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Citations for Vector Labs :
251 - 300 of 1985 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2020Quote: Sections were again washed 3×15 min with PBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) (2-hours ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with PBS and incubated for at least an hour with blocking buffer containing 3% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Cell Biology 2024Quote: ... and after 3 more washes with PBS the coverslips were mounted with Vectashield solution (mounting medium with DAPI, Vector Laboratories Inc., Burlingame, CA). Evaluation was done using a Nikon 80i or Bio-rad confocal microscope ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then rinsed with PBS (5 × 5 min) before placement in Vectastain ABC Solution (1:1000; Vector Laboratories) for 1 hour prior to rinsing (5 × 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1 h at RT and then stained with EC specific biotinylated isolectin B4 (Vector lab, B-1205-.5, 1:100 dilution) overnight at 4 °C and followed by staining with FITC- streptavidin (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 21] with 1/100 IB4-DyLight649 (DL-1208-5, Vector Laboratories). Then ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for monoclonal antibodies; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for polyclonal antisera and monoclonal antibodies, respectively; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then washed 3 times and treated with avidin-biotin complex solution (VECTASTAIN® Elite ABC-HRP Kit, Vector Lab, #PK-6100) for 90 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Following one (cell lines) or 3 days expression (primary hippocampal cultures) cells were fixed using paraformaldehyde and coverslips mounted using Vectashield (Vector Laboratories, Burlingame, CA, USA) with or without DAPI (cell lines) ...
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bioRxiv - Neuroscience 2023Quote: ... then reacted for visualization of peroxidase with nickel-cobalt intensified diaminobenzidine for 3 - 8 minutes (DAB Peroxidase substrate kit SK-4100, Vector Laboratories, Burlingame, CA, USA). Brain slices were washed between each incubation step using TBS (3 × 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... cells were washed 3 times with PBS on ice and bound biotinyl tyramide was visualized with fluorescent streptavidin (Vector Laboratories, Inc., Burlingame, CA, USA) at 1:1000 in PBS for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Antigen retrieval in pre-heated citric acid-based buffer (1:100 in water, Vector Laboratories, Cat#H-3300) was performed in a pressure cooker (Instant Pot ...
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bioRxiv - Neuroscience 2024Quote: ... 3 x 10min PBS washes were followed by mounting in VECTASHIELD® HardSet™ mounting medium with DAPI (Vector Labs, UK, Cat# H-1500-10).
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bioRxiv - Neuroscience 2024Quote: ... Area X (n = 4) and (ii) HVC (n = 3; [14]. The sections were rinsed in PBS, subjected to antigen retrieval (Vector Laboratories, H-3300, pH 6) and treated with 2N HCl ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS 3 more times (10 min, room temperature) and mounted to microscope slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, cat. # H-1200-10). Sections were imaged on a Nikon A1R confocal microscope at the Tufts University Center for Neuroscience Research Microscopy Core ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in antigen retrieval (1:100, antigen unmasking solution, citric acid based, catalog number: H-3300, Vector Laboratories) solution at 92°C for 10 mins ...
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bioRxiv - Immunology 2020Quote: ... and nonspecific epitopes (species-specific serum diluted 1:5 in Tris-buffered saline and 5% bovine serum albumin, or blocking serum from species specific ImmPRESS® kit; Vector Laboratories) and incubated with primary antibody (Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... together with Fluorescein Avidin DCS (1 μl/ml, Vector Laboratories A-2001-5,) in PBS and 0.3% triton X-100 overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Sialic acid expression was determined using biotinylated SNA (α-2,6 sialic acid) and MALII (α-2,6 sialic acid) lectins purchased from Vector Laboratories. 1 × 105 6419c5 cells were incubated with 2 µg/mL of biotinylated lectin for 45 minutes at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Muscle sections were incubated in 1:1000 Wheat germ agglutinin (WGA– fluorescein 5 μg/mL; Cat# FL-1021-5, Vector Laboratories Ltd., Peterborough, UK) for 10 min followed by three washes of PBS-T for 5 min each ...
