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Citations for PerkinElmer :
401 - 450 of 1110 citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were detected by western blot analysis by using an enhanced chemiluminescence system (Western Lightning– ECL, PerkinElmer). Antibodies used were specific for PTEN (Cell Signaling ...
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bioRxiv - Biochemistry 2021Quote: ... 2020 submitted): namely in the presence of EasyTag EXPRESS 35S Protein Labelling Mix containing [35S] methionine (Perkin Elmer) (0.533 MBq ...
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bioRxiv - Neuroscience 2022Quote: ... 25-30 μg of membrane protein was combined with a fixed concentration (0.57-5.18 nM) of 3H-diprenorphine (PerkinElmer) and concentration curves of KUNB106 or positive control (naloxone for mu and delta ...
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bioRxiv - Microbiology 2020Quote: ... Protein localization in fungal cells was carried out by an UltraVIEW VoX Spinning Disk Confocal Microscope (PerkinElmer, USA) and a Leica DMi8 (Leica ...
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bioRxiv - Cell Biology 2021Quote: ... Viral protein expression was detected by imaging with Opera LX and Phenix high content imaging platforms (Perkin Elmer) and the number of infected cells determined using a Columbus Analysis system (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30-40 μg of membrane protein was incubated with varying concentrations of compound and 0.3 nM [3H]-CP55,940 (PerkinElmer) for 90 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts (15 μg protein) were electrophoresed on SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Perkin Elmer). The membranes were incubated overnight at 40C on rocker with primary antibodies in 5% milk dissolved in 1x PBS tween ...
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bioRxiv - Cancer Biology 2021Quote: Protein synthesis assays were performed by measurement of the incorporation of 3H-Leucine (Perkin Elmer, Waltham, MA USA) into protein ...
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bioRxiv - Immunology 2023Quote: ... 10 µl of Protein A AlphaLISA® Acceptor beads (10 µg/ml final concentration; Perkin Elmer cat# AL101C) were added ...
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bioRxiv - Physiology 2023Quote: ... Protein content was estimated using a diode array NIR spectrometer (DA-7200, Perten Instruments (today PerkinElmer, Waltham, USA)).
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bioRxiv - Cancer Biology 2023Quote: ... BioRad) and protein bands were visualized by Western Lightning Plus ECL system (Catalog # ORT2655 and ORT2755, Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein-bound [3H]ryanodine was separated by filtration through polyethyleneimine-treated glass filters (Filtermat B; PerkinElmer, Waltham, MA) using a Micro 96 Cell Harvester (Skatron Instruments ...
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bioRxiv - Biophysics 2023Quote: The ATPase activities of the protein samples were measured with a UV/Vis Spectrometer (Lambda 35, Perkin Elmer) using an ATP (2 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
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bioRxiv - Developmental Biology 2020Quote: ... 1: 5000 followed by incubation with 1:50 to 1: 100 TSA plus Cyanine 3 solution (Perkin Elmer). To detect two genes at a time ...
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bioRxiv - Cancer Biology 2023Quote: Cells were treated with 1 μCi 1-14C (Perkin Elmer, NEC043X050UC) or 6-14C glucose (Perkin Elmer ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 1 μM [1-14C]-oleic acid (PerkinElmer) diluted in high glucose DMEM containing 0.05% BSA for 1h ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was incubated at 37 °C for 20 min before the RFU of fluorescent signals were read on an Envision microplate reader (Perkin Elmer, Waltham, MA) with excitation wavelength set at 320 nm and emission wavelength set at 400 nm ...
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bioRxiv - Physiology 2020Quote: ... Progesterone was extracted with ethyl ether after overnight equilibration (4°C) of the plasma with 1500 dpm of tritiated progesterone (Perkin Elmer, Rodgau, Ermany). Separation of the organic and aqueous phase was conducted similar to the procedure described for the other hormones ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Donor and acceptor emission were measured at 3 min intervals (during a total of 66 min) at 37°C with an EnVision 2014 Multilabel Reader (PerkinElmer, Waltham, MA, USA) using a 340/60 nm excitation filter and 520/8 nm (acceptor ...
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bioRxiv - Microbiology 2020Quote: ... Amastigotes were incubated at 37°C for 72 hours followed by lysis and measurement of luminescence using the ATPlite assay (Perkin Elmer, Waltham, MA).
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bioRxiv - Microbiology 2023Quote: ... was added to bacterial supernatants and the enzymatic activity was monitored for 30 min at 37°C (380 nm) in a VICTOR2 fluorometer/spectrophotometer (Perkin Elmer, Waltham, MA). The enzymatic activity ([PNA generated (nM ...
