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Citations for Vector Labs :
851 - 900 of 955 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Muscle tissues were then placed into a microcentrifuge tube containing PBT with 2 % final concentration of normal goat serum (NGS; Vector Labs) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... two times for 2 min each before reacting with diaminobenzidine in the presence of nickel (DAB Peroxidase Substrate Kit, Vector Laboratories) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed (three times in 1xPBS for 10 min) and blocked for 2 hours with 10% normal goat serum (NGS, Vector Laboratories), 0.2% BSA (Carl Roth ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed in 1x PBS three times for 2 minutes and covered with Vectashield mounting medium without DAPI (Vector Labs). Five imaging fields in the gray matter of the same case were captured using a fluorescent microscope (Keyence BZ-X710) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were washed three times with 1x PBS at RT before either staining with 3uM DAPI (4’,6-diamidino-2-phenylindole) in 1x PBS for 10 min or mounting in Vectashield fluorescent mounting medium with DAPI (Vector Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... The cells were then postfixed in cold methanol and mounted using Vec-taShield containing 4ʹ,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Images were acquired by a DMI6000B microscope and a DFC360 FX monochrome camera (both Leica Mi-crosystems).
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bioRxiv - Pharmacology and Toxicology 2024Quote: Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories).
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bioRxiv - Bioengineering 2022Quote: ... incubation was for 2 hours at RT and secondary antibody (1:2000 in 1% BSA, Biotinylated horse anti-mouse, Vector Labs) incubation was for 1 hour at RT ...
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bioRxiv - Genomics 2022Quote: ... sections were incubated with Alexa dye–conjugated secondary antibodies diluted in blocking serum for 2 hours before being washed again and mounted with Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories). Alexa Fluor® 488 Donkey IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS-T and then incubated for 2 h at RT with biotinylated secondary antibody (1:200, Vector Laboratories). After subsequent washes with PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... and hybridized with 2 µg/ml digoxigenin (DIG)- and dinitrophenol (DNP)-labeled probes in a hybridization buffer containing the Biotin solution (Vector Laboratories) overnight at 60°C in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were dehydrated by incubating for 2 min in increasing concentrations of EtOH (70%, 95%, and 100%) before mounting with Vectashield with DAPI (Vector laboratories). Images of at least 150 nuclei were acquired at 20X using a Zeiss Imager.M2 epifluorescence microscope equipped with a Axiocam 503 mono camera ...
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bioRxiv - Genomics 2024Quote: ... cells were stained with DAPI 2 μg per ml and mounted into slides using Vectashield (Vector Laboratories Cat # H-5700-60). Images were obtained with a Leica TCS-SL confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: Sections were then incubated in secondary antibody (biotinylated anti-goat IgG made in horse, 1:500, Vector Laboratories, see Table 2) for 1 hr in Tris B ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then incubated for 2 hours at room temperature with horseradish-peroxidase-labelled secondary antibody (Goat Anti-Rabbit IgG Antibody; Vector Laboratories) diluted 1:10,000 in 5% dried non-fat milk powder in TBS-T ...
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bioRxiv - Biophysics 2024Quote: ... Pel in the matrix of PA14 wild-type and ΔwspF was stained using GFP fluorescently labelled Wisteria floribunda lectin (WFL, FL-1351-2, Vector Laboratories). WFL is diluted in phosphate-buffered saline solution at 10 µ/ml concentration and loaded into a syringe ...
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bioRxiv - Cell Biology 2024Quote: ... Histopathology for three biotinylated mucin O-linked specific lectins: Griffonia (Bandeiraea) Simplidifolia Lectin I (GSL I, Vector Labs, B-1105-2); Maackia Amurensis Lectin II (MAL II ...
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bioRxiv - Microbiology 2022Quote: ... The treated samples were incubated with primary antibodies at 4°C overnight followed by universal HRP-conjugated secondary antibodies (Vectastain® Elite ABC-HRP kit, Vector Laboratories, Inc., Burlingame, CA, USA). Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Molecular Biology 2021Quote: All slides were washed three times for 5 min at RT and dried before mounting with the DAPI-containing VectaMount Permanent Mounting Medium (Vector Laboratories, Burlingame, CA, USA), and sealed with nail varnish ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 × 5 min in PBS followed by blocking in 20% normal goat serum (NGS; Vector Labs S-1000, Burlingame, CA, USA) in PBS for 1 hr at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 × 5 min in TBS buffer and then incubated for 100 min in a solution of streptavidin Dylight 649 (Vector laboratories, SA5649, 1:100) in TBS buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibody was decanted and slides washed three times with PBS for 5 minutes each followed by VECTASHIELD® Antifade Mounting Medium with DAPI (Vector Labs H-1200), sealing coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed two more times for 5 min in 1x PBS and mounted with Vectashield mounting media (Vector Laboratories, # H1000, Burlingame, CA, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked with 5% BSA and then incubated with biotin-labeled Maackia amurensis lectin II (MAL-II biotin conjugate, Vector Laboratories, Inc., Burlingame, CA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Paraffin-embedded tissues were sectioned at 5 µm and subjected to IHC using Vectastain Elite ABC Peroxidase (HRP) Kit (Vector Laboratories Cat # PK-6102) as previously described [40] ...
