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Citations for PerkinElmer :
251 - 300 of 548 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed in PBS containing 0.1% Tween-20 and then exposed to developing solution (Western Lighting Chemiluminescence kit, Perkin Elmer) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of BSA, carnitine, and cold palmitic acid) with [9,10-3H]-palmitic acid (Perkin Elmer) for 6 hours ...
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bioRxiv - Genomics 2021Quote: ... and transferred to a plastic scintillation vial containing 10 mL of OptPhase HiSafe 3 scintillation cocktail (PerkinElmer, Waltman, MA, USA). All vials were counted for 10min using a Tri-Carb 2810 TR liquid scintillation analyzer (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Uptake was measured at room temperature by mixing 100 µl of cells with 100 µl of transport buffer containing 8 µM of [14C] glycerol (0.125 µCi each in triplicates, PerkinElmer Inc.). Incorporation of radioactivity was determined as described above.
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bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
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bioRxiv - Neuroscience 2022Quote: ... siScrambled or siAbdc1/2 Astrocytes were incubated overnight with DMEM without glucose supplemented with 1% FBS containing 1 μCi/mL 14C-Acetate ([1-14C] acetate, 58 mCi/mmol, PerkinElmer) and or α-cyclo or 50 μM C26:0 ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Cell Biology 2023Quote: ... On the next day transfer and mix of compounds into plates only containing cell culture media was performed with the Sciclone G3 Liquid Handling Workstation (PerkinElmer). This intermediate dilution step was included to avoid DMSO gradient effects on cell monolayers ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... media was replaced with fresh media containing 200 μM ascorbate and 100 mCi/mL of 35S-Cys/Met (Perkin Elmer) for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer] ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated secondary antibodies were from Perkin Elmer (NEF822001EA, NEF812001EA). Secondary antibodies conjugated to Alexa Fluor dyes were from Thermo Fisher Scientific.
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bioRxiv - Bioengineering 2022Quote: ... plates were incubated with anti-mouse IgG-Europium antibody (PerkinElmer) for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... secondary antibody goat anti-rat and Opal 520 (fp1487, PerkinElmer). After the third staining ...
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bioRxiv - Neuroscience 2022Quote: ... Flu-positive cells were visualized with anti-FITC antibody (PerkinElmer, #NEF710001EA ...
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bioRxiv - Developmental Biology 2022Quote: ... HRP-conjugated secondary antibodies and tyramide signal amplification kit (PerkinElmer) were used to magnify the signals ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were detected using Opal Polymer HRP (Perkin Elmer) and visualized using Tyramide Signal Amplification for 10 min (Opal 7-Colour IHC ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then pulsed with 2.20 MBq per sample with Express Protein Labeling Mix (Perkin Elmer) and incubated for 2.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and the radiolabeled proteins were detected and quantified using a Cyclone Plus Phosphor Imager system (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... Cultures were labeled with 80 µCi/mL [35S] EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer) for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and protein expression signals were detected using a chemiluminescence detection kit (Perkin Elmer, Waltham, MA, USA) and visualised using a chemiluminescence imaging system (Fusion Solo S ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labeled with 0.1 mCi/mL EasyTag EXPRESS 35S Protein Labeling Mix (Perkin Elmer) diluted in labeling media ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using Western Lighting Plus ECL detection reagent (Perkin Elmer, Waltham, MA, USA) and imaged with the ChemiDoc TouchTM system (Bio-Rad) ...
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bioRxiv - Genetics 2021Quote: Cells were fixed in growth medium with 3.7% formaldehyde at room temperature for 30 minutes and their cell walls digested with a solution containing 50 μg/ml zymolyase and 1:50 glusulase (Perkin Elmer). The digested cells were mounted on poly-L lysine coated slides to immobilize them for microscopy ...
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bioRxiv - Cell Biology 2021Quote: ... incubated at 37°C for 30 min in 500 μl starvation medium (DMEM lacking methionine and cysteine) and another 30 min in 500 μl labeling medium containing 100 μCi/ml [35S]methionine/cysteine (Perkin Elmer). The cells were washed twice with PBS and lysed in 500 μl lysis buffer (PBS with 1% Triton X-100 ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Neuroscience 2021Quote: ... was added to the chamber for 30 min to inhibit transcription after which slices were transferred to fresh aCSF containing ~10 mCi/ml [35S] Met/Cys (Perkin Elmer) for an additional 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in a solution containing an isotopic dilution of 2 μCi/ml [3H] glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Apoe-/- macrophages were isolated and incubated for 48 h with DMEM containing acetylated LDL (40 μg/ml) and 1.0 μCi/mL 3H-cholesterol (PerkinElmer, Boston, MA). The cells were then washed and incubated for 24 h in DMEM supplemented with HEPES in the presence of HDL or MDA-HDL ...
