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Citations for Vector Labs :
1401 - 1450 of 1768 citations for Anti EFHC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... under 50% relative humidity at a surface temperature of 12°C with lectins and antibodies that were purchased from Vector Labs, R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... Cover slides were incubated with Alexa labeled secondary antibodies and mounted in VECTASHIELD Antifade Mounting Medium with DAPI (Vector Labs, USA). The cells were finally examined by fluorescence microscopy (Olympus America Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... A biotinylated goat anti-rabbit IgG was applied to detect rabbit primary antibodies and complexes were visualized using the R.T.U Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA). Mouse primary antibodies were visualized applying the VECTASTAIN® ABC HRP Kit (Peroxidase ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with secondary antibodies for 1 h at RT then washed further in PBS and mounted in Vectashield containing DAPI (Vector Laboratories). Primary antibodies used were ...
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bioRxiv - Cancer Biology 2020Quote: ... after washing sections were incubated with biotinylated secondary antibody at room temperature for 1 hour followed by incubation with ABC (Avidin Biotin complex) (Vector Labs) for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissues were incubated in biotinylated secondary antibodies for 30 minutes at room temperature and treated with VECTASTAIN Elite ABC Reagent (Vector Laboratories) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with primary antibody for overnight and secondary antibody for 20 minutes and then the staining was amplified using avidin-biotin-peroxidase conjugate (ABC elite; Vector Laboratories) and visualized using 3 ...
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bioRxiv - Immunology 2020Quote: ... Detection of primary antibody was performed using the Super-sensitive–Polymer HRP system (Biogenex) and staining visualized using purple chromogen VIP (Vector Labs) and hematoxylin as a nuclear counterstain ...
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bioRxiv - Microbiology 2022Quote: ... for 15 min at room temperature and immunostained using the N protein 1C7C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS-T and then incubated for 2 h at RT with biotinylated secondary antibody (1:200, Vector Laboratories). After subsequent washes with PBS-T ...
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bioRxiv - Physiology 2022Quote: ... # AMR-025) followed by biotinylated horseradish peroxidase-conjugated secondary antibody incubation and detection with diaminobenzidine (ABC kit, Vector Labs, Burlingame, USA). For isotype control ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Neuroscience 2024Quote: ... It was reacted with a second antibody and the positive brown signal generated with DAB peroxidase-based methodology (Vector Laboratories, SK4100). Sections were counterstained with haematoxylin alone ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by a 10 min incubation with secondary antibody (either ImmPress-mouse HRP, MP-7402, or ImmPress-rabbit HRP, MP-7401, Vector Laboratories) and fluorescent labelling for 10 min using tyramide signal amplification with Opal dyes in the sequence of 690 ...
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bioRxiv - Cancer Biology 2024Quote: ... Incubation with biotinylated antibodies slides followed by modified Elite avidin-biotin peroxidase (ABC) method (Vector Laboratories, Burlingame, CA, USA; pk-6100) for 30 minutes (RT) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with DPBS and incubated with secondary antibody (Jackson) 1:100 in DPBS for 1 hour and mounted with DAPI (Vector Labs).
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bioRxiv - Immunology 2024Quote: ... Successful attachment of azide groups to antibodies was confirmed by a test conjugation with DBCO-AZDye (Click Chemistry Tools/Vector Laboratories) followed by detection of fluor-conjugated antibodies using UltraComp eBeads (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... We then used flow cytometry to confirm higher expression of Tn on the AT3 cell surfaces using the ReBaG6 antibody (generously provided by Richard Cummings at Harvard University) (Supplementary Fig. 1A) (57) and VVL lectin (Vector Laboratories) (Supplementary Fig ...
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bioRxiv - Neuroscience 2023Quote: ... the neuronal cultures were washed with PBS three times and incubated in Alexa Fluor conjugated secondary antibodies and DAPI (Vector Labs) diluted in a blocking buffer containing 10% NGS for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were washed for three times in TBS and incubated in 10% normal goat serum in TBS-T with a biotinylated secondary antibody from goat against rabbit IgG (1:1000, Vector Laboratories) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... using Dako Link48 plus as a service with the primary antibodies described in the Key Resources Table and species-appropriate ImmPRESS Polymer Detection Kit (Vector Laboratories) and developed with 3,3’-Diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2023Quote: ... This procedure was modified for mouse primary antibodies for use with the Mouse on Mouse Immunodetection Kit (BMK-2202; Vector Laboratories) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation with the biotinylated secondary antibody (1:150, Vector in 5% NGS in PBST) for 1 h was followed by the Vector ABC Kit (Vector Laboratories, solution A and solution B in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in 0.2% Triton X-100 PBS and incubated in secondary antibody solution for 1 hour before amplification of the signal by incubation in Vectastain ABC kit (1:400, Vector Laboratories) for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... free-floating sections were rinsed and bound antibodies were visualized by the Avidin-Biotin complex (ABC) amplification system (Vectastain ABC kit, Vector Laboratories) using 3,3′-diaminobenzidine tetrahydrochloride (DAB Substrate Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Development of the biotinilated antibody signal was done by 1 h incubation with the Avidin/Biotin-HRP complex (Elite Vectastain ABComplex Kit, Vector Laboratories) following 1 h incubation with Streptavidin-647 (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS and blocked and stained using appropriate primary antibodies and reagents from the Vectastain Elite ABC kit (Vector Labs). Diaminobenzidine exposure was titrated to optimal contrast ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromogen amplification and detection of antibodies was performed using the Vectastain Elite ABC-HRP Kit and DAB Peroxidase Substrate Kit (Vector Labs). Antigen unmasking was performed with a citrate buffer pH 6.0 by a modified microwave retrieval method or boiling ...
