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Citations for Lonza :
651 - 700 of 788 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... For larger scale electroporation in the 1 ml scale of Nucleocuvette Cartridge (Lonza, V4LN-7002), reactions were scaled up 10-fold for cell suspension ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10% fetal bovine serum (GeminiBio, Sacramento, CA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2- betamercaptoethanol (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
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bioRxiv - Genomics 2020Quote: ... S2 cells were seeded at 2×106 per 6cm plate in 4 mL S2 medium (Lonza). One hour after plating ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed and resuspended at 2.5×106/ml in R5 medium (RPMI 1640, Lonza, Switzerland), 5% heat-inactivated FBS (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... murine lymphocytes were resuspended at 3.5×106 cells/mL in supplemented HL-1 media (Lonza, UK) (1% L-glutamine ...
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bioRxiv - Bioengineering 2022Quote: ... 5 × 107 hepatocytes were suspended in 30 mL of HCM™ (Lonza, Sales Ltd, Basel, Switzerland) and injected via the bile duct at a flow rate of 1 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... Exponentially growing Spodoptera frugiperda 9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium #BELN12-730Q) were infected with high-titer Baculovirus suspension ...
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bioRxiv - Evolutionary Biology 2019Quote: ... inactivated trypsin by adding 2 volumes of medium (500 ml DMEM (Lonza, Catalog no. BE12-733F), 55 ml FBS (PAN ...
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bioRxiv - Immunology 2020Quote: ... Antibodies which contained endotoxin levels above 3 EU/ml (Kinetic-QCL Kinetic Chromogenic LAL Assay, Lonza) were depleted of endotoxin with the ToxinEraser Endotoxin Removal kit (GenScript ...
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bioRxiv - Microbiology 2021Quote: ... Agarose-medium was made by autoclaving a 17.6 µg/ml SeaKem ME agarose (Lonza, Basel, Switzerland) dilution and heating it to 65°C ...
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bioRxiv - Bioengineering 2022Quote: ... 20 μL of T cells were resuspended in P3 buffer at 5 × 107 cells/mL (Lonza) and added to the electroporation mixture ...
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bioRxiv - Immunology 2022Quote: ... Absence of endotoxins (< 1EU/mL) was confirmed using the PyroGene™ Recombinant Factor C Assay (Lonza) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and the cell pellet resuspended in 2 ml of ACK lysing buffer (Lonza, Cat# 10-548E). After centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: Primary OCSC cultures were seeded at 5000 cells/ml in MEBM™ (Lonza, cat# CC-3151) supplemented with 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2021Quote: ... We then electroporated 4.0×105 of wildtype or the stably integrated mCherry K562 cell lines in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with pJT396 in combination with an equimolar amount of either pUC19 or a Cp36 expression vector ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Neuroscience 2019Quote: ... the dissociated neurons were centrifuged down to remove the supernatant and resuspended in 80 to 100 μl of Amaxa electroporation buffer for mouse neurons (Lonza Cologne GmbH; Cologne, Germany) with mixtures of siRNA (0.2 nmol per transfection ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 (see sequences of sgRNAs in Table S2) was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Biochemistry 2023Quote: ... Mm.Cas9.NOS2.1.AA) and 1 µM HiFi Cas9 enzyme (IDT, catalog no. 1081060) in 100 µL Nucleofector Solution V plus supplement (Lonza, catalog no. VCA-1003), using the preprogrammed electroporation protocol B-032 on Nucleofector II/2b ...
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bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 K562 cells harboring a monoclonal Kp03 landing pad and multiclonal rTetR-VP48 expression construct were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1000 ng of reporter donor plasmid (pMMH56,59,59) ...
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bioRxiv - Molecular Biology 2019Quote: ... 150,000 cells were resuspended in 20 µL of P3 Primary Cell Nucleofector Solution with Supplement 1 and mixed with the gRNA/rCas9 solutions before electroporation (4D-Nucleofector System, Lonza, Walkersville, MD; program DC-100). For experiments involving the Neon Transfection System (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... PBMCs were then treated with 2 ml of erythrocytes lysis buffer (Lonza Walkersville inc. #120-02-070) for 1 min ...
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bioRxiv - Microbiology 2021Quote: Leukocyte transmigration assay was carried out by addition of 5×106 cells/mL hPBMC (#CC-2702 Lonza) to DENV2-infected HUVEC monolayers at 24 hpi following different treatments ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed with 5 mL of 1X-phosphate buffered saline without magnesium and calcium (PBS) (Lonza) and supplemented with fresh DMEM-HEPES supplemented with 10 mM sodium butyrate (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... EV pellets obtained from approximately 90 mL CM were re-suspended in 150-200 µL PBS (Lonza). Protein concentration was determined with the Bradford assay.
