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Citations for Vector Labs :
1651 - 1700 of 1955 citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Samples were washed 3 times for 10 minutes each in 1 mL of 1X PBST (0.2% Tween 20 in 1x PBS) and incubated in Vectashield with DAPI (Vector Laboratories) for at least 1 hour before mounting.
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bioRxiv - Neuroscience 2023Quote: ... After incubation for 120min at room temperature in a horse anti-rabbit secondary antibody (1/200; BA-1100, Vector laboratories), sections were processed with avidin-biotin-peroxidase complex (1/200 ...
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bioRxiv - Physiology 2023Quote: ... the sections were incubated with biotinylated Sambucus Nigra Lectin (SNA) in 1% BSA/PBX at pH 6.8 (ZF0207; Vector Laboratories) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... After an hour of cool-down slides were washed with PBS and incubated with blocking buffer (1% BSA, 5% FBS, 0.01 % Triton-X 100 in 1x PBS) followed by Avidin/Biotin blocking (Vector laboratories). Slides were incubated overnight at 4°C with primary antibodies (see Supplementary Table 4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were rinsed and incubated for 30 minutes with α-rabbit IgG secondary antibody (1:1000; Vector labs, PK-4001) diluted in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation of the sections in biotinylated anti-rabbit/mouse secondary antibody (1:1,000 dilution, Vector Laboratories, Burlingame, CA) and then in avidin-biotin-peroxidase complex (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... and Ki67 immunostaining signals were further amplified by tyramide-FITC or tyramide-Cy3 (1:2,000, Perkin-Elmer, Wellesley, MA) after avidin-biotin complex incubation (Elite ABC kit, Vector Laboratories). Immunostained sections were counterstained with DAPI (1 μg /ml ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was washed by PBS-TX (0.3% Triton X-100) and then incubated with 1:1000 dilution of biotinylated anti-mouse antibody (Vector Labs) with agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary detection was performed with: Streptavidin DyLight 488 nm (1:200 dilution, Vector Laboratories, Burlingame, CA, catalog no. SA-5488), Goat anti–Rat Alexa 546 nm (catalog no ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# 111-035-144] and Peroxidase-conjugated horse anti-mouse IgG (H+L) antibody [(1:5,000) (Vector laboratories, PI-2000)].
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in PBS-T and incubated in blocking solution containing DyLight 488 Streptavidin (1:250, Vector Laboratories) for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... followed by 1 h incubation with ImmPRESS VR anti- rabbit IgG HRP polymer detection kit (MP-6401-15; Vector Laboratories). Chromogenic detection in situ was performed using ImmPACT DAB EqV peroxidase substrate solution (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated with the biotinylated secondary goat anti-rabbit antibody (1:200 BA-1000, Vector Laboratories) 2 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were detected using a biotinylated goat anti-rabbit IgG secondary antibody (Vector Laboratories, USA; #BA-1000, 1:200), followed by a tertiary antibody (Vector Laboratories ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:1000 in 5% nonfat dry milk/TBS-T or goat anti-rabbit IgG-HRP (Vector Laboratories #PI-1000) diluted 1:20,000 in 5% nonfat dry milk/TBS-T – for 1 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Cells treated with extensive washes with PBS with 1% BSA prior to mounting in anti-fade media (Vector Laboratories, Inc.). The samples were then imaged using confocal microscopy with a LSM 710 laser scanning confocal microscope (Carl Zeiss ...
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bioRxiv - Bioengineering 2024Quote: ... with 0.1% Triton-X and then incubated in the same buffer containing lectin-fluorescein (0.5 mg/ml, FL-1171, Vector Laboratories), overnight for spinal cords and two days for brains ...
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bioRxiv - Neuroscience 2024Quote: ... β-amyloid was detected using thiazine red (1 µM, Cat# 2150-33-6, Chemsavers) associated with DAPI (0.5 µM, Cat# 1485, Vector lab) and a mouse monoclonal antibody against VSM α-smooth muscle actin (Cat# ABT1487 ...
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bioRxiv - Immunology 2024Quote: ... membranes were washed and incubated with an HRP-conjugated anti-rabbit secondary antibody (PI-1000; 1:40,000 dilution; Vector Laboratories). Protein signals were detected on film and quantified using ImageJ software ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol before samples were stained with DAPI (1 µg/ml, 5 min) and mounted in Vectashield (Vector Laboratories). Images were collected with a Leica SP5 II confocal microscope using LAS-AF software (v2.7.3.9723) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein levels were determined by incubating the membrane with ClpL-specific antibodies (1:10,000 in TBS-T + 3% (w/v) bovine serum albumin) and an anti-rabbit alkaline phosphatase conjugate (Vector Laboratories) as the secondary antibody (1:20,000) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 2 hours in 0.1 M PBS and 0.4% Triton X-100 containing biotinylated rabbit Anti-Rat IgG antibody diluted to 1:1000 (Vector Laboratories) and washed 3*10 minutes with 0.1 M PBS and 0.4% Triton X-100 ...
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bioRxiv - Molecular Biology 2024Quote: ... Alexa Fluor donkey anti-goat) or conjugated to horseradish peroxidase (anti-rabbit and anti-mouse IgG [H+L], 1:8.000; Vector Laboratories).
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bioRxiv - Cancer Biology 2024Quote: ... was run at 1:200 dilution with EDTA antigen retrieval and Goat Anti-Rat IgG secondary antibody from Vector Labs catalog number PI-9401-.5 ...
