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Citations for Lonza :
601 - 650 of 788 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 250,000 oligodendrocyte precursors were gently resuspended into 20 ml of nucleofector solution (Lonza P3 Primary Cell 4D-Nucleofector V4XP-3032 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 × 103 cells were plated in low-attachment 96-well plates containing 100 μL MEGM media (without BPE) (Lonza, CC-3150), 20 ng/mL bFGF (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... in 100 μl PBS (GE Lifesciences, Marlborough, MA) and were transfected by electroporation using a 4D-Nucleofector System (Lonza, Walkersville, MD) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5-2×106 cells were pelleted for each condition and resuspended in 100 μL complete nucleofector solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza) to which 1μg of pCR2.1-mClover-LMNAdonor ...
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bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated using Histopaque density 1.077 g/mL (Lonza). CD14+ cells were sorted with the MACS system (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2021Quote: ... 2 mM L-glutamine and 50 units/ml penicillin/streptomycin (cDMEM; Lonza, Slough, UK). Soluble foam proteins were prepared as above ...
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bioRxiv - Bioengineering 2020Quote: ... For transfection 4 × 106 cells/mL cells were resuspended in ProCHO-4 Medium (Lonza) supplemented with 4 mM Ultraglutamine (Lonza) ...
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bioRxiv - Cancer Biology 2022Quote: ... the culture medium was replaced with 2 mL of DMEM-F12 (LONZA #12-719F) supplemented with 10 % FBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Efflux experiments were performed in 6-well plates in 2.5 ml Pro293a medium (Lonza) supplemented with 2 mM L-glutamine and 100 units pen/strep per ml.
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 10% Fetal Bovine Serum (FBS; HyClone) and 50 µg/ml Gentamicin (Lonza). Medium was replaced every two to three days ...
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bioRxiv - Microbiology 2020Quote: ... 1 × 1010 freshly collected conidia were incubated shaking in 50 mL RPMI 1640 (Lonza) for 5 hours (h ...
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bioRxiv - Immunology 2019Quote: ... Cell pellets were then resuspended in 5 mL ACK Lysis Buffer (Lonza, Edinburgh UK), washed and resuspended with DPBS for flow cytometry.
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bioRxiv - Physiology 2022Quote: ... and 2.5 μg/mL amphotericin B in Hank’s balanced salt solution (HBSS) (Lonza, UK). It was then finely minced and digested overnight on a rocker at 4 °C in 1 mg/mL protease XIV (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... the pellets were resuspended in 20 mL of P3 solution with added supplement (Lonza), and 2 mg of the plasmid encoding tandem copies of the LY2 genome (Aldevron ...
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bioRxiv - Microbiology 2022Quote: ... and the well was gently washed once with 5 mL of PBS (Lonza, Rockland) to remove un-adherent floating bacteria ...
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bioRxiv - Immunology 2023Quote: ... The remaining erythrocytes were lysed with 3 mL ACK lysing buffer (Lonza, Basel, Switzerland) for 5 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... containing 0.3 μl/ml SYBR Green I (10,000× in DMSO; Lonza, Cat. No. 50513). Plates were incubated in the dark for at least 1 h at room temperature ...
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bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 µg of PX459 gRNA vector (5’-GACTCCAGTCTTTCTAGAAGA-3’) were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-100) using program A-30 in the XRep ...
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bioRxiv - Genomics 2021Quote: ... Five million cells and the RNP mixture were combined in Lonza cuvettes in a total volume of 100 µl P3 buffer and nucleofected using CX100 program (LONZA, Basel, Switzerland). The cells were plated at a density of 1 × 106 cells / ml of SFEM-II after 5 minutes in recovery media ...
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Genomics 2022Quote: ... 5 million cells were transfected with 10ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit T (Lonza), program X-001 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were electroporated with 10 µg lineage recorder DNA and 1 µg Sleeping Beauty transposase in 100 µl Human Stem Cell nucleofection buffer (Lonza, VVPH-5022) using the B-016 program of the 4D-Nucleofector (Lonza ...
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bioRxiv - Biochemistry 2022Quote: ... For dual sgRNA gene editing 100 pmols of Cas9 RNP with cut site A sgRNA and 100 pmols of Cas9 RNP with cut site B sgRNA were nucleofected (Lonza 4D nucleofector) with CA137 pulse code ...
