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Citations for PerkinElmer :
1 - 50 of 379 citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
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bioRxiv - Microbiology 2020Quote: ... All virus supernatant was quantified via p24 ELISA (Perkin Elmer).
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... A fraction (50 μl) of the lysate was dissolved in 2 ml of β-scintillation liquid (Ultima Gold, Perkin Elmer) for measurement of 2-DG transport using [14C]mannitol to estimate extracellular space using β-scintillation counting ...
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bioRxiv - Immunology 2020Quote: ... Serum-virus mixtures were then transferred to 96-well plates (PerkinElmer), and 1.0×104 MDCK-SIAT1-PB1 cells51,92 were added into each well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... pseudotyped virus treated hESC-derived cardiomyocytes in CellCarrier-96 Ultra Plates (PerkinElmer) were washed 3x with 100 μL PBS before complete submersion in 2-4% formaldehyde solution for 30 mins ...
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) and by real time PCR ...
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bioRxiv - Microbiology 2020Quote: ... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) or by qPCR ...
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Microbiology 2019Quote: ... WSN-GFP virus expression was detected by the Operetta-High Content Imaging System (PerkinElmer Inc.), and the percentage of GFP-positive cells were analyzed by Harmony4.1 software (PerkinElmer Inc.) ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi) ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was mixed with Ultima Gold (Perkin Elmer) and the relative expression of Na+-dependent uptake of radiolabeled glutamate was calculated based on the measurement with a microplate scintillation counter (1450 MicroBeta Plus ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoblots were visualized with either Supersignal West Femto (Pierce) or Western Lightning Plus ECL (PerkinElmer). Image quantification analysis (ImageJ ...
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bioRxiv - Genetics 2022Quote: ... SuperSignal™ West Pico PLUS (34580, Thermo) and Western Lightning Plus-ECL (NEL104001EA, Perkin Elmer) were used as chemiluminescent substrates ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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bioRxiv - Microbiology 2023Quote: ... Virus concentration was estimated by p24 titration (HIV-1 alliance p24 ELISA kit, PerkinElmer, Cat. #NEK050001KT).
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bioRxiv - Immunology 2020Quote: ... The virus infectivity titers were determined by measuring the luciferase activity using Britelite luciferase substrate (PerkinElmer Inc.) in a luminometer (Victor X2 ...
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bioRxiv - Microbiology 2022Quote: ... the virus was identified using liquid scintillation analyzer TRI-carb 2910 TR (Perkin Elmer, Waltham, Massachusetts, US). The virus containing fractions were collected and pelleted as earlier ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were placed into a white 96-well plate (Perkin Elmer) to assess Luciferase and Renilla activities using Envision Multilabel plate reader (Perkin Elmer) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was transferred to light grey 384-well untreated AlphaPlates (PerkinElmer) by adding 10 µl per well ...
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bioRxiv - Biochemistry 2023Quote: ... and the remaining lysate was mixed with Optiphase HiSafe 3 (PerkinElmer). The radioactivity was monitored using a β-scintillation counter (LSC-8000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen-antibody complexes were detected with West Pico Chemi luminescent substrate using standard manufactures protocol (Perkin Elmer). Imaging of all western blots was performed using an UVP BioImager 600 system equipped with VisionWorks Life Science software (UVP ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell lysates were combined with 8mL Ultima Gold scintillation cocktail (PerkinElmer 6013329) and CPM was measured using Beckman LS6500 Scintillation counter ...
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bioRxiv - Microbiology 2022Quote: ... ESAs and lysates were plated in white-bottom assay plates (PerkinElmer #6002290) and incubated at room temperature for 5 min with substrate-containing assay solution before detecting luciferase signal using a Biotek Cytation3 multimode reader ...
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bioRxiv - Immunology 2021Quote: ... The luciferase signal was determined in live animals one day after virus injection and 10 minutes after intraperitoneal injection of 150 mg/kg D-luciferin (PerkinElmer) using the in vivo imaging system NightOWL II LB 983 (Berthold ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were transferred to a 384 OptiPlate (Perkin Elmer, Waltham, MA, USA) and mixed 1:1 with antibody mixture from the IP-ONE-Gq kit ...
