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Citations for Vector Labs :
451 - 500 of 599 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in 1x PBS three times for 5 min and mounted with Vectashield antifade mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed three times in PBT for 5 minutes then incubated in biotinylated anti-rabbit IgG (Vectastain, Vector Laboratories) at a concentration of 1:200 in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Immunology 2022Quote: ... washed in PBS+0.1% Tween 20 for 5 min three times and mounted with mounting media containing DAPI (Vector Laboratories). Fluorescent images were captured using BZ-X810 Inverted Microscope (Keyence).
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bioRxiv - Immunology 2022Quote: ... The cells were washed and stained for 30 min at 4°C with 5 μg/ml FITC- WGA (Vector Laboratories). After the wash ...
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bioRxiv - Molecular Biology 2020Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... The sections were then washed with PBS three times for 5 min and incubated with avidin-biotin complex reagent at a dilution based on the manufacturer’s recommendations (Vector Laboratories) for 30 min at RT ...
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bioRxiv - Physiology 2020Quote: ... incubated in 4’6’-diamidino-2-phenylindole (DAPI) (1:1000) in TBS for 5 minutes then mounted in Vectashield (Vector Laboratories). Fluorescent images were captured using a Nikon A1R-s confocal laser scanning microscope at the BCM Integrated Microscopy Core and processed with the Nikon Perfect Focus System (Nikon Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Genetics 2021Quote: ... the sections were washed 3 times in PBS for 5 minutes each and mounted with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories) and sealed with nail polish ...
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bioRxiv - Immunology 2021Quote: ... Tissue sections were fixed with ice cold acetone for 5 min and blocked with Avidin/Biotin Blocking Kit (Vector laboratories) and Protein block (Protein block (Dako ...
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bioRxiv - Neuroscience 2022Quote: ... endogenous peroxidase activity was blocked with 0.3 % H2O2 for 10 min and sections were blocked with 5 % normal goat serum (Vector Labs) in PBS for 1 h at RT ...
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bioRxiv - Physiology 2022Quote: ... EDL and TA sections were stained with 1:1000 fluorescein wheat germ agglutinin (WGA; 5 μg/mL; Vector Laboratories Ltd.) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in secondary antibody in 5% NGS for at least 2 overnights and mounted using Vectashield (Vector Laboratories H-1000). After each step ...
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bioRxiv - Neuroscience 2024Quote: ... After an hour of cool-down slides were washed with PBS and incubated with blocking buffer (1% BSA, 5% FBS, 0.01 % Triton-X 100 in 1x PBS) followed by Avidin/Biotin blocking (Vector laboratories). Slides were incubated overnight at 4°C with primary antibodies (see Supplementary Table 4 ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed in TBS and incubated for 5 minutes in 3,3′-Diaminobenzidine (DAB) (SK-4105, Vector laboratories, CA, USA). Slides were rinsed in water ...
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bioRxiv - Plant Biology 2023Quote: ... slides were washed 3 x 5 min in 1 x PBS and mounted in Vectashield mounting medium with 1.5 μg/mL DAPI (Vector Laboratories). Antibodies and dilutions used in this study are reported in Supplementary Table 3 ...
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bioRxiv - Genetics 2023Quote: ... cells were washed with PBS three times for 5 minutes and mounted with Vectorshield mounting medium with DAPI (Vector Laboratories). Images were captured by the Leica fluorescence microscope ...
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bioRxiv - Immunology 2023Quote: ... and then run slowly over a column with agarose-bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Excess DAPI was washed off with 0.1% Triton X-100/PBS for 5 min and samples mounted with VECTASHIELD (Vector Labs). Images were acquired using Leica TCS SP8 Confocal Microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% normal goat serum followed by an Avidin/Biotin blocking kit (Vector Labs, Burlingame, CA, USA) and then incubated overnight at room temperature with the primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated with 2 µg/mL biotinylated lectins from Vector Labs (VVA B-1235-2, PNA B-1075-5) in conditions as previously described ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol before samples were stained with DAPI (1 µg/ml, 5 min) and mounted in Vectashield (Vector Laboratories). Images were collected with a Leica SP5 II confocal microscope using LAS-AF software (v2.7.3.9723) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sections were washed in 1XPBS for 5 minutes and incubated for 20 minutes with prediluted normal blocking serum (Vector Labs). The sections were incubated for 30 minutes with 60μl Yap/Taz primary antibody (8418 ...
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bioRxiv - Neuroscience 2024Quote: ... A final wash in PBS (for 5 minutes x2) was completed and then 3,3-diaminobenzidine tetrahydrochloride (DAB) (Vector Laboratories, UK) was applied to help visualize the antibody (left for 3 minutes) ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:1000 in 5% nonfat dry milk/TBS-T or goat anti-rabbit IgG-HRP (Vector Laboratories #PI-1000) diluted 1:20,000 in 5% nonfat dry milk/TBS-T – for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed three times in PBS for 5 min and mounted using Vectashield® medium (Vector Laboratories, Burlingame, CA) and coverslips ...
