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Citations for Active Motif :
351 - 400 of 1695 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-H3K9me2 (1:250; AM39041; Active Motif), rabbit anti-Phospho-Rpb1 CTD (Ser2 ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies used: mouse anti-H3K27ac (1:250; 1 h; 39085, Active Motif) rabbit anti-H3K27Ac (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ChIP against histone H3.3 was performed using the ChIP-IT kit (Active Motif) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA regions of interest were isolated using 5 µg of antibody against MITF (Active Motif, cat# 39789, lot# 11313002). Complexes were washed ...
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bioRxiv - Cancer Biology 2024Quote: ... and adapter ligated using the ATAC-seq Kit (53150) according to the manufacturer’s instructions (Active Motif). Sequencing was done by Azenta Life Sciences and reads were trimmed using the fastp command in fastp v.0.23.4 ...
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bioRxiv - Cell Biology 2024Quote: ... 750 ng spike-in Drosophila chromatin and spike-in antibody (Active Motif, #61686) were added to each reaction before overnight incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-H3K27me3 and anti-H3K27Ac antibody (Active Motif 39156 ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclei were isolated and incubated overnight with Concanavalin A beads and 1.3 µl of the primary anti-H3K27me3 or anti-H3K27Ac antibody (Active Motif 39156 ...
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bioRxiv - Cancer Biology 2024Quote: Bone marrows of drug treated animals were subjected to CUT & Tag analysis by Active Motif (Carlsbad, CA, USA). Analytes were prepared as previously described with modifications (25) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR amplification and barcoding (see Active Motif CUT&Tag kit ...
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bioRxiv - Biochemistry 2024Quote: ... RNAPII (Active Motif 39497, 1:500), SRSF1 (ThermoFisher 32-4600 ...
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bioRxiv - Cancer Biology 2024Quote: 10-100nM of recombinant GST-SETD8 protein were incubated with 0.5μg of recombinant mononucleosomes (H3.1, Active Motif #81070) in methyltransferase buffer (30mM Tris (pH 8.0) ...
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bioRxiv - Cancer Biology 2024Quote: ... and cytoplasmic fractions were extracted using the Nuclear Extract Kit (Active Motif; no. 40010) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... histones from A172 cells treated with scramble and ZNF638 siRNA were purified using a histone extraction kit (Active Motif) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear and whole-cell extracts were prepared from 1.5×107 cells per condition with and without DOX treatment for 4 d using the Nuclear Extract Kit (Active Motif). Co-immunoprecipitation was performed with the nuclear fraction using the Dynabeads Co-Immunoprecipitation Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitation was performed using antibodies against RNA Pol2 (4H8, Active Motif), MAX (10426-AP from Proteintech ...
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bioRxiv - Cancer Biology 2024Quote: ... 5% of soluble chromatin was kept for input DNA and rest of chromatin was used to perform immunoprecipitation with 7.5μg of H4K20me1 antibody (Active Motif #39727) overnight at 4°C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.5μg of recombinant GST-SETD8 proteins were incubated with 1μg of recombinant mononucleosomes (H3.1, Active Motif #81070) and glutathione-sepharose beads (Sigma-Aldrich GE17-0756-01 ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies against H3K27Ac (Active Motif, Cat# 39133, 5 µg per sample), H3K27me3 (Millipore Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-H3K27me3 and anti-H3K27Ac antibody (Active Motif 39156, 23254116-11; Active Motif 39135, 23061102-11).
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bioRxiv - Cancer Biology 2024Quote: ... and nuclei were isolated and incubated overnight with Concanavalin A beads and 1.3 µl of the primary anti-H3K27me3 or anti-H3K27Ac antibody (Active Motif 39156, 23254116-11; Active Motif 39135, 23061102-11) per reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... ATAC-sequencing was done according to standard protocol followed by Active Motif.
