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Citations for Vector Labs :
1251 - 1300 of 1483 citations for PD 1 Human C93S HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Sections and myofibers that were stained with anti-Pax7 antibody (DSHB) also included a block with 1:50 Mouse on Mouse Ig Blocking Reagent (MKB-2213, Vector Labs).
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bioRxiv - Neuroscience 2023Quote: ... Images were analyzed and edited with ImageJ 2.0.0. (NIH). HRP-labelled sections were revealed (approx. 1 min) using the Diaminobenzidine (DAB) Substrate Kit (Vector Laboratories, SK-4100). The enzymatic reaction was stopped with ddH2O and samples were counterstained with Harry’s hematoxylin (15 s ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a biotin-conjugated anti-rabbit or anti-mouse antibody (1:250, Vectastain Elite ABC kit, Vector laboratories). Sections were counterstained with Nissl (0.09% thionin ...
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bioRxiv - Neuroscience 2023Quote: ... BA-2000)/ goat anti-guinea pig (BA-7000)/ goat anti-rabbit (BA-1000) or horse anti-goat (BA-9500) biotinylated secondary antibodies overnight (1:500, Vector Laboratories) followed by extensive washes in TBS (3×10 min ...
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bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were washed with PBS (10 min, three times) and incubated with biotin-labeled goat anti-rabbit secondary antibody (1:500; Vector Labs) at room temperature for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with Alexa Flour-conjugated secondary antibody for 1 hr at room temperature and mounted in Vectashield mounting medium containing DAPI (Vector Laboratories). Nikon A1 Confocal microscope (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... Chambers were first blocked with 1% Pluronic F-127 for 30 min and then with a mixture of 1 mg/mL β-casein and 0.1 mg/mL avidin DN (Vector Laboratories) for at least 30 min ...
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bioRxiv - Immunology 2024Quote: ... was diluted 1:500 and applied for 30 min at RT followed by biotinylated secondary rabbit anti-goat antibody 1:400 (BA-5000; Vector Laboratories) for 10 minutes ...
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bioRxiv - Genomics 2024Quote: Permeabilization with 0.1% Tween-20 in PBS was performed prior to a 1 hr blocking with a blocking solution containing 10% goat serum (Vector Laboratories) and 3% BSA (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated with 594 (or 488) labeled secondary antibodies (1:200) and DAPI-containing mounting solution VECTASHIELD (Vector Laboratories). The slices were visualized by using a Nikon inverted microscope Eclipse Ti-U equipped with a digital camera ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary AlexaFluor conjugated antibodies (ThermoScientific, 1:400) were used to detect isotype-specific primary antibodies and mounted with Vectashield reagent (Vector Laboratories).
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bioRxiv - Neuroscience 2024Quote: Sections were then incubated in secondary antibody (biotinylated anti-goat IgG made in horse, 1:500, Vector Laboratories, see Table 2) for 1 hr in Tris B ...
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bioRxiv - Neuroscience 2024Quote: ... We then washed the sections three times with PBS (10 min each) and incubated them for 1 hour in avidin-biotin-complex (Vectastain ABC Elite Kit; Vector Laboratories). We washed sections four times in PBS (5 min each ...
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bioRxiv - Neuroscience 2024Quote: ... a gift from Dr. Philippe Ciofi) (Griffond, Deray et al. 1993) followed by biotinylated goat anti-rabbit IgG (1:200, Vector Laboratories). Brain sections were further incubated in avidin-biotin peroxidase solution before reacting with Nickel DAB developing solution ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins were separated by centrifugation at 104,000 g for 1 h at 4 °C and loaded onto a column prefilled with either 2 mL of agarose-bound WFA (Vector Laboratories) for S ...
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bioRxiv - Cell Biology 2020Quote: ... was carried out according to manufacturer’s protocol after fixation of organoids with 0.1% paraformaldehyde in PBS for 1 hour at room temperature followed by blocking with Avidin/Biotin (Vector Labs SP-2001). Biotin-Maackia Amurensis lectin was labeled with streptavidin-PE conjugate (Thermo Fisher SA10041 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Triton-X-100) four times for 20 min each and kept in Vectashield antifade mounting medium with DAPI (Vector laboratories; H1200), at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... On the following day sections were incubated in biotinylated goat anti-rabbit secondary antibody (1:500, #BA-1000, Vector laboratories, RRID: AB_2313606) and avidin-biotin complex (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with 3% NDS-PB-TX and first incubated in biotinylated secondary anti-mouse antibody (1:200, Vector Labs, CAMK-IIα amplification) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was rinsed in TBS-X and reacted with the respective biotinylated secondary antibody (1:200, Vector Laboratories Ltd., Peterborough, UK) for 60 min at room temperature in 1% BSA in TBS-X ...
