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Citations for Lonza :
5501 - 5550 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... pPBCAG-hph and a PiggyBac Transposase vector using the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001). Transfected cells were selected with 200 μg/ml hygromycin B Gold (Ibian tech. ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×106 EL16.7 TST ESCs were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001) using program A-30 with 1.6 µg each of GFP and tdTomato circularised targeting vectors and 5 µg single gRNA vector PX459 (5’-TATACCTAATCATTATGCCG-3’ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... (Lonza, CAT#12001-798, 10,000x concentrated) and incubated overnight at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and an Amaxa 4D Nucleofector device from Lonza. 5 μg plasmid DNA were added into 5 × 106 suspended cells within the kit provided solution and electroporated using DG-113 program in the device for Caco-2 cells according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... pcDNA3-JUP or empty vector were transfected into Caco-2 cells using SE Cell Line 4D-Nucleofector™ X Kit (Lonza) and an Amaxa 4D Nucleofector device from Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... Spheroids were grown in Mammary Epithelial Basal Medium (Lonza) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s protocol using the program T-20 and the Amaxa kit R (Lonza). Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2×106 fibroblasts were harvested and used in each transfection with a Nucleofector device (Lonza) according to the manufacturer’s protocol using the program T-20 and the Amaxa kit R (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: ... hTERT-HME1 (ATCC CRL-4010) were cultured in Mammary Epithelial Cell Basal Medium (MEBM) supplemented accordingly to manufacturers (LONZA). HCC1954 cells (ATCC CRL-2328 ...
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bioRxiv - Immunology 2021Quote: ... The siRNA duplexes were used to transfect purified human naïve B cells using the Human B Cell NucleofectorTM Kit (VPA-1001, LONZA). Transfected B cells were then stimulated with CpG ODN 2395 plus IL-2 and IL-21 for 96 h before genomic DNA extraction for analysis of Sμ-σδand Sμ-Sγ1 DNA recombination ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 µg of PX459 gRNA vector (5’-GACTCCAGTCTTTCTAGAAGA-3’) were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-100) using program A-30 in the XRep ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µg of pmaxGFP (Lonza) was mixed with 1 µl of Lipofectamine™ Stem Transfection Reagent (STEM00001 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 × 105 organoid single cells were re-suspended with Lonza P3 nucleofection buffer and 1 µl of pmaxGFP (Lonza) and transferred to 20 µl nucleofection cuvette (V4XP-3024 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell suspension was further mixed with pre-formed Cas9 RNPs and equally distributed into two 20 µl cuvettes (V4XP-3024, Lonza). Nucleofection was performed using program EA104 ...
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bioRxiv - Cell Biology 2021Quote: ... 10% EGM2 Endothelial Growth Medium (Lonza, Basel, Switzerland), 20 mM HEPES (Biosera) ...
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bioRxiv - Immunology 2021Quote: ... and endothelial growth medium (Lonza, CC-3202). On day 5 ...
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bioRxiv - Immunology 2021Quote: ... primary human lung microvascular endothelial cells (Lonza, CC-2527, P5) and primary human lung alveolar epithelial cells (Cell Biologics ...
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bioRxiv - Immunology 2021Quote: ... The frequency of cells producing IFN-γ in responses to antigen was quantified with ELISpot (Diaclone; 2B Scientific, Oxon, U.K.) performed in HL-1 serum-free medium (BioWhittaker; Lonza, Slough, U.K.), supplemented with L-glutamine and penicillin–streptomycin (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Cell lines were regularly tested for mycoplasma using the MycoAlert™ Mycoplasma Detection Kit (Lonza).
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bioRxiv - Immunology 2021Quote: ... primary NHBEs (Lonza, Basel, Switzerland) of four genetically independent donors were grown as monolayers in 100% humidity and 5% CO2 at 37 °C in serum-free defined growth media (BEGM ...
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bioRxiv - Immunology 2021Quote: ... of four genetically independent donors were grown as monolayers in 100% humidity and 5% CO2 at 37 °C in serum-free defined growth media (BEGM, Lonza). NHBEs (passage 3 ...
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bioRxiv - Immunology 2021Quote: ... Approximately 15,000 HMEC-1 cells/100 μl were seeded on coated wells using EGM-2 media (Lonza, Switzerland) and incubated overnight either with conditioned media from toxin-treated iPSC-MSC or vehicle at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... cells were rested in basal medium (BEBM, Lonza) for 12 h ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected using the Amaxa HUVEC Nucleofector kit (Lonza) according to the manufacturer’s protocol (2-5 µg plasmid DNA ...