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bioRxiv - Biochemistry 2021Quote: ... Thirty μL of tissue homogenate supernatant were mixed with 370 μL of the oxidation reaction mixture containing 7% BSA/5 mM palmitate/0.01 μCi/μL 14C-palmitate (PerkinElmer, Waltham, MA) and incubated at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [5-3H]-glucose (Perkin Elmer) for 2-3 hours ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 12-well plates were incubated with 1 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [6-14C]-glucose (Perkin Elmer) for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 6-well plates were incubated with 2 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [14C(U)]-glutamine (Perkin Elmer) for 6 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... A spectral library containing the emitted spectral peaks of the fluorophores in this study was created using the Vectra image analysis software (Perkin Elmer). Using multispectral images from single-stained slides for each marker ...
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bioRxiv - Immunology 2022Quote: ... 50 μL of virus-antibody mixture was inoculated onto each well containing a Vero-E6 cell monolayer culture in 96-well plates (Cell Carrier Ultra plates, Perkin Elmer) that had been seeded the day before at 15,000 cells per well ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was started by incubating with 0.1 mM ATP containing 20 μCi of [γ-32P] ATP (3000 Ci/mmol; Perkin Elmer) containing 1 mM of cold ATP at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
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bioRxiv - Plant Biology 2023Quote: ... in the absence or presence of 1.5 mM CaCl2 with 100 μM non-radiolabelled ATP containing 370 kBq of [γ-32P]ATP (PerkinElmer; NEG002A) for 30 min at 25 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the SPOT arrays were incubated in methylation buffer containing additionally 50 nM SETD6 and 0.76 µM labelled [methyl-3H]-AdoMet (Perkin Elmer Inc.) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were initiated by the addition of GTP to a final concentration of 40 uM containing a trace amount of [alpha-32P]-GTP (Perkin Elmer) and incubated for 20 min at 30°C ...
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bioRxiv - Biochemistry 2024Quote: ... For competition binding experiments cell membranes expressing h5-HT3A WT or mutant receptors were incubated in 250 µL 10 mM HEPES buffer (pH 7.4) containing 0.3 nM [3H]-GR65630 (Perkin Elmer, Waltham, MA) and varying concentrations of VTX or analogues ...
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bioRxiv - Microbiology 2024Quote: ... The filters were rinsed with 35 ‰ sodium chloride (NaCl) solution [50] and transferred into 7 mL scintillation vials containing a 6 mL scintillation cocktail (Ultima Gold ™ Cocktail, PerkinElmer). The radioactivity of the filters was determined using a Tri-Carb 3110TR liquid scintillation counter [51] ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of heat-denatured DNA was incubated for 30 min at 37°C in a total volume of 25 μl containing random primer and 5 μl of [α-32P]-dCTP (3,000 Ci/mmol, 10 mCi/ml; Perkin Elmer). After the reaction ...
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bioRxiv - Immunology 2024Quote: ... 10 μl were transferred into a well containing 90 μl of SGal-URA of a 96-well optically clear flat-bottom plate (PerkinElmer 6055302). Data analysis of the high content imaging was performed in Fiji ...
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bioRxiv - Cell Biology 2020Quote: Posttranslational labeling was performed by incubating cells in normal growth medium containing 400 mCi/mL [3H]glutamine (PerkinElmer, 30-60 Ci/mmol) in the presence of (100 µg/ml ...
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bioRxiv - Immunology 2021Quote: ... 50μl supernatant was harvested from each well and placed in 96-well Luma Plates containing a dry scintillant (Perkin Elmer, Waltham, MA). Dried plates were counted on a MicroBeta counter (Perkin Elmer ...
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bioRxiv - Neuroscience 2020Quote: ... were harvested in Schneider’s medium on ice and transferred to Schneider’s medium containing 35S-methionine/cysteine (2 µCi/µl; Perkin Elmer Labs Inc., NEG709A) and 30 µM chloroquine (to inhibit autophagy) ...