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Citations for Vector Labs :
1451 - 1483 of 1483 citations for PD 1 Human C93S HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Physiology 2023Quote: ... Muscle sections were incubated in 1:1000 Wheat germ agglutinin (WGA– fluorescein 5 μg/mL; Cat# FL-1021-5, Vector Laboratories Ltd., Peterborough, UK) for 10 min followed by three washes of PBS-T for 5 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were probed with fluorochrome-conjugated secondary antibodies for 1 hour at room temperature and mounted using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). Images were taken with the LSM 880 confocal microscope (Zeiss).
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bioRxiv - Neuroscience 2023Quote: ... slices were rinsed several times using 100 mM PBS and subsequently incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by incubation with biotinylated horse anti-mouse IgG secondary antibody (1:200, PK-6102, Vectastain Elite ABC HRP kit, Vector Laboratories, USA, RRID: AB_2336821) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase activity was reduced using endogenous peroxidase and alkaline phosphatase blocking solution (Blockxol, cat# SP-6000) followed by blocking for 1 hr at room temperature using animal-free blocker (Vector Laboratories, cat# SP-5035). Then the sections were stained with primary antibody diluted in SignalStain Antibody Diluent (Cell Signaling Technology ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... The following day, the sections were incubated with secondary antibodies (1 h, room temperature) and mounted with Vectashield® Mounting Medium (Vector Laboratories, #H-1000). Image acquisition was performed using a Leica SP8 confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min, a first antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, Rabbit α-Phal-L 1:8000 (Vector Laboratories, AS-2300, RRID: AB_2313686), Mouse α-PV 1:4000 (Swant ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed with PBS and incubated for 1 h at room temperature with a protein-blocking solution (VECTASTAIN ABC kit, Vector Laboratories, Burlingame, CA, USA). Excess blocking solution was drained ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were then administered a lethal dose of sedative (sodium pentobarbital) followed by an intravenous injection of DyLight 594-conjugated tomato lectin (100 µl; Vector Laboratories, #DL-1177-1). Mice that received stereotactic surgery were only given injections of tomato lectin (no prior anesthesia/administration of dextran) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were treated with AB solution for 30 min at R/T (Avidin-Biotinylated Horseradish Peroxidase H from Vectastain-ABC KIT of Vector Laboratories 1:200 in PBT).
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bioRxiv - Developmental Biology 2021Quote: Immunohistochemistry was performed as previously described (Frei et al., 2011) using anti-HAND2 antibody (A-12, Sc-398167, 1:100) and secondary universal antibody (Vectastain, Vector laboratories Inc., Burlingame, CA, USA), according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... MA) was used as a marker of hypoxia and lectin (1 mg/kg; DyLight 594 labeled lycopersicon Esculentum tomato, Vector Laboratories, DL-1177, Burlingame, CA) as a marker of ischaemia [17] ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Microbiology 2021Quote: ... the filter pieces were embedded in a 4:1 mix of Citifluor (Citifluor Ltd, London, United Kingdom) and Vectashield (Vector Laboratories, Inc., Burlingame, United States), and stored overnight at −20°C for later microscopy evaluation.
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections (40 µm) through the mPFC were sectioned using a sliding microtome and stained with WFA (1:500, Vector Laboratories, FL-1351, RRID AB_2336875) and rabbit anti-PV antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were incubated with primary antibody overnight at room temperature and detected using the appropriate biotinylated secondary antibody (1:200, Vector Labs, BA-1100, BA-2000) and alkaline phosphatase-Vector Red according to manufacturer instructions (Vector Labs) ...
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bioRxiv - Immunology 2023Quote: Single chain HIV-1 gp140 SOSIP variant strain BG505 [52] was purified over agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5). Column was washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with PBS (3 x 10 min) and incubated with biotinylated goat anti-rabbit secondary antibody (1:600, Vector Laboratories, Cat# BA-1000, RRID:AB_2313606), diluted in 1% NGS in PBS with Triton X-100 ...
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bioRxiv - Cell Biology 2019Quote: ... Immunohistochemistry for F4/80+ was performed using a rat antimouse F4/80+ antibody (1:100 dilution, AbD Serotec®, Raleigh, NC, USA) subsequently incubated with the appropriate secondary biotinylated antibody (Vector Laboratories Inc., Burlingame, CA, USA) and visualized with Immpact AEC peroxidase (Vector Laboratories Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Molecular Biology 2022Quote: ... The medium was then replaced with imaging 2i medium (FluoroBrite DMEM [Thermo Fisher Scientific, A1896701] containing VectaCell Trolox Antifade Reagent for Live Cell Imaging (Vector Laboratories, CB-1000; 1:1,000). After image acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 3 times in PBS and then incubated 1 hour at RT with either ImmPRESS® HRP Horse Anti-Mouse IgG Polymer (Vector Laboratories Cat# MP-7402, RRID:AB_2336528) or ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer (Vector Laboratories Cat# MP-7401 ...
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bioRxiv - Neuroscience 2024Quote: ... as previously described.18 Pan-vascular labeling was achieved through staining with DyLight-594 labeled Lycopersicon Esculentum (Tomato) Lectin (Vector labs, cat. no.: DL-1177-1), which was added to both primary and secondary incubations at 1:100 concentration ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS for 1 h at room temperature and mounted in Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories H-1002-10 and H-1000-10). Images used for analysis were captured using a Nikon E800 fluorescent microscope with a 10x objective ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed twice for 20 min in 2XSSC at 42°C and subsequently incubated with fluorescein anti-biotin antibody (Vector Laboratories, SP-3040; Dilution 1:100) for at least 45min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed and incubated for 2h at room temperature in biotinylated horse anti-mouse antibody (1:500, Vector Laboratories Inc., BA-2001, Burlington, ON, Canada). Then ...
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bioRxiv - Systems Biology 2021Quote: ... #415190-9041-000) were treated with UV light for 1 h and coated with APES (3-aminopropyltriethoxysilane) using Vectabond reagent (Vector Laboratories, Burlingame, CA, USA, cat. #SP-1800-7) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed with 0.1 M PB (4 x 5 min) and incubated in biotinylated goat anti-mouse IgG antibody (Vector Laboratories: 1/200 in 2% NGS in 0.1M PB) for 30 min ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: Fluorescein-conjugated Sambucus nigra lectin (SNA) that binds sialic acid and Erythrina cristagalli lectin (ECL) that binds galactose were acquired from Vector Laboratories (SA-5001-1 and FL-1141-5, respectively).