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Citations for PerkinElmer :
51 - 100 of 130 citations for Siglec 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Aptamers (15 pmol) and DNA double stranded oligonucleotides were end-labelled with [γ-32P] ATP (Perkin Elmer) and T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Genetics 2020Quote: ... and fluorescence was measured 15-120 minutes after staining using a Victor X5 multiplate reader (Perkin Elmer). Alternately ...
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bioRxiv - Molecular Biology 2023Quote: ... The fluorescence signal was measured after 15 min of incubation with substrate (Ex/Em340/460; Envision, PerkinElmer). Assay Buffer ...
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bioRxiv - Cell Biology 2021Quote: The human glioblastoma U87 MG-Red-FLuc (Bioware Brite, PerkinElmer, Waltham, Massachusetts, USA) was the authorized cell line in all experiments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Multispectral microscopy of the human biopsies was performed using a Vectra 3.0 (PerkinElmer) equipped with 20x and 40x objectives ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with 15 cycles of PCR and using 1:125 dilution of NEXTflex DNA barcodes (Perkin Elmer,NOVA-514104). The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
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bioRxiv - Molecular Biology 2023Quote: ... Generated AFC signal is detected after 15 min at RT using Ex/Em = 360/460 nM (Envision, PerkinElmer). Final assay concentrations ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 15 mg/mL (at 5 µL/g BW) of D-Luciferin (Perkin Elmer) and imaged ...
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bioRxiv - Neuroscience 2024Quote: ... Imaging was performed after 15 min with an Opera High Content Screening System (Perkin Elmer, Waltham, MA, USA). In the whole screening ...
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bioRxiv - Immunology 2020Quote: ... Bound IgG was detected with Eu-N1 Anti-Human IgG (Perkin Elmer, 1244-330). using DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Genomics 2021Quote: Human primary AMSCs and PHH were plated in 96-well CellCarrier plates (Perkinelmer #6005550). AMSCs were differentiated for 14 days and high content imaging was performed at day 0 ...
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bioRxiv - Cell Biology 2021Quote: Human Fibroblasts Cells were seeded on CellCarrier-384 or 96well Ultra microplate (Perkin Elmer) and incubated for at least 24 h in growth media ...
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bioRxiv - Neuroscience 2021Quote: ... confocal micrographs of around 15 representative worms for each genotype were acquired using a spinning-disc confocal microscope (PerkinElmer). The Index of ATG-9 punctum at Zone 2 of AIY for each genotype was consistent across different confocal settings (data not shown) ...
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bioRxiv - Genetics 2019Quote: ... Membranes were washed with PBS-T for 15 minutes and the signal was developed by ECL Plus (Perkin Elmer) or ECL Prime (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... pH 7.0) and incubated for 15 minutes in pre-warmed uptake buffer that contained the radioactive tracer 65 ZnCl2 (PerkinElmer) and non-radioactive ZnCl2 (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
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bioRxiv - Neuroscience 2022Quote: ... in a 15 mg/mL solution and imaged in an IVIS Lumina II In Vivo Imaging System (Perkin Elmer) at 10-20 minutes post-injection ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Cancer Biology 2024Quote: ... Mesothelial clearance was performed by seeding MeT5A-GFP cells (15) at the density of 1.3x 104 cells/well into 384-well CellCarrier plates (PerkinElmer), pre-coated with 10μg/ml collagen type I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... and/ or -C4 reagent for 15 min followed by incubation with an opal dye (Opal 570; FP1487001KT; Perkin Elmer, Opal 620 ...
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bioRxiv - Neuroscience 2020Quote: ... mammalian CHO K1 cells stably overexpressing apo-aequorin and human Gα16 (ES-000-A24, PerkinElmer) were transiently transfected with Ce-npr-4a/pcDNA3.1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably expressing the human CB1 receptor were obtained from PerkinElmer (#ES-110-C) and utilized for western blots (CB1-CHO-C2 ...
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bioRxiv - Immunology 2023Quote: ... for labeling human lymphocytes with or without inhibitors and for the [3H]-Leu (Perkin Elmer) ribosome transit analysis ...
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bioRxiv - Cell Biology 2020Quote: ... and medium was exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37°C on a spinning disc confocal system (Ultraview Vox, PerkinElmer) with a 100 × 1.4 NA oil-immersion objective ...
