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Citations for Vector Labs :
1301 - 1350 of 2431 citations for Mouse Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in Vector TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories) for 3 minutes at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Staining was then revealed with a DAB substrate kit (Vector Laboratories) and tissue sections were counterstained with hematoxylin (see Supplemental table 2 for secondary antibody references).
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bioRxiv - Cancer Biology 2023Quote: ... an additional step of biotin blocking kit (Vector Labs -SP-2001) was performed ...
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bioRxiv - Neuroscience 2023Quote: ... and developed using Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA) followed with 3,3’- diaminobenzidine (DAB ...
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bioRxiv - Immunology 2024Quote: ... CD68+ area was visualized using a Vectastain ABC kit (Vector Laboratories). Sections were then counterstained with hematoxylin ...
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bioRxiv - Microbiology 2024Quote: ... after which a Vectashield quenching kit (Vector Laboratories, SP-8400-15) was utilized as per the company’s recommendations to limit background fluorescence ...
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bioRxiv - Genetics 2024Quote: ... autofluorescence was quenched using Trueview autofluorescence quenching kit (Vector Labs #SP8500) and mounted with Vectashield containing DAPI (Vector Labs #30326) ...
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bioRxiv - Cell Biology 2024Quote: ... kit was used after the peroxidase block (Vector Labs, MP-2400). Antibodies used for IHC are listed in Supplementary Table 10 ...
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bioRxiv - Cancer Biology 2024Quote: The Alcian Blue (pH 2.5) Stain Kit (Vector Laboratories #H-3501) was used for this protocol ...
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bioRxiv - Neuroscience 2024Quote: ... A Vector Blue alkaline phosphatase substrate kit (Vector Laboratories: SK-5300) was used to develop HBβ signals ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal development was performed by ImmPRESS Polymer Detection Kits (Vector Laboratories, #MP-7401-50 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with 3% NDS-PB-TX and first incubated in biotinylated secondary anti-mouse antibody (1:200, Vector Labs, CAMK-IIα amplification) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was washed 3 times for 5 minutes in 0.1 M PB and placed in goat anti-rabbit OR anti-mouse biotinylated secondary antibodies respectively (1:500, BP-9200/9100; Vector Labs, CA) for 6 hours ...
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bioRxiv - Biochemistry 2021Quote: ... or mouse anti-c-myc (hybridoma supernatant diluted 1:10, clone 9E10) or with biotinylated lectin Canavalia ensiformis agglutinin (ConA) (Vector Laboratories, France) in 1 μg/ml in TTBS (0.05% Tween-20/TBS pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were next incubated first with a biotinylated goat anti-rabbit IgG or horse anti-mouse IgG (BA-1000 and BA-2000 respectively, Vector Laboratories, USA), then with an avidin-biotin-complex conjugated to horseradish peroxidase (PK-6100 ...
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bioRxiv - Cancer Biology 2022Quote: ... and HRP-polymer-conjugated secondary antibody (anti-Rabbit, Goat and Rat from Vector Labs; anti-Mouse from Biocare. The slides were developed with Impact DAB (Vector Labs) and counterstained with hematoxylin ...
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bioRxiv - Immunology 2021Quote: ... the secondary biotinylated goat anti-mouse Ab was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Color was developed by incubating the slides with freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Neuroscience 2020Quote: ... and the appropriate biotinylated goat anti rabbit or goat anti mouse or secondary antibody (Vector Laboratories, BA-1000, BA-9200, 1:500). The signal was developed using a standard Vectastain ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBST before being incubated for two hours in 1:250 dilution of horse-anti-mouse (Vector Laboratories, UK), or ...
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bioRxiv - Pathology 2024Quote: ... The sections were washed three times with PBS before being placed in blocking solution with a fluorescein horse anti-mouse IgG (H+L) antibody and a Texas red goat anti-rabbit IgG (H+L) antibody (1:200; Vector Laboratories, Inc.). They were left there for two hours at room temperature to find VEGF and CD31 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed with PBST three times and then incubated with biotinylated goat anti-mouse IgG antibody (Vector Laboratories, cat# BA-9200) for 2 hours RT ...
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bioRxiv - Bioengineering 2020Quote: ... Some sections were also immunostained with the antibody to neuron-specific nuclear protein NeuN (MAB377, 1:2K, Chemicon, Temecula, CA) and visualized using chromogen Vector Red (Vector Laboratories, Burlingame, CA). The stained sections were microphotographed using a 3-megapixel digital microscope camera (Spot RT ...
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bioRxiv - Zoology 2020Quote: ... followed by 10-min incubation with streptavidin-HRP (streptavidin conjugated to horseradish peroxidase in PBS containing stabilizing protein and anti-microbial agents, Vector Laboratories Inc., USA). Finally ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed 3 times with cold PBS and resuspended in Vectashield mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Cells were imaged at 100X magnification using a Leica DM IRBE microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS for 3 times and mounted with VECTASHIELD antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, H-1200; Vector Laboratories). Images were taken by SP8 confocal microscopy (Leica Microsystems) ...
