Labshake search
Citations for Vector Labs :
1851 - 1900 of 1955 citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with biotinylated goat anti-rabbit secondary antibody (1:600 dilution; Vector Laboratories, catalog #BA-1000) in PBS-Tx and 1% NGS for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories, Burlingame, CA). Slides were developed with Bio-Red (Biopath ...
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and stained overnight at 4C with FITC-conjugated IB4 (Fluorescein labeled Griffonia Simplicifolia Lectin I isolectin B4, FL-1201, Vector laboratories, 1:200) or primary antibody against Cld-5 (mouse monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... or the corresponding secondary antibodies: biotinylated goat anti-guinea pig antibody (1:100 dilution for GFP detection; Vector Laboratories, Cat# PK-4007; RRID:AB_2336816) and secondary antibody goat anti-mouse IgG coupled to 1.4 nm gold (1:100 dilution for TH detection ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed with PBS and incubated with the following blocking solutions for 1 hour at room temperature: Mouse Ig Blocking Reagent in Mouse on Mouse (MOM) kit from Vector Laboratories (PK-2200) for staining with antibodies against Cbl (mouse monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... USA) followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Cat. No. BA-1000; Vector Labs Inc., Burlingame, CA, USA). Controls for nonspecific binding consisted in omitting the primary antibody (S Fig 5) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with anti-mouse Ig-fluorescein antibody (1:200) and mounted on glass slides using DAPI (Vectashield, Vector Laboratories, CA, USA). Images were acquired in 40X magnification using a fluorescence microscope BX53 (Olympus Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in a solution containing avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories; 1:50) for 2hrs ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: ... were used as described previously (Kantar et al., 2021) for 1 hour at room temperature before mounting the coverslips with Vectashield (Vector Laboratories #H-1200) and imaging on Zeiss Apotome or Leica Thunder microscopes.
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 × 10 min with PBS and incubated in a tertiary of avidin and biotinylated horseradish peroxidase (1:1000; ABC kit, Vector Labs, PK-6100) and stained with a 3 ...
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bioRxiv - Neuroscience 2022Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Cancer Biology 2022Quote: ... CDX tumors were grown to approximately 750 mm3 and mice received an i.v injection of Biotinylated Tomato Lectin (4mg/kg, Vector Laboratories, B-1175-1) one hour before they were sacrificed by Schedule 1 method ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used for immunohistochemistry included biotinylated goat anti-rabbit (BA-1000) and biotinylated horse anti-goat (BA-9500) at a concentration of 1:200 (Vector laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated in the presence of biotinylated goat anti-rabbit IgG followed by Vectastain elite ABC reagent (1:600, #PK6101, Vector Laboratories, Burlingame, USA). Fos-like immunoreactive cells were visualized with the avidin-biotin peroxidase method using diaminobenzidine as a chromogen ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... at 1:500 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500).
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... the slides were incubated with horse biotinylated anti-mouse/ rabbit IgG ABC-HRP peroxidase at room temperature for 1 hr using the VECTASTAIN Elite ABC Universal Kit (Vector Laboratories, PK-6200). The slides were then washed with 1X PBS for 10 min at room temperature and incubated with VECTASTATIN Elite ABC reagent (Vector Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... Biotin-conjugated anti-goat or anti-rabbit secondary antibodies were each applied at 1:500 dilution and incubated with Vector elite Reagent (Vector Laboratories, CA, USA) for 45 minutes ...
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bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed and then incubated in secondary antibody (Alexa 488) for 1 h at room temperature and mounted using VECTASHIELD with DAPI (VECTOR Laboratories Ltd, UK) and coverslips.
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Physiology 2024Quote: ... The tissues were counterstained with 1 μg/ml DAPI and slides were mounted in Vectashield Vibrance antifade mounting media (Vector Laboratories, H-1700). Stained tissues were imaged on confocal Zeiss 710 microscope operated by ZEN software (Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at RT and initiation of color reaction with a Peroxidase Substrate Kit (SG Peroxidase Substrate Kit, Vector Laboratories, SK-4700). All stained sections were mounted ...
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bioRxiv - Neuroscience 2023Quote: ... labeled with rabbit anti-ionized calcium-binding adapter molecule (Iba)-1 antibody (Wako Chemicals, Neuss, Germany) and VectaStain Elite ABC-HRP kit (Cat.-No.: PK-6100, Vector Laboratories, Burlingame, USA), and visualized with diaminobenzidine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies with their respective concentrations were as follows Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 (1:25, Vector Laboratories, #FL-1201), mouse monoclonal anti-3G5 IgM (1:10 ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:200 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500). Imaging was performed on a Leica SP8 Confocal microscope using a 40x oil objective ...