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bioRxiv - Genomics 2021Quote: ... which was performed using the Nucleofactor 2b platform (Lonza) Bacteria program 2 ...
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bioRxiv - Genomics 2021Quote: ... Cell lines were authenticated by STR profiling and mycoplasma testing was performed using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza). U2OS cells were grown in DMEM supplemented with 10% tetracycline-free foetal bovine serum (Clontech) ...
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bioRxiv - Immunology 2021Quote: ... Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit; Lonza). After nucleofection ...
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bioRxiv - Immunology 2021Quote: ... The P14 cell/Cas9/RNP mixes were transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Immunology 2021Quote: ... 1-10 million activated P14 cells were re-suspended in 20 μL primary cell nucleofection solution (Lonza), then mixed and incubated with the crRNA/Cas9 mix for 2 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Stimulation with live bacteria was carried out in medium without antibiotics and gentamycin (Lonza) was added to a final concentration of 50 µg/ml after 3 h ...
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bioRxiv - Immunology 2021Quote: ... for exECs (Program M-003, Nucleofector 2b, Lonza), and using the GPR39 siRNA Silencer Select purchased from Thermo ...
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bioRxiv - Immunology 2021Quote: ... Silencing of the zinc receptor Gpr39 was performed by electroporation using Nucleofector electroporation kit (VPI-1001, Lonza) for exECs (Program M-003 ...
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bioRxiv - Immunology 2021Quote: ... 100 units/mL penicillin-streptomycin (Lonza, Milano ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 2mM L-glutamine (Lonza, Milano, Italy), 100 units/mL penicillin-streptomycin (Lonza ...
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bioRxiv - Immunology 2021Quote: ... Absence of mycoplasma contamination was confirmed using the MycoAlerta Plus mycoplasma detection kit (Lonza #LT07705). Culture supernatants were collected 5 – 7 days post-transfection by centrifuging the culture at 4,000 × g for 25 min ...
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bioRxiv - Immunology 2021Quote: ... NIH) in 100 µl IMDM/Glutamax/10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Immunology 2021Quote: ... 1 million cells were suspended in 30ul of EP buffer (Lonza) with 3μg of either pcDNA3-FLAG-HA (EV ...
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bioRxiv - Immunology 2021Quote: ... density gradient centrifugation (20 min, 800g) and culture for 12-18 h in X-VIVO 10 TM medium (Lonza) supplemented with gentamicin ...
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bioRxiv - Immunology 2021Quote: ... Plasmids expressing individual guides were transfected into HEK293T by Amaxa nucleofector and Nucleofector® Kit V (Lonza). After 48 h ...
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bioRxiv - Immunology 2021Quote: ... Erythrocytes were lysed briefly using ACK lysis buffer (Lonza) with 1mL per spleen for 1∼2 mins before adding 10 mL PBS containing 2% FBS to restore iso-osmolarity ...
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bioRxiv - Immunology 2021Quote: ... Isolated SNECs cells were grown in Bronchial Epithelial cell Growth Media (BEGM bullet kit, Lonza). At 80% confluency ...
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bioRxiv - Immunology 2021Quote: ... The remaining tissue was minced and digested with stirring for 60 min in complete RPMI (RPMI1640; Lonza; supplemented with 10% fetal calf serum (FCS, 1% Pen/Strep; Lonza) containing 0.25 mg/ml Liberase TL (Roche ...
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bioRxiv - Immunology 2021Quote: ... The remaining tissue was minced and digested with stirring for 60 min in complete RPMI (RPMI1640; Lonza; supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Bioengineering 2021Quote: ... were obtained from Lonza and cultured in EBM-2 medium (Lonza) supplemented with penicillin-streptomycin (Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... and EGM-2 SingleQuot bullet kit (Lonza). For imaging experiments ...
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bioRxiv - Immunology 2021Quote: ... Cell lines were routinely mycoplasma-tested using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Immunology 2021Quote: ... Vero cells were acquired from the American Type Culture Collection (ATCC #CCL-81; Manassas, VA, USA) and cultured in DMEM (Lonza, Walkersville, MD, USA) containing 2% FBS (SAFC Biosciences ...
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bioRxiv - Immunology 2021Quote: ... 100 μM non-essential amino acids (Lonza), 1 mM sodium pyruvate (VWR) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 μg of the donor ssDNA and 3 μg of a puromycin resistance plasmid (pCAG-puroR) using the Mouse ES Cell Nucleofector™ Kit (Lonza, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... coated dishes (5 ng/cm2) and cultured in Endothelial Growth Medium 2 (EGM2, Lonza) supplemented with 50 ng/mL VEGF with medium changes every other day until they reached confluency ...