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Citations for Lonza :
1201 - 1250 of 4746 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... one plate was prepared with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were authenticated by Brca1/2-specific PCR-based genotyping (mouse)7,8 and they were regularly tested for mycoplasma contamination (Mycoalert, Lonza).
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bioRxiv - Bioengineering 2023Quote: ... cAP-0001GFP) were cultured in 0.2% gelatin-coated T75 tissue culture flasks with EGM-2 medium (Lonza, CC-3162). The medium was changed every other day ...
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bioRxiv - Neuroscience 2024Quote: ... dissociated neurons were electroporated with cDNA (2 µg) or siRNA (Table S2) using an Amaxa nucleofector (program-005, Lonza) and mouse neuron nucleofectorTM kit (VPG-1001 ...
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bioRxiv - Immunology 2019Quote: ... Hybridoma cells were electroporated using the SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2024) with the program CQ-104 ...
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bioRxiv - Cell Biology 2019Quote: ... MCF10A (human breast epithelial cell line) were cultured in Mammary Epithelial Cell Growth Medium (Lonza, CC-3151) with supplements and growth factors (Lonza ...
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bioRxiv - Immunology 2021Quote: ... 1-10 million activated P14 cells were re-suspended in 20 μL primary cell nucleofection solution (Lonza), then mixed and incubated with the crRNA/Cas9 mix for 2 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... T cells were cultured in human T cell medium consisting of X-VIVO 15 (Lonza #04-418Q), 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165 ...
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bioRxiv - Biophysics 2021Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
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bioRxiv - Synthetic Biology 2021Quote: ... T cells were cultured in human T cell medium consisting of X-VIVO 15 (Lonza #04-418Q), 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165 ...
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bioRxiv - Bioengineering 2022Quote: ... Electroporations were performed in K562 cells using the SF Cell Line 4D-Nucleofector X Kit S (Lonza) per manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... 18-20μL of cells (4.5-20 *104 cells) were then plated into 16-well nucleocuvette strips (Lonza). Each well had also previously received 4μL of ribonucleoprotein ...
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bioRxiv - Immunology 2019Quote: ... 10×106 Jurkat T cells were electroporated using the P2 primary Cell 4D-nucleofector kit from Lonza with program EH-100 ...
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bioRxiv - Cell Biology 2019Quote: ... U2OS and RPE1 cells were transfected using Cell Line Nucleofector Kit V and a Nucleofector electroporator (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... U2OS.eGFP.PEST cells were transfected with the RNP complex using the SE Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of DN-130 ...
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bioRxiv - Immunology 2021Quote: ... Human Dermal Microvascular Endothelial Cells (HDMEC) and Human Lung Microvascular Endothelial Cells (HLMEC) were purchased from Lonza Bioscience (Houston ...
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bioRxiv - Immunology 2020Quote: ... All transfection experiments were performed with Amaxa Cell Line Nucleofector Kit V for monocytic cells(Lonza, Germany) by using Amaxa Electroporation System (Amaxa Inc ...
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bioRxiv - Cell Biology 2020Quote: ... After the cells reached 100% confluence the cells were exposed to adipogenic induction media (BulletKit, Adipogenesis, Lonza). The induction medium consisted of 1 mM dexamethasone ...
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bioRxiv - Cell Biology 2020Quote: ... After the cells reached 100% confluency the cells were exposed to osteogenic induction media (BulletKit, osteogenic, Lonza). The induction medium consisted of DMEM-LG ...
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bioRxiv - Microbiology 2021Quote: Madin-Darby canine kidney cells (MDCK cells; RRID: CVCL_0422) were maintained in minimum essential medium (MEM; Lonza) supplemented with 2 mM GlutaMAX (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza-BioResearch, #: V4XP-3032) and Amaxa 4D- Nucleofector (Lonza-BioResearch) ...
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bioRxiv - Immunology 2022Quote: ... approximately 5 million cells were suspended in 74 μl of P4 Primary Cell solution (Lonza, VSOP-4096). In parallel ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured and expanded in complete SAGM (small airway epithelial cell growth medium; Lonza, CC-3118) containing TGF-β/BMP4/WNT antagonist cocktails and 5 μM ROCK inhibitor Y-27632 (Selleckbio ...
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bioRxiv - Microbiology 2022Quote: Human umbilical vein endothelial cells (HUVECs) and Human Dermal Lymphatic Endothelial Cells (LEC) were obtained from Lonza and PromoCell ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell-conditioned media (CCM) was obtained from either U2-OS cells grown under serum-free (MEGM, Lonza) or serum-containing conditions (10% FBS) ...
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bioRxiv - Genomics 2022Quote: ... One million sorted Pro-E cells were resuspended with 100 μl solution P3 for primary cells (Lonza); preassembled RNP were then added to the cell suspension for nucleofection ...
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bioRxiv - Bioengineering 2024Quote: ... the cell culture medium was aspirated and cells were washed with phosphate-buffered saline (PBS; Lonza, Switzerland) followed by cultured in an exosome-production medium for 48 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... T cells were cultured in human T cell medium consisting of X-VIVO 15 (Lonza #04-418Q), 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165 ...
