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Citations for Vector Labs :
601 - 650 of 1982 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 × 5 min in PBS followed by blocking in 20% normal goat serum (NGS; Vector Labs S-1000, Burlingame, CA, USA) in PBS for 1 hr at RT ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for monoclonal antibodies; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for polyclonal antisera and monoclonal antibodies, respectively; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then washed 3 times and treated with avidin-biotin complex solution (VECTASTAIN® Elite ABC-HRP Kit, Vector Lab, #PK-6100) for 90 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Following one (cell lines) or 3 days expression (primary hippocampal cultures) cells were fixed using paraformaldehyde and coverslips mounted using Vectashield (Vector Laboratories, Burlingame, CA, USA) with or without DAPI (cell lines) ...
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bioRxiv - Neuroscience 2023Quote: ... then reacted for visualization of peroxidase with nickel-cobalt intensified diaminobenzidine for 3 - 8 minutes (DAB Peroxidase substrate kit SK-4100, Vector Laboratories, Burlingame, CA, USA). Brain slices were washed between each incubation step using TBS (3 × 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... cells were washed 3 times with PBS on ice and bound biotinyl tyramide was visualized with fluorescent streptavidin (Vector Laboratories, Inc., Burlingame, CA, USA) at 1:1000 in PBS for 30 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissues were then washed 3 times for 5 minutes each in PT and mounted in Vectashield with DAPI (Vector Laboratories; H-1200-314 10).
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bioRxiv - Cell Biology 2021Quote: ... 25 µL of 1 mg·mL−1 avidin DN (Vector Laboratories, Burlingame CA) was introduced ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 hour with secondary antibodies (1:100, Vector Labs BA-9200), with 3×10 min washes after each incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% Mouse on Mouse Blocking Reagent (Vector Laboratories, MKB-2213-1)) for 30 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... beads were stored at 4°C in PBS to reduce background before mounting in VECTASHIELD antifade mounting media (Vector Laboratories H-1000). Images were acquired on a confocal laser scanning microscope (Leica SP5) ...
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bioRxiv - Biophysics 2021Quote: ... The cells were subsequently fixed in 4% paraformaldehyde for 15 minutes and the coverslips were mounted with Vectashield + DAPI (Vector Laboratories, USA) on a glass slide ...
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bioRxiv - Neuroscience 2022Quote: ... Delipidated sections in PBS were blocked with BSA 4 % and incubated with a 50 ug/ml solution of lectin conjugated with fluorescein (Vector Laboratories, FL1171), 24 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2 % Tween-20 in PHEM buffer and mounted using Vectashield mounting media (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were washed 4 times with PBST and mounted using VectaShield Hardset Antifade mounting medium (Vector Laboratories, Cat#H-1700-10). Images were taken using a Keyence BZ-810 all-in-one fluorescence microscope or a Zeiss LSM 700 inverted confocal microscope.
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bioRxiv - Cancer Biology 2024Quote: ... They were then blocked with 10% goat serum in PBS-Triton 0.1% (T-PBS) containing 4 drops/ml of Avidin solution (of the Avidin/Biotin Blocking Kit, Vector Labs SP-2001) for 1 hour and then incubated overnight with the primary antibody (Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... De-waxed and rehydrated sections were heated up and then pressure cooked for 4 minutes in antigen unmasking solution (H-3300, Vector Laboratories Inc). Once cooled ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... UEA-1 (Vector Laboratories), MerTK (2B10C42) ...
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bioRxiv - Immunology 2024Quote: ... UEA-1 (Vector Laboratories), and detected with Alexa 568 (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... 1% Neurobiotin (Vector Laboratories) was added to the internal solution ...
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bioRxiv - Neuroscience 2024Quote: ... 3 x 10min PBS washes were followed by mounting in VECTASHIELD® HardSet™ mounting medium with DAPI (Vector Labs, UK, Cat# H-1500-10).