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bioRxiv - Developmental Biology 2022Quote: ... Ki67 staining was performed using monoclonal rabbit anti-Ki67 antibody (1:100, Linaris, Dossenheim, Germany) and signals were amplified by the use of the Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Excess antibody buffer was removed with PBSTx and followed by incubation in biotinylated goat anti-mouse secondary antibody (1:1000 Cat# BP-9200-50: Vector Laboratories, California USA) overnight at 4°C in PBSTx and 1% NGS ...
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bioRxiv - Microbiology 2022Quote: ... brain sections were stained with rabbit anti-SINV capsid antibody (1:5000 dilution) and the Vectastain Elite ABC HRP kit (Vector Laboratories, PK-6101) was used for signal detection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and stained with 1:20 diluted fluorescein-conjugated UEA-I (Lectin Kit I, Fluorescein, UEA-I-FL, #FKL2100; Vector Laboratories, Burlingame, CA, USA). The cells were washed with PBS containing 0.2% FBS and subjected to flow cytometry by a Moxi GO II cell analyzer (Orflo ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody incubation was with horse anti-mouse IgG conjugated to biotin (1:100 in PBS; Vector Labs, catalog BA-2000-1.5) for 2 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Retinas were then washed 3 times in PBS at RT and incubated with polyclonal rabbit LM opsin and S opsin antibody (1/300; Vector Laboratories, Peterborough, UK) for 48 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... the sections were incubated for 2 h in biotinylated goat antirabbit secondary antibody diluted 1:500 in 0.01 M PBS and subsequently incubated with ABC reagents (Standard Vectastain ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) for 20 min in the dark at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were blocked with BSA in PBS-Tween and antibodies were incubated at the following dilutions: anti-Biotin (1:1000, Vector Labs, SP-3000), anti-RNA polymerase II CTD repeat YSPTSPS [phospho S5] [4H8] (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... the slides were incubated with secondary antibodies for 1 hour and washed again 3 times with PBS and mounted with Vectaschield containing DAPI (Vector Laboratories, H-1500).
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bioRxiv - Immunology 2024Quote: ... diluted 1:30 was applied for 15 minutes followed by biotinylated secondary rabbit anti-rat antibody diluted 1:400 (BA-4001; Vector Laboratories, Burlingame, CA) for 10 minutes and then ready-to-use Bond polymer refine detection kit with 3,3’- diaminobenzidine (DAB ...
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bioRxiv - Pathology 2024Quote: ... antibodies were coadministered overnight and were labeled with horse anti-rabbit or anti-goat Dylight 488 and 594 (Vector Laboratories, 1:300 dilution) secondary antibodies for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two hours with a biotinylated horse anti-mouse antibody (1:500 in PBS containing 0.5% Triton X-100, Vector Laboratories, #BA-2001-.5), followed by three PBS washes ...
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bioRxiv - Pathology 2024Quote: ... followed by incubation with biotinylated secondary antibodies (anti-rabbit or anti-rat, 1:200) and avidin-biotin complex (ABC, Vector Laboratories; CA, USA). Signal was detected using 3,3-diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2024Quote: ... or anti- tyrosine hydroxylase antibody (Pel-Freez, 40101, 1:1000) using avidin-biotin complex (ABC) system (Vectastain ABC Elite Kit, Vector Laboratories, Burlingame, CA). The immunocomplexes were visualized with the chromogen 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2024Quote: ... using a primary concentration of 1:200 and a pre-dilute secondary anti-Rb horseradish peroxidase (HRP) polymer (Vector Laboratories, Newark, California, USA) developed using 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2024Quote: ... the brain of mice surviving infection with PruΔMYR1::LucHA::mCherry tachyzoites were homogenised in 1 mL PBS and 300 µL of the sample were stained with FITC-conjugated Dolichos Biflorus Agglutinin (DBA; 1: 200; Vector Laboratories #RL-1031) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed in PBS 1X and incubated for 45 minutes with goat anti-Rabbit IgG secondary biotinylated antibody (1:500, Vector Laboratories, BA-1000) followed by an incubation with Cy3-conjugated streptavidin (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... at a concentration of 1:200 and a fluorescent-conjugated lectin (WFA) which binds carbohydrate structures and excites at 495 nm (Vector Laboratories, cat. # 1351) at a concentration of 2 ng/mL in blocking solution (2 hr ...