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bioRxiv - Genetics 2021Quote: ... 4’6’-diamidino-2-phenylindole (DAPI) diluted 1:5 in Vectashield mounting media (Vector Labs) was used as a counterstain ...
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bioRxiv - Neuroscience 2023Quote: ... Blocking for 1 h with 5% (vol/vol) normal goat serum (NGS, Vector Laboratories) was followed by incubation with the appropriate primary antibody at 4°C for 48 h in 2% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... Blocking for 1 h with 5% (vol/vol) normal goat serum (NGS, Vector Laboratories) was followed by incubation with the appropriate primary antibody at 4°C for 48 h in 2% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation with the biotinylated secondary antibody (1:150, Vector in 5% NGS in PBST) for 1 h was followed by the Vector ABC Kit (Vector Laboratories, solution A and solution B in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... diluted 1:1000 in the presence of 5% normal horse serum (Vector Laboratories inc, Burlingame, CA), Next day ...
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bioRxiv - Genomics 2024Quote: ... then counterstained as above with a 1:5 dilution of DAPI in Vectashield (Vector Laboratories, Inc.). As above ...
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bioRxiv - Neuroscience 2024Quote: ... and preblocked for 1 h at 4°C in 5% normal goat serum (NGS; Vector Laboratories), 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS (5 x 5 min) followed by a 1 hour incubation in biotinylated goat anti-rabbit secondary (1:500 in PBS, Vector Laboratories, BA-1000). Sections were rinsed in PBS (5 x 5 min ...
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bioRxiv - Physiology 2023Quote: ... samples were immersed in boiling citric acid buffer (Vector Labs, H3300) for 15-30 minutes and allowed to cool to room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Antigen retrieval was performed with 0.1 mM citric acid (Vector Labs) for 20 min in the microwave at 10% power and then cooled to room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... followed by citric-acid-based antigen retrieval (Vector Laboratories# H-3300) for 30 min in a steam cooker ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were transferred to citric acid-based antigen unmasking solution (Vector Laboratories) and heated in a 2100 antigen-retriever (Aptum Biologics) ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking for 1 h with 5% (vol/vol) normal goat or rabbit serum (NGS or NRS) (Vector Laboratories, #S-1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... A 5′ Cy5 fluorescent label was incorporated using the 5′ EndTag kit (Vector Labs) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 5) ABC kit (Vector Labs); and 6 ...
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bioRxiv - Cell Biology 2024Quote: ... three PBS washes were performed for 5 min each and the slides were incubated for 1 hour with the secondary antibody (Vector Lab, BA-1000, 1:200 dilution), followed by three PBS washes for 5 min each ...
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bioRxiv - Neuroscience 2021Quote: ... Peanut Agglutinin conjugated with rhodamine was used at 1:1000 to identify cones (Vector Laboratories, catalog # RL-1072-5). Secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... coverslips were washed 5 times with 1×PBS and mounted with VECTASHIELD antifade mounting medium (Vector Laboratories, CA, USA). Nuclear intensity of at least 15 infected cells was measured for each experiment and coverslip.
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bioRxiv - Microbiology 2021Quote: ... Cell nuclei were labeled with Hoechst 1:10,000 for 5 minutes and coverslips were mounted using Vectashield (Vector Laboratories). Slides were imaged using a Leica DM6000 Widefield microscope.
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bioRxiv - Immunology 2023Quote: ... antigen retrieval was performed using citric acid–based antigen unmasking solution (Vector Laboratories) on 5 μm sections from FFPE Swiss rolls of distal small intestine ...
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bioRxiv - Neuroscience 2020Quote: ... The next day the slides were rinsed 3 times for 5 minutes in 1X PBS and then incubated for 1 hour at RT with anti-rabbit biotinylated secondary antibody (Vector Laboratories, product no: BA-1000 (1:500)) diluted in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...