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Citations for Vector Labs :
1101 - 1150 of 1482 citations for Enolase 1 Alpha enolase Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the slides were incubated with Vectastain ABC-peroxidase (ABC-PO, from Vector Laboratories; PK-4000; diluted 1:100 in PBS) for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then incubated in biotinylated α mouse (6E10) or rabbit (AT8) secondary antibody (1:200; Vector Labs, Burlingame, CA) for 2 hrs at room temperature followed by Vectastain Elite ABC reagent (Vector Labs ...
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bioRxiv - Immunology 2021Quote: ... washed and stained overnight in a 500μL PBS solution containing 1:150 dilution of FITC-conjugated Dolichos biflorus agglutinin (Vector Laboratories) with slow rotation at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation of the sections in biotinylated anti-rabbit/mouse secondary antibody (1:1,000 dilution, Vector Laboratories, Burlingame, CA) and then in avidin-biotin-peroxidase complex (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... and Ki67 immunostaining signals were further amplified by tyramide-FITC or tyramide-Cy3 (1:2,000, Perkin-Elmer, Wellesley, MA) after avidin-biotin complex incubation (Elite ABC kit, Vector Laboratories). Immunostained sections were counterstained with DAPI (1 μg /ml ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was washed by PBS-TX (0.3% Triton X-100) and then incubated with 1:1000 dilution of biotinylated anti-mouse antibody (Vector Labs) with agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary detection was performed with: Streptavidin DyLight 488 nm (1:200 dilution, Vector Laboratories, Burlingame, CA, catalog no. SA-5488), Goat anti–Rat Alexa 546 nm (catalog no ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# 111-035-144] and Peroxidase-conjugated horse anti-mouse IgG (H+L) antibody [(1:5,000) (Vector laboratories, PI-2000)].
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bioRxiv - Neuroscience 2022Quote: ... DAPI was applied at 1:10,000 dilution for 45-minutes and sections mounted with VectaShield HardSet (H-1400, Vector Laboratories). Antibodies included Activated Beta Catenin (05-665 ...
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bioRxiv - Physiology 2022Quote: ... EDL and TA sections were stained with 1:1000 fluorescein wheat germ agglutinin (WGA; 5 μg/mL; Vector Laboratories Ltd.) for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and analyzed by lectin blotting using biotinylated concanavalin A (Con A) lectin (1:10,000, Cosmo Bio) and VECTASTAIN ABC-HRP kit (PK-4000, Vector Laboratories) or by Western blotting using primary antibodies and secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... washed and incubated in horse anti-mouse biotinylated secondary (1:200 in 2% BSA and 0.1% Triton {TBS]; Vector Laboratories) for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS] ...
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bioRxiv - Neuroscience 2024Quote: ... brains were mounted on slides between two number 1 glass coverslip pieces before covering with VectaShield mounting medium (Vector Laboratories) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated at RT for 1h with biotinylated anti-rabbit (1:500; BA-1000; Vector Laboratories, Inc; Burlingame, CA), biotinylated anti-rat (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 10 min three times (0.2 µg mL−1 DAPI in the second wash) and mounted with Vectashield PLUS Antifade (H-2000, Vector Laboratories) or ProLong Gold (Life Technologies).
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bioRxiv - Neuroscience 2024Quote: ... After an hour of cool-down slides were washed with PBS and incubated with blocking buffer (1% BSA, 5% FBS, 0.01 % Triton-X 100 in 1x PBS) followed by Avidin/Biotin blocking (Vector laboratories). Slides were incubated overnight at 4°C with primary antibodies (see Supplementary Table 4 ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were blocked for 1 h at room temperature in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) diluted in 0.01 M PBS/0.1% Triton-X permeabilization buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in PBST (3 × 5 min.) and then incubated in 1% ABC solution (Vectastain, Vector Laboratories, Burlingame, CA) and washed again in PBST (3 × 5 min.) ...
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bioRxiv - Neuroscience 2022Quote: ... and counterstained with 1 μg/mL DAPI (Thermo) for 2 minutes to label nuclei and mounted with VectaShield (Vector Laboratories). Images were taken using a confocal microscope (Zeiss LSM 800) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then incubated for 1 h at room temperature with biotin-conjugated goat anti-mouse secondary antibody (Cat#BA-9200-1.5, Vector Laboratories) at 1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in a secondary antibody solution containing 1:500 biotinylated rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated with the biotinylated secondary goat anti-rabbit antibody (1:200 BA-1000, Vector Laboratories) 2 hours at RT ...
