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Citations for Lonza :
1 - 50 of 9698 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Cultures were routinely tested for mycoplasma using the MycoAlert Mycoplasma Detection kit (Lonza; Cat#LT07-318).
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bioRxiv - Neuroscience 2024Quote: ... was slowly placed on top of the solution after cleaning the coverslip with RNAse AWAY (Molecular BioProducts) and 70% ethanol and coating the facing-down side of the coverslip with Gel Slick Solution (Lonza). The tissue was incubated at room temperature for 1.5 h to allow the gel to polymerize ...
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bioRxiv - Physiology 2024Quote: ... by tankblot for one 1.15 h and 100 V (ProSieve EX Transfer buffer 10x, diluted in H2O to 1x, Lonza). Membranes were blocked with 5 % milk powder (MP ...
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bioRxiv - Neuroscience 2024Quote: ... and the nucleofection reaction was prepared using the Cell Line Nucleofector™ Kit V (Lonza; VCA-1003) as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Electroporation was performed with the 4D-Nucleofector System (Lonza) using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza ...
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bioRxiv - Physiology 2024Quote: ... The presence of mycoplasma infection was regularly controlled using the MycoAlert Mycoplasma Detection kit (Lonza).
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bioRxiv - Pathology 2024Quote: HASMCs were purchased from Lonza (CC-2571, Lonza). The HASMCs were maintained in SmBMTM Basal Medium (CC-3181 ...
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bioRxiv - Pathology 2024Quote: ... The HASMCs were maintained in SmBMTM Basal Medium (CC-3181, Lonza) supplemented with SmGMTM-2 SingleQuotsTM supplements (CC-4149 ...
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bioRxiv - Pathology 2024Quote: HASMCs were purchased from Lonza (CC-2571, Lonza). The HASMCs were maintained in SmBMTM Basal Medium (CC-3181 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then electroporated using a Lonza 4D-Nucleofector® X Unit (Lonza #AAF-1003X) with code EH-115 ...
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bioRxiv - Synthetic Biology 2024Quote: ... T cells were cultured in human T cell medium (hTCM) consisting of X-VIVO 15 (Lonza #04-418Q), 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165 ...
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bioRxiv - Synthetic Biology 2024Quote: Electroporation was done 7 days after stimulation by DynaBeads using a P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza #V4XP-3012). 750 ng of HDR template was mixed with 50 pmol of RNP and incubated at room temperature for 10 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... and Jurkat cell lines tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza Cat #LT07-318).
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bioRxiv - Neuroscience 2024Quote: ... digested for 3 min at 37 °C in Accutase and then resuspended in 60 μL nucleofection buffer from the Human Stem Cell Nucleofector™ Kit 2 (Lonza). The suspension was combined 2 μM Electroporation Enhancer (IDTDNA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the cell pellet was resuspended in Keratinocyte Growth Medium-2 Bullet Kit (KGM2 medium) consisting of KGM-2 Basal Medium and KGM-2 SingleQuots Supplements (Lonza, Basel, Switzerland), and then seeded onto Petri dishes coated with collagen IV ...
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bioRxiv - Molecular Biology 2024Quote: ... and received biweekly medium exchanges of serum free XVIVO-10 medium (Lonza #(BE) BP04-743Q ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... SeaKem agarose was purchased from Lonza (Basel, Switzerland). Lipidex-5000 slurry in methanol was purchased from Perkin Elmer Inc (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Dulbecco’s Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI) used to culture cells were obtained from Lonza (NSW, Australia). GlutaMax ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The human monocyte THP-1 cells were cultured in RPMI 1640 media from Lonza (NSW, Australia), containing 4.5 g/L D-glucose and supplemented with 2 mM GlutaMax ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) from Lonza (NSW, Australia), containing 4.5 g/L D-glucose and supplemented with 2 mM l-GlutaMax ...
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bioRxiv - Cell Biology 2024Quote: ... and containing MycoZap plus (VZA-2012; Lonza) at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... with the P3 Nucleofector kit (Lonza, Cat. # V4SP-3096). Naïve murine CD8+ T cells (2 x 106 ...
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bioRxiv - Cell Biology 2024Quote: ... electroporation was performed using a 4D-Nucleofector (Lonza, Cat # AFF-1002B) with the P3 Nucleofector kit (Lonza ...
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bioRxiv - Microbiology 2024Quote: ... Complete DMEM was prepared as follows: Dulbecco modified Eagle medium (DMEM; Lonza, Cat# 12-614Q supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... normal human bronchial epithelial (Lonza), and porcine primary nasal epithelial (24 ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were run on 2.2% agarose gels (Lonza 57031) and imaged using Philips VLounge software ...
