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Citations for Vector Labs :
701 - 750 of 955 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... mouse pancreatic sections were incubated overnight at 4°C in guinea pig anti-insulin antibody (1:800) and visualized using the diaminobenzidine peroxidase substrate (Vector laboratories, Burlingame, CA) and hematoxylin staining ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% TritonX-100 in PBS) for 30 min at RT followed by another blocking using M.O.M kit (Vector Laboratories BMK-2202). Primary antibodies (mouse anti-MYH3 ...
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bioRxiv - Cell Biology 2020Quote: ... The organoids were centrifuged at 500 x g for 5 min and resuspended in 30 μL Vectashield (Vector Labs; H-1000) using a cut pipet tip and pipetted onto a coverslide ...
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bioRxiv - Genetics 2021Quote: ... slides were washed 3 times in PBS-T for 5 min each and incubated with the biotinylated horse anti-mouse IgG antibody (AK-5002, Vector laboratories) for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then washed 3 times in PBS for 5 min and Cy5 Streptavidin (1:500 in PBS, No: SA-1500, Vector Lab) was applied for 25 min followed by three washes in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... and treated with or without PNGase F was assayed by lectin blotting using Concanavalin A (Con A) (Cat. B-1005-5, Vector Laboratories). A total of 10 μg protein each were electrophoresed in a 12% SDS-PAGE gel and transferred onto PVDF membranes at a constant of 300 mA for 2 h ...
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bioRxiv - Genetics 2021Quote: ... then blocked with 5% (v/v) horse serum (ImmPRESS HRP Anti-Rabbit IgG [Peroxidase] Polymer Detection Kit; Vector labs; MP-7401). Sections were incubated with rabbit anti-TCFL5 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNMT3A and DNMT3B was visualized in cross-section (5 µm) of paraffin-embedded tissues using specific primary antibodies and a Vectastain Elite ABC Kit (Vector Laboratories) or AlexaFluor 488-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: Sections were briefly dried then washed 3x in 0.1% Triton X-100 in phosphate buffered saline (PBST) before they were blocked with 5% neutral goat serum (Abcam or Vector Labs). Sections were then incubated for double-labeling experiments with primary antibody (AB ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were washed 3 times for 5 min with PBS-T and incubated with 60 μg/ml PSA-FITC (FL 1051, Vector Laboratories) in PBS for 30 min in dark at room temperature for acrosome labelling ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed in PBS three times for 5 min each before being placed in a blocking solution containing 3% normal goat serum (Vector Laboratories) for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... Processed spinal cord slides were incubated with DAPI (made up in water) for 5 minutes in a hydration chamber at room temperature and a coverslip was mounted with vectoshield (Vector labs). Process slides were imaged on a Leica SP8 confocal microscope.
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bioRxiv - Cell Biology 2021Quote: ... Tween 20 (TBST) for 5 min each and incubated at room temperature for an hour in secondary antibody-HRP (Vector Laboratories). Antibodies were then visualized by peroxidase substrate kit DAB (Vector Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... three times for 5-min and then blocked for 30-min with 30% normal goat serum (Vector Labs, cat# S-1000) and 1X PBS/Triton 0.3% ...
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bioRxiv - Synthetic Biology 2021Quote: ... slides were again washed 5 times for 10 min with 1 × PBS at room temperature and mounted in Vectashield (Vector Laboratories) with 1 µg/ml DAPI ...
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bioRxiv - Immunology 2020Quote: ... tissues were washed twice for 5 minutes in wash buffer and stained using the ImmPRESS universal (Anti-Mouse/Anti-Rabbit) kit (Vector labs).
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bioRxiv - Cell Biology 2021Quote: ... cells were washed twice for 30 min with 10% formamide in 2X SSC before washing for 5 min with PBS and mounting of the coverslips using Vectashield (Vector Laboratories).
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bioRxiv - Immunology 2021Quote: ... slides were washed 5 times with 1X PBS prior to being mounted with VectaShield medium containing DAPI (Vector lab, Burlingame, CA).
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed an additional 3 × 5 minutes with PBS before adding mounting medium containing DAPI counterstain (Vector labs, H-1200) and applying glass coverslips ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were washed three times in PBTx for 15 min followed by five 5-minute washes in PBS and mounted in Vectashield antifade mounting medium containing DAPI (Vector Laboratories). Samples were imaged on an Olympus FV3000 Confocal Laser Scanning Microscope and processed in Imaris.
