Labshake search
Citations for Active Motif :
301 - 350 of 1695 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... or anti-H3K27ac (39133, Active Motif) antibodies ...
-
bioRxiv - Genomics 2024Quote: ... rabbit anti-H3K27ac (39133, Active Motif), and the CUT&Tag-IT assay kit (53160 ...
-
bioRxiv - Genomics 2024Quote: ... and the CUT&Tag-IT assay kit (53160, Active Motif) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... CUT&Tag was performed on 50,000 nuclei by using rabbit anti-H3K9me3 (39765, Active Motif) or H3K27ac and CUTANA CUT&Tag Kit (14-1101 ...
-
bioRxiv - Genomics 2024Quote: ... were bound with antibodies of interests (H3K27ac, Active Motif 39133; H3K4me3, Active Motif 39915; H3K9me3, Abcam ab176916 or H3K27me3, Active Motif 39155) and incubated at 4°C for 36 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... we used Drosophila S2 spike-in normalization strategy and followed the protocol from Active Motif.
-
bioRxiv - Biophysics 2024Quote: For commercially purchased FLAG-tagged co-As (full-length P300 (Active Motif, 81158) and BRD4’s BRD domain (Active Motif ...
-
bioRxiv - Biophysics 2024Quote: ... and BRD4’s BRD domain (Active Motif, 81155)) ...
-
bioRxiv - Genomics 2024Quote: CUT&Tag was performed using a commercial kit from EpiCypher and a polyclonal anti-H3K9me3 antibody (Active Motif). This antibody was selected after preliminary CUT&Tag experiments with low sequencing depth revealed it to yield high concentrations of mononucleosome-size fragments ...
-
bioRxiv - Genomics 2024Quote: ... the assay was performed as described by the manufacturer (Active Motif cat. no. 55017). Briefly ...
-
bioRxiv - Genomics 2024Quote: For anti-CENP-A CUT&Tag library preparation was used the CUT&Tag-IT kit from Active Motif (Cat. No. 53160). Each experiment was performed for 500,000 fresh cells ...
-
bioRxiv - Genomics 2024Quote: ... H3K9me2 (Active Motif, 39239), H3K27me3 (Diagenode ...
-
bioRxiv - Genomics 2024Quote: ... rabbit-H3K4Me3 (Active Motif; 39159, used at 1:500 dilution); rabbit-TrxR2 (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ... Each sample was pooled from 5 retinas (from 5 mice) and 1000 to 5000 cells collected and used for ATAC- seq analysis with the ATAC-seq kit from Active motif (Cat: 13150). Briefly ...
-
bioRxiv - Genomics 2024Quote: ... and 3 µL H3K27ac antibody (Active Motif, 39133) were added to the samples ...
-
bioRxiv - Genetics 2024Quote: ... 100,000 cells from each sample were pelleted and stored frozen prior to ATAC-seq (Active Motif). Illumina NextSeq500 sequencing was used to generate paired-end 42 bp sequencing reads that were mapped to the mm10 genome build using the BWA algorithm with default settings (44) ...
-
bioRxiv - Genomics 2024Quote: ... and next-generation sequencing on an Illumina NovaSeq instrument was performed by Active Motif.
-
bioRxiv - Genomics 2024Quote: ... and next-generation sequencing on an Illumina NovaSeq instrument was performed by Active Motif.
-
bioRxiv - Genomics 2024Quote: ... ab181557),Nanog (Active Motif, 61419),Sox2 (Cell Signaling Technology ...
-
bioRxiv - Genomics 2024Quote: ... We performed immunoprecipitation with a cross-species reactive H3K27ac antibody (Active Motif 39133). The samples were incubated with the antibody overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2024Quote: ... we used Drosophila S2 spike-in normalization method and followed the protocol from Active Motif. ChIP–seq libraries were prepared from 3–5 ng of ChIPed DNA using NEBNext Ultra II DNA Library Prep Kit (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... The suspension was sonicated for 2 min (30” on, 30” off) using a probe sonicator (Active motif), at 85% amplitude ...
-
bioRxiv - Cancer Biology 2024Quote: ChIP assays were performed using ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit following the manufacturer’s protocol (Active motif, France) and 4 × 106 cells per sample and per epitope ...
-
bioRxiv - Cancer Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 15 ug of purified rabbit polyclonal YAP-specific antibody (US Biologicals, Y1200-01D) or rabbit IgG (control) and protein G magnetic beads (Active Motif). Immunoprecipitated chromatin-protein complexes were washed extensively with RIPA buffer (1x ...