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bioRxiv - Systems Biology 2024Quote: ... in Eagle’s Minimum Essential Medium (Lonza, 12-125Q) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2024Quote: ... 2 mM l-glutamine (Lonza, BE17-605E), and 1% penicillin (100 U/ml ...
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bioRxiv - Systems Biology 2024Quote: ... cultured cells were washed by dispensing and aspirating 37°C HEPES buffered saline solution (Lonza, CC-5022) and then dissociated with 0.025% Trypsin/EDTA (Lonza ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2×106 cells were resuspended in P3 buffer (Lonza), mixed with RNP(s) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and added to a 96-well nucleofection plate (Lonza). RNP amounts per electroporation varied based on the number of loci targeted ...
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bioRxiv - Bioengineering 2024Quote: ... MCF 10A cells (ATCC, USA) were cultured in mammary epithelial growth medium (MEGM; Lonza, Switzerland) supplemented with 100 ng/ml of cholera toxins (List Biological Laboratories ...
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bioRxiv - Bioengineering 2024Quote: ... and SK-BR-3 cells (Korean Cell Line Bank, Korea) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Switzerland) supplemented with 10 % (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... the cell culture medium was aspirated and cells were washed with phosphate-buffered saline (PBS; Lonza, Switzerland) followed by cultured in an exosome-production medium for 48 hours ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 10% fetal bovine serum (ATCC) and 0.2% MycoZap Plus-CL antibiotics (Lonza, catalog # 195261). Cell lines were routinely tested for mycoplasma and were periodically authenticated to its early passage using the GenePrint 10 System (Promega ...
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bioRxiv - Immunology 2024Quote: ... Lysis of red blood cells was performed using 300 μl of Ammonium-Chloride-Potassium (ACK) buffer for 1.5 minutes (Lonza, Basel Switzerland). Blood was collected in EDTA-coated tubes (MiniCollect ...
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bioRxiv - Systems Biology 2024Quote: The reporter cell line for the PTGR screen was created by nucleofection of haploid AN3-12 mESCs with 500ng of the reporter construct and 10 µg of a Tol2 transposase encoding plasmid using the Mouse ES Cell Nucleofector Kit (Lonza) according to the manufacturer’s protocol using an Amaxa Nucleofector (Lonza) ...
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bioRxiv - Systems Biology 2024Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and placed in a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC-5002); this was repeated to maximize cell collection ...
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bioRxiv - Systems Biology 2024Quote: ... and adherent cells were expanded with bronchial epithelial cell growth medium (Lonza, CC-3170) and then transferred to a collagen-coated transwell plate (Corning ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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bioRxiv - Systems Biology 2024Quote: ... and then dissociated with 0.025% Trypsin/EDTA (Lonza, CC-5012) for 10 min at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... negative selection of T cells was performed using the EasySep human T cell isolation kit (Stemcell) and cultured in X-VIVO 15 medium (Lonza) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Synthetic Biology 2024Quote: ... resuspended in 20 uL of P3 nucleofection buffer (Lonza) containing 1.2 uL of Cas9 RNP mix and 0.4 ug double-stranded DNA HDR template and electroporated using the EH-115 program ...
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bioRxiv - Neuroscience 2024Quote: ... or electroporation with an Amaxa Nucleofector 2b device (AAB-1001, Lonza), all following manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Absence of mycoplasma was assessed every month using the MycoAlert mycoplasma detection kit (LT07-318, Lonza). HEK293 cells were transfected by electroporation with plasmids coding for either Snap-tagged human mGlu2 receptors (STmGluR2 ...
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bioRxiv - Neuroscience 2024Quote: ... 5 x 105 to 1 x 106 cells were resuspended in 20µL of nucleofection solution (P3 Primary Cell 4D-Nucleofector™; Lonza) and nucleofected (Lonza 4D nucleofector TM Core + X Unit ...
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bioRxiv - Neuroscience 2024Quote: ... and nucleofected (Lonza 4D nucleofector TM Core + X Unit ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC lines were validated by Array Comparative Genomic Hybridization assessment of karyotype and copy number variation (Cell Line Genetics) and confirmed to be sterile and pluripotent via MycoAlert testing (Lonza), Pluritest Array Analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... Isolated T cells were cultured at an initial density of 106 cells/mL in X-Vivo 15 medium (Lonza) supplemented with human serum (5% ...
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bioRxiv - Genomics 2024Quote: Human umbilical vein endothelial cells (HUVECs) were obtained from Lonza (catalog #: CC-2517-0000315288). Cells were cultured at 37°C with 5% CO2 and seeded at 5,000 cells/cm2 ...