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Citations for Lonza :
51 - 100 of 2027 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... together with a plasmid encoding the piggyBac transposase in a 3:1 ratio (vector:transposase) using the P3 Primary cell 4D-Nucleofector™ kit (Lonza). Prior to targeting ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Measured tissues originated from six F508del homozygous patients (CF patient #1: CF-AB0609, female, 31 years-old; CF patient# 3: #28388-0000450918, Lonza, male ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were first electroporated with a PiggyBac-configured plasmid containing the Dox-inducible NbALFA-ABEL degrader cassette and a plasmid encoding the PiggyBac transposase at a 3:1 mass ratio via nucleofection (solution E with program CM138) according to manufacturer’s instructions (Lonza Inc.), followed by puromycin selection (5μg/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... The optimised sequences were used to design gBlocks with appropriate overhangs (Fig. 1-3) and cloned into pMAX-GFP plasmid from Lonza, digested with KpnI and SacI (Fig ...
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bioRxiv - Bioengineering 2024Quote: SP8 or DP T cells were isolated as described above and stimulated in vitro using irradiated K562 CD19-CD137L aAPCs at a 1:3 aAPC:T cell ratio in X-VIVO™ 15 (Lonza), supplemented with 5% human AB serum (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... HESCs were transfected with a total of 4ug DNA in a ratio 2:3 (gRNAs:donor template) using the Amaxa P3 Primary Cell 4D-Nucleofector Kit (Lonza). Puromycin was added 3 days after electroporation at a concentration of 0.5ug/ml ...
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bioRxiv - Cell Biology 2022Quote: Human umbilical vein endothelial cells (HUVEC) were cultured between passage 3 and 6 in EGM-2 complete medium (Lonza)3,52–55 ...
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bioRxiv - Cancer Biology 2024Quote: ... and (iv) the NDF-2 and NDF-3 cell lines (human neonatal dermal fibroblast cell lines, #CC-2509; Lonza Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... Vero cell cultures were seeded at 1-3 × 104 cells/cm2 in Dulbecco’s minimal essential medium (DMEM, LONZA, Alpharetta, GA, USA) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Immunology 2021Quote: ... 2.106 T cells were resuspended in 20 µl of nucleofection solution with 3 µl or 4 µl RNP and transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from healthy donors (n=3) as described[4] and seeded on 6 cm dishes within BEGM media (Lonza) before transduction with lentivirus (empty vector (Origene ...
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bioRxiv - Immunology 2021Quote: Calu-3 epithelial lung cancer cells were cultured in DMEM (Lonza) supplemented with 10% heat inactivated fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: ... 3 ml of lung digestion buffer containing HBSS (Lonza, BE10-508F), 5% FBS ...
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bioRxiv - Microbiology 2022Quote: ... a 3 ml of DMEM containing 0.5% SeaKem ME agarose (Lonza), 5% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were size-selected using 3% NuSieve agarose gels (Lonza) followed by gel extraction using QIAEX II reagents (Qiagen) ...
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Bioengineering 2023Quote: ... Adipose-derived stem cells (Passage 3-5) (Lonza, Walkersville, MD, USA) were cultured in a basal medium consisting of DMEM/F12 ...
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bioRxiv - Neuroscience 2024Quote: ... Retinal tissues were embedded in 3% low-melting agarose (#50100, Lonza) within a plastic cubic mould ...
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bioRxiv - Molecular Biology 2021Quote: ... and 199 (M199) medium (3: 1) supplemented with 10% fetal bovine serum (FBS),10 mM glutamine and penicillin-streptomycin (all from Lonza, Basel, Switzerland) and their purity was verified with the fibroblast marker TE-7 (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... Human primary hepatic stellate cells from donors 3 and 4 were purchased as isolated hepatic stellate cells from Lonza (cat# HUCLS). Donor information is listed below.
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were tested to be negative for mycoplasma every 2–3 months using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Genetics 2020Quote: ... hESCs were nucleofected with 2 μg of CRISPR and 3 μl ssODN (100 μm) using the P3 Primary Cell Kit L (Lonza). hESCs were then plated onto puromycin-resistant (DR4 ...
