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Citations for Vector Labs :
301 - 350 of 1985 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Physiology 2020Quote: ... incubated in 4’6’-diamidino-2-phenylindole (DAPI) (1:1000) in TBS for 5 minutes then mounted in Vectashield (Vector Laboratories). Fluorescent images were captured using a Nikon A1R-s confocal laser scanning microscope at the BCM Integrated Microscopy Core and processed with the Nikon Perfect Focus System (Nikon Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Physiology 2022Quote: ... EDL and TA sections were stained with 1:1000 fluorescein wheat germ agglutinin (WGA; 5 μg/mL; Vector Laboratories Ltd.) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... After an hour of cool-down slides were washed with PBS and incubated with blocking buffer (1% BSA, 5% FBS, 0.01 % Triton-X 100 in 1x PBS) followed by Avidin/Biotin blocking (Vector laboratories). Slides were incubated overnight at 4°C with primary antibodies (see Supplementary Table 4 ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Plant Biology 2023Quote: ... slides were washed 3 x 5 min in 1 x PBS and mounted in Vectashield mounting medium with 1.5 μg/mL DAPI (Vector Laboratories). Antibodies and dilutions used in this study are reported in Supplementary Table 3 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol before samples were stained with DAPI (1 µg/ml, 5 min) and mounted in Vectashield (Vector Laboratories). Images were collected with a Leica SP5 II confocal microscope using LAS-AF software (v2.7.3.9723) ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:1000 in 5% nonfat dry milk/TBS-T or goat anti-rabbit IgG-HRP (Vector Laboratories #PI-1000) diluted 1:20,000 in 5% nonfat dry milk/TBS-T – for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 5 ml of lectin beads (Galanthus nivalis lectin (GNL)-agarose beads (Vector Labs: AL-1243-5) were added to the filtered transfected supernatant and rocked overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Slides were boiled in citric acid-based antigen retrieval solution (H-3300, Vector Labs) for 20 minutes.
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bioRxiv - Neuroscience 2020Quote: ... 5% normal goat serum (Vector Laboratories), 0.2%/1% triton-x (VWR ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were labelled for 5 min with 5 μg/mL 4’6-diamidino-2-phenylindole (DAPI, Vector Laboratories), and mounted using Mowiol (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2021Quote: ... slides were again washed 5 times for 10 min with 1 × PBS at room temperature and mounted in Vectashield (Vector Laboratories) with 1 µg/ml DAPI ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea) simplicifolia lectin 1 (5 μg/ml) (Vector Laboratories; L-1100). OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Microwave antigen retrieval in citric acid-based antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA) was then performed ...
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bioRxiv - Synthetic Biology 2024Quote: ... heat-induced epitope retrieval was performed using citric acid-based antigen unmasking solution (Vector Laboratories) at 110°C for 15 minutes in a programmable antigen retrieval chamber (ARC ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated MPL (5 μg/mL, Vector Laboratories) was then added to the membrane in blocking buffer (1 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... using the 5′ EndTag kit (Vector Labs) as directed by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... using the 5′ EndTag kit (Vector Labs) as directed by the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were permeabilized with 0.5% Triton X-100 for 30 min at room temperature and blocked in 5% goat serum (Vector Laboratories, 1:200), goat anti-rat 488 and goat anti-mouse 647 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories,UK) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories, UK) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... we removed the supernatant and added 1 ml ACSF with DAPI (Vector Laboratories, Cat No.: H-1200-10ml, 5 μ g/ml). Collected single-cell suspensions were used for library construction.
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% goat serum/1% bovine serum albumin/PBS and Mouse on Mouse blocking reagent (Vector Laboratories, Newark, CA, USA), the samples were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Cardiac sections were then washed in PBST and labeled with FITC conjugated Wheat-Germ Agglutinin (1:500; Vector Labs #FL-1021-5) and Goat anti-Mouse IgM Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Another PBS (5 x 5) wash was conducted before the tissue was incubated in Avidin-Biotin Complex (Vector Laboratories, Newark ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two hours with a biotinylated horse anti-mouse antibody (1:500 in PBS containing 0.5% Triton X-100, Vector Laboratories, #BA-2001-.5), followed by three PBS washes ...
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bioRxiv - Neuroscience 2020Quote: ... washed with PBS for 5×5 min and slides were mounted with VECTASHIELD mounting medium with DAPI (Vector Laboratories, USA). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2021Quote: ... and 5% Normal Goat Serum (NGS, Vector Laboratories) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... 5% Goat Serum (Vector Laboratories S-1000-20), 0.5% Tween-20 (Sigma ...
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bioRxiv - Immunology 2024Quote: ... 5% goat serum (S-1000-20 Vector Laboratories), 3% mouse serum ...
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bioRxiv - Cancer Biology 2021Quote: ... antigen unmasking was performed on rehydrated tissue sections using either a citric-acid retrieval buffer (Vector Labs) or Tris-EDTA retrieval buffer (10 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... rehydrated and subjected to antigen retrieval using a pre-heated (5 minutes at 100% microwave power) 1/1000 dilution of citrate-based antigen unmasking solution (Vector Labs, H-3300-250) for 20 minutes in the microwave at 10% power ...
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bioRxiv - Biophysics 2020Quote: ... Purified RNA transcripts were labeled with fluorescein at their 5’ termini using the 5’ EndTag DNA/RNA Labeling Kit from Vector Laboratories following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... The sections were then washed (5 x 5 min, 20 rpm) and processed with avidin-biotinylated horseradish peroxidase complex (Vectastain Elite Kit, Vector Laboratories) in 0.3% PBST for 1 h at room temperature (2 rpm) ...
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bioRxiv - Cancer Biology 2020Quote: ... unspecific bindings (5% BSA, 5% NGS in PBST, 1h) and Avidin/Biotin (Avidin/Biotin Blocking Kit, Vector Laboratories, SP-2001, 15 min). Samples were incubated overnight at 4°C with anti-MDA antibody (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then permeabilized using PBS with 0.1% Triton X-100 for 5 min and blocked in a 5% v /v goat serum (GS, Normal Goat Serum, S-1000, Vector Laboratories IVD) in PBS for 1 h at RT and incubated overnight at 4 °C with primary antibodies (aniP2RY1 ...
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bioRxiv - Pathology 2022Quote: ... permeabilized in PBS with 0.1% Triton X-100 for 5 min and blocked in a 5% v/v goat serum (GS, Normal Goat Serum, S-1000, Vector Laboratories IVD) in PBS for 1 h at RT ...