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Citations for Active Motif :
1451 - 1500 of 1695 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... rabbit anti-CTCF (Active Motif, 61311, for microChIP-seq), rabbit anti-Rad21 (Santa Cruz ...
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... Antibodies used were rabbit anti-H3K27ac (Active Motif 39133), rabbit anti-H3K4me1 (Abcam ab8895) ...
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bioRxiv - Genomics 2020Quote: ... Antibodies used were rabbit anti-H3K27ac (Active Motif 39133); rabbit anti-H3K4me1 (Abcam ab8895) ...
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bioRxiv - Genomics 2020Quote: ChIP-seq was performed using the ChIP-IT High-Sensitivity kit (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... anti-HP1beta (Active Motif 39979, 1:200); anti-Coilin (Abcam Ab-210785 ...
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bioRxiv - Cancer Biology 2020Quote: SDHD 3’UTR LightSwitch Luciferase Reporter Assay was purchased from Active Motif. Cells were co-transfected with 30 nM miRNA and 100 ng reporter using Lipofectamine 3000 in a 96-well plate ...
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bioRxiv - Genomics 2020Quote: ... and assay was performed with Nuclear Complex Co-IP Kit (Active Motif) as instructed using antibodies against HA-tag (Thermo Fisher cat#26183) ...
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bioRxiv - Developmental Biology 2020Quote: ... following the manufacturer’s instructions and ChIP-grade antibodies for H3K27ac (Active Motif, Cat#39133) or H3K27me3 (Active Motif ...
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bioRxiv - Developmental Biology 2020Quote: ... or H3K27me3 (Active Motif, Cat#39155). The immunoprecipitated samples and input samples were subjected to reversal of cross-linking by adding water to 500 μl and 20 μl 5 M NaCl ...
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The following day the beads were washed once with ChIP Buffer I and twice with ChIP Buffer II (Active Motif) before elution with 50mM Glycine (pH 2.8 ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP Buffer I (Active Motif, La Hulpe, Belgium), 1X PIC and water ...
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bioRxiv - Genomics 2020Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... plus a mouse monoclonal anti-H3K9me3 (1:200; Active motif 39286). For whole-cell proteomics ...
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bioRxiv - Genomics 2020Quote: ... rabbit polyclonal anti-CTCF (1:500; Active motif 61311); rabbit polyclonal anti-H3K27me3 (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... and total H3 (1:1000, Active Motif, #39763).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were probed using antibodies against H4R3me2a (1:1,000, Active Motif, #39705) and total H3 (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with 5-methylcytosine antibody (1:200; Active Motif #39649) at 4 °C overnight ...
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Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5ug antibodies were added to the supernatant and incubated overnight at 4°C with gentle rotation (CTCF, ab70303; RAD21, ab992; H3K4me3, Millipore, 04-745; H3K27ac, Active Motif,39685.). Chromatin was pulled down by protein G Sepharose beads (#17061801 ...
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bioRxiv - Genetics 2020Quote: ... or anti-CTCF (Active motif, 61311). Chromatin-bound antibodies were recovered with 30μl Protein A/G magnetic beads (Millipore 16-663) ...
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bioRxiv - Molecular Biology 2020Quote: ... Harvest and isolation of nuclear and cytoplasmic fractions were performed using Nuclear Extract Kit (Active Motif). Samples were quantified and immunoblotted as described above ...
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bioRxiv - Cell Biology 2020Quote: The following commercially available proteins were used in this study: recombinant SETD2 (a.a. 1418-1714) protein (Active Motif, 31358), rabbit skeletal muscle actin (Cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: ... Histone H3K36me3 (rabbit, Active Motif, 61101), Lamin A/C (rabbit ...
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bioRxiv - Genetics 2020Quote: ... and anti-H3K4me3 (Active Motif 39159). One additional mock sample was done with an anti-GFP antibody (Abcam ab290 ...
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bioRxiv - Genetics 2020Quote: ... or 5μg of anti-H3K27ac (39133, Active Motif). A separate control ChIP was also performed for each sample using 5μg of Rabbit IgG (ab171870 ...
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bioRxiv - Genetics 2020Quote: ... or polyclonal H3K9me3 antibody (AB_2532132, Active Motif). To remove non-specifically bound proteins from the CTCF ChIP ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Developmental Biology 2020Quote: ... or H3K36me3 (Active Motif, 61101) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... a rabbit polyclonal raised against the N-terminal half of recombinant PARP1 (Active Motif Ab 2793257, 1:2000 dilution); anti-C-ter-PARP1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Fragmented DNA was immunoprecipitated with antibodies against mouse H4K20me3 (39672, Active Motif), or negative control mouse IgG (5415 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies used: mouse H4K20me3 (39672; Active Motif), rabbit H4K20me3 (ab9053 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies used: 1:400 mouse H4K20me3 (39672, Active Motif), 1:500 rabbit GAPDH (2118 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were blocked using LI-COR blocking buffer for 1 hour followed by overnight incubation with 1:50 mouse H4K20me3 (39672, Active Motif) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... H3K36me2 (Active Motif, 39255), H3K36me1 (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... anti-EZH2 (Active Motif #39875, 1:10000), anti-H3 (Abcam #Ab1791 ...
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bioRxiv - Neuroscience 2020Quote: ... Systems contained Recombinant PRMT1 (Active Motif, Carlsbad, CA, USA), S-(5-Adenosyl)-L-methionine iodide (SAM ...
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bioRxiv - Neuroscience 2020Quote: ... either Histone H4 (Active Motif) or GR15 (Genic Bio) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-H3K4me3 1:2000 (Active Motif #39159); mouse anti-HA ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 1:200 dilution of PARP2 antibody (Active Motif, 39743) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... PARP2 antibody (Active Motif, 39743, 1:1000 dilution), HA (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10 mM glycerophosphate) with the addition of 40 ng Spike-in Chromatin per reaction (Active Motif 53083). Immunoprecipitation was conducted using 4 µg of anti-H3 (Abcam ab1791) ...
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bioRxiv - Cancer Biology 2020Quote: The DNA-binding activity of NF-κB was quantified by enzyme-linked immunosorbent assay using the TransAM® NF-κB family activation assay kit (Active Motif North America, Carlsbad, CA) to specifically detect and quantify the DNA-binding capacity of the NF-κB subunits p65 ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-RING1B antibody was bound to IgG beads (#39663, Active Motif, 1:500) for 1h at RT before applying 500 μg of the total cell lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... RRID:AB_2716801) for Ptch1+/-;BcorΔE9-10 and Ptch1+/- tumor samples was performed by Active Motif. One sample of Ptch1+/-;BcorΔE9-10 was discarded for outlier levels of Igf2 revealed by RNA-seq after ChIP analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-RING1B (#39663, Active Motif, 1:500), anti-HA (#MMS-101P ...
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bioRxiv - Cancer Biology 2020Quote: ... The coefficients from Drosophila spike-ins were computed based on the alignment statistics and applied to adjust to the reads distribution per sample as suggested by Active Motif. For peak calling Macs 1.4 tool (Zhang et al ...