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Citations for Vector Labs :
2301 - 2350 of 2431 citations for Mouse Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sections were again rinsed in PBS and then developed in a solution containing the chromogen [Nova Red peroxidase (HRP) substrate kit (SK-4800, Vector Laboratories)] according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Development of the biotinilated antibody signal was done by 1 h incubation with the Avidin/Biotin-HRP complex (Elite Vectastain ABComplex Kit, Vector Laboratories) following 1 h incubation with Streptavidin-647 (BioLegend) ...
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bioRxiv - Genomics 2022Quote: ... permeabilized using 0.1% Triton-X and processed for CDH1 (E-Cadherin) staining using the VIP peroxidase substrate kit (Vector labs SK4600) and anti-mouse E-Cadherin primary antibody (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... the first visualization was followed by a blockade of avidins and biotins using avidin-biotin blocking Kit (SP-2001; Vector Laboratories) for 15 min prior to an additional blocking and permeabilization step ...
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bioRxiv - Neuroscience 2023Quote: ... the immunoproduct was visualized by a last incubation in the substrate of the Vector SG Peroxidase Substrate Kit (SK-4700; Vector laboratories). After final rinses ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS and blocked and stained using appropriate primary antibodies and reagents from the Vectastain Elite ABC kit (Vector Labs). Diaminobenzidine exposure was titrated to optimal contrast ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed (0.1M PBS and 0.1M PB) and incubated with Vectastain Elite ABC-HRP Kit (1:200; Vector Laboratories PK-6100). Sections were developed for 5 min in DAB solution (3,3’diaminobenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... Images were analyzed and edited with ImageJ 2.0.0. (NIH). HRP-labelled sections were revealed (approx. 1 min) using the Diaminobenzidine (DAB) Substrate Kit (Vector Laboratories, SK-4100). The enzymatic reaction was stopped with ddH2O and samples were counterstained with Harry’s hematoxylin (15 s ...
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bioRxiv - Systems Biology 2023Quote: ... Biotin-conjugated antibodies were prepared 100µg at a time using the ChromaLINK One-Shot Antibody Biotinylation Kit (Vector labs, Newark, USA).
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bioRxiv - Physiology 2023Quote: ... of paraffin-embedded tissues using specific commercially available primary antibodies (Supplementary Table S1) and AlexaFluor 488 and 568-conjugated F(ab’) secondary antibody (Molecular Probe) or VECTASTAIN ABC kit (Vector lab). Cell-specific CD68 positive and total cell numbers were counted in the area of 0.07244 mm2 ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were rinsed with Wash Solution before application of a blocking solution from an anti-rat ImmPRESS Detection kit (#MP7444-15, Vector Labs) for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then washed (5 x 5 min, 20 rpm) and processed with avidin-biotinylated horseradish peroxidase complex (Vectastain Elite Kit, Vector Laboratories) in 0.3% PBST for 1 h at room temperature (2 rpm) ...
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bioRxiv - Cancer Biology 2023Quote: ... paraffin-embedded tumour-containing lungs were used for immunohistochemical (IHC) analysis of xCT using a VECTOR DAB substrate kit (Vector laboratories) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the sections were washed in PBST and incubated for 90-120 min in the avidin-biotin-peroxidase complex (ABC Elite kit, PK-610, Vector Laboratories) in PBST ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections with PBS (3 x 10 min washes) and incubated the sections with avidin-biotin-peroxidase complex (ABC, ABC Elite Kit, #PK6100, Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by additional rinses and an overnight incubation in avidin biotin complex (Vectastain Elite ABC kit HRP, standard, cat#PK-6100, Vector Laboratories). The next day sections were rinsed in PBS and incubated in a solution containing 3’3’-diaminobezidine tetrahydrochloride hydrate (DAB ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated for 90 minutes with appropriate secondary pre-diluted antibody from the Vectastain ABC kit (Vector Laboratories Ltd, UK).
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bioRxiv - Neuroscience 2024Quote: ... We then washed the sections three times with PBS (10 min each) and incubated them for 1 hour in avidin-biotin-complex (Vectastain ABC Elite Kit; Vector Laboratories). We washed sections four times in PBS (5 min each ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunocytochemistry was performed on differentiating myoblasts using the myogenin or MHC hybridoma supernatant and was developed with the universal ABC kit (Vector Labs) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were again washed 3 times (15 minutes) in TBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories) for 2 hours at room temperature before additional washes in TBS (2 times ...
