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Citations for Active Motif :
501 - 550 of 612 citations for Rat Dynamin 1 Like Protein DNM1L ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif, Cat. 39040), and 1:500 dilution mouse anti-human β-actin (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... and then probed with either 1:10,000 anti-H3K9ac antibody (Active Motif #39038), 1:1,000 anti-H3K56ac antibody (Active Motif #39133) ...
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bioRxiv - Genomics 2023Quote: ... and then incubated with Anti-KAT6A / MOZ antibody (Active Motif, at 1:500) overnight ...
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bioRxiv - Genetics 2023Quote: ... The following primary antibodies were used: ⍺H3 (Active motif 39763, mAb, 1:5000), ⍺H4K20me1 (abcam 9051 ...
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bioRxiv - Developmental Biology 2023Quote: ... a rabbit anti-H4R3me2a antibody (1:500 dilution; 39706; Active Motif, Carlsbad, CA), a rabbit anti-H3R2me2a antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... the monoclonal antibody anti-pSer5 CTD T4 (Active Motif, 61361, 1:200 dilution). The anti-GFP (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear extracts were isolated using the reagents and protocol provided in the Active Motif Nuclear Extract Kit (Active Motif). IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif) ...
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bioRxiv - Developmental Biology 2019Quote: ... All H3K27ac ChIP experiments were processed as ChIP-seqs using the reagents from ChIP-IT High Sensitive kit (Active Motif). IPs were performed in parallel technical duplicates with 11 to 14µg of chromatin on each ...
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bioRxiv - Microbiology 2019Quote: ... Cell fixation was performed using the fixation reagents and steps from the Low Cell Chip Kit (Active Motif; Cat#53084) as described in the user manual ...
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bioRxiv - Microbiology 2019Quote: Immunoprecipitation of both RNA-DNA hybrids (DRIP) and γH2A was performed using the ChIP-IT Enzymatic Express kit (Active Motif) with formaldehyde fixed chromatin samples ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays at the HES1 promoter were performed in HT29 cells using the CHIP-IT kit (Active Motif, Carlsbad, USA). Sonicated chromatin was immunoprecipitated with antibodies against IgG (sc-3739 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies used were: PARP1 (1:1000, 39559, RRID: AB_2793257, Active Motif), Anti-poly-ADP-ribose binding reagent (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used in this paper were rabbit anti-CID (1:1000, Active motif), rabbit anti-SPC105R (1:4000 ...
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bioRxiv - Genomics 2023Quote: ... Antibodies used: mouse anti-Srebp1 (39939, Active Motif, Carlsbad, CA, USA, dilution 1:1,000), rabbit anti-Srebp2 (112046 ...
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bioRxiv - Molecular Biology 2023Quote: Global DNA methylation LINE-1 were measured by Active Motif (55017, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... After quenching with 0.5M glycine the HMGN-nucleosome complexes were immunoprecipitated using ChIP-IT Express kit (Active Motif, Cat. No. 53008) with either anti-HMGN1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified DNA was analyzed by ChIP-qPCR data relative to input using the mouse negative control and mouse positive control primer set Gapdh in the ChIP-IT qPCR analysis kit (Active Motif). Reaction conditions were programmed with initial denaturation at 50°C for 2 minutes and 95°C for 10 minutes followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tagmentation Master Mix was added to the pellet and reaction was incubated in a themomixer at 600-800 rpm set at 37C for 30 min as indicated by manufacturer (ATAC-Seq kit, 53150, Active Motif).
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: The binding of the p65 subunit of NFκB was quantified using the TransAM™ NFκB p65 kit (Active Motif, Carlsbad, CA), as we previously reported (Bai et al ...
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Epigenetic Changes in Saccharomyces cerevisiae Alters the Aromatic Profile in Alcoholic FermentationbioRxiv - Microbiology 2022Quote: The HDAC inhibition capacity of candidate epigenetic modifiers was measured by a fluorometric HDAC assay kit (Active Motif, Inc., CA, USA), following the manufacturer’s instructions with slight modification to suit the objectives of this research ...