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bioRxiv - Plant Biology 2023Quote: ... slides were washed 3 x 5 min in 1 x PBS and mounted in Vectashield mounting medium with 1.5 μg/mL DAPI (Vector Laboratories). Antibodies and dilutions used in this study are reported in Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was performed at 60°C in citrate-based antigen unmasking solution diluted 1:100 (Vector Labs, Burlingame, CA) for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and stained with 2 µg mL-1 4′,6-diamidino-2-phenylindole (DAPI) in Vectashield (Vector Laboratories, Newark, CA, USA). The cells were enumerated using a Zeiss Axio Imager M2 epifluorescence microscope (Carl Zeiss AG ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed three times in 0.2% Triton X-100 in 1xPBS for 10 min and incubated with biotinylated goat anti-rabbit (1:1000, Vector Laboratories) for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were placed in 1× PBS with 0.1% Tween-20 with Vectashield and DAPI mounting medium (Vector Lab; H-1200) (1:30 dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in PBS-T and incubated in blocking solution containing DyLight 488 Streptavidin (1:250, Vector Laboratories) for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... followed by 1 h incubation with ImmPRESS VR anti- rabbit IgG HRP polymer detection kit (MP-6401-15; Vector Laboratories). Chromogenic detection in situ was performed using ImmPACT DAB EqV peroxidase substrate solution (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... the tissue was incubated in ABC solution (1 drop of solutions A and B/10 mL, Vector Laboratories, PK-6100) for 1 h at room temperature followed by development in 0.05% 3,3′-diaminobenzidine (DAB) ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were washed 3 times for 10 minutes each in 1 mL of 1X PBST (0.2% Tween 20 in 1x PBS) and incubated in Vectashield with DAPI (Vector Laboratories) for at least 1 hour before mounting.
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bioRxiv - Neuroscience 2023Quote: ... After incubation for 120min at room temperature in a horse anti-rabbit secondary antibody (1/200; BA-1100, Vector laboratories), sections were processed with avidin-biotin-peroxidase complex (1/200 ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the sections were incubated with anti-rabbit secondary antibody (1:200 dilution; 4°C; 17h.; Vector Laboratories, Burlingame, USA) followed by tertiary labelling with avidin biotin complex (1:200 ...
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bioRxiv - Physiology 2023Quote: ... the sections were incubated with biotinylated Sambucus Nigra Lectin (SNA) in 1% BSA/PBX at pH 6.8 (ZF0207; Vector Laboratories) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were rinsed and incubated for 30 minutes with α-rabbit IgG secondary antibody (1:1000; Vector labs, PK-4001) diluted in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... 10min each) followed by incubation in a secondary antibody solution (1:3000, peroxidase labeled anti-mouse, PI-2000, Vector laboratories). This was followed by rinsing in TBST ...
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bioRxiv - Biochemistry 2024Quote: ... Protein levels were determined by incubating the membrane with ClpL-specific antibodies (1:10,000 in TBS-T + 3% (w/v) bovine serum albumin) and an anti-rabbit alkaline phosphatase conjugate (Vector Laboratories) as the secondary antibody (1:20,000) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells treated with extensive washes with PBS with 1% BSA prior to mounting in anti-fade media (Vector Laboratories, Inc.). The samples were then imaged using confocal microscopy with a LSM 710 laser scanning confocal microscope (Carl Zeiss ...
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bioRxiv - Bioengineering 2024Quote: ... with 0.1% Triton-X and then incubated in the same buffer containing lectin-fluorescein (0.5 mg/ml, FL-1171, Vector Laboratories), overnight for spinal cords and two days for brains ...
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bioRxiv - Neuroscience 2024Quote: ... full-thickness colon segments of ∼1cm in length were blocked for 2 hrs in 1.5ml tubes containing 1 drop of Mouse-on-Mouse blocking reagent (MOM; Vector Labs) in 1ml of PBS with 0.5% Triton-X 100 ...
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bioRxiv - Immunology 2024Quote: ... membranes were washed and incubated with an HRP-conjugated anti-rabbit secondary antibody (PI-1000; 1:40,000 dilution; Vector Laboratories). Protein signals were detected on film and quantified using ImageJ software ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol before samples were stained with DAPI (1 µg/ml, 5 min) and mounted in Vectashield (Vector Laboratories). Images were collected with a Leica SP5 II confocal microscope using LAS-AF software (v2.7.3.9723) ...
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bioRxiv - Neuroscience 2024Quote: ... β-amyloid was detected using thiazine red (1 µM, Cat# 2150-33-6, Chemsavers) associated with DAPI (0.5 µM, Cat# 1485, Vector lab) and a mouse monoclonal antibody against VSM α-smooth muscle actin (Cat# ABT1487 ...
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bioRxiv - Microbiology 2020Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the N 1C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:3000 in PBS 1X and then mounted with Vectashield antifade medium (cat. H-1000 - Vector Laboratories, Burlingame, CA, USA) and analysed immediately or stored at +4°C until ready for confocal image analysis.
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 1 hour at room temperature in 3% normal donkey serum (NDS, Vector Laboratories, Inc., Burlingame, CA, USA) and overnight at 4°C in the rabbit polyclonal antibody directed against calretinin (1:300 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Microbiology 2022Quote: ... fixed in 4% paraformaldehyde for 15 minutes then stained with a 1:500 dilution of dolichos bifluorus agglutinin conjugated to FITC in PBS (Vector Labs). FITC-positive cysts were confirmed by fluorescence and morphology under 20x magnification and the total cyst burden per brain was back-calculated.
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bioRxiv - Neuroscience 2021Quote: ... or Alexa 647 donkey anti-mouse (1:200, Thermo A31571) and then mounted with the VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratory).
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200, BA-1000, Vector Laboratories). Color reaction was performed using the Vectastain Elite ABC Kit (PK-6100 ...