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Citations for Vector Labs :
201 - 250 of 381 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Unmasking of the epitopes was performed at 100°C for 20 min in Citrate-based Antigen Unmasking Solution (Vector Laboratories). Sections were incubated for 30 min at room temperature in blocking buffer (3% BSA in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... left to dry at 60°C for 30 minutes before mounting with VectaMount Permanent Mounting Medium (Vector Laboratories, H-5000). Slides were scanned using a Nanozoomer-XR Digital Slide Scanner (Hamamatsu ...
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bioRxiv - Neuroscience 2020Quote: ... They were finally washed 4 × 15 min in RT 0.3% PBT and placed in VectaShield at 4 °C overnight before imaging (Vector Laboratories). trans-Tango and GRASP analyses required separate staining of neuropil and respective fluorophores due to different incubation times.
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bioRxiv - Immunology 2022Quote: ... The cells were washed and stained for 30 min at 4°C with 5 μg/ml FITC- WGA (Vector Laboratories). After the wash ...
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bioRxiv - Physiology 2022Quote: ... Permeabilized retinas were incubated overnight at 4°C with biotinylated Isolectin B4 (IB4; Vector Laboratories, B-1205, diluted 1:500). Retina were imaged using a Zeiss LSM 780 GaAsp inverted laser scanning fluorescence microscope ...
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bioRxiv - Developmental Biology 2022Quote: ... left to dry at 60°C for 30 min before mounting with VectaMount Permanent Mounting Medium (Vector Laboratories, H-5000).
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bioRxiv - Biophysics 2024Quote: ... The co-culture was incubated overnight at 4 °C with 10 µg/ml of Tomato Lectin (Vector Laboratories, #DL-1178) in blocking solution ...
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bioRxiv - Biophysics 2023Quote: ... The co-culture was incubated overnight at 4 °C with 10 mg/ml of Tomato Lectin (Vector Laboratories, #DL-1178) in blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was performed at 60°C in citrate-based antigen unmasking solution diluted 1:100 (Vector Labs, Burlingame, CA) for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were counterstained with Nissl method (0.5% cresyl violet solution at 37°C for 10 minutes) to visualize anatomical landmarks and mounted with Vectamount (Vector Labs) to use for image analysis.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was completed for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the sections were incubated with anti-rabbit secondary antibody (1:200 dilution; 4°C; 17h.; Vector Laboratories, Burlingame, USA) followed by tertiary labelling with avidin biotin complex (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed 3 times with cold PBS and resuspended in Vectashield mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Cells were imaged at 100X magnification using a Leica DM IRBE microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS for 3 times and mounted with VECTASHIELD antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, H-1200; Vector Laboratories). Images were taken by SP8 confocal microscopy (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour at room temperature in 3% normal donkey serum (NDS, Vector Laboratories, Inc., Burlingame, CA, USA) and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Immunology 2022Quote: ... On day 3 secondary antibodies were added and visualised with the Vector Red Alkaline Phosphatase Substrate Kit (Vector Laboratories #SK-5100). For neutrophil elastase (NE ...
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated for 5 hours at room temperature in VectaFluor DyLight 594 anti-mouse secondary antibody (3 drops in 5mL of 0.1% BSA, DK-8818; Vector Labs, CA). When incubation was completed ...
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bioRxiv - Neuroscience 2021Quote: ... and later in 0.1 M PB (3 × 20 min) and mounted on glass slides with Vectashield mounting medium (Vector Lab, Inc.). The characterizations of primary antibodies used in humans ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed 3 times in PBS and the secondary antibody (biotinylated horse anti-rabbit; 1:250, Vector Laboratories, Burlingame, CA) was added and slides were incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were rinsed 3 times with PBS and mounted on glass slides using Vectorshield antifade mounting medium (H-1000, Vector Laboratories) and cover slipped.
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bioRxiv - Neuroscience 2022Quote: ... The sections were then washed 3 times with 1xPBS for 10 min each and mounted using Vectashield Mounting media (Vector laboratories). The slides were imaged using a Leica SP5 multiphoton confocal microscope and Olympus FluoView FV1000.
