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Citations for Vector Labs :
1551 - 1600 of 1768 citations for Anti EFHC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 2 h (at RT) with a secondary biotinylated antibody followed by an avidin-biotin-peroxidase complex (ABC; Vector Laboratories, Peterborough, UK) for 1 hour in PBS ...
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bioRxiv - Immunology 2023Quote: ... The secondary antibodies were washed off with above-mentioned washing buffer for 15 min and finally slides were mounted with DAPI (Vector Laboratories, Burlingame, USA). The stained cells were examined under a Zeiss LSM 780 fluorescence microscope (Carl Zeiss MicroImaging ...
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bioRxiv - Neuroscience 2023Quote: ... labeled with rabbit anti-ionized calcium-binding adapter molecule (Iba)-1 antibody (Wako Chemicals, Neuss, Germany) and VectaStain Elite ABC-HRP kit (Cat.-No.: PK-6100, Vector Laboratories, Burlingame, USA), and visualized with diaminobenzidine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies with their respective concentrations were as follows Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 (1:25, Vector Laboratories, #FL-1201), mouse monoclonal anti-3G5 IgM (1:10 ...
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bioRxiv - Neuroscience 2024Quote: ... The next day sections were incubated at room temperature for one hour with a biotinylated secondary antibody (1:200, Vector Laboratories, Burlingame, CA), washed briefly in PBS ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.6 at 4°C overnight and goat anti-mouse IgG (#BA 9200, Vector Laboratories, Burlingame, CA) diluted 1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted on gelatin subbed slides and coverslipped with VectaShield anti-fade mounting medium (Vector Labs).
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-mouse secondary using the ImmPACT DAB-EqV HRP (brown) substrate kit (SK4103, Vector Laboratories, UK); signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100 ...
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bioRxiv - Pathology 2021Quote: ... Sections were washed with PBS-Triton 0.1% and incubated in ImPRESS REAGENT anti-rabbit IgG (Vector Laboratories) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and processed in the same manner with biotinylated anti-mouse secondary (1:600, Vector Laboratories BA-2000), but nickel was not included in the second DAB reaction to produce a brown reaction product instead of black ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were then incubated with goat anti-rabbit or mouse IgG horseradish peroxidase (1:10,000; Vector Laboratories) for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... tissue sections were stained with the ImmPRESS HRP anti-Rabbit Detection Kit (Vector Laboratories MP-7401-50) and ImmPACT DAB Peroxidase Substrate (Vector Laboratories SK-4105 ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were incubated in ImmPRESS® HRP Goat Anti-Rabbit IgG Polymer Detection Kit (Vector Laboratories) for 30 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... ImmPRESS polymer anti-rabbit IgG secondary reagent (Vector Labs, San Francisco, CA, USA t#MP-7401-15) was used for secondary staining of CC3 and pSTAT3 stained tissues and anti-rabbit secondary (Dako #K4003 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections and coverslips were mounted by vector anti-fading mounting medium (Vector Laboratories, Burlingame, CA, USA). The fluorescent intensity was imaged using Leica SP8 confocal microscopy at 40 × magnification ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted on gelatin subbed slides and coverslipped with VectaShield anti-fade mounting medium (Vector Labs). 10X images were taken on a Zeiss AxioImager microscope to co-labeling of Th and dsRed.
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted on gelatin subbed slides and coverslipped with VectaShield anti-fade mounting medium (Vector Labs).
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bioRxiv - Neuroscience 2020Quote: ... Sections incubated in biotinylated goat anti-rabbit IgG (1:500 BA-1000; Vector Laboratories, Burlingame, CA, USA) for 2 hours at room temperature (RT) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a 60 minute incubation with biotinylated horse anti-mouse IgG (Vector labs, cat# MKB-22258) or biotinylated goat anti-rabbit (Vector Labs ...
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bioRxiv - Neuroscience 2022Quote: ... Brain slices were mounted to slides and cover slipped with Vectashield anti-fade mounting medium (Vector Labs). A Zeiss Apotome.2 microscope was used to image brain slices for GFP (Zeiss Filter set 38 ...
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bioRxiv - Cancer Biology 2022Quote: ... The coverslips were then adhered to glass slides using an anti-fade Vectashield mounting medium (Vector Laboratories). Images were captured with a Carl Zeiss Axio Observer Z1 inverted fluorescence microscope fitted with an Apotome device ...
