Labshake search
Citations for PerkinElmer :
101 - 130 of 130 citations for Siglec 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The plate was incubated in the dark for 10-15 minutes before luminescence was read on a Victor3V 1420 Multilabel Counter (Perkin Elmer).
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... transfection complex was prepared by mixing 50 nM of SMARTPool ON-TARGETplus Human siRNAs (horizon, PerkinElmer) with 3 μl of Lipofectamine RNAiMAX transfection reagent (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Images were capture every minute for continuous 15 mins and analyzed by the Living Image® software (Perkin Elmer, Waltham, MA, USA). Tumor growth was monitored by measuring tumor length (L ...
-
bioRxiv - Cell Biology 2022Quote: ... Nascent proteins are labeled during translation reactions of 15-30 min at 32°C in RRL by incorporation of radioactive 35S-methionine (Perkin Elmer, USA). Nascent TA proteins were released from the ribosome with 1 mM puromycin and then incubated with 5% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: iPSC-derived dopaminergic neurons were re-plated on day 15 of differentiation at a density of 100,000 cells per well into PerkinElmer Phenoplate 96-well plates (PerkinElmer, cat. no 6055300) and kept in maturation media until imaged (day of differentiation 30 ...
-
bioRxiv - Developmental Biology 2024Quote: ... and/ or -C4 reagent for 15 min followed by incubation with an opal dye (Opal 570; FP1487001KT; Perkin Elmer, Opal 620; FP1488001KT, Perkin Elmer, or Opal 690 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mice were injected IP with 10 µL/g of body weight of 15 mg/mL in vivo grade D-Luciferin (luciferin; PerkinElmer, Waltham, MA), anesthetized with 2-3% isoflurane in an induction chamber ...
-
bioRxiv - Cell Biology 2024Quote: ... the plate was subjected to shaking for 15 min followed by an optical density measurement (OD) at 590 nm using 1420 Multilabel Counter (Perkin Elmer, VICTOR3V). The viable cells in arsenite treated samples were reported as the percentage of viable cells in control (untreated ...
-
bioRxiv - Biochemistry 2021Quote: ... Fully-haploid engineered HAP1 (eHAP) human cells (Essletzbichler et al., 2014) were obtained from Horizon (now PerkinElmer), and were cultured in complete IMDM medium (ThermoFisher # 12440-053 ...
-
bioRxiv - Cell Biology 2020Quote: ... Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer, Waltham, Massachusetts, USA).
-
bioRxiv - Cancer Biology 2019Quote: pWPXLD-3xflag-shank2 (5 μg) was incubated with GST-PRMT7 in the presence of S-adenosyl-methionine (SAM; 15 Ci/mmoL; PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
-
bioRxiv - Genomics 2021Quote: All fluorescence imaging on human kidney tissue was performed using the Opera Phenix High-Content Screening System (PerkinElmer). 5mm cryosections were placed into individual wells of 24-well plates and the entire specimen initially imaged for DAPI at 5X ...
-
bioRxiv - Neuroscience 2022Quote: ... astrocytes were incubated with a mixture of 30 μM of GABA and [3H] GABA (40 μCi, specific activity = 25 Ci / mmol, PerkinElmer, 15 min, 37 ° C.). The supernatant is then removed ...
-
bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
-
bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
-
bioRxiv - Genomics 2019Quote: ... FISH experiments were performed using human fosmids containing non-duplicated region directly labeled by nick-translation with Cy3-dUTP (PerkinElmer), Cy5-dUTP (PerkinElmer) ...
-
bioRxiv - Genomics 2021Quote: Two-color FISH experiments were performed using human fosmid (n=2) or BAC (n=2) clones directly labelled by nick-translation with Cy3-dUTP (PerkinElmer) and fluorescein-dUTP (Enzo ...
-
bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
-
bioRxiv - Bioengineering 2023Quote: ... overexpressing the human FGFR1c together with β-klotho was plated into 384-well micro-titer plates (Perkin Elmer, Cat. #6007480) and incubated at 37°C ...
-
bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... tumors (ca 120,000 cells) consisting of H460 human NSCLC transfected with luciferase and the tdTomato gene (H460 Luc+ tdTomato+, Perkin Elmer France) were injected ectopically into the left flank of 90 mice ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
-
bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
-
bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
-
bioRxiv - Cell Biology 2021Quote: ... Images were acquired for 60 min at 1 frame/15-30 s using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective). Images were analyzed using Volocity Suite (PerkinElmer ...
-
bioRxiv - Systems Biology 2023Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
-
bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
-
bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...