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bioRxiv - Immunology 2022Quote: Clarified cell supernatant was adjusted to a final concentration of 100 mM NaCl and then incubated with Galanthus Nivalis Lectin Agarose (Vector Laboratories, AL-1243-5), washed with 10 column volumes of 20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Endogenous peroxidase was blocked for 5 mins before incubation with 2.5% Normal Goat Serum (ImmPRESS Rat kit – Vector Laboratories, San Francisco Bay Area, USA) TRU for 20 mins ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with 1X PBS for 5 minutes each wash and then mounted using a DAPI-staining mounting medium (Vector Laboratories; H-1200-10). Cells were imaged using Leica DMI4000B microscope fitted with Leica TCS SPE laser-scanning confocal system with Solid State lasers (405/488/561/635 ...
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bioRxiv - Microbiology 2021Quote: ... Worms were washed in PBS three times for 5 mins and transferred to new 12 well plates with 1ml of Fluorescein labeled Aleuria Aurantia Lectin (Vector labs cat# FL-1391) and Rhodamine labeled Griffonia Simplicifolia Lectin I (Vector labs cat# RL-1102 ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... Removal of α(2,3)-linked and α(2,6)-linked sialic acids on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 μg/ml; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the slides were washed three times with PBS (5 minutes each at RT) and mounted using Vectashield antifade mounting media with DAPI (Vector Laboratories, #H-1200-10). To secure the coverslips ...
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bioRxiv - Neuroscience 2022Quote: ... antigen retrieval was performed by using either formic acid for 5 minutes at room temperature or citric acid pH=6 (Vector Laboratories; Cat# H-3300) for 15 minutes at 95 °C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS three times for 5 min each and mounted on slides with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10) and coverslipped ...
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bioRxiv - Pathology 2024Quote: ... sections were stained with Flash Phalloidin™ and autofluorescence quenched for 5 min with the Vector® TrueVIEW™ autofluorescence quenching kit (Vector Laboratories), then mounted with Prolong Diamond Antifade Mountant with DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were rinsed in distilled water and PBS for 5 minutes and then blocked in 10% normal goat serum (Vector Laboratories S-1000-20) in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissues were then washed 3 times for 5 minutes each in PT and mounted in Vectashield with DAPI (Vector Laboratories; H-1200-314 10).
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bioRxiv - Developmental Biology 2024Quote: ... rehydrated and subjected to antigen retrieval using a pre-heated (5 minutes at 100% microwave power) 1/1000 dilution of citrate-based antigen unmasking solution (Vector Labs, H-3300-250) for 20 minutes in the microwave at 10% power ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit, Vector Laboratories, PK6100). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Neuroscience 2022Quote: ... then mounted on slides in hard set mounting medium containing 4,6-diamidino-2-phenylindole (DAPI) (Vectashield, H-1500, Vector Laboratories, Burlingame, CA). A confocal system (TCS SP8 ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... A small aliquot of embryo pellet (2 μL) was taken right before freezing and mixed with 10 μL VECTASHIELD® Antifade Mounting Medium with DAPI (Vector laboratories) and immediately frozen in dry ice ...
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bioRxiv - Microbiology 2021Quote: ... incubated with avidin-biotin complex kit (ThermoFischer Scientific, 32020) for 2 hrs and visualized with a 3,3’-diaminobenzidine kit (Vectastain, Vector Labs, SK-4100). Sections were then counterstained with cresyl violet for Nissl labeling.
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in a secondary solution containing 1:2 ImmPRESS® (peroxidase) polymer horse anti-mouse IgG (rat absorbed) (Vector Laboratories) in TBS for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Then a secondary biotinylated goat anti-rabbit antibody was applied in PBST / 2% NGS (1:200; Vectastain, Vector Laboratories, Burlingame, CA, USA) (75 min ...
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bioRxiv - Neuroscience 2023Quote: ... 30-80 MΩ) lowered vertically and filled with a solution of potassium acetate (0.5M) and Neurobiotin (2%, Vector Laboratories, Burlingame, CA, USA). The signal was amplified and low-pass filtered (10kHz ...
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bioRxiv - Microbiology 2024Quote: ... The parasitophorous vacuoles (PV) of the parasites of were then stained with 2 μg/mL fluorescein labelled Vicia Villosa lectin (VVL; Vector Laboratories #ZG0124) in 1% BSA/PBS for 1 hour at room temperature then nuclei stained with 2 μg/mL DAPI (Merk #096M-4014V ...