Labshake search
Citations for PerkinElmer :
1 - 50 of 548 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: The excitation and emission spectra of the pure proteins diluted in PBS were recorded on an LS-55 fluorescence spectrometer from PerkinElmer. The emission spectrum of each protein was obtained by fixing the excitation wavelength at its maximum absorbance wavelength ...
-
bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
-
bioRxiv - Genetics 2019Quote: ... and 10-15µM 3HSAM tracer (55–75 Ci/mmol, PerkinElmer) and were incubated with 1µM Suv39/Clr4-SET and varying amounts of biotinylated H3(1–20 ...
-
bioRxiv - Biophysics 2022Quote: ... These assays were performed using LS 55 spectrofluorimeter (Perkin Elmer). Three consecutive spectra were averaged after blank correction for obtaining the final LS and ThT spectra.
-
bioRxiv - Biophysics 2022Quote: ... Fluorescence measurements were performed with a Luminescence Spectrometer (LS 55, Perkin Elmer). Fluorescence excitation spectra were recorded from 250–500 nm at a fixed emission wavelength of 496 nm ...
-
bioRxiv - Biophysics 2020Quote: ... These assays were carried out on a LS 55 spectrofluorimeter (Perkin Elmer). The blank was subtracted from consecutive spectra which were averaged to obtain the final LS and ThT spectra.
-
bioRxiv - Biophysics 2019Quote: Intrinsic tryptophan fluorescence was measured using a PerkinElmer LS 55 Fluorimeter (PerkinElmer, Inc., MA, USA). Excitation wavelength was 280 nm ...
-
bioRxiv - Bioengineering 2020Quote: Fluorescence spectroscopy was performed on 3HM-FAM solutions using a LS-55 fluorescence spectrometer (PerkinElmer). Stock solutions of FAM-labeled amphiphiles and unlabeled 3HM were prepared to 1 mg/mL in phosphate buffer (25 mM ...
-
bioRxiv - Bioengineering 2020Quote: Fluorescence spectroscopy was performed on 3HM-DOX solutions using an LS-55 fluorescence spectrometer (PerkinElmer). Stock solutions of 3HM-DOX were prepared ...
-
bioRxiv - Microbiology 2022Quote: ... in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
-
bioRxiv - Biochemistry 2024Quote: ... recording the fluorescence at 445 nm during 1 h on the LS-55 fluorescence spectrometer from PerkinElmer, using an excitation slit of 15 nm and an emission slit of 5 nm ...
-
bioRxiv - Biochemistry 2021Quote: ... 2020 submitted): namely in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
-
bioRxiv - Neuroscience 2020Quote: ... Plasma concentration of FD4 concentration was assessed using fluorescence spectrometer (LS 55 conducted with FL WinLab software, PerkinElmer, USA) at an excitation wavelength of 490 nm and emission wavelength of 520 nm.
-
bioRxiv - Neuroscience 2021Quote: ... Fluorescence was measured (excitation 360 nm, emission 450 nm) in glass cuvettes in luminescence spectrometer (Perkin Elmer LS 55), given as relative fluorescence units (RFU) ...
-
bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
-
bioRxiv - Cell Biology 2021Quote: ... Fluorescence of recombinant pHLuorin2 in each pH calibration buffer was measured using Eppendorf semimicro Vis Cuvettes on an LS 55 Fluorescence spectrometer (PerkinElmer). A xenon lamp was used as the excitation source ...
-
bioRxiv - Genetics 2019Quote: ... PRMT7 wild-type and mutants were incubated with putative substrates in PBS with 2 μCi of S-adenosyl-[methyl-3H]methionine ([3H]AdoMet) (55-85 Ci/mmol, PerkinElmer) for 18 h at 26°C ...
-
bioRxiv - Biophysics 2020Quote: Fluorescence emission was recorded in buffer (50 mM Tris-HCl pH 7.4, 50 mM KCl) on a fluorescence spectrometer (LS 55, Perkin Elmer) with excitation/emission slit width of 5 nm and gain values set to 775 V (PMT R928 ...