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bioRxiv - Cancer Biology 2024Quote: ... This was followed by incubation for 5 minutes with a quencher (Vector TrueView Autofluorescence Quenching Kit #SP-8400, Vector Labs) to decrease autofluorescence ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% TritonX-100 in PBS) for 30 min at RT followed by another blocking using M.O.M kit (Vector Laboratories BMK-2202). Primary antibodies (mouse anti-MYH3 ...
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bioRxiv - Cell Biology 2020Quote: ... The organoids were centrifuged at 500 x g for 5 min and resuspended in 30 μL Vectashield (Vector Labs; H-1000) using a cut pipet tip and pipetted onto a coverslide ...
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bioRxiv - Genetics 2021Quote: ... slides were washed 3 times in PBS-T for 5 min each and incubated with the biotinylated horse anti-mouse IgG antibody (AK-5002, Vector laboratories) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... and treated with or without PNGase F was assayed by lectin blotting using Concanavalin A (Con A) (Cat. B-1005-5, Vector Laboratories). A total of 10 μg protein each were electrophoresed in a 12% SDS-PAGE gel and transferred onto PVDF membranes at a constant of 300 mA for 2 h ...
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bioRxiv - Genetics 2021Quote: ... then blocked with 5% (v/v) horse serum (ImmPRESS HRP Anti-Rabbit IgG [Peroxidase] Polymer Detection Kit; Vector labs; MP-7401). Sections were incubated with rabbit anti-TCFL5 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNMT3A and DNMT3B was visualized in cross-section (5 µm) of paraffin-embedded tissues using specific primary antibodies and a Vectastain Elite ABC Kit (Vector Laboratories) or AlexaFluor 488-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: Sections were briefly dried then washed 3x in 0.1% Triton X-100 in phosphate buffered saline (PBST) before they were blocked with 5% neutral goat serum (Abcam or Vector Labs). Sections were then incubated for double-labeling experiments with primary antibody (AB ...
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bioRxiv - Neuroscience 2021Quote: ... Processed spinal cord slides were incubated with DAPI (made up in water) for 5 minutes in a hydration chamber at room temperature and a coverslip was mounted with vectoshield (Vector labs). Process slides were imaged on a Leica SP8 confocal microscope.
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bioRxiv - Cell Biology 2021Quote: ... Tween 20 (TBST) for 5 min each and incubated at room temperature for an hour in secondary antibody-HRP (Vector Laboratories). Antibodies were then visualized by peroxidase substrate kit DAB (Vector Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... three times for 5-min and then blocked for 30-min with 30% normal goat serum (Vector Labs, cat# S-1000) and 1X PBS/Triton 0.3% ...
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bioRxiv - Synthetic Biology 2021Quote: ... slides were again washed 5 times for 10 min with 1 × PBS at room temperature and mounted in Vectashield (Vector Laboratories) with 1 µg/ml DAPI ...
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bioRxiv - Immunology 2020Quote: ... tissues were washed twice for 5 minutes in wash buffer and stained using the ImmPRESS universal (Anti-Mouse/Anti-Rabbit) kit (Vector labs).
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bioRxiv - Cell Biology 2021Quote: ... cells were washed twice for 30 min with 10% formamide in 2X SSC before washing for 5 min with PBS and mounting of the coverslips using Vectashield (Vector Laboratories).
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bioRxiv - Immunology 2021Quote: ... slides were washed 5 times with 1X PBS prior to being mounted with VectaShield medium containing DAPI (Vector lab, Burlingame, CA).
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were washed three times in PBTx for 15 min followed by five 5-minute washes in PBS and mounted in Vectashield antifade mounting medium containing DAPI (Vector Laboratories). Samples were imaged on an Olympus FV3000 Confocal Laser Scanning Microscope and processed in Imaris.
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 6 separate times in TBS-T for 5 min and then incubated with HRP-conjugated streptavidin (Vector Laboratories) for an additional hour ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the fixed specimens were washed thrice for 5 min in 1x phosphate buffered saline (1xPBS; pH 7.4) and mounted using Vectashield mounting medium (H-1000, Vector Laboratories, CA). FITC-albumin and 10 kD dextran specimens were imaged using a ZEISS LSM900 confocal microscope with ZEISS Zen acquisition software (blue edition ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 0.5 μg/mL DAPI in PBS for 5 min prior to drying with ethanol and were mounted using Vectashield mounting medium without DAPI (Vector Laboratories). Images were acquired and analysed using a Leica fluorescence microscope ...