Labshake search
Citations for Vector Labs :
251 - 300 of 381 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Sections were again washed 3 times (15 minutes) in TBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories) for 2 hours at room temperature before additional washes in TBS (2 times ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were then washed with TBST (3 ×15 min) and mounted on glass slides with VECTASHIELD Vibrance antifade mounting medium with DAPI (Cat# 1800, Vector laboratories).
-
bioRxiv - Microbiology 2024Quote: ... Preparations were then washed 3 times with PBS to remove excess secondary antibody and the slides were mounted in Vectashield (#H-1000, Vector Laboratories). Images were obtained using a LSM980 Airyscan 2 microscope (Zeiss ...
-
bioRxiv - Neuroscience 2024Quote: ... Transverse spinal cord and brain sections (30 μm) were incubated in blocking solution (3% normal goat serum [#S-1000; Vector Laboratories] or normal donkey serum [#017-000-121 ...
-
bioRxiv - Cancer Biology 2024Quote: Frozen sections were thawed to room temperature and equilibrated for 3 min in PBS before being circled by an ImmEdge hydrophobic pen (Vector Laboratories). Sections were blocked for 45 minutes in 5% BSA in PBS and then incubated with primary antibodies in 5% BSA overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... After 3 washes in PBS cells were incubated with a blocking medium consisting of (5% (w/v) bovine serum albumin (Vector Laboratories) and 0.2% (v/v ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200, BA-1000, Vector Laboratories). Color reaction was performed using the Vectastain Elite ABC Kit (PK-6100 ...
-
bioRxiv - Genetics 2020Quote: ... Sections were incubated with primary antibody (supplementary table S4) overnight at 4°C and with universal biotinylated horse anti-mouse/rabbit immunoglobulin G secondary antibody (Vector Laboratories) for 1 hour ...
-
bioRxiv - Physiology 2021Quote: ... Ears were then gently shaken (120 rpm) at 4°C overnight in 20 μg/ml Dylight 488 strepavidin (SA-5488, Vector Laboratories) and 0.05 mg/ml DAPI (D9542 ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS + 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were mouse anti-abd-A (gift of Ian Duncan) ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
-
bioRxiv - Microbiology 2021Quote: ... were applied for 1 h at 37°C prior to mounting onto slides using mounting media containing DAPI (Vectashield, Vector Labs). Images were obtained on a Leica SP5 confocal microscope or a Nexcelom Celigo as described in individual figure legends.
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
-
bioRxiv - Developmental Biology 2022Quote: ... In case antigen retrieval was needed slides were heated to 90°C in a Tris-based buffer (Vector Laboratories, H-3301) The slides were allowed to cool for at least 30 min and washed in 1xPBST before incubation with primary antibodies The respective primary antibodies were diluted in 1% NGS overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... dried sections were stained overnight at 37°C in an X-Gal staining solution and subsequently counterstained with Nuclear Fast Red (Vector Laboratories). Nissl staining was performed by incubating sections in a 1% thionin solution for 60 min at RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... at 98°C for 15 min for antigen retrieval and incubated in blocking buffer or the MOM Basic kit (Vector Labs) and stained with antibodies as previously described.
-
bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... Slides were blocked with 10% normal goat or horse serum followed by overnight incubation with primary antibody at 4°C and 30 min incubation with ImmPRESS HRP-conjugated secondary antibodies (Vector Laboratories) at room temperature ...
-
bioRxiv - Developmental Biology 2021Quote: ... samples were washed three times in wash buffer for 20 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs). Images were acquired using an upright Leica TCS SP8 confocal microscope with a 63X oil immersion objective lens (NA = 1.4 ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with primary antibodies at 4°C overnight followed with biotinylated HRP-conjugated secondary antibody (Vector Laboratories # BA-1000) at room temperature for 1 hour ...
-
bioRxiv - Cancer Biology 2022Quote: ... The IHC staining of c-Jun and ki-67 was performed using a VECTASTAIN Universal Elite ABC Kit (Vector Laboratories, USA). The sections were stained with a DAB Substrate Kit (Vector Laboratories ...
-
bioRxiv - Neuroscience 2022Quote: ... primary antibody overnight at 4 °C and revealed with a biotinylated goat anti-rabbit IgG secondary antibody (1:1,000, 2 hat RT, Vector Laboratories, VECTBA1000), the VECTASTAIN ABC Elite HRP kit (Vector Laboratories ...