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bioRxiv - Developmental Biology 2024Quote: ... tagmentation and DNA purification were performed on 50-100x103 cells/sample using ATAC-Seq Kit (Active Motif) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM EDTA) containing a 1:50 dilution of rabbit primary antibody (anti-H3K4me1 (Active Motif; 39298) or anti-H3K27ac (Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were sequenced by Active Motif as single-end 75-mers on an Illumina NextSeq 500 instrument.
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bioRxiv - Developmental Biology 2024Quote: ... using 18 μg of sheared chromatin from 30 pooled embryonic testes and 5 μl of COUP-TFII antibody (Active Motif, CA 61213). Libraries were sequenced by Active Motif as single-end 75-mers on an Illumina NextSeq 500 instrument.
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202; FLAG, Sigma, F1804; H3K27ac, Active Motif, 39133 ...
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bioRxiv - Developmental Biology 2024Quote: ... 500 ng of histone H3K27ac antibody (Active Motif, 39133) was used for each immunoprecipitation reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... or 0.5 μl PG-Tn5 (Vazyme, S612) and Pol II antibody (Active Motif 102660) were added to freshly prepared DB1 buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µg of spike-in antibody (Active Motif, 61686) were included in the protocol ...
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bioRxiv - Developmental Biology 2024Quote: Chromatinization was performed by Chromatin Assembly Kit from Active Motif (Cat #: 53500). The HeLa core histones in the kit were replaced with equal amounts of recombinant histones from NEB (Cat# ...
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bioRxiv - Developmental Biology 2024Quote: ... ATAC-seq library preparation was performed using the Active Motif kit (Active Motif, cat. 53150) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: ATAC-seq was performed using the Active Motif ATAC-seq kit (Active Motif 53150).99 Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM EDTA) containing primary antibody (anti-H3K27me3 (Active Motif; 39157; dilution 1:50), anti-H3K4me3 (Active Motif ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-H3K4me3 (Active Motif; 39160; dilution 1:50), anti-IgG negative control antibody (EpiCypher ...
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bioRxiv - Cell Biology 2024Quote: ... before the beads were captured on a magnetic rack (Active Motif) and added to the lysates for incubation at 4°C overnight under rotation ...
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bioRxiv - Cell Biology 2024Quote: ... 30 μl protein-G magnetic beads (Active Motif) and 10 μg CTCF antibody (Active motif ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 μg CTCF antibody (Active motif, 61311) were incubated for 2 h at 4°C on a rotor ...
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bioRxiv - Cell Biology 2024Quote: ... after which CTCF protein-RNA complexes were immunoprecipitated using the relevant antibody (anti-CTCF, Active Motif 61331). In parallel to the IP ...
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bioRxiv - Developmental Biology 2024Quote: ... Co-immunoprecipitation was performed 48 h later using Nuclear Complex Co-IP kit (Active Motif #54001) according to kit instruction ...
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bioRxiv - Cell Biology 2024Quote: ... The denatured DNA was subjected to immunoprecipitation with 5-Methylcytosine antibody (Active Motif, Catalog #61479) by incubating the reaction mixture at 4°C for 15 hours ...
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bioRxiv - Genetics 2024Quote: ... or mouse-anti Cas9 (1:1,000, Active Motif, AB_2793684) in 1% milk TBS-T with gentle rocking ...
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bioRxiv - Genetics 2024Quote: ... BC kit (Active motif, Belgium), Methylamp DNA modification kit (Epigentek ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-histone H3-pT3 (1:100, Active Motif, cat# 39154), sheep polyclonal anti human-BUB1 antibody ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-histone H2A-pT120 (1:2000, Active motif, cat# 39391), mouse anti-human HEC1 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with Maxblock Blocking Medium (Active Motif) at room temperature for 2 hours ...
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bioRxiv - Genetics 2024Quote: ATAC-Seq was performed using the ATAC-Seq Kit from Active Motif (Active Motif, 53150) using manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ATAC-Seq was performed using the ATAC-Seq Kit from Active Motif (Active Motif, 53150) using manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The membranes were then probed with H3K27me1(Active Motif: 61015) or H3K27me3 (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-H3K4me3 (Active Motif, 61379) antibodies ...