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bioRxiv - Neuroscience 2021Quote: ... signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100, ABC Vectastain Elite kit, Vector Laboratories, UK). Chromogen development was sequential with double labelling ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were subjected to the staining protocol of the Vectastain Universal Elite ABC Kit (PK-6200, Vector Laboratories, Inc., Burlingame, CA). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... unspecific bindings (5% BSA, 5% NGS in PBST, 1h) and Avidin/Biotin (Avidin/Biotin Blocking Kit, Vector Laboratories, SP-2001, 15 min). Samples were incubated overnight at 4°C with anti-MDA antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... and BrdU (mice, AbD Serotec) were detected with biotinylated secondary antibodies (Jackson) and the Vector-Elite ABC kit (Vector Laboratories, Burlingame, CA). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories, PK-6100) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Another PBS/TWEEN washing battery was performed and sections were then incubated in an avidin-biotin-peroxidase complex (ABC kit, Vector Laboratories®) for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800; Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with a monoclonal secondary horseradish peroxidase (HRP)-coupled horse-anti-rabbit antibody (ImmPRESS Reagent Kit, MP-7401, Vector Laboratories, Germany) with AEC+ (K346 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed again three times in PBS and the 3,3’-diaminobenzidine (DAB) chromogen DAB Peroxidase (HRP) Substrate Kit (Vector Laboratories, Burlingame, CA) prepared immediately prior to use and addition to each slide individually ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 1h incubation at room temperature with a 1:250 dilution of an avidin-biotin complex solution (ABC Vectastain kit, Vector Laboratories®). Revelation was performed using the DAB Peroxidase Substrate Kit (DAB SK4100 kit ...
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bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... the sections were immunostained by the avidin–biotin peroxidase method using the Vectastain Elite Kit (PK-6102, Vector Laboratories, Burlingame, CA, USA) with 3–3-diaminobenzidine and hydrogen peroxide as the chromogen ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Physiology 2020Quote: ... Signal amplification was performed using an ABC Kit (Vectastain, #PK6100) for 30 min at RT followed by DAB (Vector Labs, #SK-4100) sub-strate ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was performed by boiling for 20 min in a 0.01 M sodium citrate (pH 6.0) solution and endogenous biotin was blocked using the Avidin/Biotin Blocking Kit (Vector Labs, SP-2001). Slides were incubated overnight with primary antibodies against anti-rabbit H3K4me3 (Cell Signaling Technology ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Non-specific binding sites were blocked for 1 hour (Col2: MoM Kit, Vector Labs, BMK-2202; Sox9: 10% v/v goat serum). Primary antibodies were incubated overnight at 4°C and included Col2 (1/100 ...
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bioRxiv - Cell Biology 2021Quote: ... Chromogen development was then conducted with HRP 3,3’-Diaminobenzidine (DAB) or Vector Blue kits (Vector Laboratories, Cat # SK-4100 or SK-5300). Chromogen development was halted by submersion in tap water ...
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bioRxiv - Cell Biology 2021Quote: ... before peroxidase staining was conducted for 30 min with a peroxidase staining kit (VECTASTAIN® ABC Reagent, cat # PK-4000, Vector Laboratories) as described by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated with polymer-HRP-conjugated horse anti-goat IgG antibody (ImmPRESS™ HRP Anti-Goat IgG Polymer Detection Kit, Vector Laboratories) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... and nonspecific epitopes (species-specific serum diluted 1:5 in Tris-buffered saline and 5% bovine serum albumin, or blocking serum from species specific ImmPRESS® kit; Vector Laboratories) and incubated with primary antibody (Table 2 ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and finally an avidin-biotin-peroxidase complex (ABC kit standard, PK-6100, Vectastain, Vector Laboratories, Burlingame, CA, USA; 1:250; 1 h). Peroxidase activity was detected using a solution containing 0.02 % 3,3’-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and blocked with 10% goat or rabbit serum in PBS for 60 min and with Avidin/Biotin Blocking Kit (Vector Laboratories, Inc.) for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... The Aβ 42/40 double-labelled samples were incubated in ImmPRESS horseradish peroxidase polymer then ImmPRESS alkaline phosphate polymer kits for 30 mins each (Vector Laboratories, US). For these sections ...
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bioRxiv - Neuroscience 2024Quote: Slices containing biocytin fills were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer overnight and processed with Vectastain ABC (avidin-biotinylated HRP complex) Elite Kit (Vector Labs, Burlingame, CA). The shape of the slice ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc.) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by blocking of nonspecific binding sites using normal goat serum (10%) and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA). Sections were incubated with phospho-SMAD2/3 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... Engineered cartilage sheet sections were also treated with pronase and primary anti-Collagen Type II (DSHB II-II6B3) followed by VectaFluor R.T.U Antibody Kit DyLight® 488 (Vector Labs DI-2788) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG, Vector Laboratories, PK-4004). Antibody binding was visualized with DAB-nickel (VWR ...
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bioRxiv - Physiology 2023Quote: ... Another block was applied with streptavidin and then biotin solutions (Streptavidin/Biotin Blocking Kit, Vector Labs; Newark, CA, USA; Cat. No.: SP2002) at room temperature for 15 min each ...