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Citations for PerkinElmer :
251 - 300 of 379 citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Mice were imaged for 2 minutes using a cooled charged-coupled device camera (IVIS Lumina; Perkin Elmer, Coventry, UK). Dams were anaesthetised with isoflurane as above.
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bioRxiv - Developmental Biology 2023Quote: ... ORO was measured by extracting lipids with a petrol ether/isopropanol mixture (3:2) and quantified for their absorbance at 490 nm for 0.1 s with Victor Spectrophotometer (PerkinElmer). Standard curve was applied and quantification was expressed in µg of pure ORO powder.
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bioRxiv - Bioengineering 2023Quote: ... FTIR of CNPs was measured in ATR (attenuated total reflectance) mode using a Spectrum 2 FTIR spectrometer from PerkinElmer. The scanning range for the measurements was 1000 cm-1 to 4000 cm-1.
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bioRxiv - Physiology 2023Quote: ... Measurement of radiolabelled 2-deoxyglucose was carried out in a Tri-Carb 4910 TR liquid scintillation analyzer (Perkin Elmer) by adding 200 μl of muscle lysate to 4 ml scintillation fluid (Ultima Gold ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
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bioRxiv - Immunology 2024Quote: ... Tumor progression was subsequently monitored every 2-3 days using caliper measurement and the IVIS Spectrum imaging system (PerkinElmer) to measure bioluminescent signal after intraperitoneal administration of 0.15 mg of luciferin substrate per gram of body weight (PerkinElmer 122799) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 24-well plates for 2 h with 0.25 ml/well of fresh growth medium containing 5 μCi/ml [3H]adenine (PerkinElmer). The medium was then replaced with 0.5 ml/well of DMEM containing 20 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... 50% and 25% MIA PaCa-2 cells) were grown in CellCarrier Spheroid ULA 96-well Microplates (6055330, PerkinElmer, Waltham, MA), as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... the Mia PaCa-2 cells and PCAFs were seeded in CellCarrier Spheroid ULA 96-well Microplates (6055330, PerkinElmer, Waltham, MA) with 0% ...
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bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...
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bioRxiv - Plant Biology 2021Quote: ... leaf discs excised from the transformed leaves were incubated with 200 µM D-luciferin (Wako) for 2 h in white 24-well plates (PerkinElmer). Then ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated in the dark for 2 h at RT before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of FITC 485 / FITC 535 ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Microbiology 2019Quote: ... was achieved by addition of GDP- or UDP-GloTM nucleotide detection reagent following manufacturers’ instructions and analyzed via luminescence using a Victor 2 microplate reader (PerkinElmer).
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bioRxiv - Biophysics 2019Quote: ... After sharing at 600 rpm for 2 h at room temperature the plate transferred to a MicroBeta2 scintillation counter (PerkinElmer) left for 4 h to allow the SPA-beads to settle before quantifying 125I radioactivity ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
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bioRxiv - Biochemistry 2021Quote: ... 50 nM of hNatD was mixed with 2 μM of radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences), 20 μM of the peptide ...
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bioRxiv - Biochemistry 2020Quote: ... was counted using a PerkinElmer Tri-Carb 2800RT liquid scintillation counter after dissolving the filter in 2 ml of scintillation liquid (Emulsifier Scintillator Plus, PerkinElmer).
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bioRxiv - Cell Biology 2021Quote: ... buffer and 1.2 M sorbitol citrate solution, and then digested by 1.4 mg/ml 20T zymolyase (amsbio, 120491) and glusulase (PerkinElmer, NEE154001EA). Cells were spread on poly-L-lysine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... we used initially the RNAscope Multiplex Fluorescent Assay version 1 with the fluorophores Alexa 488 and Atto 550 and in later experiments the RNAscope Multiplex Fluorescent Assay version 2 with the fluorophores Opal 570 and Opal 650 (PerkinElmer) according the manufacturer’s protocol for multiplex fluorescent in situ hybridization kit ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... 2-deoxyglucose (2DG) uptake in tissues in vivo were measured by the retro-orbital injection of [3H]2DG (Perkin Elmer) diluted in saline ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were acquired with an AxioObserver 2 mot plus fluorescence microscope (Zeiss, Munich, Germany) or with an Operetta high- content imaging system (PerkinElmer) at 5x magnification ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then washed with Krebs-Ringer bicarbonate HEPES (KRBH) buffer and then given 400 μL with KRBH buffer loaded with 0.8 μCi/well [3H]-2-deoxyglucose (PerkinElmer, NET549A001MC) and 0.5 mM unlabeled 2-deoxyglucose and incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Cell Biology 2019Quote: GBM2 cells were plated at 4000 cells/well and exposed to epigenetic compounds (Table 2) at 10 µM for 1 day in 384-well optical bottom assay plates (PerkinElmer). Negative control was DMSO (0.1%) ...
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bioRxiv - Genetics 2019Quote: ... PRMT7 wild-type and mutants were incubated with putative substrates in PBS with 2 μCi of S-adenosyl-[methyl-3H]methionine ([3H]AdoMet) (55-85 Ci/mmol, PerkinElmer) for 18 h at 26°C ...
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bioRxiv - Microbiology 2019Quote: ... and 500 µl were transferred to 2 ml Eppendorf tubes and spiked with 2.5 µCi of 32P-orthophosphoric acid (Perkin Elmer). The cultures were grown for two generations (OD600 0.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... was next added to the cultures and cells were labeled for 2 min with [2,8-3H]-adenine (NET06300 Perkin Elmer) at 0 ...
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bioRxiv - Bioengineering 2020Quote: Bioprinted SK-N-BE(2) spheroids were prepared as described earlier in a 96-well plate (Cell-Carrier, Perkin Elmer). After incubating the plate for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated in the dark for 2 h at room temperature before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer), as described previously74 ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 pmole trypSL (1.25 µl from a 50µM stock was kinased with 2 µl 32PgammaATP (25 µM, 6000Ci/mmol, 150 mCi/ml, Perkin Elmer) with 10 U PNK in 10 µl with 20 U RNasin (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... TZM-bl reporter cells were seeded at a density of 2 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Microbiology 2023Quote: ... A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... The bioluminescent signal emitted by the disseminated tumors was measured at 7-day intervals for 2 months using the IVIS Spectrum In Vivo Imaging System (PerkinElmer). The weight of the mice was recorded before each measurement.
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 12 hr following TNFα addition using the Opera Phenix Plus High-Content Screening System (PerkinElmer), with two biological replicates (n = 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 48 hr following TNFα addition using the Opera QEHS imaging system (PerkinElmer). Nuclear RELA and PCNA intensities were measured using Nuclitrack software (Cooper et al. ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 25-45 images were acquired on an Opera Phenix high-content imaging system (objective 20x, z-stacks of 2 μm, PerkinElmer). Image analysis was performed using Harmony imaging analysis software (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase measurements were taken every other day beginning from day 2 (the day after the injection) – mice were anaesthetised (isoflurane inhalation) and subcutaneously injected with Xenolight D-luciferin substrate (PerkinElmer). Mice were imaged after 5min using the IVIS Lumina II (Caliper Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Physiology 2024Quote: ... or 120 nM of insulin in fresh serum-starvation medium for 30 min before adding 2 μL.mL-1 D-[14C(U)] glucose (NEC042B005MC; Perkin Elmer) for a further 90 min ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence polarization measurements were carried out in black 96-well plates measured on a Wallac Victor 2 Plate Reader (Perkin Elmer). For all measurements peptide was held at concentrations of 50 nm (or 100nM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...