-
Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... 100 nM GBR12909 (to block binding to dopamine transporter) and 50 pM [125I]RTI-55 (NEX272, Perkin Elmer, Boston, MA) were added to the buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... Maximal projection was used to reconstruct representative Z-stack fluorescent confocal images of 25 fields with 30-35 Z-stack images spaced every 55 μm using the Harmony High-Content Imaging and Analysis Software (PerkinElmer). Maximal projection of all imaged fields and z-stacks was analyzed using ImageJ digital processing software (https://imagej.nih.gov/ij/ ...
-
bioRxiv - Microbiology 2022Quote: The lipid compositions of the bacterial strains were determined after labeling with [1-14C] acetate (specific activity: 55 mCi/mmol; PerkinElmer) or [35S] sulfate (specific activity ...
-
bioRxiv - Molecular Biology 2024Quote: ... the tryptophan fluorescence intensity of STNPDZ was measured in both the absence and presence of SPDZi1 using an LS 55 fluorescence spectrophotometer (PerkinElmer). The KD was calculated using the following equation ...
-
bioRxiv - Molecular Biology 2019Quote: Cells were incubated with complete growth media supplementing with EasyTagTM Express Protein Labeling Mix containing both [35S]-L-methionine and [35S]-L-cysteine (PerkinElmer) at 10 μCi/ml for 30 min at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: Polyacrylamide gel slices (1-2 mm) containing the purified proteins were prepared for mass spectrometric analysis using the Janus liquid handling system (PerkinElmer, UK). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6 ng/ µl recombinant TopBP1 protein or buffer were added to TopBP1 immunodepleted interphase Xenopus egg extract containing 50 nCi/µl α32P-dCTP (Perkin Elmer NEG513A250UC). To start the replication reaction the egg extracts were supplemented with 5 ng sperm DNA (kindly provided by the lab of O ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
-
bioRxiv - Biochemistry 2021Quote: ... This cell suspension was transferred into a cuvette and fluorescence was measured with a PerkinElmer LS 55 fluorimeter (Perkin Elmer, Waltham, MA, USA) under stirring conditions ...
-
bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
-
bioRxiv - Biophysics 2019Quote: ... The solubilized virus was diluted in HB buffer with 1% Triton-X and the fluorescence emission spectrum of the sample was measured using a fluorimeter (Perkin Elmer LS 55, Waltham, MA). The integrated fluorescence intensity across the spectrum was calculated and compared to a standard curve of known dye concentration in the same buffer to determine the concentration of fluorescent dye in the viral suspension ...
-
bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...
-
bioRxiv - Cell Biology 2021Quote: ... containing 35S methionine/cysteine (Perkin Elmer) in the presence of SP-cells at 30°C for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
-
bioRxiv - Physiology 2020Quote: ... and containing 0.5 μCi [14C]-acetate (PerkinElmer) and 5 μM sodium acetate for 12 h at either 37°C or 31°C ...
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in α-MEM containing media containing 2μM Glutamine and 4 mCi/mL L-[3,4-3H(N)]-Glutamine (PerkinElmer, NET551250UC). GLS activity was terminated by washing cells with ice cold HBSS for three times followed by scaping cells with 1 mL ice cold milliQ water ...
-
bioRxiv - Developmental Biology 2020Quote: ... A buffer containing Cy3 (PerkinElmer, Waltham, 1:50) was used to stain the samples for 40 min.
-
bioRxiv - Developmental Biology 2024Quote: ... containing 0.5% TSA blocking reagent (Perkin Elmer, FP1020) and Hoechst (Invitrogen ...
-
bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
-
bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... containing 6 ml Ultima Gold scintillation cocktail (Perkin Elmer). Radiation in each sample was measured as counts/minute (CPM ...
-
bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
-
bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
-
bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
-
bioRxiv - Biophysics 2021Quote: ... a mixture containing 160 μCi α-32P-adenosine triphosphate (PerkinElmer) and 500 μM ATP was incubated with 100 nM LlCsm complex ...
-
bioRxiv - Cell Biology 2022Quote: ... the plates containing the cells (PerkinElmer, ViewPlate-96, cat# 6005182) were imaged on a CV7000 automated microscope (Yokogawa) ...