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Citations for Vector Labs :
1151 - 1200 of 1482 citations for Enolase 1 Alpha enolase Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in biotinylated secondary antibody diluted 1:500 in block (biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories). Sections were washed in TBS and then incubated for 1 hour at RT in Vectastain kit components A and B ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the samples were washed with 1× TBS followed by incubation for 1 h at room temperature with a biotinylated secondary antibody (PK-6102, horse serum, mouse IgG or PK-6101, goat serum rabbit IgG; 1:3000 dilution; Vector Laboratories) in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The slide was then rinsed by ice-cold ethanol/acetic acid (3:1) and mounted in Vectashield with DAPI (Vector Laboratories) to observe the DNA.
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bioRxiv - Neuroscience 2019Quote: ... After staining with Neurotrace 435/455 (1:100 in PBS) and mounting on gelatin-coated slides in Vectashield (catalogue no. H-1400; Vector Laboratories), sections were imaged with a Fluoview FV-1000 confocal imaging system (Olympus ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed (3 × 5 min) in 0.1M PBS at RT, followed by incubation with secondary antibody (biotinylated goat anti-rabbit, (1:400; Vector Laboratories, BA1000) in blocking solution at RT for 1h ...
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bioRxiv - Pathology 2019Quote: ... slides were washed in PBS and the biotinylated rabbit antibody (1:200 dilution, VECTASTAIN® Elite® ABC Kit, Vector Laboratories) was applied in blocking solution for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval was performed in the Biocare Medical LLC decloaking chamber (NC0436641) for 1 hour with citrate-based antigen retrieval buffer (H-3300, Vector Laboratories) and washed with PBS ...
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bioRxiv - Bioengineering 2020Quote: ... sections were washed in PBS and then incubated with a biotinylated goat anti-rabbit secondary antibody (1:200, Vector Laboratories, BA1000) for one hour at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Three drops of normal goat serum in 10ml PBS was used as blocking buffer for 1 hr at room temperature before primary antibodies incubation (Vectastatin ABC Kit; Vector Laboratories). Slides were then incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... To isolate OPC the single cell suspension was sequentially panned on two panning plates coated with anti-BSL 1 Griffonia simplificonia lectin (#L-1100, Vector Labs) and then incubated on a plate coated with anti-CD140a (#135902 ...
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bioRxiv - Neuroscience 2021Quote: Sections used for morphological analyses in Figure 1C were further incubated in a solution of conjugated avidin-biotin horseradish peroxidase (ABC; 1:300; Vector Labs) in TBS (pH = 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS + 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were mouse anti-abd-A (gift of Ian Duncan) ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200, Vector Laboratories, Burlingame, CA, USA), and then incubated for 1h in an avidin-biotin peroxidase complex (Vectastain ABC Elite PK6100 ...
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bioRxiv - Neuroscience 2020Quote: ... Corresponding secondary antibodies (1:500 goat anti-rabbit/goat anti-mouse biotinylated secondary antibody (Vector Laboratories, BA-1000 and BA-9200) were added ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were then washed with PBS and mounted using DAPI (1 μg/ml) or VECTASHIELD with DAPI Mounting Medium (Vector Laboratories). Images were captured using a Zeiss 780/880 confocal microscope.
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed and transferred to donkey anti-rabbit secondary antibody (1:500; Jackson Immuno) for 2 hr before detection with ABC (Vectastain ABC Kit, Vector Laboratories) and visualization with DAB substrate (Vector Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were again rinsed in PB and then incubated overnight in biotinylated donkey anti-sheep IgG in PB (1:200, Vector Labs), followed by overnight incubation in streptavidin-conjugated Alexa 488 in PB (1:800 ...
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bioRxiv - Microbiology 2020Quote: ... followed by a 1h incubation of the following primary antibodies diluted in blocking buffer: biotinylated-dolichos biflorus agglutinin (DBA) [1:500] (Vector Laboratories), rabbit-anti SRS9 [1:1000] and mouse-anti SAG1 [1:1000](kindly provided by John Boothroyd ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed 3 times in PBS and the secondary antibody (biotinylated horse anti-rabbit; 1:250, Vector Laboratories, Burlingame, CA) was added and slides were incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... were applied for 1 h at 37°C prior to mounting onto slides using mounting media containing DAPI (Vectashield, Vector Labs). Images were obtained on a Leica SP5 confocal microscope or a Nexcelom Celigo as described in individual figure legends.
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bioRxiv - Neuroscience 2020Quote: ... 30 min in biotinylated goat anti-mouse or anti-rabbit IgG (1:200, PA-920 or BA-1000; Vector Laboratories, USA); 30 min avidin-biotin complex solution (PK-7100 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The homogenate was blocked using 3% bovine serum albumin (BSA) in 0.2% Triton X-100 before staining with 1:250 rhodamine-conjugated Dolichos biflorus lectin (Vector Laboratories Inc.) to visualize the cyst wall ...
