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Citations for Vector Labs :
2201 - 2250 of 2431 citations for Mouse Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... while ΔFosB required DAB revelation for best results (BA-2000 secondary antibody, 1:250, and PK6100 kit from Vector Laboratories) (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... this was followed by an incubation in avidin–biotinylated horseradish peroxidase complex (Vectastain Elite ABC kit; 1:300; Vector Laboratories) for 3 h at room temperature (RT ...
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bioRxiv - Cell Biology 2021Quote: ... For Lotus Tetragonolobus Lectin (LTL biotinylated) staining sections were additionally blocked with a streptavidin/biotin blocking kit (both Vector Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Tissue sections were fixed with ice cold acetone for 5 min and blocked with Avidin/Biotin Blocking Kit (Vector laboratories) and Protein block (Protein block (Dako ...
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bioRxiv - Neuroscience 2021Quote: ... At the end of the experiment the slice was fixed and processed with an ABC kit (Vector Laboratories, Burlingame, CA), allowing recovery of the morphologies of the recorded neurons.
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit; Vector Laboratories) for 1 hour in the dark at room temperature (RT) ...
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bioRxiv - Developmental Biology 2022Quote: ... and Ki67 immunostaining signals were further amplified by tyramide-FITC or tyramide-Cy3 (1:2,000, Perkin-Elmer, Wellesley, MA) after avidin-biotin complex incubation (Elite ABC kit, Vector Laboratories). Immunostained sections were counterstained with DAPI (1 μg /ml ...
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Necrosis reduction efficacy of subdermal biomaterial mediated oxygen delivery in ischemic skin flapsbioRxiv - Bioengineering 2022Quote: ... Each slide was washed with PBS (pH 7.4) and incubated with a secondary antibody using the Vectastain ABC kit (Vector Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... sections were incubated with biotinylated goat anti-rabbit secondary antibody for 1.5 hours at room temperature followed by incubation with the VECTASTAIN Elite ABC HRP Kit (Vector Labs) and the DAB HRP Substrate Kit (Vector Labs) ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8) slices were treated with a peroxidase-based enzymatic detection system (Vectastain Elite ABC kit (PK-6100, Vector Laboratories)) ...
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bioRxiv - Cancer Biology 2022Quote: ... and analyzed by lectin blotting using biotinylated concanavalin A (Con A) lectin (1:10,000, Cosmo Bio) and VECTASTAIN ABC-HRP kit (PK-4000, Vector Laboratories) or by Western blotting using primary antibodies and secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... The stained sections were then developed using immPACT DAB Peroxidase (HRP) Substrate Kit (Cat# SK-4150, Vector Labs, CA, USA), Vectastain ABC-AP kit (Cat#AK-5000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then treated for one hour at RT with the VECTASTAIN ABC kit (PK-6100; Vector laboratories, Burlingame, CA) for avidin binding and peroxidation ...
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bioRxiv - Cancer Biology 2024Quote: ... were applied for 30-60 minutes at room temperature and visualized with DAB substrate (ImmPACT kit, Vector Laboratories #SK-4105). Tissues were then counterstained with hematoxylin ...
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bioRxiv - Cell Biology 2023Quote: ... Alkaline Phosphatase (ALP) staining was performed on live cells using VECTOR Red ALP substrate kit (Vector Laboratories Cat#SK-5100) with a 30-minute incubation ...
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bioRxiv - Cell Biology 2023Quote: Autofluorescence in immunofluorescence staining was quenched using the Vector® TrueVIEW® Autofluorescence Quenching Kit (Vector Laboratories, SP-8400-15) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Then the immunosignals were detected by HRP-conjugated secondary antibodies and the DAB substrate using an ABC kit (Vector lab). IHC images were acquired with a Leica CTR 5500 fluorescence ...
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bioRxiv - Pathology 2023Quote: ... and 3,3’-Diaminobenzidine tetrahydrochloride (DAB, Carl Roth) as previously described 34 or using avidin–biotin complex (ABC) peroxidase kit (Vector Labs) and DAB (Carl Roth ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Autofluorescence of aortic tissue was quenched using the Vector TrueVIEW Autofluorescence Quenching Kit (VEC-SP-8400, Vector Laboratories, Newark, USA). Slides were incubated for 5 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... followed by 1 h incubation with ImmPRESS VR anti- rabbit IgG HRP polymer detection kit (MP-6401-15; Vector Laboratories). Chromogenic detection in situ was performed using ImmPACT DAB EqV peroxidase substrate solution (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% normal goat serum followed by an Avidin/Biotin blocking kit (Vector Labs, Burlingame, CA, USA) and then incubated overnight at room temperature with the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Auto-fluorescence was quenched following the manufacturer’s instructions of the Vector TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, #SP-8400-15). The slides were stained in DAPI (50 ng/mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Slides were then incubated with 2.5% normal horse serum from the ImmPRESS Horse Anti-Rabbit IgG Polymer Kit (catalog no. MP-7401, Vector Laboratories) for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the N 1C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... DAB was used as chromogenic method of detection and signal was developed using the VECTASTAIN Elite ABC HRP Kit (PK-6100; Vector laboratories) combined with ImmPACT DAB Peroxidase (SK-4105 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% TritonX-100 in PBS) for 30 min at RT followed by another blocking using M.O.M kit (Vector Laboratories BMK-2202). Primary antibodies (mouse anti-MYH3 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in biotinylated secondary antibody diluted 1:500 in block (biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories). Sections were washed in TBS and then incubated for 1 hour at RT in Vectastain kit components A and B ...