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bioRxiv - Immunology 2022Quote: 1 × 105 HLFs were mixed with 2 μg of 100 μg/ml of the above expression plasmids in 100 μl PBS (GE Lifesciences, Marlborough, MA) and were transfected by electroporation using a 4D-Nucleofector System (Lonza, Walkersville, MD) following the manufacturer’s protocol CZ-167 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were seeded onto 96-well plates at a density of 3×104 cells per well in 100 μL cell culture media that consisted of DMEM (Lonza, Basel, Switzerland), 1% penicillin–streptomycin (Lonza ...
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bioRxiv - Biophysics 2023Quote: ... The cells were maintained in a 100 mm cell culture dish using SmGM2 Smooth Muscle Cell Growth Medium BulletKit (SmGM2, CC-3182, Lonza, Basel, Switzerland) at 37 °C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... cells then were electroporated using electroporation code EH-100 and using the P3 Primary Cell 96-well Nucleofector Kit (Lonza, V4SP-3096). Knockout pools were maintained for an additional nine days prior to coculturing with H80 feeder cell line with IL-2 (Final conc 20 U/mL ...
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bioRxiv - Immunology 2023Quote: ... 80 µl of supernatant was removed and replenished with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1.0*106 iPSCs were gathered as single cells as described under ‘cell lines and cell culture’ and resuspended in 100 μL NucleofectorTM Solution V (Lonza, Basel, Switzerland) and added to the reaction mixture ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Cell Biology 2020Quote: ... Cells in passage 1 were trypsinized and resuspended (3 × 104 cells/mL) in BEGM** (Lonza, see Table S4 for details of media composition ...
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bioRxiv - Molecular Biology 2021Quote: ... 15 mL of molten normal melting point agarose was applied to GelBond® (Lonza, Switzerland) in a rectangular one-well plate (VWR) ...
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bioRxiv - Neuroscience 2020Quote: ... Red blood cells were lysed with 4 ml of ACK Lysis Buffer (Lonza, Walkersville, MD) per brain ...
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bioRxiv - Neuroscience 2020Quote: ... Mononuclear cells were collected by density centrifugation using lymphocyte separation media (1.077 g/mL, Lonza) as previously described 96 ...
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bioRxiv - Microbiology 2021Quote: ... 10 mL of pre-warmed complete HL-1 medium (HL-1 base medium (Lonza, 344017) supplemented with 20% heat-inactivated FBS (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 0.01 μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) (Lonza, Basel-Stadt, Switzerland) and 1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 0.1 μg/ml of interleukin (IL)-34 (IL-34) (Lonza, Basel-Stadt, Switzerland), 0.01 μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2020Quote: ... culture media supplemented with 0.1μg/ml of inter-leukin (IL)-34 (Lonza, Basel-Stadt, Switzerland) and 0.01μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.01μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) (Lonza, Basel-Stadt, Switzerland) was then added to these cells ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended at 1×106 cells/ml in XH media (X-Vivo 15 (Lonza) with 5% human serum ...
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bioRxiv - Immunology 2022Quote: ... For larger scale electroporation in the 1 ml scale of Nucleocuvette Cartridge (Lonza, V4LN-7002), reactions were scaled up 10 fold for cell suspension ...
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bioRxiv - Immunology 2022Quote: ... Purified B cells were then cultured at 0.5 ×106 cells/mL in RPMI 1640 (Lonza) containing penicillin (100 IU/ml) ...
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bioRxiv - Microbiology 2022Quote: ... at 200 µg/ml and incubated at 37°C overnight in DMEM (Gibco or Lonza) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 3 mL TheraPEAK™ ACK Lysing Buffer (1X) (Lonza, Basel, Switzerland). After a 2 minute incubation ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01 μg/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF) (Lonza, Basel-Stadt, Switzerland) and 1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.1 μg/mL of interleukin (IL)-34 (IL-34) (Lonza, Basel-Stadt, Switzerland), 0.01 μg/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary OCSC cultures were seeded at 5000 cells/ml in MEBM (Lonza, cat# CC-3151) supplemented with 2 mM L-glutamine ...