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS 3 more times (10 min, room temperature) and mounted to microscope slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, cat. # H-1200-10). Sections were imaged on a Nikon A1R confocal microscope at the Tufts University Center for Neuroscience Research Microscopy Core ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 1 h incubation with an avidin-biotin-peroxidase complex (1:200, 1 h; Vectastain ABC Kit, Vector Laboratories). The immunochemical reaction was developed in a diaminobenzidine (DAB)-nickel solution containing 0.02% DAB ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies were incubated for 1 hour in 1% BSA/PBS along with the fluorescein labelled 1:4000 Vicia villosa lectin (VVL) (Vector Lab) or 1:1000 Helix pomatia agglutinin (HPA ...
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bioRxiv - Neuroscience 2024Quote: ... made in horse, 1:250; anti-mouse made in horse, 1:250; anti-rabbit made in horse, 1:250, Vector Laboratories). Then ...
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bioRxiv - Neuroscience 2020Quote: ... and UEA-1 (1:200, directly conjugated to FITC or Rhodamine, Vector Labs). Following primary antibody incubation and washing ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Neurobiotin 488 in 1 M NaCl (Vector laboratories, product SP-1125-2), or 0.1 % Fluoro-Gold in saline (Fluorochrome ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated secondary labeling (8 hr; dilution 1:100, Vector labs, USA; Table 1) was followed by tertiary labeling with avidin-biotin complex (Vector labs ...
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bioRxiv - Biochemistry 2023Quote: ... UEA DyLight 649 labeled Ulex europaues Agglutinin-1 (UEA, 1:200, Vector Labs), EdU (ClickIT EdU ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were rinsed 3 times for 10 minutes in TBS-T and then incubated for 1 hour in 1% bovine serum albumin TBS-T containing secondary biotinylated antibodies (1: 200; Vector Laboratories Inc.). Following the secondary antibody incubation step ...
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bioRxiv - Cancer Biology 2022Quote: ... or an anti-c-MYC antibody (1:100, 5h; Epitomics, #P01106; secondary: biotinylated goat anti-rabbit IgG, 1:200, 1 h; Vector labs, #PK6101). Blocker D ...
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bioRxiv - Neuroscience 2023Quote: ... the brain sections were incubated with secondary antibodies for 1 hour in 1% bovine serum albumin (BSA) containing TBS-T (1: 200; Vector Laboratories Inc.). Following the washing step with TBS-T ...
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bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fixed with 4% paraformaldehyde and stained with 10 mg/mL rhodamine-labeled Griffonia simplicifolia lectin I (GS-1lectin; Vector Laboratories; Burlingame, CA). Five randomly selected fields were imaged per gel to an area of 3×3 mm and a depth of ∼500 µm using a Zeiss LSM 700 confocal microscope ...
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bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures were subsequently fixed in 4% PFA and stained for ALP using the Vector Blue Alkaline Phosphatase Substrate Kit (Vector Laboratories SK-5300) and Von Kossa using a 4% silver nitrate solution and exposing cultures to 2400 kJ of ultraviolet light using a UV Stratalinker.
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bioRxiv - Neuroscience 2024Quote: ... An autofluorescence quenching solution was created by making a 1:1:1 solution of reagents in the Vector® TrueVIEW® Autofluorescence Quenching Kit (SP-8400-15, Vector Laboratories). This solution was added on top of the samples and incubated for 3.5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with phospho-S6 antibody for 1 hour (1:200, CST, #4858) followed by biotinylated anti-rabbit secondary antibody (1:100, Vector Labs, #BA-1000) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... IB4 (1:200, Vector Laboratories), anti-Ki67 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... and UEA-1 (Vector Laboratories). Images were analyzed with a TCS SP8 confocal laser scanning microscope (Leica).
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bioRxiv - Evolutionary Biology 2023Quote: ... biotinylated (1/200; Vector Laboratories) for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... UEA-1 (ZC0426, Vector Labs), CD8a (100714 ...
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bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...