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bioRxiv - Genetics 2021Quote: ... Petals were removed from the buds and transferred to a microscope slide with 10ul of Vectashield Mounting Media containing 4’,6-Diamidino-2-Phenylindole (DAPI) (1.5ug/mL) (Vector Laboratories, Canada). A 22×22 mm coverslip was added and mild pressure was applied to the coverslip ...
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bioRxiv - Microbiology 2020Quote: ... The slides were allowed to air dry before placing a drop of Vectashield mounting medium containing DAPI (4’,6-Diamidino-2-phenylindole dihydrochloride) (Vector Laboratories) on them ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides were finally mounted with Vectashield Mounting Media with 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat# H-1200). Stainings were digitalized employing the Olympus BX61VS microscope and scanner software VS-ASW-S6 (Olympus ...
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bioRxiv - Genomics 2021Quote: ... Flies were dissected 10 to 40 days pi and organs viewed by fluorescence microscopy and imaged live or fixed in 2% paraformaldehyde and stained with DAPI in VECTASHIELD mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... unexpanded sections were washed in PBS-T and mounted under Vectashield fluorescence media with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... and then treated with secondary antibodies for 1h before being washed and mounted onto slide glasses with VECTASHIELD Antifade Mounting Medium containing 4’,6-diamidino-2-phenylindole (#H-1200, Vector Laboratory) and sealed using nail polish.
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bioRxiv - Biochemistry 2022Quote: ... 0.02% Triton X-100 and mounted onto slides with Vectashield anti-fade mounting media containing 4’,6-diamindino-2-pheylindole (DAPI, Vector Laboratories). Slides were imaged on a Zeiss Cell Observer widefield fluorescence microscope and analysed using ImageJ.
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bioRxiv - Molecular Biology 2022Quote: ... slides were dried and counterstained with 1.5μg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted in Vectashield antifade mounting medium with DAPI (Vector Laboratories, Piedmont, Italy) before being sealed with a coverslip and nail varnish.
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bioRxiv - Neuroscience 2022Quote: ... incubated in appropriate secondary antibody solutions for 2 hours (ChAT (1:200): rabbit anti-goat IgG (biotinylated; Vector Laboratories, BA-5000); PV (1:200) ...
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bioRxiv - Immunology 2022Quote: Coverslips were mounted on slides with VECTASHIELD Antifade Mounting Medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories; H-1200). Images were obtained using a wide-field DeltaVision Elite microscope with a 100× objective using a PCO Edge sCMOS camera ...
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bioRxiv - Genetics 2022Quote: ... Stained gonads were resuspended in 1 drop of Vectashield containing 4’,6-Diamidino-2-Phenylindole Dihydrochloride (DAPI) (Vector Laboratories H-1200), applied to a large pre-made agarose pad on a glass slide ...
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bioRxiv - Neuroscience 2021Quote: ... We then rinsed the sections in PBS and incubated them for 2 h with biotinylated anti‐rabbit IgG secondary antibody (BA‐1000, Vector Laboratories) diluted 1:600 in 4% BSA in 0.4% PBS‐TX ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... After the transfer the blots were blocked for 2 h in 1 x Animal-Free Blocker (SP-5030 Vector Labs, USA). The blots were incubated over night at 4°C under gentle agitation in the primary antibody solutions (1:300 goat anti-UCP1 ...
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bioRxiv - Developmental Biology 2021Quote: ... For IBA1 staining sections were subsequently incubated with a biotinylated F(ab’)2-Goat anti-Rabbit IgG (Vector Labs, CA, USA). All sections were then incubated with horseradish peroxidase (HRP)-conjugated streptavidin (Dako Agilent Pathology Solutions ...
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bioRxiv - Genomics 2022Quote: ... Cells nuclei were counterstained with 1:15,000 DAPI (4′,6-diamidino-2-phenylindole) at room temperature and then mounted onto slides by using the VectaShield (Vector Laboratories) mounting media ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were mounted with the nuclear dye DAPI (4’, 6-diamindino-2-phenylindole dihydrochloride) in the VECTASHIELD HardSet mounting medium (cat# H-1500, Vector Laboratories). Immunostained slices were imaged using an epifluorescent Nikon eclipse ni microscope and NIS Elements v ...
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bioRxiv - Cell Biology 2020Quote: ... Muscle tissues were then placed into a microcentrifuge tube containing PBT with 2 % final concentration of normal goat serum (NGS; Vector Labs) for 30 mins ...
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bioRxiv - Neuroscience 2022Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories).
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bioRxiv - Genomics 2022Quote: ... Cells were then mounted with coverslips and counterstained with Vectashiled mounting medium containing 4’-6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). RNase A treatments (0.8 mg/mL ...