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 6 separate times in TBS-T for 5 min and then incubated with HRP-conjugated streptavidin (Vector Laboratories) for an additional hour ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the fixed specimens were washed thrice for 5 min in 1x phosphate buffered saline (1xPBS; pH 7.4) and mounted using Vectashield mounting medium (H-1000, Vector Laboratories, CA). FITC-albumin and 10 kD dextran specimens were imaged using a ZEISS LSM900 confocal microscope with ZEISS Zen acquisition software (blue edition ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed 3 x 5 mins in PBST and incubated with fluorescein isothiocyanate-conjugated streptavidin (1:50; Vector Laboratories) in blocking buffer for 30 mins at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and incubated with anti-mouse secondary (1:50, Texas Red; Vector Laboratories) for 2 h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 x 5 mins in PBST and mounted onto glass slides with DAPI nuclei stain VECTASHIELD mountant (Vector Laboratories). Samples were visualised on Evos Cell imaging system (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 0.5 μg/mL DAPI in PBS for 5 min prior to drying with ethanol and were mounted using Vectashield mounting medium without DAPI (Vector Laboratories). Images were acquired and analysed using a Leica fluorescence microscope ...
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bioRxiv - Neuroscience 2023Quote: ... The CST was labeled using the Avidin Biotin Complex method (ABC) to detect the BDA by incubating in ABC (5 µL/mL A and B; PK-6100; Vector Laboratories) overnight at room temperature and co-labeled against GFAP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea) simplicifolia lectin 1 (5 μg/ml) (Vector Laboratories; L-1100). OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα ...
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 immunohistochemistry (IHC) was performed on 5 μm sections using a mouse on mouse immunodection kit (Vector Laboratories BMK-2202). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation with the biotinylated secondary antibody (1:150, Vector in 5% NGS in PBST) for 1 h was followed by the Vector ABC Kit (Vector Laboratories, solution A and solution B in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were washed twice in PBS (5 min) and mounted using Vectashield without DAPI (Vector Laboratories Inc., Burlingame, CA, USA) for immediate visualization by microscopy.
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bioRxiv - Pathology 2023Quote: ... The sections were washed with PBST (3x, 5 min) and developed using a DAB substrate kit (Vector Laboratories, Burlingame, CA, USA). Counterstaining was performed with hematoxylin ...
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bioRxiv - Biochemistry 2022Quote: ... Fixative was washed out with a continuous flow of 50 μl blocking buffer (5% normal goat serum S1000 in low-salt CSFxB; Vector Labs), then reactions were incubated in that buffer for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Organs were washed three times in PBS for 5 min per wash and mounted in Vectashield (Cat# H-1000, Vector Laboratories).
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... 250 μM CuSO4 and 2.5 mM sodium ascorbate were mixed as a reaction pre-mix with 100 μM of alkyne-label: TAMRA-alkyne (Vector Labs), biotin-PEG4-alkyne (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again washed three times 5 min and then mounted with Vectashiled HardSet Antifade Mounting Medium (#H-1400, Vector Laboratories) on gelatin-coated slides ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 min of running water before antigen retrieval in Tris EDTA buffer pH 9.0 (Vector Laboratories H-3301-250) or Citrate-based pH 6.0 (Vector Laboratories H-330-250 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were shortly stained with 5 µg DAPI (Applichem A1001) and mounted on glass slides using Vectashield (Vector Labs H-1000). The distribution of Flag-eIF2A was analysed on a confocal microscope (Leika TCS SP8 ...
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bioRxiv - Neuroscience 2024Quote: ... After 3 washes in PBS cells were incubated with a blocking medium consisting of (5% (w/v) bovine serum albumin (Vector Laboratories) and 0.2% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... samples were cleared in 70% glycerol in PBS for 5 min at room temperature and then mounted in Vectashield (Vector Labs). Confocal microscope images were taken under 10x or 63x magnification using a Zeiss LSM800.
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed three times in PBST-PVA for 5 min and mounted in Vectashield with DAPI (H-1200-10, Vector Laboratories) under #1 coverslips supported by Dow Corning high-vacuum silicone grease (Z273554-1EA ...
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bioRxiv - Neuroscience 2020Quote: ... filled with a chloride solution 0.5 M containing neurobiotin tracer (1-2%, w/v; CAT#SP-1120, Vector laboratories) to perform juxtacellular labelling ...
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bioRxiv - Neuroscience 2021Quote: Reagents used were as follows: N-(2-aminoethyl) biotin amide hydrochloride (NEUROBIOTIN™ Tracer, SP-1120, from Vector Laboratories), Alexa 488-conjugated streptavidin (Molecular Probes ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then incubated at room temperature for 2 h in biotinylated goat anti-mouse (1:200, Vector Laboratories). Brain sections were further processed using the Avidin-Biotin Complex (ABC Elite kit ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 2 h incubation in a solution containing avidin-biotin-peroxidase complex (ABC, 1:300 dilution, Vector Laboratories). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 2 h incubation in a solution containing avidin–biotin–peroxidase complex (ABC, 1:300 dilution, Vector Laboratories). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 h at room temperature with species-specific biotinylated secondary antibody (1:200 dilution) (Vector Laboratories) in 0.1M PBS ...