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bioRxiv - Physiology 2023Quote: ... of paraffin-embedded tissues using specific commercially available primary antibodies (Supplementary Table S1) and AlexaFluor 488 and 568-conjugated F(ab’) secondary antibody (Molecular Probe) or VECTASTAIN ABC kit (Vector lab). Cell-specific CD68 positive and total cell numbers were counted in the area of 0.07244 mm2 ...
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bioRxiv - Microbiology 2023Quote: ... the secondary antibody was applied for 30 min and rinsed (twice) in TBST before reaction with Vector ImmPACT DAB (Vector Labs) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with Alexa Flour-conjugated secondary antibody for 1 hr at room temperature and mounted in Vectashield mounting medium containing DAPI (Vector Laboratories). Nikon A1 Confocal microscope (Nikon ...
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bioRxiv - Physiology 2024Quote: ... was incubated at 4°C overnight and a biotinylated secondary antibody followed by streptavidin-DyLight 594 (BA1000 and SA-5594, Vector Laboratories) were used for cardiomyocyte identification ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated with 594 (or 488) labeled secondary antibodies (1:200) and DAPI-containing mounting solution VECTASHIELD (Vector Laboratories). The slices were visualized by using a Nikon inverted microscope Eclipse Ti-U equipped with a digital camera ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated for 90 minutes with appropriate secondary pre-diluted antibody from the Vectastain ABC kit (Vector Laboratories Ltd, UK).
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bioRxiv - Neuroscience 2024Quote: ... was followed by incubation with the primary antibody (Table S2) at 4°C for 24 or 48h in 2% (vol/vol) serum and with the corresponding biotinylated antibody (Vector Laboratories) or alkaline-phosphatase antibody (ab6722 ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... horse anti-rabbit AP (blue) using a BCIP/NBT substrate kit (SK5400, Vector Laboratories, UK); rabbit anti-mouse secondary using the ImmPACT DAB-EqV HRP (brown ...
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bioRxiv - Genetics 2020Quote: ... Biotin- and digoxygenin-labeled probes were detected by anti-biotin fluorescein (Vector Laboratories, Burlingame, California) and anti-digoxygenin rhodamine (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with ImmPRESS goat anti-rabbit IgG (MP-7451, Vector Laboratories, Burlingame, CA) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 60-minutes incubation with biotinylated goat anti-rabbit IgG (Vector labs, cat#PK6101) at 1:200 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... at room temperature for 45 min or a biotin-labeled anti-rat IgG (Vector Laboratories) at 37°C for 30 min followed by HRP- labeled streptavidin (Dako ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 60-minutes incubation with biotinylated goat anti-rabbit IgG (Vector labs, cat#PK6101) at 1:200 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 60-minutes incubation with biotinylated goat anti-rabbit IgG (Vector labs, cat#PK6101) at 1:200 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 60 minutes incubation with biotinylated goat anti-rabbit IgG (Vector labs, cat#:PK6101) in 5.75ug/ml ...
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bioRxiv - Microbiology 2020Quote: ... and mounted with anti-fade mounting medium with DAPI (Vectashield, Co#: H-1200, Vector Laboratories). For intracellular staining of Env with 2G12 ...
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bioRxiv - Bioengineering 2020Quote: ... The coverslips were mounted onto glass slides with anti-fade mounting media (Vector Laboratories, Inc.). Fixed cells were imaged using a Fluoview FV10i confocal microscope (Olympus ...
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bioRxiv - Microbiology 2022Quote: ... at a dilution of 1:30,000 followed by goat anti-rabbit secondary (Vector Labs #BA1000). Signal was developed with ImmPact NovaRED HRP substrate (Vector Labs # SK-4805).
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bioRxiv - Neuroscience 2022Quote: ... ImmPRESS®-AP Horse Anti-Mouse IgG Polymer Kit (alkaline phosphatase, MP5402, Vector laboratory, CA), and Vector® Blue (alkaline phosphatase substrate ...