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Citations for Vector Labs :
401 - 450 of 599 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Bioengineering 2022Quote: ... Retinas were then washed 3 times in PBS at RT and incubated with polyclonal rabbit LM opsin and S opsin antibody (1/300; Vector Laboratories, Peterborough, UK) for 48 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the slides were incubated with secondary antibodies for 1 hour and washed again 3 times with PBS and mounted with Vectaschield containing DAPI (Vector Laboratories, H-1500).
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with PBS and incubated for at least an hour with blocking buffer containing 3% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Cell Biology 2024Quote: ... and after 3 more washes with PBS the coverslips were mounted with Vectashield solution (mounting medium with DAPI, Vector Laboratories Inc., Burlingame, CA). Evaluation was done using a Nikon 80i or Bio-rad confocal microscope ...
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bioRxiv - Molecular Biology 2021Quote: ... each for 5 min using a 42°C hot plate) before being mounted with VECTASHIELD with DAPI (Vector Labs) onto Superfrost Plus microscopy slides (VWR) ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were blocked with DPBS containing 5% (w/v) normal goat serum (Vector Laboratories, Burlingame, CA, United States), 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... sectioned (5 um) and processed for immunohistochemical analysis post heat-mediated antigen retrieval at pH 6.0 (Vector Labs, USA) except sections stained with anti-SOD1 antisera that were subject to antigen retrieval at pH 9.0 (Vector Labs ...
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bioRxiv - Biophysics 2020Quote: ... 1.6 kb) or control nontelomeric DNA (noTel, 4.1 kb) using the 5’ EndTag Labeling DNA/RNA Kit (Vector Laboratories). The biotinylation of the pT270 and noTel fragments was verified using AFM imaging of DNA samples in the presence of streptavidin-coated quantum dots (strep-QDs ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were incubated overnight with Dolichos biflorus lectin conjugated to Tetramethylrhodamine-5-(and 6)-isothiocyanate (DBA-TRITC, Vector Lab) at 10 μg/mL diluted in blocking buffer at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Peanut Agglutinin conjugated with rhodamine was used at 1:1000 to identify cones (Vector Laboratories, catalog # RL-1072-5). Secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... coverslips were washed 5 times with 1×PBS and mounted with VECTASHIELD antifade mounting medium (Vector Laboratories, CA, USA). Nuclear intensity of at least 15 infected cells was measured for each experiment and coverslip.
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bioRxiv - Neuroscience 2021Quote: ... and 1000 mM) or 500 mM KCl in 0.1 Trizma buffer with 5 - 10% neurobiotin (Vector Labs, Birmingame, USA). They were then beveled from an initial resistance of 40 - 150 MΩ to 30 - 40 MΩ using a rotary beveller (Sutter BV-10) ...
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bioRxiv - Microbiology 2021Quote: ... Cell nuclei were labeled with Hoechst 1:10,000 for 5 minutes and coverslips were mounted using Vectashield (Vector Laboratories). Slides were imaged using a Leica DM6000 Widefield microscope.
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bioRxiv - Cell Biology 2021Quote: ... washed three times with PBS at RT for 5 min and finally mounted with VECTASHIELD mounting medium (Vector Laboratories). Primary antibodies were rabbit antibodies against DDX6 (Bethyl ...
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bioRxiv - Cell Biology 2023Quote: ... After washing for 5 min three times the samples were mounted with antifade mounting medium (Vector Laboratories, H-1700). The samples were imaged on Leica TCS SP8 STED 3X CW 3D microscope using HC PL APO 93x/1.30 motCORR STED WHITE (glycerol ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 μm sections were de-paraffinized and treated with citric acid-based Antigen Unmasking Solution (Vector Laboratories, #H-3300) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and washed two times for 5 min in MQ before mounting with Vectashield mounting media (Vector Laboratories, #H-1000). Lipid content was quantified using Qupath version 0.2.2 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were washed three times with PBS and then blocked with normal horse serum (NHS; 5%; Vector Laboratories) for 1h at RT or overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... 5 % of Potter-dissociated whole brain homogenate was labelled with rhodamine-conjugated Dolichos Biflorus Agglutinin (Vector Laboratories RL-1032). Cysts were counted using an inverted fluorescence microscope with a 20X objective ...
