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Citations for Vector Labs :
1501 - 1550 of 2431 citations for Mouse Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... while ImmPACT* AMEC Red* Substrate Kit was from Vector Laboratories (Shanghai, China). All the mice sera were diluted 100-fold using a high-salt sample buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Labeling was detected using the Click-&-Go® detection kit (Vector Laboratories) following the standardized protocol and analyzed by flow cytometry.
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bioRxiv - Cancer Biology 2024Quote: ... Chromogenic detection was performed with VECTASTAIN Elite ABC-HRP kit (Vector Laboratories) followed by counterstaining with hematoxylin ...
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bioRxiv - Neuroscience 2024Quote: ... and avidin-biotin-peroxidase complex (1:300, ABC Elite Kit, Vector Laboratories) in PBS for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... treated with avidin-biotin–horseradish peroxidase complex (ABC Elite kit, Vector Laboratories). The brightfield signal was developed using a 3,3′-diaminobenzidine kit (Vector Laboratories).
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-diaminobenzidine reaction was performed using the ABC Kit (Vector Laboratories), using biotin-labeled goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2024Quote: ... For DAB staining (Vectastain ABC-Elite Standard kit, Vector Laboratories PK-6100), slices were fixed in a solution containing 2.5% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were then stained with 4’,6-diamidino-2-phenylindole (DAPI) and cells were mounted with Vectashield (Vector Labs, www.vectorlabs.com, Burlingame, CA). Myoblast fusion was quantified by counting myonuclei in myotubes ...
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bioRxiv - Genetics 2021Quote: ... Stained embryos were washed in PBST and mounted onto glass slides using Vectashield® with DAPI (4’,6-diamidino-2-phenylindole, Vector labs). Embryos were imaged using Ti2E Spinning Disc confocal microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... then mounted on slides in hard set mounting medium containing 4,6-diamidino-2-phenylindole (DAPI) (Vectashield, H-1500, Vector Laboratories, Burlingame, CA). A confocal system (TCS SP8 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then mounted in 4’,6-diamidino-2-phenylindole (DAPI)-containing anti-fade solution (VECTASHIELD anti-fade mounting medium, H-1200, Vector Laboratories, CA) and imaged with Zeiss Axio Scan Z1 slide scanner fluorescence microscope (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Immunology 2021Quote: ... Slides were mounted and nuclei were stained with 4’,6-Diamidino-2-Phenylindole (DAPI) fluorescent mounting medium (VECTASHIELD®, Vector laboratories Inc). Images were taken using confocal laser scanning microscope LEICA TCS SP8 (LEICA microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... and cover slipped with a mountant containing the nuclear stain 4’,6-diamidino-2-phenylinodole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA). Slides were stored in the dark at −20°C until imaging.
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bioRxiv - Developmental Biology 2020Quote: ... A small aliquot of embryo pellet (2 μL) was taken right before freezing and mixed with 10 μL VECTASHIELD® Antifade Mounting Medium with DAPI (Vector laboratories) and immediately frozen in dry ice ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The slides were then coverslipped with antifade mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Images were captured using a Nikon C2+ laser scanning confocal head mounted on an Eclipse Ti2 inverted microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted and coverslips were applied with Vectashield mounting medium containing DAPI (4’-6-diamidino-2-phenylinodole; Vector Laboratories, Burlingame, CA). Sections were visualized on a Zeiss AxioImager M2 microscope (Zeiss ...
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bioRxiv - Plant Biology 2022Quote: ... the slides were dehydrated in 70-90-100% ethanol series for 3 min each and counterstained with 10 µg/ml 4’,6-diamidino-2-phenylindoline (DAPI) in Vectashield antifade medium (Vector Laboratories, USA). For immunodetection of microtubules ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Slices were then washed overnight at 4 °C in a biotinylated Wisteria Floribunda Agglutinin (WFA, 1:2000, Vector Laboratories, B-1355-2) followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Immunology 2023Quote: ... we used the lectin from Datura stramonium at a staining concentration of 10 µg/ mL (Biotinylated DSL, Vector laboratories, B-1185-2). 2 × 105 cells per condition were collected and rinsed three times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS and incubated in biotinylated goat anti-rabbit (Vector labs, 1:500 in PBS-TX for 2 hrs). Sections were washed in PBS and then incubated in avidin-conjugated peroxidase (Vector labs ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody labeling was amplified with biotinylated goat anti-rabbit IgG for 2 hr (1:500 in PBS, BA-1000, RRID: AB_2313606; Vector Laboratories; Burlingame, CA) followed by Vectastain ABC Solution for 1 hr (1:500 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Then a secondary biotinylated goat anti-rabbit antibody was applied in PBST / 2% NGS (1:200; Vectastain, Vector Laboratories, Burlingame, CA, USA) (75 min ...