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bioRxiv - Immunology 2024Quote: ... cells were washed once with PBS and resuspended in T cell nucleofection solution (Lonza, Cat: VPA-1002) containing plasmids of choice and nucleofected using the T-023 program on the Amaxa IIb nucleofector ...
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bioRxiv - Immunology 2024Quote: ... T cells were cultured in T cell medium containing X-VIVO™ 15 media (Lonza, Basel, Switzerland), 10% human AB serum (Corning Inc. ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were cultured in a T cell medium of X-VIVO-15 (Lonza, Basel, Switzerland) with 5% Human AB Serum (MilliporeSigma ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... MCF10A cells were obtained from ATCC (CRL-10317) and cultured in Mammary Epithelial Cell Growth Media (Lonza) supplemented with MEGM Mammary Epithelial Cell Growth Medium BulletKit (Lonza) ...
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bioRxiv - Bioengineering 2023Quote: Nucleofection in T cells were performed using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza) according to the manufacturer’s instruction with minor modifications ...
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bioRxiv - Microbiology 2023Quote: siRNAs targeting bovine hnRNPM were introduced into BL3.1 cells using Amaxa cell line Nucleofector kit V (Lonza) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: K562 cells stably expressing proinsulin [12] and HEK293T cells for virus production were cultured in IMDM (Lonza). All media were supplemented with 10% FCS ...
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bioRxiv - Bioengineering 2023Quote: ... 1×106 cells were nucleofected using the Lonza SF Cell Line 4D-Nucleofector Kit (Lonza V4XC-2012) and a Lonza 4D Nucleofector (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and these cells were transferred to a cuvette (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) for nucleofection in a 4D-Nucleofector (Lonza ...
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bioRxiv - Cell Biology 2023Quote: U2OS cells and the stable cell lines generated were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Lonza) cell culture media supplemented with 10% (vol/vol ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... cells were transfected using Nucleofection with an Amaxa SF Cell Line 4D-Nucleofector X kit S (Lonza) and program FF-113 (HT1080 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected by nucleofection using the Lonza Cell line Nucleofector Kit V (Lonza, Basel, Switzerland, #VVCA1003) and the Amaxa Nucleofector II device ...
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bioRxiv - Biophysics 2023Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
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bioRxiv - Neuroscience 2023Quote: ... Cell- plasmid solution was transferred to the electroporation cuvette (AmaxaTM P3 primary cell 4D- NucleofectorTM X, Lonza) and electroporated using the program CB-150 of the Nucleofector ...
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bioRxiv - Synthetic Biology 2021Quote: ... The product was purified and 2 µg were added to the RNP and diluted in 100 µL P3 nucleofection buffer (Lonza). This mixture was nucleofected with 2×106 stimulated human primary T cells using the 4D-Nucleofector (Lonza ...
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bioRxiv - Developmental Biology 2020Quote: ... The red blood cell lysis was performed by resuspending the pellet in 2 ml sterile ACK (Ammonium-Chloride-Potassium) lysis buffer (ACK Lysing Buffer, Lonza) and incubating on ice for 5 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... at passages 4-7 were grown to confluence on gelatin-coated plates and maintained using complete EGM-2 media (Lonza). HKi002 was maintained at room temperature for 30 min rotating before use ...
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bioRxiv - Biochemistry 2019Quote: hCMEC/D3 cells at passages 30–37 were grown to confluence on collagen-coated plates and cultured using in EGM-2 MV medium and supplemented with complements obtained from the manufacturer (Lonza) as previously reported (37) ...
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bioRxiv - Cell Biology 2019Quote: ... were isolated from neonatal foreskin and cultured on 0.1% gelatin-coated culture dishes in 0.1% gelatin-coated culture dishes in EGM-2 MV media (Lonza #cc-3202). Both HUVEC and MEC were used before passage five in all experiments and the endothelial identity of the cells was confirmed by immunofluorescence microscopy with antibodies to endothelial markers PECAM-1 and VE-cadherin.
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bioRxiv - Genomics 2021Quote: ... two million H9 hESCs were co-electroporated with the appropriate knockin vector (5 μg) and plasmids encoding AAVS1-targeting TALENs (2 μg; addgene, 59025 and 59026) using an Amaxa 4D Nucleofector system (Lonza). Serial cell dilutions were then seeded in six-well plates in E8 supplemented with Y-27632 (10 μM) ...
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bioRxiv - Developmental Biology 2022Quote: Primary HDLECs were isolated from neonatal human foreskins as described previously (74) and cultured from passages 5 – 7 on fibronectin-coated plates with EGM-2 MV growth media (Lonza) supplemented with human VEGF-C (R&D) ...
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bioRxiv - Developmental Biology 2021Quote: ... and E15.5 (n=2–6) Six2-TROMA-1 stained kidneys were embedded in 1 % low melting agar (50100; Lonza Group). The lateral cortex of the kidneys was imaged with a Nikon A1R MP+ multiphoton microscope (Tokyo ...