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Citations for Lonza :
5251 - 5300 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The product was purified and 2 µg were added to the RNP and diluted in 100 µL P3 nucleofection buffer (Lonza). This mixture was nucleofected with 2×106 stimulated human primary T cells using the 4D-Nucleofector (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... with supplements (Lonza, CC-4133) and maintained for no longer than six passages ...
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bioRxiv - Biophysics 2021Quote: ... HUVECs (Lonza, CC-2519) were cultured in Endothelial Cell Basal Medium (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... 0.1 mM non-essential amino acids (Lonza), 1 mM sodium pyruvate (Life technologies ...
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bioRxiv - Biophysics 2021Quote: ... HUVECs (Lonza, expanded to passage 3) were transduced with GFP tagged VE-cadherin (65 ...
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bioRxiv - Biophysics 2021Quote: ... were cultured in Endothelial Cell Basal Medium (Lonza, CC-3121) with supplements (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... for all imaging experiments involving exogenous expression we used the Lonza Amaxa II Nucleofector System with Cell Line Nucleofector Kit V reagent (Lonza VCA-1003). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... were grown in EGM-2 medium (Lonza, Basel, Switzerland). To express progerin in HUVECs ...
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bioRxiv - Biophysics 2021Quote: Commercially available primary human umbilical vein endothelial cells (HUVECs) (pooled, passages 3-5, Lonza, Basel, Switzerland) were grown in EGM-2 medium (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Biophysics 2021Quote: ... 1% Penicillin/Streptomycin and 20% supernatant of M-CSF expressing L-929 cells (Lonza) in untreated six-well culture plates ...
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bioRxiv - Biophysics 2021Quote: ... 1 % L-Glutamine and 20 % supernatant of M-CSF expressing L-929 cells (Lonza).
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bioRxiv - Cancer Biology 2021Quote: ... expressing Cas9 and sgRNA targeting mouse p53 and pmaxGFP plasmid (Lonza). Similarly ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were thawed and rest at 37°C in IMDM (Lonza)/10% nHS for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 2 mM Ultraglutamine I (Lonza), 10 mM HEPES (GIBCO) ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were routinely tested for mycoplasma contamination using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza LT07-710).
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines used in this study tested negative for Mycoplasma contamination via MycoAlert™ PLUS Mycoplasma Detection Kit (LT07-710, Lonza, Basel, Switzerland).
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bioRxiv - Cancer Biology 2021Quote: ... Single nuclei were sorted into 5□μl Pro-Freeze-CDM NAO freeze medium (Lonza)□supplemented with□7.5% dimethyl sulfoxide ...
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bioRxiv - Cancer Biology 2021Quote: ... ARPE-19 cells were maintained in Complete media containing DMEM: F12 supplemented (Lonza; Basel, Switzerland) + 10% FBS + 1% PEST + 2.5 mM L-Glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug solutions were removed and wells washed twice with 1 x phosphate buffered saline (PBS; Lonza). Fresh Complete
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bioRxiv - Cancer Biology 2021Quote: The Human Umbilical Vein Endothelial Cells (HUVEC) were purchased from Lonza (Walkersville ...
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bioRxiv - Cancer Biology 2021Quote: ... with EGM-2MV Single Quots (Lonza, Walkersville, MD, USA) in 25 cm2 gelatinized cell culture flasks at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... batch 0000440546 and 0000442486) and cultured in EBMTM-2 Basal Medium (Lonza, Walkersville, MD, USA) with EGM-2MV Single Quots (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... Reaction products were analyzed on a 1.3% SeaKem GTG agarose gel (Lonza) and imaged with the InGenuis3 (Syngene) ...
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bioRxiv - Biophysics 2021Quote: ... lactis strain NZ9800 by electroporation [42] (Lonza) which were screened on GM17 plate medium with 5 μg/ml Chloramphenicol and further determined their genotypes by sequencing.