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bioRxiv - Neuroscience 2023Quote: ... 30-80 MΩ) lowered vertically and filled with a solution of potassium acetate (0.5M) and Neurobiotin (2%, Vector Laboratories, Burlingame, CA, USA). The signal was amplified and low-pass filtered (10kHz ...
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bioRxiv - Microbiology 2024Quote: ... The parasitophorous vacuoles (PV) of the parasites of were then stained with 2 μg/mL fluorescein labelled Vicia Villosa lectin (VVL; Vector Laboratories #ZG0124) in 1% BSA/PBS for 1 hour at room temperature then nuclei stained with 2 μg/mL DAPI (Merk #096M-4014V ...
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bioRxiv - Neuroscience 2019Quote: ... The sections of cerebellum were incubated with monoclonal mouse antibodies against HuCD (ICN, Biomedicals, United States) (1:300) and glutamine synthetase (Vector Laboratories Burlingame, United States) (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... retinas were washed in 0.5% TX-100 and incubated for 1.5 hours in anti-mouse biotinylated secondary antibody (1:400; Vector Laboratories; cat # BA-9200; RRID: AB_2336171). Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... secondary antibodies (goat anti-rabbit, Vector Laboratories inc., PI-1000, 1:1,000 and biotinylated horse anti-mouse, Vector Laboratories inc., PK-4002, 1:200) were added to the sections for 150 min ...
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bioRxiv - Bioengineering 2022Quote: ... primary monoclonal rat anti mouse Mac1 antibodies (AbD Serotec, Raleigh, NC, USA) were used along with secondary biotinylated goat anti-rat antibodies (Vector Laboratories, Burlingame, CA, USA). Each midbrain section was viewed at low power (10x objective) ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed three times for 10 min with PBST and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (HRP anti-rabbit [Jackson ImmunoResearch, 111-035-144] or HRP anti-mouse [Vector Laboratories, PI-2000) at 1:15,000-1:25,000 ...
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bioRxiv - Immunology 2022Quote: ... were incubated at 4°C overnight followed by washing and incubation with a secondary biotinylated goat anti-mouse IgG antibody (dilution: 1:200, Vector Laboratories, Burlingame, California, USA). For color development freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed with PBS and incubated for 1 hour at room temperature in secondary antibody solution 1:1000 anti-mouse (Vector Laboratories, Burlington, ON, Canada), 2% normal horse serum ...
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bioRxiv - Cell Biology 2021Quote: ... or goat anti-mouse IgG Alexa Fluor 647 (Thermo, A32728) were diluted 1:200 in blocking buffer and 1% normal goat serum (Vector Laboratories, S-1000-20) and incubated for 2 hours at room temperature in a dark container ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in the appropriate biotinylated secondary antibody (goat anti-rabbit, #BA-100; goat anti-mouse, Vector Labs #BA-9200;Vector Laboratories, Burlington, ON Canada) and then incubated with avidin-biotin complex (Vectastain ABC kit ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 1 hour at room temperature in biotinylated anti-mouse secondary antibody at 1:500 dilution (Vector Laboratories; cat # BA-9200; RRID: AB_2336171), followed by incubation in ABC Media 1:800 (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then stained with secondary anti-mouse IgG and anti-rabbit IgG HRP-conjugated antibodies for 30 min at RT (Vector Laboratories, MP-7500-50). After washing twice in washing buffer for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... The cells were incubated with an anti-NS3 (clone 578) antibody (54) for 30 min and then with a biotin-conjugated anti-mouse IgG secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His tag and S1 proteins were purified using Ni-NTA resin (Thermofischer, cat.no. 88221) and Agarose bound Galanthus Nivalis Lectin (GNL) (Vector Labs, cat. no. AL-1243-5) respectively ...
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... Incubation in the primary antibodies was followed by 2-hour incubation in anti-rabbit secondary biotinylated antibodies (1:200, Vector Laboratories, Burlingame, CA), and then in avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Neuroscience 2019Quote: ... TC neurons in 250 micron brain slices or RGCs in flat-mount retinas were loaded by passive diffusion with 2% neurobiotin (Vector Labs, Burlingame, CA) and/or 100 μM CF568 dye (Biotium ...
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bioRxiv - Genetics 2021Quote: ... the gonads were again washed three times with PBSTw and resuspended in 1 drop of Vectashield containing 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA). The gonad was finally mounted on pads of 5% agarose in water on microscope slides and covered with a cover glass (Sulston and Horvitz ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2021Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2021Quote: ... The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories, CA, USA). Cell counting was done manually on brain sections containing the mPFC ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 μl/ml avidin and 6 μl/ml biotin; 2 hours at room temperature; Vector Laboratories, CA, USA), washed in PB ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated in the second antibody solution for 1 h at 37° C and subsequently mounted in Vectashield mounting medium containing 4′,6-diamidin-2-phenylindol (DAPI, Vector Laboratories, Burlingame, California) in a manner that conserves the 3D structure of the specimen [42] ...