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bioRxiv - Biochemistry 2019Quote: ... cell lysate (5 μl/well) was transferred to a 384-well ProxiPlate (PerkinElmer) and added to the donor and acceptor reaction mix (2.5 μl/ well ...
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bioRxiv - Microbiology 2022Quote: ... Cells were imaged and percent of virus infected cells calculated using the Operetta High Content Imaging System and Harmony® High Content Imaging and Analysis Software (PerkinElmer).
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 110 μl of the cell lysate was transferred to a black/white plate (PerkinElmer). Luminescence was measured using a PerkinElmer Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Four μL of cell lysate was transferred to a 384 well ProxiPlate (Perkin Elmer). Immediately prior to use ...
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bioRxiv - Molecular Biology 2022Quote: ... Luminescent signals were generated after incubation with SuperSignal® West Pico Chemiluminescent substrate (Pierce Biotechnology) and captured with Kodak Biomax X-ray film (PerkinElmer).
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bioRxiv - Immunology 2020Quote: ... The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2, PerkinElmer Life Sciences).
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bioRxiv - Neuroscience 2021Quote: ... 10 µl of lysates were manually transferred to a 384-well opaque OptiPlate (Perkin Elmer) in quadruplicate wells and supplied with APC-POM1 and EU-POM2 (5 and 2.5 nM final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... using 10µl lysate in a black OptiPlate 96-well plate (6005279, Perkin Elmer, Waltham, MA) and 50 μL homemade firefly and luciferase reagents (according to (Fuerer et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cell lysate (5 μL/well) was transferred to a 384-well ProxiPlate (PerkinElmer, Melbourne, Australia) and added to the donor and acceptor reaction mix (2.5 μL/ well ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were incubated with 1 mM GTP/[α-32P]GTP (0.1 μCi/μl) (PerkinElmer) and incubated at 30°C for various time intervals for comparison pYfiN and pYfiN(Ips) ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Equal volumes of lysate were then subjected to analysis using an EnSpire Multimode Plate Reader (PerkinElmer).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 μL of the cell lysates were transferred into a white 384 well plate (ProxiPlate, PerkinElmer). 5 μL of the Europium-cAMP tracer (1 to 50 dilution of Eu-tracer stock in cAMP detection buffer ...
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bioRxiv - Physiology 2023Quote: ... by adding 200 μl of muscle lysate to 4 ml scintillation fluid (Ultima Gold, Perkin Elmer). Glucose uptake rates were calculated as described in 14.
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bioRxiv - Physiology 2024Quote: ... by adding 200 μL of muscle lysate to 4 mL scintillation fluid (Perkin Elmer, Ultima Gold). Glucose uptake rates were calculated as described previously 20.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer), and the other half was used for immunoblotting.
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL cell lysate was added to 3 mL of Ultima Gold scintillation liquid (Perkin Elmer #6013329). Samples were kept in the dark overnight and 3H-2DG uptake was measured the following day on a Hidex 300 SL scintillation counter ...
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bioRxiv - Physiology 2024Quote: ... 0.5 mL of the cell lysate was counted in a liquid scintillation counter (TRI-CARB 4910TR, PerkinElmer) and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... 2’-14C]-cystine (PerkinElmer) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Absorbance of the lysates was measured at 420 nm using a spectrophotometer (EnSpire Multimode Plate Reader, Perkin Elmer).
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were transferred into vials and measured using a gamma counter (Wallac 1470 Wizard, PerkinElmer, Waltham, MA, USA). The saturation assay was performed by adding 100 μL of binding buffer with increasing amounts of 125I-NDP-MSH to the cells in the presence or absence of 1 μM of unlabeled NDP-MSH.
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was quantified by measuring luciferase activity in cell lysates using Britelite Plus (Perkin Elmer, Richmond, California) and following the manufacturer’s protocol ...