSCs with the plasmid and Super piggyBacTM transposase mRNA (Transposagen) was done using the Human Stem Cell Nucleofector Kit 1 (VAPH-5012) by Nucleofector 2b Device (AAB-1001, Lonza) according to the manual ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 90 μL of nucleofection solution (16.2 μL of Supplement solution mixed with 73.8 μL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza) was mixed thoroughly with the cell pellet ...
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bioRxiv - Immunology 2021Quote: Control or transfected Lymphocytes (with Flag-wt-TDP-43 or Flag-NLS-mut-TDP-43 plasmid (1 μg) using nucleofection kit (Lonza)) were placed on coverslips (2 x 106 cells in sterile glass coverslips-Ø 12 mm ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Physiology 2019Quote: ... 10% AIM-V and 100 units/ml penicillin/ streptomycin and transiently transfected using Nucleofector™ Kits for Human Melanocytes (Lonza) or magnetofection with PolyMag Neo magnetic beads (OZ Biosciences) ...
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bioRxiv - Genetics 2019Quote: ... Electroporation was performed using the Human Adult Dermal Fibroblast Nucleofector™ Kit and the Nucleofector™ II/2b device (Lonza). Both cell lines were harvested 72-hours post-transfection for qRT-PCR ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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bioRxiv - Bioengineering 2019Quote: ... and grown in bronchial epithelial growth medium (BEGM) supplemented with SingleQuot additives from Lonza (BEGM Bullet Kit, reference CC-3170) and 50 μg/mL G-418 at 37°C and 5% CO2.
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bioRxiv - Neuroscience 2019Quote: ... the neurons were resuspended in DMEM/F12 and electroporated using a P3 Primary Cell 4D-Nucleofector X Kit L (cat. no. V4XP-3012, Lonza) in accordance with the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Each chamber was connected to a reservoir that was pre-filled with 15 mL of warm serum-free EGM-2 (endothelial cell growth medium-2 without serum added from the kit, Lonza). ECFCs were harvested and resuspended in serum-free EGM-2 and added to the reservoirs to reach an overall cell density of 125,000 cells/mL ...
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bioRxiv - Genomics 2020Quote: ... 5 million cells were transfected with 5 μg of DNA FAIRE-STARR library plasmid using the Amaxa Nucleofector kit V (Lonza). For each condition ...
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bioRxiv - Systems Biology 2021Quote: ... and 2×106 iPSCs were mixed with pX459-sgRNA vector and ssDNA repair donor template and electroporated with Lonza 4D-Nucleofector system using the P3 Primary Cell Nucleofector Kit (Lonza). The iPSCs were then recovered in mTeSR1 medium for 48 hours before adding puromycin for 3-day selection ...
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... 200,000 cells were electroporated with 1000 ng adRNA plasmid or 48 pmol of IVT RNA using the Amaxa SF cell Line 4D-Nucleofector X kit (Lonza) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... The efficacy of siRNA targeting Kdm6a was assessed by electroporation of neurons using a 4D-Nucleofector X Unit and the corresponding P3 Primary Cell nucleofection kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in 37 °C and 5% CO2 incubators and routinely tested negative for mycoplasma contamination using the MycoAlert Kit (Lonza).
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bioRxiv - Cell Biology 2021Quote: ... Patients-derived iPS cells were electroporated with plasmids and single stranded DNA donor oligos using Human Stem Cell NucleofectorTM Kit 1 (Lonza). After 36 hours ...
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bioRxiv - Cell Biology 2021Quote: ... The RNP complex and 1.5 μg of a linearized homology-directed repair template plasmid were transfected into 2×105 – 5×105 nocodazole-arrested mitotic HeLa A12 cells using a Nucleofector and the associated Cell Line Kit R (Lonza) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: iPSNs were transfected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza). POM121 ...
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bioRxiv - Microbiology 2021Quote: ... Both resuspensions were mixed in a 100 μl cuvette (P3 Primary cells 4D-Nucleofector X kit L, V4XP-3024, Lonza). The programme FI-158 was used for electroporation ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Microbiology 2020Quote: ... All cells used in this study were cultured in a humidified incubator at 37°C with 5% CO2 and routinely tested and certified as mycoplasma-free using the MycoAlert kit (Lonza). STR analysis (Idexx ...
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bioRxiv - Immunology 2021Quote: ... MICB or ULBP-1 genes after optimizing nucleofection conditions using primary cell 4D nucleofector kit and 4D nucleofector system (Lonza). After 48 hours of culture ...
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bioRxiv - Cell Biology 2022Quote: ... were nucleofected with either PGK-mO-Dam or PGK-mO-Dam-Zfp266 plasmids using the P4 Primary Cell 4D-Nucleofector X Kit (Lonza). 5 replicates were performed in total ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 NSCs were mixed with 5 µg of corresponding DNA with the Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) and pulse was delivered using the CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5μg of selected plasmid (control, empty vector (EV) or containing guide RNAs using Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...