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bioRxiv - Cancer Biology 2023Quote: PB NK cells were stimulated or not with IL-12 as previously described and Ch-IP was performed using ChIP-IT PBMCs Kit (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was sonicated (300-700 bp size) and MIRA pull-down was performed using the Methyl Collector Ultra Kit (Active Motif), which uses a MBD2b/MBD3L1 protein complex ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were carried out according to the manufacturer’s instructions with the Gelshift™ Chemiluminescent EMSA Kit (#37341, Active Motif, Waterloo, Belgium). Purified M35_S protein was diluted in storage buffer to reach the indicated concentration (0.1 to 10 µM ...
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bioRxiv - Molecular Biology 2024Quote: ATAC-seq libraries were generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the ATAC-seq kit (Active Motif). H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC ...
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bioRxiv - Neuroscience 2024Quote: Cells from proliferating iMOPs and iMOP-derived neurons were harvested for and processed using the CUT&Tag-IT Assay Kit (Active Motif) described by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs (10 million cells / Co-IP) were collected and prepared for nuclear extraction and purification using a nuclear extraction kit (Active Motif). Nuclear extracts were treated with an enzymatic shearing Cocktail for DNA digestion to release undissociated protein complexes ...
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bioRxiv - Neuroscience 2019Quote: ... hiPSC-derived neurons were processed using the Active Motif ChIP-IT High Sensitivity kit according to the manufacturer’s recommendations (Active Motif, Cat. 53040). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: The ChIP was performed by using ChIP-IT® Express Kits according to the manufacturer’s instructions (Active Motif, San Diego, CA, USA). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Genetics 2022Quote: ... the nuclei suspension was diluted in a 1X volume of 1X Wash Buffer from a CUT&Tag-IT Assay Kit (Active Motif 53160). To estimate the yield of nuclei ...
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bioRxiv - Microbiology 2022Quote: ... parasites were centrifuged 2000xg for 10min and the parasite pellet used directly with the CUT&Tag-IT Assay Kit (Active Motif 53160). Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples in the 300-500bp range were used for immunoprecipitation using the ChIP-IT High Sensitivity kit (Active Motif, cat. no. 53040). DNA was purified using a Zymo DNA Clean & Concentrator-5 kit (Zymo ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Cell Biology 2023Quote: ... Reporters and mimics/inhibitors were reverse transfected with Lipofectamine 3000 (Fisher) and luminescence read after 48 hours with the LightSwitch™ Luciferase Assay Kit (Active Motif).
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bioRxiv - Developmental Biology 2019Quote: ... The primary antibodies used were as follows: H3K27me3 (mouse, 1:500, Active motif, cat. 61017), H3K27ac (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibodies were used in IHC: PARP1 (1:100, 39559, RRID: AB_2793257, Active Motif; 1:100 ...
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bioRxiv - Immunology 2019Quote: ... Then beads were incubated with H3K27ac antibody (1 µg per IP, rabbit, Active Motif, 39133) or normal rabbit IgG (1 µg per IP ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5-1 μg of primary antibody with 0.25 μg Spike-in antibody (Active Motif 61686) diluted in Digitonin block buffer was added to the bead-nuclei mixture and incubated for 3 hours (histone marks ...
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bioRxiv - Genomics 2019Quote: ... transferred to PVDF membranes and probed with primary antibodies to BAF170 (Active Motif, 1:1000) and Histone H4 (1:6000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and H3K9ac antibody (Rep 1-1.4 ug or Rep 2-5 ug) (Active Motif, 39917). Enrichment was calculated relative to the geometric mean of two genes highly enriched for both H3K79me2 and H3K9ac in human (h ...
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bioRxiv - Genomics 2022Quote: ... 0.5 million G1-phase DLD-1 cells were processed according to manufacturer’s instructions (Active Motif). Samples were paired-end sequenced to obtain at least 107 reads ...