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Citations for Active Motif :
351 - 400 of 1680 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Histone H3K27me3 (Active Motif 39055, 3µg), Histone H3K36me3 (Abcam ab9050 ...
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bioRxiv - Microbiology 2023Quote: ... sonicated samples were chromatin-immunoprecipitated using the ChIP-IT PBMC kits (Active Motif), according to the manufacturer’s instructions (38) ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl. 5 nM of full length purified ORC1 (Cusabio Catalog N. CSB-BP621667HU(A4) and H2A.Z (Active Motif, Cat. No. 81072) proteins were incubated with 1.1 nM of 32P-labeled single-stranded (ss ...
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bioRxiv - Molecular Biology 2023Quote: One μg of histone H3 peptides with or without H3K4me2 (a.a. 1-21, Active Motif) were incubated with 1 μg of purified GST-PHF21A protein fragments in binding buffer (50 mM Tris/Cl at pH 7.5 ...
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bioRxiv - Physiology 2023Quote: ... Tissues were homogenized using a glass dounce homogenizer (Active Motif) and kept on ice during homogenization ...
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bioRxiv - Microbiology 2023Quote: ... immunoprecipitated with either anti-CTCF (Active Motif 61311) or IgG control antibodies (agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K9me3 (Active Motif, 39162); anti-GST (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and 2µg of 5-hmC (39791, Active Motif, Carlsbad, USA)) ...
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bioRxiv - Genomics 2023Quote: ... Samples were then processed according to manufacturer’s instructions (Active Motif). Samples were paired-end sequenced to obtain more than 5×106 reads ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif, Anti H3 #9715 Cell Signaling), detection protocols have been summarized in Supplemental Table 4 ...
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bioRxiv - Genetics 2023Quote: ... Antibody incubation (HA, ab9134, Abcam, Cambridge, CB2 0AX, UK; Histone H3.3, 91191, Active Motif, Carlsbad ...
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bioRxiv - Developmental Biology 2023Quote: ... Rabbit anti-H3K9me3 (1:500; Active Motif, AB_2532132), Mouse anti-H3K27me3 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Reporters and mimics/inhibitors were reverse transfected with Lipofectamine 3000 (Fisher) and luminescence read after 48 hours with the LightSwitch™ Luciferase Assay Kit (Active Motif).
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bioRxiv - Immunology 2023Quote: ... Control primers were purchased from Active Motif: Hoxc10 (Cat# 71019 ...
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bioRxiv - Genomics 2023Quote: ... incubated for 45 min at RT with primary antibody diluted in 1X PBS/1% BSA (H3K27me3, Active motif-61017 ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 1 (Active Motif, #71001), Human Negative Control Primer Set 3 (Active Motif ...
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bioRxiv - Genetics 2023Quote: ... chromatin was sonicated for 30 cycles (30 s on, 30 s off, 25% amplitude) using an EpiShear probe sonicator (Active Motif) and DNA samples were purified using AMPure XP SPRI beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2023Quote: ... H2A.Z (Active Motif, 39013, 1:50 dilution) was used as a positive control to confirm a successful experiment and a secondary antibody anti-Mouse IgG (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then once with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit). Remove CUT&Tag Wash Buffer ...
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bioRxiv - Genomics 2023Quote: ... rabbit polyclonal anti-CTCF (1:500; Active motif 61311); rabbit polyclonal anti-H3K27me3 (1:1,000 ...
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bioRxiv - Genomics 2023Quote: ... rabbit polyclonal anti-CTCF (1:500; Active motif 61311); rabbit polyclonal anti-EZH2 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Chromatin immunoprecipitation (ChIP) was carried out using the ChIP-IT Express Enzymatic kit from Active Motif (Rixensart, Belgium). In brief ...
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bioRxiv - Systems Biology 2023Quote: ... and/or Anti H3K4me1 1:1000 (Active Motif Cat No. 61633). Antibodies were detected using the following secondary antibodies for their appropriate hots ...
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bioRxiv - Molecular Biology 2023Quote: ... MLL1 complex was purchased from Active Motif (catalog #31423).
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bioRxiv - Cell Biology 2023Quote: ... Phospho-Histone H2A.X (Ser139) (Millipore Cat#05-636, RRID:AB_309864, Active Motif Cat#39117, RRID:AB_2793161), HA-probe (F-7 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of chromatin supplemented with 10 ng of Spike-in chromatin (Active Motif) were used per ChIP experiment ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: H2A.Z (#39114, Active Motif), Sucrase-Isomaltase (sc-393470 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 µg of Spike-in antibody (Active Motif), were used.
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bioRxiv - Cancer Biology 2023Quote: ... Samples were sonicated for four cycles with 30 second intervals at 40% amplitude in freezer-chilled sonication blocks (ACTIVE MOTIF) to an average fragment size of 200-400 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... were sonicated for four cycles with 30 second intervals at 40% amplitude in freezer-chilled sonication blocks (ACTIVE MOTIF) to an average fragment size of 400-800 bp ...
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bioRxiv - Genomics 2023Quote: ATAC-Seq was performed with the ATAC-Seq kit (Active Motif 53150) accordingly to manufactureŕs instructions and samples were sequenced on a NovaSeq device.
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bioRxiv - Cell Biology 2023Quote: ... Immune complexes were collected using 20 μL of protein G magnetic beads (Active Motif). After elution from the beads ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin for re-ChIP was prepared using the ChIP-IT High Sensitivity Kit (Active Motif, catalog no. 53040), as described above ...
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bioRxiv - Molecular Biology 2023Quote: Re-ChIP was performed using the Re-ChIP-IT Kit (Active Motif, catalog no. 53016) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq)59 was performed from HeLa cells using the ChIP-IT High Sensitivity Kit from Active Motif (reference 53040) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Microbiology 2023Quote: ... and total DNA tagmented and purified using the ATAC-seq kit (Active Motif) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... 75uL of Dynabeads were washed and antibodies (5uL anti-RING1B, Active Motif 39663 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and mouse anti Cas9 (1:500, Active Motif). After incubation with secondary antibodies (donkey anti goat Alexa 488 (1/800 ...
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bioRxiv - Neuroscience 2023Quote: anti-5hmC (rabbit, 1:500, Active Motif 39769).
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bioRxiv - Molecular Biology 2023Quote: ... A H3K56ac-specific antibody (Active Motif #39082) was used to detect the respective bands ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ extensions (italic) matching HindIII digested pAM_1C empty destination vector (cat. no 53023, Active Motif) was added to each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were DNase treated and lysed in ice-cold ATAC seq lysis buffer from the ATAC-Seq Kit (Active Motif, Carlsbad, CA, USA) for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 5hmC (1:250, Active Motif, 39769); γH2AX (Ser 139 ...
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bioRxiv - Molecular Biology 2023Quote: ... Open chromatin was profiled through a commercial service (Active Motif, Carlsbad, CA, USA) based on a method previously described [90] ...