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bioRxiv - Molecular Biology 2020Quote: ... Days 16-24 media contained recombinant Oncostatin M (20ng/ml, Thermo) in HCM media lacking EGF (Lonza).
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bioRxiv - Bioengineering 2023Quote: ... the ground bones in 50 ml tubes were washed with phosphate-buffered saline (PBS, Lonza, Slough, UK) containing 2% penicillin/streptomycin (P/S ...
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bioRxiv - Microbiology 2023Quote: ... siRNAs (1 μg per ml) were nucleofected into ISE6 cells using the 4D-Nucleofector System (Lonza Bioscience). Tick cells were centrifuged at 100 x g for 10 minutes to pellet the cells ...
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bioRxiv - Cell Biology 2019Quote: ... Minced tissue were put into a 15-mL falcon tube filled with 3mL EBM-2MV (Lonza, Cologne, Germany) supplemented with 0.5% FBS ...
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bioRxiv - Bioengineering 2019Quote: ... cell culture surfaces were treated with 20 μg/mL PDL diluted in sterile cell culture sterile water (Lonza) overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10 ng/mL basic fibroblast growth factor or MEGM™ culture medium (Lonza, cat. no. CC-3150), completed as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... at 0.1-1 μg/mL or as a control a GFP plasmid (PmaxGFP, Lonza, Cat. No. D-00059) using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... 0.8×106 CD8+ enriched PBMCs/well were seeded with 1µg/mL soluble αCD28 (clone CD28.2, eBioscience) in 1mL IMDM (LONZA) supplemented with 8% Fetal Calf Serum ...
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bioRxiv - Immunology 2021Quote: ... and suspended at a concentration of 3 × 106cells/mL in RPMI-1640 (BioWhittaker®, Lonza, Walkersville, MD, USA) containing 15% heat-inactivated horse serum ...
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bioRxiv - Bioengineering 2019Quote: ... or γδ-T cells were activated and cultured for 2 days at 0.5 to 1.0 million cells/mL in XVivo15 medium (Lonza) with 5% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2020Quote: ... U–2–OS-pEP15 cells (5) were maintained in 1 mg/ml glucose phenol-red-free DMEM (Lonza) supplemented with steroid-free FBS (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... at a density of 2 million cells/mL in serum-free cell culture medium (Lonza AG, Basel, Switzerland) supplemented with 0.5 μg/mL FMS-like tyrosine kinase-3 (Peprotech ...
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bioRxiv - Microbiology 2022Quote: ... diluted in an equal volume of DMEM containing 20% FBS and 200 U/ml of penicillin/streptomycin (Lonza), and ∼1.5×104 hemolymph sporozoites were added per well ...
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bioRxiv - Microbiology 2022Quote: ... Agarose-medium was made by autoclaving a dilution of 17.6 μg/ml SeaKem ME agarose (Lonza, Basel, Switzerland) and heating it to 65°C ...
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bioRxiv - Pathology 2023Quote: ... and the cell pellet was resuspended in 10 mL of Endothelial Cell Growth Medium-2 (EGM-2, Lonza). The cell suspension was plated into a 75 cm2 tissue culture treated flask and cultured in a 5% CO2 incubator at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-well dishes were seeded with 1.0 × 106 Sf9 cells per well in 2.0 mL InsectExpress medium (Lonza). Cells were transfected with 20 mL of bacmid per well ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated NK cells were activated at 1 x 106 cells mL-1 for 5 days in XVivo15 medium (Lonza) with 5% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... 500 EBs were transferred per T175 tissue culture flasks in 20 mL X-VIVO™15 (04-418Q, Lonza), supplemented with 100 ng mL-1 M-CSF (PHC9504 ...
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bioRxiv - Neuroscience 2021Quote: ... High Five cells were seeded 1 day prior to infection at 1×106 cells/mL in InsectXpress medium (Lonza). Cells were co-infected with low-passage recombinant baculovirus for COL6A3WT or COL6A3ΔC4 along with P4HA1/P4HB at a MOI of 2 and 0.2 respectively ...
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bioRxiv - Immunology 2020Quote: ... Colons were incubated 2 times at 200 rpm in 40 mL HBSS + 0.1% BSA + 1% Penicillin-Streptomycin (PS, Lonza) + 5mM EDTA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells were debeaded with an EasySep magnet (STEMCELL) prior to resuspension and electroporation at 0.5-1×106 cell mL−1 in P3 buffer (Lonza). Following electroporations with RNPs and HDRTs ...
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bioRxiv - Biochemistry 2022Quote: ... the media supernatant was removed and we washed the cells with 2 ml of prewarmed 1xDPBS (Lonza, 17-512F). The PBS was then removed and cells were then incubated at 37°C in 250 μL of trypsin (Corning ...