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Citations for Active Motif :
551 - 600 of 612 citations for Rat Dynamin 1 Like Protein DNM1L ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: One μg of histone H3 peptides with or without H3K4me2 (a.a. 1-21, Active Motif) were incubated with 1 μg of purified GST-PHF21A protein fragments in binding buffer (50 mM Tris/Cl at pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated with primary anti-5mC antibody (1:200; Active Motif, Carlsbad, CA, USA) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... We used a rabbit anti-Cid/CENP-A antibody (Active Motif cat. 39713, 1:50) and rabbit anti-IgG as negative control (1:100) ...
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bioRxiv - Cancer Biology 2021Quote: The effect of CLEFMA on NF-κB p65-DNA interaction was assessed in the nuclear fraction of H441 and CCL151 cells by TransAM NF-κB p65 kit according to the manufacturer’s instructions (Active Motif, Carlsbad, CA, USA). A specific NF-κB inhibitor ...
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bioRxiv - Immunology 2022Quote: ChIP-qPCR analysis were performed using formaldehyde fixed purified BM pDCs with ChIP-IT® Express Chromatin Immunoprecipitation Kits (53035, Active Motif, USA) according to the manufacturer’s instructions ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... DNA binding capacity of the Nf-κB subunits relB and p52 were determined with the Nf-κB TransAM family kit (Active Motif, Rixensart, Belgium) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq)59 was performed from HeLa cells using the ChIP-IT High Sensitivity Kit from Active Motif (reference 53040) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were DNase treated and lysed in ice-cold ATAC seq lysis buffer from the ATAC-Seq Kit (Active Motif, Carlsbad, CA, USA) for 2 min ...
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bioRxiv - Bioengineering 2024Quote: Chromatin immunoprecipitation followed by real-time quantitative PCR (ChIP-qPCR) was performed using the ChIP-IT PBMC kit (53042; Active Motif, Carlsbad CA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was blocked in PBS BSA 1% and incubated with anti-5hmC (39769; Active Motif) or anti-ssDNA antibody (LS-C64821 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoblots were stained overnight with the following primary antibodies: Anti-PARP1 (1:1000 Active motif, 39559), Anti-DNMT1 (1:1000 ...
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CENP-C regulates centromere assembly, asymmetry and epigenetic age in Drosophila germline stem cellsbioRxiv - Cell Biology 2020Quote: ... the following antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:1000), sheep anti-CENP-C (Dattoli et al ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were stained with anti-acetylated histone H3 antibody (1:250; #39140, Active Motif, CA, USA) overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Antibodies were used at the following concentrations: Anti H3K27ac 1:1000 (Active Motif Cat No. 39685), and /or Anti H3K27me3 1:1000 (Active Motif Cat No ...
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bioRxiv - Molecular Biology 2020Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq) (23) was performed from HeLa cells using the ChIP-IT High Sensitivity® kit from Active motif (ref #53040) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Cell Biology 2019Quote: Isolation of nuclear protein extracts from ATI-like cells after 6 days of culture was performed with a Nuclear Extract Kit according to the manufacturer’s instructions (Active Motif, 40010, La Hulpe, Belgium) as described (Hofmann et al. ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Molecular Biology 2023Quote: CUT&Tag was performed as described previously (Kaya-Okur et al., 2020; Kaya-Okur et al., 2019) using the CUT&Tag-IT Assay Kit from Active Motif (Catalog No. 53160) and following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in 100 µl cold Antibody Buffer and 1 µl antibody (TET1, Active Motif #61943) was added for incubating on nutator overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
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bioRxiv - Developmental Biology 2022Quote: ... the following primary antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:500), sheep anti-CENP-C (Dattoli et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Molecular Biology 2019Quote: We performed immunoprecipitations with 5 μg 1°Ab (Target: SATB2, Abcam, positive control: RNA pol II, Active Motif, negative control ...
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bioRxiv - Genomics 2023Quote: ... incubated for 45 min at RT with primary antibody diluted in 1X PBS/1% BSA (H3K27me3, Active motif-61017 ...
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bioRxiv - Cancer Biology 2020Quote: The DNA-binding activity of NF-κB was quantified by enzyme-linked immunosorbent assay using the TransAM® NF-κB family activation assay kit (Active Motif North America, Carlsbad, CA) to specifically detect and quantify the DNA-binding capacity of the NF-κB subunits p65 ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... 1 mg of total HEK293T cellular extract was incubated with 5 μg of anti-SET8 antibody (Active Motif, 61009), anti-LSF antibody (Millipore,17-10252) ...
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bioRxiv - Developmental Biology 2019Quote: ... 5 µl was transformed into chemically competent TAM-1 cells in a 96-well plate format (Active Motif #11096) and selected for Chloramphenicol resistance ...
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bioRxiv - Biochemistry 2020Quote: ... a rabbit polyclonal raised against the N-terminal half of recombinant PARP1 (Active Motif Ab 2793257, 1:2000 dilution); anti-C-ter-PARP1 ...
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bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...
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bioRxiv - Cancer Biology 2023Quote: ... the bead/nuclei mixture was incubated overnight with a 1:100 dilution of primary antibody (H3K27ac (39133, Active Motif)) which was followed by secondary antibody (13-1047 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... MM.1S cells were exposed to Dex or EtOH for 1 hour and fixed according to the manufacturer’s instructions (RIME Cell Fixation protocol, Active Motif). Analysis were performed by Active motif and resuslts are given as supplementary table (see Additional file 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). Immunoprecipitated material and input chromatin were submitted to the Genomics Core Facility (GCF ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). PCR primers were designed in the TSS region for selected genes (Additional file 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin samples were incubated for 5 min at 37°C followed by a second incubation for 10 min at 37°C with shaking at 1,000 rpm after the addition of 1:100 enzymatic working solution (Active Motif, ChIP-IT Enzymatic Kit ...
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bioRxiv - Neuroscience 2022Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S; anti-HDAC2, Active motif, #31505 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Genomics 2021Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041; H3K27Ac, Active Motif #39133 ...
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bioRxiv - Neuroscience 2022Quote: ChIP-seq libraries were constructed from FACS-enriched RGCs (100,000 cells for each library) using an H3K27me3 antibody (1:100, Active Motif) or normal rabbit IgG (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: ... IP sample was incubated with 1 µg H3K4me3 antibody (in-house) (89) and 2 µg H3K27me3 antibody (Active Motif, Cat 61017) overnight with rotation at 4℃ ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated cells were picked in 1 μl of 1× PBS and transferred into 4.4 μl of lysing buffer containing 4 μl mTRAP™ Lysis Buffer (Active Motif) and 0.4 μl (10 ng ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
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bioRxiv - Immunology 2020Quote: ... and then incubated for 2 hours at room temperature with respective primary antibodies (at dilutions of 1:100): H3K27me3 (39155, Active Motif); Ubiquityl-histone H2A Lys119 (8240 ...