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Citations for Vector Labs :
551 - 600 of 1982 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections were first washed with a series of PBS and 0.5% PBS-Triton X-100 (PBS-T) before applying a 3% normal goat serum (NGS, #1000, Vector Laboratories, Burlingame, CA) blocking solution for 1 hour at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... Elution was done in 4 bed volumes with corresponding elution buffers (Glycoprotein Eluting Solution, Cat. No. ES-3100 Vector labs for AAL column and 0.1M Melibiose monohydrate for GSI Cat# AK-2401-2) ...
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bioRxiv - Cancer Biology 2021Quote: ... IHC was performed on 4-μm sections with IHC was performed using Avidin/Biotin Blocking Kit (Vector Laboratories, SP-2001), Avidin-Biotin Complex kit (Vector Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... the sections were incubated with blocking buffer (PBS, 0.25% Triton-X-100, 10% goat serum, 4 drops/ml streptavidin (Vector Laboratories)) ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed and stained for 30 min at 4°C with 5 μg/ml FITC- WGA (Vector Laboratories). After the wash ...
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bioRxiv - Biophysics 2024Quote: ... The co-culture was incubated overnight at 4 °C with 10 µg/ml of Tomato Lectin (Vector Laboratories, #DL-1178) in blocking solution ...
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bioRxiv - Biophysics 2023Quote: ... The co-culture was incubated overnight at 4 °C with 10 mg/ml of Tomato Lectin (Vector Laboratories, #DL-1178) in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... then 4 times 20 min in PBS at RT before leaving brains overnight in Vectashield (Vector Laboratories H-1000-10) at 4C ...
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bioRxiv - Genomics 2023Quote: The aldehyde groups resulting in a 40 μl mix were then biotinylated using a mixture containing 4 μl of NaOAc (1M, pH 6.0) and 4 μl of Biotin (Long Arm) Hydrazide (100 mM, catalog num. SP-1100, Vector Laboratories). The 48 μl resulting mixture was next incubated for 30 minutes at 40°C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was completed for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
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bioRxiv - Pathology 2024Quote: ... or anti-mouse (1:2,500, PI-2000-1, Vector Labs) secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... 1:40 Mouse on Mouse (Vector Laboratories; MKB-2213-1), syringe filtered ...
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bioRxiv - Cell Biology 2020Quote: ... The coverslips with cells were mounted on 3 cm glass bottom cell culture plates using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Random fields on the coverslips were photographed using an inverted Olympus IX81 microscope (Olympus Europe Holding Gmbh ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Sections were again washed 3×15 min with PBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) (2-hours ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in 3 x 5 min in TBST and mounted with Vectashield® mounting medium (Cat. No. H-1000; Vector Laboratories, USA).
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with PBS and incubated for at least an hour with blocking buffer containing 3% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The proximal end of the femur was first dipped in phosphate-buffered saline with 0.5% Triton-X (PBST) for 2-3 seconds and immediately transferred to a Vaseline® well of neurobiotin 488 tracer (Vector Labs, USA) solution prepared in 1M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 times with PBST for 5 min each and mounted with Vectashield vibrance with DAPI (Cat. # H-1800-10, Vector Labs, Newark, CA). Cells were imaged at 63x on the confocal 60 μm disk setting with the BioTek Cytation C10 using the Gen5 software (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... and after 3 more washes with PBS the coverslips were mounted with Vectashield solution (mounting medium with DAPI, Vector Laboratories Inc., Burlingame, CA). Evaluation was done using a Nikon 80i or Bio-rad confocal microscope ...
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bioRxiv - Neuroscience 2022Quote: ... and UEA-1 594 (1:1000; Vector Laboratories, Cat# DL-1067), in antibody diluent (Dako ...
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bioRxiv - Cancer Biology 2022Quote: ... and Tomato Lectin Biotinylated (1:250, Vector Laboratories, B-1175-1) for one hour at room temperature followed by APC-Streptavidin secondary (1:500 ...
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bioRxiv - Pathology 2024Quote: ... incubated in anti-rabbit (1:2,500, PI-1000-1, Vector Labs) or anti-mouse (1:2,500 ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-rabbit (PI-2000-1, PI-1000-1; Vector Laboratories, respectively), or anti-guinea pig (61-4620 ...
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bioRxiv - Genetics 2020Quote: ... Sections were incubated with primary antibody (supplementary table S4) overnight at 4°C and with universal biotinylated horse anti-mouse/rabbit immunoglobulin G secondary antibody (Vector Laboratories) for 1 hour ...
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bioRxiv - Physiology 2021Quote: ... Ears were then gently shaken (120 rpm) at 4°C overnight in 20 μg/ml Dylight 488 strepavidin (SA-5488, Vector Laboratories) and 0.05 mg/ml DAPI (D9542 ...
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bioRxiv - Physiology 2021Quote: ... Frozen bone sections were incubated for 4 min at room temperature in Alkaline Phosphatase Substrate Solution ImmPACT Vector Red (Vector Laboratories). After washing with buffer ...
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bioRxiv - Microbiology 2021Quote: ... ovaries were fixed in 4% PFA and then mounted in Vectashield® mounting media with DAPI counterstain (Vector Laboratories, Burlingame, CA). Ovaries were then imaged using Differential Interference Contrast on a Zeiss Inverted Observer Z1 at 100X magnification.
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bioRxiv - Neuroscience 2021Quote: ... Slides were blocked with 10% normal goat or horse serum followed by overnight incubation with primary antibody at 4°C and 30 min incubation with ImmPRESS HRP-conjugated secondary antibodies (Vector Laboratories) at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with primary antibodies at 4°C overnight followed with biotinylated HRP-conjugated secondary antibody (Vector Laboratories # BA-1000) at room temperature for 1 hour ...
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bioRxiv - Physiology 2024Quote: ... was incubated at 4°C overnight and a biotinylated secondary antibody followed by streptavidin-DyLight 594 (BA1000 and SA-5594, Vector Laboratories) were used for cardiomyocyte identification ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated first with a Biotinylated Goat Anti-Rabbit IgG Antibody (over-night at 4°C-Vector Laboratories), and then with Alexa Fluor™ 594 streptavidin (3 hours – room temperature-Thermo Fisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... The polyps were kept for at least o/n at 4°C in a Vectashield mounting medium with DAPI (Vector Laboratories) before being mounted and imaged on a Leica SP8 confocal system ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2% Tween-20 in PHEM buffer and mounted in Vectashield (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibodies for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an hour in Mouse-on-Mouse (MOM) Blocking Buffer (one drop MOM IgG in 4 ml PBS-T) (Vector Labs). Following the MOM block ...
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bioRxiv - Neuroscience 2024Quote: ... was followed by incubation with the primary antibody (Table S2) at 4°C for 24 or 48h in 2% (vol/vol) serum and with the corresponding biotinylated antibody (Vector Laboratories) or alkaline-phosphatase antibody (ab6722 ...
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bioRxiv - Systems Biology 2024Quote: ... the sections were washed again in PBS-T and incubated in Mouse-on-Mouse (MOM) Blocking Buffer (one drop MOM IgG in 4 ml PBS-T) (Vector Labs) for another hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies for 48 h at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 h at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were again washed 4 times with PBS-T followed by addition of avidin D-horseradish peroxidase (HRP) (Vector Laboratories) diluted 1:1,000 in PBS-T with 1% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-PU.1 (1:200, 2258S, Cell Signalling, UK) and biotinylated RCA1 (1:1000, B10855, Vector Labs, UK). Mouse anti-Ki67 (1:400 ...