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Citations for PerkinElmer :
301 - 350 of 1110 citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cell motility was acquired using temperature (37°C) and CO2 (5%) controlled Operetta CLS High-Content Analysis System (PerkinElmer, HH16000000) (non-confocal ...
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bioRxiv - Immunology 2019Quote: ... 0.1% BSA) and subsequently incubated for 30 min at 25°C with Nickel Chelate acceptor beads (Perkin Elmer, Waltham, MA) and Streptavidin conjugated donor beads (Perkin Elmer) ...
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bioRxiv - Cell Biology 2019Quote: ... and media exchanged for Leibovitz L-15 CO2-independent medium for imaging at 37 °C on a spinning disc confocal system (Ultraview Vox; PerkinElmer) with a 100× 1.4 NA oil-immersion objective ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions containing 200 ng of mRNA and 0.1 mM of D-luciferin were carried out in 5 μL aliquots at 37°C for 15 minutes and the activity of in vitro synthesized luciferase was measured by VictorX5 (PerkinElmer) every 30 seconds.
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence intensity (Ex = 485 nm/Em = 535 nm) was monitored at 37 °C with a microplate reader VictorIII (Perkin Elmer). A calibration curve was created by measuring multiple concentrations (from 0.001 to 5 μM ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were boiled at 95°C for 10 min and subjected directly to LabChip GX II capillary gel electrophoresis (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Live-cell imaging was performed in a humidified environment at 37°C using a spinning-disc confocal microscopy system (UltraVIEW Vox; PerkinElmer) and a charge-coupled device camera (ORCA-R2 ...
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bioRxiv - Cancer Biology 2021Quote: Bioluminescence signals in exosomes and MVs (3 × 107 EVs/µL) carrying PalmReNL were measured at room temperature (25°C) using a VICTOR Nivo Microplate Reader (PerkinElmer). EVs were incubated in the presence or absence of 1% Triton X-100 for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... with the confocal setting at 40X magnification at 37°C and 5% CO2 and analyzed with Columbus Image Analysis System (PerkinElmer). Each well was excited with 425 nm light and emissions detected at 434-515 nm for CFP and 500-550 nm for YFP ...
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bioRxiv - Neuroscience 2022Quote: ... To quantify α1-3;5GABAAR79 sections were incubated for 60 minutes at 4°C in 1nM [3H]flumazenil (Perkin Elmer, NET757001MC), or in 1nM [3H]flumazenil with 10 µM flunitrazepam (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: Embryos and third instar larval larval brains were imaged at 21°C on a Perkin Elmer ERS spinning disk (Volocity software version 6.3, PerkinElmer Inc.) mounted on a Zeiss Axiovert 200M microscope using a 63X/1.4-NA oil immersion objective and an Orca ER CCD camera (Hamamatsu Photonics) ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 25 °C for 10mins followed by addition of 5 μCi of [γ-32P] ATP(Perkin Elmer). Reactions were incubated for additional 30 mins at 37 °C and were stopped using 3X Laemmli buffer ...
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bioRxiv - Bioengineering 2022Quote: ... The volatiles were desorbed again by heating (200°C) in an Automatic Thermal Desorber (ATD-400, Perkin Elmer, Norwalk, CN), cryofocused on a cold trap (-30 °C) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... The plate was sealed with parafilm and placed in a pre-heated (37°C) Opera Phenix microscope with 40x or 60x water-immersion lens (PerkinElmer) with 5% CO2.
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
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bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2023Quote: ... Live imaging was performed with a Nikon TE2000 microscope at 37°C using Volocity software (Nikon Corporation, Tokyo, Japan; PerkinElmer). Images were captured with 488 nm excitation and 516 nm emission at a rate of 20 images per second for 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... Live cell imaging was performed at 37°C in 5% CO2 for 24 hours using the Opera Phenix High Content Screening System (PerkinElmer). Acquired images were analyzed by using the Harmony software (PerkinElmer).
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bioRxiv - Microbiology 2023Quote: ... Plates were read once every 30 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 550 and 591 nm ...
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bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer). For longer-term proliferation assays ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were incubated for 4 h at 37°C and then the supernatant was removed and incubated with scintillation fluid (PerkinElmer) overnight at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer], 3H uridine [0.1 μCi, Perkin Elmer] or 3H thymidine [0.32 μCi ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer] ...
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bioRxiv - Cell Biology 2024Quote: Images of living embryos were acquired at 23°C using a PerkinElmer ERS spinning disk confocal system mounted on a Zeiss Axiovet 200M microscope using Volocity software (PerkinElmer). A 63X ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then pulsed with 2.20 MBq per sample with Express Protein Labeling Mix (Perkin Elmer) and incubated for 2.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and the radiolabeled proteins were detected and quantified using a Cyclone Plus Phosphor Imager system (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... Cultures were labeled with 80 µCi/mL [35S] EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer) for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and protein expression signals were detected using a chemiluminescence detection kit (Perkin Elmer, Waltham, MA, USA) and visualised using a chemiluminescence imaging system (Fusion Solo S ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then labeled with 0.1 mCi/mL EasyTag EXPRESS 35S Protein Labeling Mix (Perkin Elmer) diluted in labeling media ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using Western Lighting Plus ECL detection reagent (Perkin Elmer, Waltham, MA, USA) and imaged with the ChemiDoc TouchTM system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Microbiology 2020Quote: ... Further 160μl of DNS solution was added in each well and incubated at 100°C for 20 min followed by reading at 570nm in multimode plate reader (Perkin Elmer). All the quantitative assays were done in triplicates and the activity was estimated using the standard graph of glucose.
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bioRxiv - Biophysics 2019Quote: ... The plate was then incubated for 20-48 hours at 30°C in a Victor X3 multimode plate reader (Perkin Elmer). Every 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were then rapidly filtered under vacuum through GF/C glass fiber filter-bottom 96-well microplates (Perkin Elmer, MA, USA) pre-treated with 0.1% polyethyleneimine (PEI ...
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bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
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bioRxiv - Cell Biology 2019Quote: ... AMC fluorescence released by cleavage of substrate by proteolytic activity was recorded in a black 96-well plate after 60 min incubation at 37°C using the Victor 3V Multilabel counter (Perkin Elmer). Specific proteasomal activity was determined by subtracting unspecific AMC fluorescence obtained in the presence of proteasome inhibitor MG132 (20 μM) ...
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bioRxiv - Biochemistry 2021Quote: ... Complexes were incubated for 1h at 37°C and luminescence released by the [14C]-RITA-excited beads was measured in standard microplate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
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bioRxiv - Cell Biology 2020Quote: ... and recorded on a 37°C heated stage of Nikon Ti inverted microscope using Ultraview spinning disk confocal system (Perkin Elmer). For the TEM opening ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Developmental Biology 2022Quote: ... For signal detection sections were incubated with the channel specific HRP for 20 min at 40 °C and incubated with the respective fluorophores (Perkin Elmer: Fluorescein ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...