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... Rinsing with PBS was followed by incubation in a solution containing biotin-conjugated horse anti-goat IgG (1:200 (Vector Laboratories, USA) in 3 % horse serum + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories, PK-6100) overnight at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed 3×5 min in PBS and incubated with 1:1000 anti-rabbit biotinylated secondary antibody (BA-1000, Vector Laboratories, USA) in 1% NGS in PBST for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800; Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was washed 3 times for 5 minutes in 0.1 M PB and placed in goat anti-rabbit OR anti-mouse biotinylated secondary antibodies respectively (1:500, BP-9200/9100; Vector Labs, CA) for 6 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit, Vector Laboratories, PK6100). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Biochemistry 2021Quote: ... or mouse anti-c-myc (hybridoma supernatant diluted 1:10, clone 9E10) or with biotinylated lectin Canavalia ensiformis agglutinin (ConA) (Vector Laboratories, France) in 1 μg/ml in TTBS (0.05% Tween-20/TBS pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C in blocking solution and biotinylated anti-rabbit IgG secondary antibody (1:3000, Vector Laboratories BA-1000) at RT for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were stained using Wisteria floribunda agglutinin coupled to fluorescein (WFA; 1:100; cat# FL-1351, RRID: AB_2336875, Vector Laboratories, CA, USA) for 12h (at 4°C) ...
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxide blocking was performed in 1% water for 15 minutes before blocking in 2.5% normal horse serum (Vector Laboratories -MP-7401) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 1h incubation at room temperature with a 1:250 dilution of an avidin-biotin complex solution (ABC Vectastain kit, Vector Laboratories®). Revelation was performed using the DAB Peroxidase Substrate Kit (DAB SK4100 kit ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue sections were rinsed again in TS and then incubated in biotinylated WFA (Vector Laboratories, Cat# B-1355, RRID: AB_2336874, 1:50) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... RGCs were purified using two-step immune-panning on two Lectin coated negative panning plates (Bandeiraea simplicifolia, BSL-1; Vector Laboratories Ltd.) and a Thy1.2 antibody coated positive plate (Serotec MCA02R ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue sections were rinsed again in TS and then incubated in biotinylated WFA (Vector Laboratories, Cat# B-1355, RRID: AB_2336874, 1:50) for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... the secondary biotinylated goat anti-mouse Ab was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Color was developed by incubating the slides with freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Neuroscience 2020Quote: ... they were subjected to incubation with the secondary antibody (biotinylated goat anti-rabbit, 1:500, Cat no. BA9400, Vector Labs, United States) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed for 3 x 10 min (PBS-TX) and incubated with the secondary antibody goat anti-rabbit (1:200) (conjugated to DyLight 594; Vector Laboratories, CA) for two hours ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed again (3 times in PBS/Tween, 5 minutes each) and incubated in biotinylated goat anti-rabbit (1: 200, Vector Laboratories, USA) or biotinylated horse anti-mouse (1 ...
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bioRxiv - Neuroscience 2021Quote: The sections were blocked for 1 h at room temperature in 0.5% Triton-X100 in PBS (PBST) and 10% normal goat serum (Vector Laboratories, S-1000) solution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed and incubated overnight with the secondary biotinylated antibody (BA-1000, goat anti-rabbit IgG, Vector Laboratories, 1:1000). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were permeabilized with 0.5% Triton X-100 for 30 min at room temperature and blocked in 5% goat serum (Vector Laboratories, 1:200), goat anti-rat 488 and goat anti-mouse 647 (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti-Ntla immunostaining was performed after in situ hybridization using rabbit anti-Ntla (Schulte-Merker et al., 1992, 1:2000) and Horse anti-rabbit IgG-HRP conjugated (Vector Laboratories, Inc.)
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bioRxiv - Neuroscience 2019Quote: ... pH 6), followed by an incubation with rabbit anti-Iba1 (WAKO, Japan; 1:2500) and subsequently a biotinylated secondary antibody (Vector laboratories, USA). Visualization ensued after using the ABC complex solution (Vector laboratories ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Non-specific binding sites were blocked for 1 hour (Col2: MoM Kit, Vector Labs, BMK-2202; Sox9: 10% v/v goat serum). Primary antibodies were incubated overnight at 4°C and included Col2 (1/100 ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories,UK) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... and the appropriate biotinylated goat anti rabbit or goat anti mouse or secondary antibody (Vector Laboratories, BA-1000, BA-9200, 1:500). The signal was developed using a standard Vectastain ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA) followed by Vectastain Elite ABC HRP (Vector labs ...