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bioRxiv - Bioengineering 2024Quote: ... Wells were briefly washed in PBS followed by 1 h incubation with animal free blocking media (Vector Labs SP-5035). Following blocking ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were briefly washed in PBS followed by 1 h incubation with animal free blocking media (Vector Labs SP-5035). Sections were once again washed in PBS before incubation with primary antibody overnight at 4C.
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibody conjugates used for western blotting were HRP-linked horse anti-mouse IgG (Vector Laboratories, PI-2000, 1:10,000), goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Genetics 2024Quote: ... samples were washed three times in 2x SSC + 1 mM EDTA + 0.1% Tween-20 for 20 minutes each and mounted in VECTASHIELD with DAPI (Vector Labs). The following satellite DNA probes were used ...
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bioRxiv - Microbiology 2020Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the N 1C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:3000 in PBS 1X and then mounted with Vectashield antifade medium (cat. H-1000 - Vector Laboratories, Burlingame, CA, USA) and analysed immediately or stored at +4°C until ready for confocal image analysis.
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour at room temperature in 3% normal donkey serum (NDS, Vector Laboratories, Inc., Burlingame, CA, USA) and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Microbiology 2022Quote: ... fixed in 4% paraformaldehyde for 15 minutes then stained with a 1:500 dilution of dolichos bifluorus agglutinin conjugated to FITC in PBS (Vector Labs). FITC-positive cysts were confirmed by fluorescence and morphology under 20x magnification and the total cyst burden per brain was back-calculated.
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bioRxiv - Neuroscience 2021Quote: ... or Alexa 647 donkey anti-mouse (1:200, Thermo A31571) and then mounted with the VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratory).
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in biotinylated secondary antibody diluted 1:500 in block (biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories). Sections were washed in TBS and then incubated for 1 hour at RT in Vectastain kit components A and B ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the samples were washed with 1× TBS followed by incubation for 1 h at room temperature with a biotinylated secondary antibody (PK-6102, horse serum, mouse IgG or PK-6101, goat serum rabbit IgG; 1:3000 dilution; Vector Laboratories) in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval was performed in the Biocare Medical LLC decloaking chamber (NC0436641) for 1 hour with citrate-based antigen retrieval buffer (H-3300, Vector Laboratories) and washed with PBS ...
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bioRxiv - Bioengineering 2020Quote: ... sections were washed in PBS and then incubated with a biotinylated goat anti-rabbit secondary antibody (1:200, Vector Laboratories, BA1000) for one hour at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Three drops of normal goat serum in 10ml PBS was used as blocking buffer for 1 hr at room temperature before primary antibodies incubation (Vectastatin ABC Kit; Vector Laboratories). Slides were then incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... To isolate OPC the single cell suspension was sequentially panned on two panning plates coated with anti-BSL 1 Griffonia simplificonia lectin (#L-1100, Vector Labs) and then incubated on a plate coated with anti-CD140a (#135902 ...
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bioRxiv - Neuroscience 2021Quote: Sections used for morphological analyses in Figure 1C were further incubated in a solution of conjugated avidin-biotin horseradish peroxidase (ABC; 1:300; Vector Labs) in TBS (pH = 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200, Vector Laboratories, Burlingame, CA, USA), and then incubated for 1h in an avidin-biotin peroxidase complex (Vectastain ABC Elite PK6100 ...
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bioRxiv - Neuroscience 2020Quote: ... Corresponding secondary antibodies (1:500 goat anti-rabbit/goat anti-mouse biotinylated secondary antibody (Vector Laboratories, BA-1000 and BA-9200) were added ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were then washed with PBS and mounted using DAPI (1 μg/ml) or VECTASHIELD with DAPI Mounting Medium (Vector Laboratories). Images were captured using a Zeiss 780/880 confocal microscope.
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bioRxiv - Neuroscience 2020Quote: ... Sections were again rinsed in PB and then incubated overnight in biotinylated donkey anti-sheep IgG in PB (1:200, Vector Labs), followed by overnight incubation in streptavidin-conjugated Alexa 488 in PB (1:800 ...
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bioRxiv - Microbiology 2020Quote: ... followed by a 1h incubation of the following primary antibodies diluted in blocking buffer: biotinylated-dolichos biflorus agglutinin (DBA) [1:500] (Vector Laboratories), rabbit-anti SRS9 [1:1000] and mouse-anti SAG1 [1:1000](kindly provided by John Boothroyd ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed 3 times in PBS and the secondary antibody (biotinylated horse anti-rabbit; 1:250, Vector Laboratories, Burlingame, CA) was added and slides were incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... were applied for 1 h at 37°C prior to mounting onto slides using mounting media containing DAPI (Vectashield, Vector Labs). Images were obtained on a Leica SP5 confocal microscope or a Nexcelom Celigo as described in individual figure legends.
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bioRxiv - Neuroscience 2020Quote: ... 30 min in biotinylated goat anti-mouse or anti-rabbit IgG (1:200, PA-920 or BA-1000; Vector Laboratories, USA); 30 min avidin-biotin complex solution (PK-7100 ...
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bioRxiv - Microbiology 2020Quote: ... The homogenate was blocked using 3% bovine serum albumin (BSA) in 0.2% Triton X-100 before staining with 1:250 rhodamine-conjugated Dolichos biflorus lectin (Vector Laboratories Inc.) to visualize the cyst wall ...