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bioRxiv - Biochemistry 2024Quote: ... 2B were prepared in ICM (Molsoft LLC., San Diego, CA). Chemical structures (Fig. 5A, C) were generated using ChemDraw (PerkinElmer informatics, Waltham, MA).
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bioRxiv - Biochemistry 2021Quote: ... bound to albumin (1:1 molar ratio) or 0.1 μM [1-14C]oleic acid (2 μCi/dish Perkin Elmer) bound to albumin (1:1 molar ratio ...
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bioRxiv - Neuroscience 2021Quote: ... CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer) were transiently transfected with ckr-1/pcDNA3.1 ...
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bioRxiv - Immunology 2019Quote: ... Acquired images were used to build spectral library of all the target proteins using inForm Cell Analysis software (PerkinElmer).
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bioRxiv - Biochemistry 2021Quote: ... Samples were prepared in a 96-well plate using the manufacturer “HT Protein Express Assay Sample Buffer” (PerkinElmer, USA) supplemented with 36.4 mM (final concentration ...
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bioRxiv - Genomics 2021Quote: ... Percentage of protein content in the grain was measured using a Perten DA 7000 NIR analyzer (Perkin Elmer, Sweden).
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bioRxiv - Microbiology 2021Quote: ... The final Western blotting bands were visualized using an enhanced chemiluminescence protein detection system (PerkinElmer Life Sciences, Boston, MA).
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bioRxiv - Cancer Biology 2020Quote: ... shRNA expression with surrogate red fluorescent protein (RFP) was tracked by fluorescence (IVIS Spectrum In Vivo Imaging Station, PerkinElmer). For chloroquine xenograft experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Media were replaced with fresh media supplemented with Easytag Express S35 Protein Labeling Mix (PerkinElmer, 100-200 µCi/mL) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by a high content screening system (Operetta Enspire, Perkin Elmer, USA). The integral optical density (IOD ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were incubated with primary and secondary HRP-conjugated antibodies in PBST (5% milk and 0.1% Tween-20) to detect the protein of interest by Western Lightning Plus ECL (PerkinElmer) and ImageQuant LAS 4000 imaging system (GE) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then supplemented with 7.4 MBq of [35S] methionine and [35S] cysteine (EXPRE35S35S Protein Labeling Mix, [35S]-, PerkinElmer) and incubated for 1 h to specifically label newly synthesized mitochondrial proteins ...
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bioRxiv - Immunology 2020Quote: ... The cpms of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We next added 0.8 μl of FLAG-GST-transcription factor proteins to 384-well AlphaPlates using a NanoHead (PerkinElmer) and a Janus Workstation (PerkinElmer) ...
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bioRxiv - Microbiology 2019Quote: ... and the protein band reacting with the antiserum was detected using a chemiluminescence detection system (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells then were radiolabeled for 15 minutes with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer) and chased for indicated times in DMEM supplemented with 5 mM unlabeled methionine and cysteine ...
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bioRxiv - Physiology 2022Quote: ... 60-100 µg protein was incubated with 100 μl TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1 ...
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bioRxiv - Neuroscience 2022Quote: GPCR activation assays were performed using CHO-K1 cells stably expressing the luminescent Ca2+ indicator aequorin and the promiscuous Gα16 protein (ES-000-A24 cell line, PerkinElmer). The cells were transfected with a dmsr-7/pcDNA3.1 plasmid using lipofectamine LTX and Plus reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... aliquots of derivatized lysates containing 15 µg of protein were spotted on a Whatman Protran nitrocellulose membrane (Perkin Elmer). Blots were blocked as above ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...
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bioRxiv - Neuroscience 2022Quote: ... siScrambled or siAbdc1/2 Astrocytes were incubated overnight with DMEM without glucose supplemented with 1% FBS containing 1 μCi/mL 14C-Acetate ([1-14C] acetate, 58 mCi/mmol, PerkinElmer) and or α-cyclo or 50 μM C26:0 ...
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bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytes were incubated with a mixture of 30 μM of GABA and [3H] GABA (40 μCi, specific activity = 25 Ci / mmol, PerkinElmer, 15 min, 37 ° C.). The supernatant is then removed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plate was incubated in the dark for 60 min at room temperature (∼22 °C) and subsequently read on an EnVision Multilabel Reader (PerkinElmer Life and Analytical Sciences), with excitation at 340 nm and measurements of emission at 615 and 665 nm ...
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...