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bioRxiv - Biochemistry 2019Quote: ... Immunohistochemical staining was performed with the ImmPRESS HRP anti-rabbit kit according to manufacturer’s instructions (Vector Labs), followed by incubation with DAB substrate (Vector Labs) ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemical staining was carried out with ImmPRESS HRP Horse Anti-Rabbit IgG Polymer Kit (Vector Laboratories, Inc.), together with the Vector VIP Substrate Kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... before a 4-hour incubation in 1:500 biotinylated Goat anti-Rabbit IgG (H + L) (Vector Labs) secondary in 2.5% NGS in 1x TBS-0.3% Triton at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... slides were incubated with ImmPress horseradish-peroxidase-labeled horse anti-rabbit detection kit (Vector Laboratories #MP-7401) at 22 °C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... the cells were placed on panning plates coated with goat anti-rat IgG (Vector Laboratories, #BA-9400) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... then conjugated with the secondary (Goat anti-Rabbit ImmPRESS HRP, #MP-7451 Vector Labs, Newark, CA, USA) for an additional 30 min ...
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bioRxiv - Biophysics 2023Quote: ... a nitrocellulose coated sample chamber was incubated with 10 ng/μL anti-digoxygenin (Vector Labs MB-7000) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: ... The slides were rinsed with PBS and then incubated with anti-IgG polymer detection solution (Vector Labs) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were then incubated over night at 4°C on rotating wheel (or 2 h at RT) with a secondary antibody coupled to a fluorescent dye (DyLight® 549 (VECTOR lab # DI 1549) (1:400 ...
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bioRxiv - Neuroscience 2019Quote: ... The sections of cerebellum were incubated with monoclonal mouse antibodies against HuCD (ICN, Biomedicals, United States) (1:300) and glutamine synthetase (Vector Laboratories Burlingame, United States) (1:300 ...
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... then washed 3 times in TBS before applying secondary antibody for 30 minutes at room temperature (Cat# MP-7451, Vector Laboratories, Burlingame, CA, USA). Slides were washed twice for 5 minutes each before applying DAB for 2 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for two hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... After washing in 0.1% Triton-X-100 in TBS the sections were incubated for one hour at room temperature with Streptavidin Dy Light Alexa antibody (1:500; Vector Labs, Cat. # SA-5488) in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for monoclonal antibodies; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for polyclonal antisera and monoclonal antibodies, respectively; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... the slides were incubated with a biotin-conjugated secondary antibody for 1 h and with VECTASTAIN ABC Reagent (Cat. #PK- 4000; Vector Laboratories, Burlingame, CA, USA) for 30 min at 20°C ...
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bioRxiv - Neuroscience 2019Quote: ... All stainings were visualized using respective secondary antibodies conjugated with HRP or ABC-system (Vectastain Elite ABC HRP Kit, Vector Laboratories Cat# PK-6100) and 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2023Quote: ... Detection of antibody signal was revealed with 3,3’-diaminobenzidine (DAB) peroxidase substrate (DAB Substrate Kite, Peroxidase with Nickel, Vector Labs #SK-4100, Burlingame, CA).
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were incubated with primary antibody overnight at 4°C then exposed to biotinylated anti-rabbit secondary antibody (Vector Labs, BA-1000, 1:400 dilution, 30Dmin at room temperature followed by avidin-biotin complex formation (Vector Laboratories, # PK-6100), staining with DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2023Quote: ... Slides were then washed with PBS and incubated at room temperature for one hour with appropriate secondary antibodies (Vector Laboratories MP- 7401, MP-7402). Slides were developed with diaminobenzidine substrate (Vector Laboratories SK-4100 ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibody followed by 3,3’-Diaminobenzidine (DAB) staining using the ImmPRESS™ Excel Amplified HRP peroxidase Polymer Staining Kit (Vector Laboratories MP-7602-15), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were probed with fluorochrome-conjugated secondary antibodies for 1 hour at room temperature and mounted using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). Images were taken with the LSM 880 confocal microscope (Zeiss).
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... The following day, the sections were incubated with secondary antibodies (1 h, room temperature) and mounted with Vectashield® Mounting Medium (Vector Laboratories, #H-1000). Image acquisition was performed using a Leica SP8 confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed in PBS two times for 10 minutes and then primary antibodies were applied overnight in 10% normal horse serum (Vector Labs, #S-2000-20), 0.5% Triton-X-10 (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were washed with TBS-T for 5 minutes three times on a shaker at room temperature and incubated with HRP-conjugated secondary antibody (1:8000 in TBS-T; Vector Laboratories, PI-2000-1) for one hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with the appropriate biotinylated secondary antibody for 30 minutes followed by avidin-biotin complex and visualization achieved using DAB (all reagents: Vector Labs, Burlingame, CA, USA). Sections were rinsed and dehydrated in graded alcohols and cleared in xylenes before being coverslipped ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min, a first antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, Rabbit α-Phal-L 1:8000 (Vector Laboratories, AS-2300, RRID: AB_2313686), Mouse α-PV 1:4000 (Swant ...