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bioRxiv - Genomics 2023Quote: ... each for 5 min using a 42°C hot plate) before being mounted with VECTASHIELD plus DAPI (Vector Labs) onto Superfrost Plus microscopy slides (VWR) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µg/ml) and rhodamine-conjugated Ulex Europaeus Agglutinin I (UEA I) (RL-1062, Vector Laboratories, 10 µg/ml) was applied on the slides ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sections were then washed for 5 minutes in PBS and stained with DAB Peroxidase Substrate Solution (Vector Labs), for 3 minutes under the microscope ...
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bioRxiv - Genetics 2024Quote: ... The chromosomes were counterstained with 5 μg/mL of 4,6-diamidino-2-phenylindole (DAPI) in Vectashield (Vector Laboratories, USA) then observed under a BX43 phase-contrast microscope or a BX-53 fluorescence microscope (both from Olympus ...
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bioRxiv - Genetics 2024Quote: ... 6-diamidine-2-phenylindole dihydrochloride in 2XSSC) for 5 min and then mounted in Vectashield H-1000 (Vector Laboratories). Samples were imaged using a fluorescent Nikon microscope equipped with a cooled CoolSnap CCD camera (Photometrics) ...
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bioRxiv - Plant Biology 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) was diluted to 5 μg/mL in Vectashield antifade mounting media (Vector Laboratories).
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for monoclonal antibodies; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for polyclonal antisera and monoclonal antibodies, respectively; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then washed 3 times and treated with avidin-biotin complex solution (VECTASTAIN® Elite ABC-HRP Kit, Vector Lab, #PK-6100) for 90 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Following one (cell lines) or 3 days expression (primary hippocampal cultures) cells were fixed using paraformaldehyde and coverslips mounted using Vectashield (Vector Laboratories, Burlingame, CA, USA) with or without DAPI (cell lines) ...
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bioRxiv - Neuroscience 2023Quote: ... then reacted for visualization of peroxidase with nickel-cobalt intensified diaminobenzidine for 3 - 8 minutes (DAB Peroxidase substrate kit SK-4100, Vector Laboratories, Burlingame, CA, USA). Brain slices were washed between each incubation step using TBS (3 × 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... cells were washed 3 times with PBS on ice and bound biotinyl tyramide was visualized with fluorescent streptavidin (Vector Laboratories, Inc., Burlingame, CA, USA) at 1:1000 in PBS for 30 minutes at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times for 5 min in PBST and mounted in Vectashield mounting medium with DAPI (H-1200; Vector Laboratories). Primary antibodies used for immunofluorescence were ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 min in PBT and mounted in VectaShield medium (Vector Laboratories, cat#H-1000). Images were acquired on a Zeiss LSM 710 confocal microscope using a 25X immersion objective (Zeiss ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were blocked with 5% donkey serum (and Mouse-on-Mouse block as necessary from Vector Laboratories, Inc., Burlingame, CA) and incubated overnight at 4°C with an antibody against either Perlecan (1:50 ...
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bioRxiv - Immunology 2021Quote: ... The eOD-60mer was affinity purified by incubating with Galanthus Nivalis Lectin-conjugated agarose beads (AL-1243-5, Vector Laboratories) overnight under gentle agitation at 4°C and eluted with lectin elution buffer containing 1 M Methyl a-D-mannopyranoside (M6882 ...
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bioRxiv - Physiology 2020Quote: ... the sections were stained using the avidin-biotinylated-peroxidase ABC kit followed by a 5-min treatment with 3,3’-diaminobenzidine (DAB, SK-4100, Vector Laboratories). Stained sections were observed and imaged on a Nikon E-1000 microscope (Japan ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in 1x PBS three times for 5 min and mounted with Vectashield antifade mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed three times in PBT for 5 minutes then incubated in biotinylated anti-rabbit IgG (Vectastain, Vector Laboratories) at a concentration of 1:200 in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Immunology 2022Quote: ... washed in PBS+0.1% Tween 20 for 5 min three times and mounted with mounting media containing DAPI (Vector Laboratories). Fluorescent images were captured using BZ-X810 Inverted Microscope (Keyence).