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bioRxiv - Bioengineering 2022Quote: ... Slides were then washed 3 times for 10 minutes each in PBS and coverslips were applied with Vectashield antifade mounting medium (Vector Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were washed 3 times for 5 min with PBS-T and incubated with 60 μg/ml PSA-FITC (FL 1051, Vector Laboratories) in PBS for 30 min in dark at room temperature for acrosome labelling ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in PBS three times for 5 min each before being placed in a blocking solution containing 3% normal goat serum (Vector Laboratories) for 1 hr ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed 3 times in washing solution and stored in PBS or mounted with coverslips using Vectashield Antifade Mounting Medium (Vector Laboratories).
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bioRxiv - Bioengineering 2020Quote: ... Cells were again washed 3 times in PBST and incubated with FITC- or Texas Red-conjugated streptavidin in blocking buffer (1:50, Vector Laboratories). Where appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... endogenous peroxidase activity was blocked by treating sections with 3% H2O2 and antigens were retrieved with Antigen Unmasking Solution (Vector Laboratories). Tissues were blocked in 10% serum with 0.25% Tween 20 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed and stained with biotinylated anti-mouse IgG (Table 3) in PBST 1 % BSA for 1 h at RT before washing and addition of ABC Elite amplification (Vector Labs) for 30 min at RT according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed an additional 3 × 5 minutes with PBS before adding mounting medium containing DAPI counterstain (Vector labs, H-1200) and applying glass coverslips ...
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bioRxiv - Cell Biology 2022Quote: ... Intestine sections were rehydrated and antigens were unmasked for 30 min in a steamer with sodium citrate (10 mM, pH 6) (Ki67, GFP, p57, PCNA, cleaved caspase 3) or High pH (H-3301, Vector Laboratories) (Olfm4 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Physiology 2024Quote: ... slides were rinsed 3 times in Tris-Buffered Saline (TBS) and then incubated in BLOXALL Endogenous Enzyme Blocking Solution (cat# SP-6000, Vector Laboratories) for 15 min in dark to quench endogenous peroxidase ...
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bioRxiv - Microbiology 2024Quote: ... The cysts were washed 3 times with 1x PBS and mounted in VECTASHIELD® Antifade Mounting Medium (Vector Laboratories, Newark, CA). Samples were illuminated using 380nm (CFW) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed 3 times with 1x PBS and mounted in VECTASHIELD® Antifade Mounting Medium (Vector Laboratories, Newark, CA). Samples were illuminated using 380 nm (CFW) ...
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bioRxiv - Physiology 2024Quote: ... Slides were then dried for 3 min at RT and circled with a Hydrophobic Barrier Pen (Vector Laboratories, GZ-93951-68) before 30 min protein digestion (protease IV ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were washed in PBST-2 for 3 × 20 minutes at room temperature in the dark and mounted on a microscope slide using Vectashield mounting media (Vector Labs). To maintain the 3-dimensional structure of the brain ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed 3 x 5 mins in PBST and incubated with fluorescein isothiocyanate-conjugated streptavidin (1:50; Vector Laboratories) in blocking buffer for 30 mins at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and mounted onto glass slides with DAPI nuclei stain VECTASHIELD mountant (Vector Laboratories). Samples were visualised on Evos Cell imaging system (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then wash 3 times for ten minutes each in TBST and then incubated with prepared elite avidin biotin complex (ABC) reagent (Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Another 3 x 10 minute washes in PBST were completed before mounting brains on a microscope slide with vectashield (Vector Laboratories) for imaging ...
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bioRxiv - Genetics 2022Quote: ... Brains were then washed a final 3 times in PBS and mounted in Vectashield mounting medium (Vector Labs, H-1000-10) for imaging ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then rinsed 3 times for 10 minutes each with PBS and were subsequently treated with DAPI (Vector Labs) at a 1:5,000 dilution for 5 minutes and washed in PBS 3 times ...
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bioRxiv - Cell Biology 2022Quote: ... Sprouts were visualized from days 3-4 via confocal imaging after overnight incubation with FITC labeled Ulex europaeus lectin (Vector labs). Immunofluorescence imaging was performed on a Yokogawa CSU-W1 spinning disk confocal microscope with 20x ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were then rinsed using 1x PBS for 3 times before mounting on glass slides with the Vectorshield antifade mounting medium (H-1000, Vector Laboratories). Slides were left at room temperature overnight before sealing with nail oil and imaging ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections with PBS (3 x 10 min washes) and incubated the sections with avidin-biotin-peroxidase complex (ABC, ABC Elite Kit, #PK6100, Vector Laboratories) for 1 h at room temperature ...