-
bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 in blocking solution at 4 °C overnight followed by incubation with peroxidase-linked goat anti-rabbit IgG (1:10000, # PI-1000-1, Vector Laboratories). Chemiluminescent substrate (# 34095 ...
-
bioRxiv - Physiology 2024Quote: ... was incubated at 4°C overnight and a biotinylated secondary antibody followed by streptavidin-DyLight 594 (BA1000 and SA-5594, Vector Laboratories) were used for cardiomyocyte identification ...
-
bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated first with a Biotinylated Goat Anti-Rabbit IgG Antibody (over-night at 4°C-Vector Laboratories), and then with Alexa Fluor™ 594 streptavidin (3 hours – room temperature-Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The polyps were kept for at least o/n at 4°C in a Vectashield mounting medium with DAPI (Vector Laboratories) before being mounted and imaged on a Leica SP8 confocal system ...
-
bioRxiv - Developmental Biology 2024Quote: ... samples were washed three times in wash buffer for 20 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs). Images were acquired using a Leica Stellaris8 confocal microscope with a 63X oil immersion objective lens (NA = 1.4 ...
-
bioRxiv - Genetics 2024Quote: ... samples were washed three times in the wash buffer for 20 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs). All solutions used for RNA FISH were RNase-free ...
-
bioRxiv - Genetics 2022Quote: ... samples were washed two times in wash buffer for 30 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs).
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibodies for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... was followed by incubation with the primary antibody (Table S2) at 4°C for 24 or 48h in 2% (vol/vol) serum and with the corresponding biotinylated antibody (Vector Laboratories) or alkaline-phosphatase antibody (ab6722 ...
-
bioRxiv - Microbiology 2024Quote: ... The membrane proteins were separated by centrifugation at 104,000 g for 1 h at 4 °C and loaded onto a column prefilled with either 2 mL of agarose-bound WFA (Vector Laboratories) for S ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies for 48 h at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 h at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... (recognizing Aβ and C99 but not holo-APP) or for 30 min in citric acid solution pH=6 at 90-100°C (Pressure cooker, Vector Laboratories) for 82E1 and anti-Iba1 (1/1,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... blocked with 3% NDS-PB-TX and first incubated in biotinylated secondary anti-mouse antibody (1:200, Vector Labs, CAMK-IIα amplification) overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Slides were washed 3×5 min in PBS and incubated with 1:1000 anti-rabbit biotinylated secondary antibody (BA-1000, Vector Laboratories, USA) in 1% NGS in PBST for 1 h at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3x in 0.1M PBS for 10 min per wash and horseradish peroxidase was visualized using 3,3’ diaminobenzidine (DAB) in a 3 M sodium acetate buffer containing 2.5% nickel sulfate and 0.05% H2O2 (SK-4100, Vector Laboratories, Inc, Burlingame, CA) for 3 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... in phosphate buffer 0.2 % Triton supplemented with10 % horse serum and rinsed 3 more times 10 min before being mounted in Vectashield (Vector Labs, H-1000). For Phalloidin staining ...
-
bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were washed again (3 times in PBS/Tween, 5 minutes each) and incubated in biotinylated goat anti-rabbit (1: 200, Vector Laboratories, USA) or biotinylated horse anti-mouse (1 ...
-
bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times for 5 minutes with PBS and mounted using VECTASHIELD Antifade Mounting Medium (Vector Laboratories, H-1000) or Prolong Gold Antifade Mountant with DAPI (Thermo Fisher ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA) followed by Vectastain Elite ABC HRP (Vector labs ...
-
bioRxiv - Cell Biology 2021Quote: ... Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100). Slides were rinsed with water and counterstained with Hematoxylin (RICCA Chemical Company ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cuticles were washed 3 times in PBSS before mounting on glass slides in Vectashield mounting media without DAPI (Vector Laboratories, Inc.). Tumours were stained overnight at 4°C with DAPI ...
-
bioRxiv - Plant Biology 2022Quote: ... the slides were dehydrated in 70-90-100% ethanol series for 3 min each and counterstained with 10 µg/ml 4’,6-diamidino-2-phenylindoline (DAPI) in Vectashield antifade medium (Vector Laboratories, USA). For immunodetection of microtubules ...
-
bioRxiv - Neuroscience 2022Quote: ... free-floating sections were washed with PBS 3 times and blocked in 5% normal goat serum (NGS, Vector Laboratories, S-1000-20) and 0.1% Triton X-100 (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were washed for 3 times in 0.1 M PBS and mounted on glass slides using Vectashield medium containing DAPI (Vector Labs, Peterborough, UK).