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bioRxiv - Cancer Biology 2019Quote: ... Luminescence imaging carried out every 2-3 days using an IVIS luminescence imaging system 10 min after mice were injected with 10 μl/g of body weight 15 mg/mL in vivo grade D-Luciferin (PerkinElmer). Mice were sacrificed by CO2 asphyxiation followed by cervical dislocation if they reached AAALAC-defined endpoints ...
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bioRxiv - Cell Biology 2019Quote: ... and media exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37 °C on a spinning disc confocal system (Ultraview Vox; PerkinElmer) with a 100× 1.4 NA oil-immersion objective ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions containing 200 ng of mRNA and 0.1 mM of D-luciferin were carried out in 5 μL aliquots at 37°C for 15 minutes and the activity of in vitro synthesized luciferase was measured by VictorX5 (PerkinElmer) every 30 seconds.
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bioRxiv - Cancer Biology 2019Quote: ... mice were injected intra-peritoneally with XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate (15 mg/ml, 100ul/10g; Perkin Elmer). The mice were imaged with Xenogen IVUS Spectrum (Perkin Elmer) ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... and then re-fixed in formalin for 15 minutes followed by antigen retrieval as per the manufacturer’s instructions (Perkin Elmer). Blocking was performed for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... for lipid peroxidation for 15 min before addition of treatments and imaging in an Operetta high-content imaging system (PerkinElmer) in a temperature and CO2-controlled chamber at the indicated time intervals ...
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bioRxiv - Biophysics 2023Quote: ... Fluorescence was collected after a pinhole of 100 μm diameter size (P100D, Thorlabs) by an avalanche photodetector (SPCM-AQR-15, PerkinElmer) connected to an SPC-150 (Becker & Hickl ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were given 10 μL/g of D-luciferin (15 mg/mL) (Gold Bio) intraperitoneally and imaged using the IVIS Spectrum imager (PerkinElmer). Luciferase signal was quantified 15 min after injection ...
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bioRxiv - Developmental Biology 2023Quote: ... red and green fluorescence at 15 min intervals over 19h on an Opera Phenix™ High-Content Screening System (PerkinElmer) under standard culture conditions.
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bioRxiv - Microbiology 2023Quote: ... Samples were mixed with the DNA absorption solution (for one sample: 50 uL 5 M NaCl, 15 ul magnetic beads (GMG-252-A-100mL – PerkinElmer), 250 uL 7.8 M guanidium chloride ...
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bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Cancer cell growth was monitored in vivo at the indicated times by retro-orbital injection of 100 μl of 15 mg/mL luciferin (Perkin Elmer) dissolved in 1X PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... All biomarkers were measured in triplicate (intra-assay coefficient of variability [CV] < 15% in all cases) using an EnVision microplate reader (PerkinElmer, Finland) and calibrated to the protein concentration in the sample ...
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bioRxiv - Cell Biology 2019Quote: ... injected intraperitoneally with 150 mg/kg of D-Luciferin solution and imaged at 20 minutes post injection (for tumour monitoring) and 15 minutes post injection (for MSCs) using an IVIS Lumina (Perkin Elmer). Exposure times were optimised to ensure sufficient signal was obtained without saturation ...
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Endometrial Gap Junction Expression - Early Indicators of Endometriosis and Integral to InvasivenessbioRxiv - Cell Biology 2021Quote: ... bright field and digital phase contrast images of 10-15 fields per well were captured on an Operetta automated microscope (Perkin Elmer) at 30 min intervals for approximately 2 hours ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were subsequently washed three times with TBST (15 min each) before visualization of Chemiluminescence activity using Western Lightning ECL reagent (Perkin Elmer) in Chemidoc™ MP Imaging System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... washed twice with the same medium and pulse labeled for 15 min with 35S-Cys/Met (300 μCi, Cat. No. NEG072007MC, Perkin Elmer). After pulse ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were incubated with lids in place for 15 min at room temperature and then luminescence readings were taken using a ViewLux ultra HTS Microplate Imager (Perkin Elmer) with a 2 second exposure time per plate ...
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bioRxiv - Cell Biology 2023Quote: ... and an FF01-531/40-25 filter (Semrock) and was finally detected by an SPCM-AQRH-15 avalanche photodiode (Perkin Elmer). The time-correlated single photon counting module to register detected photon events to excitation pulses was SPC830 (Becker & Hickl) ...