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bioRxiv - Biophysics 2021Quote: ... 10 pmol of the cholesterol-modified reverse primer and 1μL 20X SYBR® Green I Nucleic Acid Stain (Lonza) were mixed with the KAPA2G Fast HotStart ReadyMix in a total volume of 20 μL ...
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bioRxiv - Cell Biology 2021Quote: ... containing bone marrow mononuclear cells was washed in 5 ml basal medium (α – MEM containing 10% FBS and 100 U mL−1 penicillin and 100 μg mL−1 streptomycin; Lonza). All washing stages were at 400 x g for 5 minutes.
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bioRxiv - Cell Biology 2021Quote: ... Marrow cells were resuspended in 10 mL α – MEM and subjected to density centrifugation using 20 mL Lymphoprep™ (Lonza) at 800 x g for 20 minutes with no braking on the centrifuge ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 clonal Kp03 landing pad cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Bioengineering 2021Quote: Landing pad cell lines were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with the promoterless mCherry donor containing the matching attP at a dose of either 1000 ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector SF, program FF-120), with 3000 ng LSR plasmid and 2000 ng pseudosite attD plasmid ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 WT K562 were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Bioengineering 2021Quote: 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of a pEF-1α-PuroR-P2A-mCherry donor plasmid containing an upstream Cp36 attD site (pJT371) ...
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bioRxiv - Bioengineering 2021Quote: ... We then electroporated 4.0×105 of wildtype or the stably integrated mCherry K562 cell lines in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with pJT396 in combination with an equimolar amount of either pUC19 or a Cp36 expression vector ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 K562 cells harboring a monoclonal Kp03 landing pad and multiclonal rTetR-VP48 expression construct were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1000 ng of reporter donor plasmid (pMMH56,59,59) ...
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bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of the same Cp36 PuroR-P2A-mCherry donor ...
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bioRxiv - Bioengineering 2021Quote: ... were subsequently integrated into the AAVS1 locus of cells expressing rTetR-VP48 using TALEN-mediated homology-directed repair as follows: 1.0×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T0-16) with 1000 ng of reporter and 500 ng of each TALEN-L (Addgene #35431 ...
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bioRxiv - Bioengineering 2021Quote: ... in α-MEM (Lonza, Basel, Switzerland) supplemented with 5% GMP-grade human platelet lysate (PL ...
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bioRxiv - Neuroscience 2021Quote: ... H9 cells were nucleofected using the Amaxa nucleofector (Lonza) with the P3 primary cell 4D nucleofector kit L (Lonza LONV4XP-3024) ...
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bioRxiv - Neuroscience 2021Quote: ... with the P3 primary cell 4D nucleofector kit L (Lonza LONV4XP-3024). Puromycin (Invivogen 10 mg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Nucleofection was performed using 4D-Nucleofector™ core unit from LONZA. Cells were plated into one well of a 6-w plate after nucleofection in 2 mL of RPMI (10% FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... filled with α-MEM medium (Lonza) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were confirmed to be mycoplasma-free by the MycoAlert Mycoplasma Detection Kits (Lonza).
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bioRxiv - Cancer Biology 2021Quote: ... 10 μg/ml Bovine Pituitary Extract (BPE; Lonza), 10 ng/ml recombinant human IL-8 (CXCL8 ...
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bioRxiv - Cancer Biology 2021Quote: ... Melanoma cell lines were cultured in RPMI-1640 (Lonza) containing 5% FBS (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2.5·104 stably GFP-expressing PC3 cells were added to the osteoblasts and co-cultured in α-MEM medium (Lonza) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and lines were routinely tested for mycoplasma using MycoAlert (Lonza, LT07-318). All cell lines were cultured in high-glucose DMEM (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... washed twice with RPMI (Lonza) and incubated with 3mls red cell lysis buffer (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell lines were mycoplasma-tested (Lonza MycoAlert Plus, LT07-710), used within 10 passages and cultured in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were maintained at 37°C and 5 % CO2 in a humidified atmosphere and routinely tested to be mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza). Cells were cultured no longer than 15 passages before experimental use.