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bioRxiv - Molecular Biology 2021Quote: ... which was then tipped off for blocking the endogenous biotin suing the Avidin Biotin blocking kit from Vector labs (SP-2001). Sections were then fixed for 30 minutes with 10% Neutral buffered formalin (VWR-89370-094) ...
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bioRxiv - Immunology 2022Quote: ... On day 3 secondary antibodies were added and visualised with the Vector Red Alkaline Phosphatase Substrate Kit (Vector Laboratories #SK-5100). For neutrophil elastase (NE ...
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bioRxiv - Bioengineering 2019Quote: ... IHC staining was carried out using a standard protocol from Vectastain ABC Elite kit (Vector Labs, Burlingame, CA cat#PK-6101). Vector NovaRED (Vector Labs ...
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bioRxiv - Cancer Biology 2019Quote: ... 1:1000 in TBS with 1% BSA) for 1 hour at room temperature and developed with a 3,3-diaminobenzidine kit (Vector Laboratories, USA). Separate slides were counterstained with hematoxylin (Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Recognition of glycosylated sorLA by lectins was carried out applying biotinylated lectins (10 μg/mL; Biotinylated Lectin Kit I and Biotinylated SNA, Vector Laboratories) and peroxidase-conjugated streptavidin (Calbiochem ...
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bioRxiv - Pathology 2019Quote: ... slides were washed in PBS and the biotinylated rabbit antibody (1:200 dilution, VECTASTAIN® Elite® ABC Kit, Vector Laboratories) was applied in blocking solution for 1 h ...
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bioRxiv - Pathology 2020Quote: ... and a blue reaction product was produced by incubating sections with alkaline substrate (Vector blue AP substrate kit III, Vector laboratories) for 20 to 40 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... was carried out using a biotinylated secondary antibody together with the avidin-biotin system and the VECTOR peroxidase kit (PK-6100, Vector Laboratories) using diaminobenzidine tablets as a substrate.
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bioRxiv - Physiology 2021Quote: ... Slides were incubated with 6% H2O2 for 30 min after primary antibody incubation and were then biotinylated with a rabbit IgG and ABC kit (Vector Labs) for immunohistochemistry ...
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bioRxiv - Bioengineering 2020Quote: ... Three drops of normal goat serum in 10ml PBS was used as blocking buffer for 1 hr at room temperature before primary antibodies incubation (Vectastatin ABC Kit; Vector Laboratories). Slides were then incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... MPBiomedicals, 1:5000) and collagen type II (No. 0863171, MPBiomedicals, 1:1000) was performed using the Vectastain ABC kit (Vector Labs) with hematoxylin counterstaining as in standard protocols (Scotti et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were processed the following day using a Vectastain kit (biotinylated goat-anti-rabbit igG secondary antibody and avidin-biotin-peroxidase complex) and ImpacDAB (Vector Laboratories). Control sections lacking the primary antibody were unlabeled ...
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bioRxiv - Genetics 2021Quote: ... then blocked with 5% (v/v) horse serum (ImmPRESS HRP Anti-Rabbit IgG [Peroxidase] Polymer Detection Kit; Vector labs; MP-7401). Sections were incubated with rabbit anti-TCFL5 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with anti-rat biotinylated secondary antibody (2h, room temperature) and processed with avidin-biotin-peroxydase complex (ABC Elite Kit, Vector Laboratories) for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNMT3A and DNMT3B was visualized in cross-section (5 µm) of paraffin-embedded tissues using specific primary antibodies and a Vectastain Elite ABC Kit (Vector Laboratories) or AlexaFluor 488-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... sections were incubated with biotinylated secondary antibodies and the signal was revealed using the Vectastain Elite ABC-HRP peroxidase kit and the ImmPACT NovaRED HRP substrate (Vector laboratories). Counterstaining was performed using 50% Harris hematoxylin for 30 seconds followed by incubation in 0.1% sodium bicarbonate solution for 1 minute at room temperature.
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 1-hour in an avidin biotin complex solution (per kit instructions, ABC Peroxidase, PK-6100, Vector Laboratories) at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were rinsed with wash buffer before all sections had the blocking solution applied from the Rat ImmPRESS kit (MP7444-15, Vector Labs) for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The wells were then washed twice with PBS and reacted with avidin/biotin-HRP complex (VECTASTAIN Elite ABC kit; Vector Laboratories) at 50 μL/well at 4 °C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were then rinsed and incubated in ABC solution (PBS: A: B=50:1:1) (Vectastain ABC kit, Vector Laboratories, Inc.) for 30 min at rt ...
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bioRxiv - Neuroscience 2021Quote: ... We rinsed the sections again in PBS and incubated them in avidin–biotin– peroxidase complex (ABC Elite kit, PK‐6100, Vector Laboratories) in 0.5% PBS‐TX for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoperoxidase detection was then performed by enzymatic reaction with an avidin-peroxidase complex for 1 h (Vectastain ABC HRP Kit Peroxidase Standard, Vector Lab.). After washing in PBS ...
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bioRxiv - Immunology 2021Quote: ... Color was developed by incubating the slides with freshly prepared avidin-biotin-peroxidase complex (ABC) solution (Vectastain Elite ABC Kit; Vector Laboratories), followed by exposure to 3-amino-9-ethylcarbazole substrate (AEC ...