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Citations for Lonza :
1351 - 1400 of 1833 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The siRNA duplexes were used to transfect purified human naïve B cells using the Human B Cell NucleofectorTM Kit (VPA-1001, LONZA). Transfected B cells were then stimulated with CpG ODN 2395 plus IL-2 and IL-21 for 96 h before genomic DNA extraction for analysis of Sμ-σδand Sμ-Sγ1 DNA recombination ...
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bioRxiv - Neuroscience 2020Quote: ... 2×105 dissociated neurons were transfected with Lonza Nucleofector using the Basic Neuron SCN Nucleofector kit (Lonza, Basel, Switzerland). Transfected neurons were incubated for indicating days and processed for subsequent analyses.
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bioRxiv - Cancer Biology 2021Quote: ... The siRNA transfections for primary BMDMs and pancreatic fibroblasts were performed using the Mouse Macrophage Nucleofector™ Kit (Lonza) and Nucleofector™ 2b Device (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: ... E4ORF1-transduced primary human umbilical vein endothelial cells (HUVECs) were cultured using the EGM-2 Bullet Kit (Lonza, Germany). All cell lines were kept at 37°C and 5% CO2 in a fully humidified incubator and negatively tested for mycoplasma by PCR.
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bioRxiv - Systems Biology 2021Quote: ... Cells were transfected with 250 nM of siRNA and 1 μl of control pMax GFP (Nucleofector X kit, Lonza), using the CU-133 program ...
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bioRxiv - Microbiology 2021Quote: ... berghei ANKA using the parasite nucleofector II kit and the Nucleofector II device with the U-033 program (Lonza). Transfected parasites were injected intravenously into 5 - 7 weeks old female ddY mice ...
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bioRxiv - Microbiology 2021Quote: ... Relative adenylate kinase levels were measured on 40μL of supernatants via the ToxiLightTM Non-Destructive Cytotoxicity BioAssay Kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... pCAGIG-hA3A-BE3 plasmid was delivered into cells using SF Cell Line 4D-Nucleofector X Kit (Lonza, #V4XC-2032) using programme CA-137 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 x 106 NSCs were mixed with 2.5 µg of corresponding DNA using Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) with CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of DNA was used to transfect the cells using the Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 300 pmol of HDR oligonucleotide was electroporated into one million HEK293 Flp-In T-Rex cells along with 2.5 μg each of pSpCas9(BB)-2A-Puro and BiP gRNA plasmid (Amaxa kit R, program A-24; Lonza). Immediately after electroporation ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in 100 μL nucleofector solution at a concentration of 1.5 x 106 cells/100 μl following the instructions of the Nucleofector Kit V (Lonza). In vitro transcribed RNA (5 μg ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 million cells were transfected with 2μg of donor vector and 2μg of each AAVS1 ZFN expression plasmids using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza). Cells were seeded in E8 medium supplemented with 10µM ROCK Inhibitor Y-27632 (Selleckchem) ...
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bioRxiv - Developmental Biology 2020Quote: ... expressing Cas9 and single sgRNAs targeting upstream and downstream regions of the lncRNA promoter/locus were co-transfected into 1.5 × 106 H1 hESCs using the Human Stem Cell Nucleofector Kit 2 (Lonza) and the Amaxa Nucleofector II (Lonza) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were washed with RPMI before being re-suspended in Lonza electroporation solution from Kit C (Lonza, Basel, Switzerland) and electroporated using program W-001 using the Amaxa nucleofector (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... Pre-assembled Cas9-gRNA RNPs were electroporated into cells using Lonza Human T cell Nucleofector kit (Lonza, VPA-1002) and the Nucleofector2b equipment ...
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bioRxiv - Cell Biology 2022Quote: ... the genetic transformation of RAW 264.7 cells was obtained by nucleofection using the Amaxa Cell Line Nucleofector Kit V (Lonza) for RAW 264.7 and following the protocol for Amaxa Nucleofector ...
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bioRxiv - Immunology 2022Quote: ... were cultured in EBM-2 supplemented with EGM-2 endothelial growth SingleQuot kit supplement & growth factors (Lonza, the Netherlands). All of the cell lines used in this study tested negative for the presence of Mycoplasma spp ...
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bioRxiv - Immunology 2022Quote: ... In vitro activated or naïve P14 cells were resuspended in 20 μl of P3 (P3 Primary Cell 4D-Nucleofector X Kit; Lonza), mixed with sgRNA/Cas9 RNP and electroporated using a Lonza 4D-Nucleofector system (CM137 ...
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bioRxiv - Immunology 2022Quote: RAW 264.7 macrophage-like cells were transiently transfected by nucleofection using Amaxa cell line kit V (Lonza; VACA-1003) as previously described115 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected by nucleofection using the Lonza Cell Line Nucleofector® Kit V (Lonza, Basel, Switzerland, #VVCA-1003) and the Amaxa Nucleofector II device ...
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bioRxiv - Molecular Biology 2020Quote: ... 1×106 EBdCas9 cells were transfected with the above RNP complex and 100μM of ssDNA (HDR)(Sup Table 8) using AMAXA electroporation kit (LONZA) and plated in TeSR media (supplemented with 5μM ROCKi) ...
