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Citations for Active Motif :
51 - 100 of 128 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Cells were then processed with the ChIP-IT High Sensitivity kit (Active motif) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed and blocked using the MAXpack Immunostaining Media Kit (Active Motif). Cells were incubated overnight at 4 °C in primary antibody (1:100 Abcam Rabbit anti-Atrx ab97508) ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Cytoplasmic extracts were generated by disrupting cells using hypotonic buffer (Active Motif, Carlsbad, CA) and pelleting the cell debris by centrifugation at 14000 × g for 10 min at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Chromatin immunoprecipitation was performed using the Low Cell ChIP-Seq kit (#53048, Active Motif) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Cells were lysed to ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit (Active Motif 53008). Optimal sonication conditions were determined previously in order to obtain DNA fragments of about 500 bp ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed using 100 µl of ice-cold ATAC lysis buffer (Active Motif), followed immediately by addition of 1 ml pre-chilled wash buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Genomics 2022Quote: ... at a concentration of 10 ng/million cells and spike-in antibody (Active Motif, 61686) at a concentration of 0.4 µg/million cells were added following lysis and sonication but prior to immunoprecipitation ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were then washed in 1x PBS twice followed by histone extraction (Active Motif). The extracted histones were then western blotted for H2B-Ub levels (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: Nuclear extracts from Jurkat cells were prepared using a Nuclear Extract Kit (40010, Active Motif), according to manufacturer’s recommendation ...
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bioRxiv - Genomics 2022Quote: ... 0.5 million G1-phase DLD-1 cells were processed according to manufacturer’s instructions (Active Motif). Samples were paired-end sequenced to obtain at least 107 reads ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were incubated overnight at 4oC with mouse anti-PLZF (0.2 μg/ml, Active Motif) primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... 150k cells were used per sample using the anti-H3K9me3 primary antibody (Active Motif, AB_2532132), the anti-γH2AX primary antibody (Abcam ...
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bioRxiv - Genomics 2023Quote: ... chromatin was extracted from CT26 cells using the Nucleosome Preparation Kit (cat: 53504, Active Motif). The enzymatic digestion time for this cell line was optimized to 30min and the mononucleosome preparation protocol was followed as per manufacturer’s recommendations ...
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bioRxiv - Biophysics 2023Quote: ... melanogaster Schneider cells in presence of 2 µg of anti-His2Av antibody (Active Motif 61686) for each immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 200’000 cells were used per sample using the anti-H3K9me3 primary antibody (Active Motif, AB_2532132). A homemade purified ProtA-Tn5 protein (3XFlag-pA-Tn5-Fl ...
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bioRxiv - Systems Biology 2019Quote: ... Permeabilized cells were incubated overnight at 4°C with 5ug of anti-H3K27me3 (Active Motif 39156) and then washed before incubating with protein A-MNase fusion protein (a gift from S ...
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2021Quote: ... cells were re-suspended in Glycine Stop-Fix Solution (ChIP-IT Enzymatic Express Kit; Active Motif) and kept shaking for 5 mins at room temperature ...
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bioRxiv - Physiology 2019Quote: ... Ishikawa cell nuclear and cytoplasmic protein fractions were extracted using Nuclear Extraction Kit (Active Motif, Belgium) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... were coated with antibodies (Cell Signaling Technology Rabbit anti-H3K27me3 Cat#9733, RRID:AB_2616029 and Active Motif Rabbit anti-H3K4me3 Cat# 39159 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cytosolic extracts from NES cells were prepared by using the Nuclear Extract Kit (Active Motif) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ATACseq was used to evaluate the chromatin of MG63 cells (Active Motif, Carlsbad, CA, USA; 53150). At the Sylvester Comprehensive Cancer Center’s Oncogenomic Core Facility ...
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bioRxiv - Cell Biology 2020Quote: Histones were purified from cells by growing macrophages to a density of 3×107 (Active Motif - 40025). Histone H3K27me3 conversion was measured using the Active Motif kit (Epiquik Histone Methyltransferase activity assay kit (P-3005).
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 4T1 and 6DT1 cells using the ChIP-IT Express Enzymatic kit (Active Motif) according to manufacturers’s instructions as mentioned above ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in 100 µl cold Antibody Buffer and 1 µl antibody (TET1, Active Motif #61943) was added for incubating on nutator overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were washed with 0.1 % PBST three times and blocked in MAXblock Blocking Medium (Active Motif) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... Histones were isolated from inhibitor treated or untreated cells using the histone extraction kit (Active Motif, 40028) and western blots performed ...
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bioRxiv - Genomics 2024Quote: ... and Illumina sequencing libraires were prepared using the Low Cell ChIP-Seq kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionation of MDA-MB-231 and 4T1 cells was first performed using the Nuclear Extract kit (Active Motif). The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif ...
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bioRxiv - Pathology 2020Quote: ... and FAC-sorted β-cells were used for histone H3K27ac ChIPseq with anti-H3K27ac antibody (Active Motif, 39133). ChIP and ChIPseq were performed as previously described (Kuo et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... the rest was incubated with primary antibody (H3K4me3 antibody: Cell signaling Technology, 9727; H3K9me3 antibody: Active Motif, 39161) -coated Dynabeads Protein A/G for overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed cells were washed with chilled PBS-Igepal (0.5% Igepal CA-630 in PBS) and PMSF (Active Motif). Washed cells were centrifuged ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets used for fractionation were fractionated using the Nuclear Complex Co-IP kit from Active motif (#54001) according to their protocol.
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bioRxiv - Developmental Biology 2019Quote: ... 5 µl was transformed into chemically competent TAM-1 cells in a 96-well plate format (Active Motif #11096) and selected for Chloramphenicol resistance ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K4me3 (1:100 for bulk profiling or 1:10 for single-cell experiments, Active Motif Cat# 39159), Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts ...
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bioRxiv - Cancer Biology 2021Quote: Cells from two independent experiments were collected and open chromatin was assessed using an ATACseq kit (Active Motif, 53150). DNAseq library preparation was completed at the Oncogenomics Core Facility at the Sylvester Comprehensive Cancer Center ...
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bioRxiv - Physiology 2020Quote: ... Cells were then washed twice more with ice cold PBS before homoengisation with a Dounce homogenizer (Active Motif, 40415) in Fast IP buffer (150mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... MM.1S cells were exposed to Dex or EtOH for 1 hour and fixed according to the manufacturer’s instructions (RIME Cell Fixation protocol, Active Motif). Analysis were performed by Active motif and resuslts are given as supplementary table (see Additional file 2 ...
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bioRxiv - Cell Biology 2020Quote: Controls and bleomycin-injured MLE12 cells were lysed in ice-cold buffer (PBS, 0.05% Tween 20, pH 7.4) containing protease inhibitors (Active motif, 37491) and phosphatase inhibitor (Active motif ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitations were performed using 30 μg of cell line chromatin and 6 μg of N-Myc antibody (Active Motif # 61185) or 4 μg of H3K27Ac antibody (Active Motif #39133) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S; anti-HDAC2, Active motif, #31505 ...
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bioRxiv - Cell Biology 2020Quote: Fibroblast cells were lysed in ice cold buffer (PBS, 0.05% Tween 20, pH 7.4) containing protease inhibitors (Active motif, 37491) and phosphatase inhibitor (Active motif ...
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bioRxiv - Genetics 2020Quote: ... containing primary antibody (rabbit anti-H3K9me3, Abcam ab8898; rabbit anti-H3K27me3, Cell Signaling Technology C36B11; rabbit anti-H3K4me3 Active Motif 39159 ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...