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bioRxiv - Immunology 2021Quote: ... Germany) for 60minutes at a 1:1500 dilution and followed by a biotinylated anti-rat (Cat. BA-5000, Vector Laboratories, Inc.) for 15 minutes at a 1:2000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 1-hour in an avidin biotin complex solution (per kit instructions, ABC Peroxidase, PK-6100, Vector Laboratories) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.1 M PB, for 10 min) to reduce background auto-fluorescence (Clancy & Cauller, 1998) before being mounted in VectaShield (Vector Laboratories) and later imaged using an epifluorescence system (Leica DM 5000 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in appropriate secondary antibody solutions for 2 hours (ChAT (1:200): rabbit anti-goat IgG (biotinylated; Vector Laboratories, BA-5000); PV (1:200) ...
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bioRxiv - Neuroscience 2022Quote: ... staining was enhanced after primary antibody incubation with biotinylated secondary antibodies (biotinylated horse anti-goat IgG, Vector Laboratories: BA-9500, RRID: AB_2336123; 1:300; biotinylated goat anti-rabbit – bGAR, Vector Laboratories: BA-1000 ...
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bioRxiv - Neuroscience 2022Quote: ... all brain sections were transferred into the secondary antibody solution (1:200 in PBS; biotinylated anti-rabbit made in goat, BA-1000 Vector Laboratories) for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or CD4 (ab183685, 1:400 dilution overnight at 4 degrees).Tissues were then counterstained with biotinylated anti-rabbit secondary (Vector Labs, BA-1000 ...
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bioRxiv - Genetics 2022Quote: ... Stained gonads were resuspended in 1 drop of Vectashield containing 4’,6-Diamidino-2-Phenylindole Dihydrochloride (DAPI) (Vector Laboratories H-1200), applied to a large pre-made agarose pad on a glass slide ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... After the transfer the blots were blocked for 2 h in 1 x Animal-Free Blocker (SP-5030 Vector Labs, USA). The blots were incubated over night at 4°C under gentle agitation in the primary antibody solutions (1:300 goat anti-UCP1 ...
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bioRxiv - Immunology 2020Quote: RBC ghosts were suspended in equal volumes of calcium binding buffer containing 2% Triton X-100, pre-cleared with magnetic streptavidin beads (88816, Pierce) and incubated with biotinylated GNA lectin (1 mg/ml; B1245, Vector Laboratories), biotinylated MAL-II lectin (1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoperoxidase detection was then performed by enzymatic reaction with an avidin-peroxidase complex for 1 h (Vectastain ABC HRP Kit Peroxidase Standard, Vector Lab.). After washing in PBS ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories). Slides were developed with Bio-Red (Biopath Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories). Slides were developed with Bio-Red (Biopath ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were thoroughly washed and incubated for 1 h with the appropriate secondary antibody: biotinylated anti-rabbit (BA-1000, Vector Laboratories) or biotinylated anti-mouse (BA-2000 ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then washed thrice in phosphate buffered saline prior to 30 min incubation with biotin-conjugated Goat Anti-Mouse IgG (H+L) (1:500, BP-9200, Vector Laboratories). Endogenous peroxidase was blocked and detection was performed using VECTASTAIN® ABC-HRP Kit with signal development carried out using 3,3-diaminobenzidine ...
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bioRxiv - Physiology 2022Quote: ... After reducing non-specific binding with 30 min of Animal-Free Blocker incubation followed by 1 h incubation with Mouse on Mouse Blocking Reagent (Vector Laboratories), sections were incubated with primary antibodies (Proinsulin ...
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bioRxiv - Physiology 2019Quote: ... at a dilution of 1:25 for 20 min using agitation followed by 3 washes with PBT and 1 wash in PBS before mounting in Vectashield with DAPI (#H-1200, Vector Laboratories). Analysis of the tissue and image acquisition was carried out using a Zeiss LSM 710 confocal laserscanning microscope and processed using Fiji and Adobe Photoshop.
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bioRxiv - Molecular Biology 2020Quote: ... cells were stained with 1 µg/ml DAPI and mounted with VECTASHIELD® Antifade Mounting Medium (#H-1000-10, Vector Laboratories). Images were taken using a Perkin Elmer spinning disc confocal equipped with a Nikon-TiE inverted microscope using 60X objective oil lens with an Orca ER CCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Synthetic Biology 2021Quote: ... slides were again washed 5 times for 10 min with 1 × PBS at room temperature and mounted in Vectashield (Vector Laboratories) with 1 µg/ml DAPI ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV cross-reactive N protein 1C7C7 MAb (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was used at 5 μg/mL final concentration for 1 hour at 24°C following by goat anti-rabbit IgG biotin conjugate used as secondary antibody at 1-200 final dilution (BA-1000, Vector Laboratories). DAB Map including 3,3-Diaminobenzidine chromogen was applied to the slides for histological staining.
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with biotinylated anti-goat antibody IgG produced in rabbit (1: 1000; Alexa 594, Vector Laboratories, Burlingame, CA, USA) for 1 hour ...