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bioRxiv - Synthetic Biology 2019Quote: INS-1E cells were transfected with Cas9 RNP and ssODN using the SF Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of DE-130 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1,000,000 cells were nucleofected with 3.33 µg of SQT1665 and 1.66 µg of plasmid expressing gRNA using SE Cell Line 4D-NucleofectorTM X Kit L (Lonza) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... 20 µg ssODNs and 4 µg pSpCas9(BB)-2A-GFP construct using Human Stem Cell Nucleofector Kit 2 (Lonza). For the generation of UE-RASGEF1A-int1-KO and PIK3C2B-int10-KO hPSC lines ...
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Developmental Biology 2020Quote: ... HLECs were grown on culture dishes or glass slide coated with 0.2% gelatin and were maintained in EGM-2 EC Growth Medium-2 Bullet Kit (Lonza). All experiments were conducted using cells until passage (P ...
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bioRxiv - Physiology 2021Quote: Bacterial endotoxin measurement in portal serum samples was performed using the Lonza Pyrogent turbidometric LAL assay kit (Lonza, N383) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were nucleofected with both RNPs using the 4D-Nucleofector and P3 Primary Cell 4D-Nucleofector X Kit (Lonza). After 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... washed twice with PBS and 0.25×106 cells per condition resuspended in 20μL supplemented cell line solution (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were then transferred to the RNP solution ...
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bioRxiv - Developmental Biology 2021Quote: ... HESCs were transfected with a total of 4ug DNA in a ratio 2:3 (gRNAs:donor template) using the Amaxa P3 Primary Cell 4D-Nucleofector Kit (Lonza). Puromycin was added 3 days after electroporation at a concentration of 0.5ug/ml ...
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bioRxiv - Immunology 2021Quote: Endothelial cells were transfected using the Nucleofector primary mammalian endothelial cell kit (Cat. #VAPI-1001, Lonza Biosciences, Durham, CA), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection using electroporation was performed according to the manufacturer’s protocol using a P3 primary cell 4D-Nucleofector kit (Lonza) and program 138 for human keratinocytes ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were immortalized with pBabe-SV40Tag by electroporation using the Amaxa Mouse/Rat Hepatocyte Nucleofector Kit (Lonza, VPL-1004). Immortalized MEF lines were genotyped ...
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bioRxiv - Genetics 2019Quote: ... Primary VSMCs were cultured on gelatinized dishes in SmBM medium supplemented with the SmGM-2 kit (CC-3182, Lonza). Unless otherwise specified ...
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bioRxiv - Developmental Biology 2022Quote: ... 1x106 A2lox mESCs (WT and Bra-/-) were transfected with 2.5 μg of linearized vector using the Nucleofector ESC kit (Lonza) and G418 selected (350 μg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... primary hepatocytes were seeded 400,000 cells per well in complete hepatocyte plating media (HCM kit, Lonza, Cat# CC-3198). After four hours of incubation at 37 °C and 5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were mixed with complete p3 electroporation solution (from a Lonza 4D nucleofection kit; Lonza Group AG, Basel, Switzerland) followed by addition of the RNP complex and 500 pmoles of donor ssODNA (Alt-R HDR ...
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bioRxiv - Cell Biology 2022Quote: A sub-cloned population of BEL-A cells (1.0 × 105) were resuspended in CD34+ nucleofection kit buffer (Lonza Biosciences) containing 18 pmol Cas9 ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The resuspended cells were immediately transferred into the cuvette of the P3 Primary Cell Nucleofector Kit (Lonza; V4SP-3096) and electroporated using code EH-100 on the Lonza 4D-Nucleofector ...
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bioRxiv - Systems Biology 2022Quote: ... Electroporation mix was performed using the Lonza Amaxa SE Nucleofection 4D kit in the 100μl format with the 4D-NucleofectorTM X Unit (Lonza) machine ...
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bioRxiv - Neuroscience 2022Quote: Nucleofections were done on cortical neurons using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, LZ-V4XP-3024), on the day of preparation and dissociation (DIV 0) ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells were transfected using the Amaxa® Cell Line Nucleofector® Kit L (Lonza, Basel, Switzerland), program C-05 as previously described(46) ...
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bioRxiv - Bioengineering 2024Quote: ... HeLa cells (2×105 cells/condition) were electroporated using the SE Cell Line 96-well Nucleofector™ Kit (Lonza) and the EH-100 pulse code (Nucleofector 4D ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X S kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 400 pmol of siRNA duplex was introduced into 3.0 × 106 cells using the Cell Line 96-well Nucleofector Kit SF (Lonza) with program DG150 of the 96-well Shuttle Device (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... we modified the protocol and compared site-by-site the transfection efficiency of the Basic Parasite Nucleofector Kit (Lonza) and Tb-BSF protocol (Schumann Burkard et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 cells were transfected with the PB-GFP-P2A-ΔNβCAT plasmid and a Piggybac transposase-coding plasmid using the P3 primary cell 4D nucleofector kit (Lonza). After 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and sgRNAs against LRRK2 (crRNAs ordered from IDT) were introduced into iPSCs via nucleofection (Lonza P3 kit # V4XP-3032) as described before(75) ...
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bioRxiv - Molecular Biology 2022Quote: ... 8×107 promastigotes were transfected with 1 µg (≈ 1,68.1011 molecules) of the linearized barcoding library using the Basic Parasite Nucleofector™ kit 1 (Lonza) with the U-033 program ...