-
bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitated chromatin-protein complexes were washed extensively with RIPA buffer (1x, Active Motif) and then with 100 mM ammonium bicarbonate (Merck ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclei were sonicated on ice in ChIP buffer (custom, proprietary to Active Motif) using an EpiShear Probe Sonicator (68% pulsed sonication ...
-
bioRxiv - Cancer Biology 2024Quote: ... Chromatin was isolated from formaldehyde-fixed cell pellets by the addition of lysis buffer (custom, proprietary to Active Motif), followed by cell disruption with a Dounce homogenizer (15 ml volume with tight-fitting pestle ...
-
bioRxiv - Cancer Biology 2024Quote: ... incubated overnight at 4 °C with 1: 1000 primary antibody diluted in blocking solution (H3K9me3, Active Motif; H3, Santa Cruz) followed by secondary antibody diluted 1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Primary antibody used: H3K9me3 (#39161, Active Motif), yH2AX (gtx127342 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The Active Motif ATAC-seq Kit (Active Motif, 53150) was used to perform the ATAC and library prep with an initial live cell input of 100,000 according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... cells were incubated with 0.5µg/100μl of anti-CTCF antibody (Active Motif 61311) in DWB at 4°C for 2hr ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331 ...
-
bioRxiv - Systems Biology 2024Quote: ... to which 10 ng of Spike-In Chromatin from Schneider’s Drosophila Line 2 (S2) (Active Motif) was added ...
-
bioRxiv - Developmental Biology 2024Quote: ... Two independent ChIP-seq experiments were performed by Active Motif, Inc. ...
-
bioRxiv - Developmental Biology 2024Quote: ... 120 ng of spike-in chromatin (Active Motif, 53083) and 2 µg of spike-in antibody (Active Motif ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were then blocked in 5% milk/TBST for 1 h followed by primary antibody incubation at 4 °C overnight at dilution of 1:10,000 rabbit anti hmC (catalog no. 39069, Active Motif). The blots were washed with TBST followed by incubation with secondary 1:3,000 anti-rabbit-HRP for 5-hmC for 1 h ...
-
bioRxiv - Genetics 2024Quote: ... An equal amount of drosophila chromatin (Active Motif #53083) was added to each sample to allow for quantitative comparisons of samples displaying very different amounts of H3K27 methylation marks (ChIP-Rx ...
-
bioRxiv - Genetics 2024Quote: ... followed by a 1 hour incubation with the appropriate primary antibody (H3K27me1; Active Motif #61015 ...
-
bioRxiv - Genetics 2024Quote: ... followed by a 1 hour incubation with the appropriate primary antibody (H3K27me1; Active Motif #61015, H3K27me3, Millipore #07-449, H3K27M, Active Motif #61803) diluted in Abdil-Tx ...
-
bioRxiv - Cell Biology 2024Quote: ... rabbit anti-H3K9me2 (1:250; AM39041; Active Motif), rabbit anti-Phospho-Rpb1 CTD (Ser2 ...
-
bioRxiv - Cell Biology 2024Quote: Primary antibodies used: mouse anti-H3K27ac (1:250; 1 h; 39085, Active Motif) rabbit anti-H3K27Ac (1:500 ...
-
bioRxiv - Cancer Biology 2024Quote: 10-100nM of recombinant GST-SETD8 protein were incubated with 0.5μg of recombinant mononucleosomes (H3.1, Active Motif #81070) in methyltransferase buffer (30mM Tris (pH 8.0) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and cytoplasmic fractions were extracted using the Nuclear Extract Kit (Active Motif; no. 40010) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... histones from A172 cells treated with scramble and ZNF638 siRNA were purified using a histone extraction kit (Active Motif) as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: Cut&Tag was performed using a Complete CUT&Tag-IT Assay Kit (Active Motif) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... were denatured by heating to 98C for 10min and chilled on ice for 10min and incubated overnight with anti-5hmC antibody (4μl, Active Motif #39791) in 20mM Na-Phosphate pH 7.0 ...
-
bioRxiv - Cancer Biology 2024Quote: ... followed by PCR amplification and barcoding (see Active Motif CUT&Tag kit ...
-
bioRxiv - Biochemistry 2024Quote: ... RNAPII (Active Motif 39497, 1:500), SRSF1 (ThermoFisher 32-4600 ...
-
bioRxiv - Cell Biology 2024Quote: ... 5µl of H3K27me3 antibody (Active motif, Cat#39155) was added to each sample ...
-
bioRxiv - Cell Biology 2024Quote: ... Rabbit polyclonal antibody against H3K27me3 (Cat#39155) was obtained from Active Motif.