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bioRxiv - Neuroscience 2021Quote: ... The behavioral assay consisted of a 3% agarose (SeaKem LE Agarose, Lonza) surface coating (50 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... as previously described (3) and the Cell Line Nucleofector Kit R (Lonza). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted an incubated for 3’ with ACK lysis buffer(10-548E, Lonza) to remove red blood cells ...
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bioRxiv - Immunology 2022Quote: ... After 3 days cells were harvested and resuspended in P3 buffer (Lonza), mixed with RNP complexes ...
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bioRxiv - Molecular Biology 2020Quote: ... and the resulting PCR products were analyzed on a 3 % MetaPhorTM (Lonza) agarose gel ...
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bioRxiv - Cancer Biology 2021Quote: ... 3×106 viable cells were resuspended in P3 Primary Cell solution (Lonza), mixed with 2 µM control or 2 µM target-specific ASO and transferred to nucleocuvettes for nucleofection on a 4D-Nucleofector System (Lonza ...
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bioRxiv - Immunology 2021Quote: ... Human Umbilical Venous Endothelial Cells (HUVEC; pooled from 3 individual donors; LONZA) were starved for 6 h ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA samples were loaded on 3% NusieveTM GTGTM Agarose gel (Lonza, 50081), and electrophoresis was run at 100V in 1X TBE buffer for ∼50 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were separated by gel electrophoresis using a 3% MetaPhor agarose (Lonza) using standard techniques ...
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bioRxiv - Biophysics 2023Quote: ... After rinsing 3 times with phosphate buffered saline (PBS, 17-517Q, Lonza), cells were stained with Hoechst 33342 in imaging medium for 15 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Transfection of primary T cells with Cas9 RNP complexes and TCRβα HDR templates was performed 3-4 days following activation using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350 bp ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350bp ...
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bioRxiv - Bioengineering 2021Quote: ... Erythrocytes were lysed for 3 min on ice in ACK lysing buffer (Lonza), stained with biotinylated lineage antibodies against CD3ε (145-2C11) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated by 3% low melting agarose gel (Lonza, cat#50111) and detected with Gel Image System (Tanon 1600) ...
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bioRxiv - Pathology 2024Quote: ... The cells were initially cultured for 3-days in ALI-growth medium (Lonza) based on manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Neonatal human epidermal keratinocytes (NHEK-Neo) pooled from 3 donors (Lonza, Cat. 00192906) were cultured in KBM Gold keratinocyte basal medium (Lonza ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were regularly passaged 2-3 times a week and routinely tested for mycoplasma contamination using MycoAlert Plus mycoplasma Detection kit (Lonza, #LT07-710).
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bioRxiv - Genomics 2020Quote: ... Human umbilical vein endothelial cells were isolated from umbilical cords of healthy donors after cesarean sections (HUVECS; n = 4; RWTH Aachen) (55) or obtained from Lonza (n = 3, Basel, Switzerland) (8) ...
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bioRxiv - Bioengineering 2022Quote: ... Calu-3 cells were maintained in EMEM (with EBSS and L-glutamine, BioWhittaker, Lonza) supplemented with 20% FBS (heat-inactivated ...
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bioRxiv - Bioengineering 2022Quote: ... Human umbilical vein endothelial cells (HUVECS; C2519A) were cultured in EGM-3 medium (Lonza) and were used passages 2-3 for organoids fabrication ...
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bioRxiv - Developmental Biology 2022Quote: A single cut in the NKX2.2 3’UTR was made using transient transfection (Lonza Human Stem Cell Nucleofector Kit VPH-5012 ...
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bioRxiv - Immunology 2023Quote: ... The remaining erythrocytes were lysed with 3 mL ACK lysing buffer (Lonza, Basel, Switzerland) for 5 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Dinucleosomes were purified and excised from a 3% NuSieve GTG agarose gel (Lonza #50081) using Zymoclean Gel DNA Recovery Kit (Zymo #D4008) ...
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bioRxiv - Bioengineering 2024Quote: Primary human hepatocytes from 3 different non-diseased donors were obtained commercially (Lonza, #HUCPI). These were quality-controlled ...