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Citations for PerkinElmer :
401 - 450 of 505 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Images were taken with a PLAN APO 20x/0.75 dry objective using the confocal spinning disk microscope from Perkin Elmer driven by the Volocity 6.3.0 software and equipped with a Yokogama CSU-X1 scan head ...
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bioRxiv - Evolutionary Biology 2023Quote: Libraries were prepared from 33ng of genomic DNA using a custom miniaturized version of the NEXTFLEX Rapid XP kit protocol (PerkinElmer) that was automated on a Sciclone NGSx liquid handler (PerkinElmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Neuroscience 2022Quote: ... suspended cortical cells from each embryo were seeded in poly-L-lysine-coated 24-well plates (View Plate 96, PerkinElmer) at 250000 cells/well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... whereupon 200uL from each well was transferred to transparent 96 well plates for OD600 measures by a Victor3 microtiter plate reader (PerkinElmer). Minimum inhibitory concentrations were interpreted as the lowest concentration of CTX maintained OD600 at less than 0.1 ...
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bioRxiv - Biochemistry 2023Quote: ... The hydrolysis reaction was then measured by absorbance at 630nm (read time of 0.1s per well, Victor 3 from Perkin Elmer) after incubating with malachite green reagent for a minimum of 8min under agitation.
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bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2023Quote: ... specific activity 275 mCi/ mmol) and [3H]mannitol (#NET101001MC, Lot: 3632303, specific activity 12.3 Ci/ mmol) were purchased from Perkin Elmer. [O-methyl-3H]amisulpride (MW 374.8 g/mol ...
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bioRxiv - Immunology 2023Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 50 or 100 µl of a 1:2 dilution of SteadyLite Plus (PerkinElmer) or BioGlo (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... and an aliquot from the supernatant was collected to quantify cAMP using the LANCE cAMP kit (Perkin Elmer, cat#AD0262) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... NIRF images were taken at various time points post-injection under anesthesia and processed by multispectral unmixing to distinguish AuQCs-specific signals from autofluorescence using the Living Image software (PerkinElmer)33 ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was scanned and images were acquired in real-time every 2 hours from 0 to 24 h post-treatment by Opera Phenix High Content Screening System by PerkinElmer, Inc ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3.7 MBq/mL) and [2-14C] acetic acid (2.183 GBq/mmol, 37 MBq/mL) were purchased from PerkinElmer (Waltham, MA). For the ultra[performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ...
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bioRxiv - Neuroscience 2020Quote: ... the pT2 5UAS hSNRNP70-eGFP expression construct was co-injected with a HuC:Gal4 plasmid (gift from Martin Meyer, KCL, UK) together with 100 μM UTP-Cy5 (Perkin Elmer-NEL583001EA) inside a single blastomere at 4-8 cell stage embryos ...
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bioRxiv - Neuroscience 2021Quote: ... Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol; Perkin Elmer) using the MEGAshortscript T7 kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of cells from each culture pulsed with 1 μCi [γ32P]-ATP having a specific activity of 30 Ci/mmol (Perkin Elmer) for 5 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... were based on an initial output from LigPlot+48 and the chemical reaction pathway (Supplementary Fig. 6) was created in ChemDraw (PerkinElmer Informatics).
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bioRxiv - Neuroscience 2021Quote: ... [35S]-GTPγS (1250 Ci/mmol) and [5,8-3H]-adenosine 3,5-cyclic phosphate (34.0 Ci/mmol) were from Perkin Elmer (Boston, MA, USA).
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bioRxiv - Plant Biology 2020Quote: ... 10.5 μg of gDNA was digested with the restriction enzymes EcoRV and XbaI from New England Biolabs (http://www.neb.com) and blotted onto a nylon membrane (GeneScreenPlus; Perkin Elmer, http://www.perkinelmer.com) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Stool samples underwent DNA extraction using chemical and mechanical lysis with magnetic bead-based purification via the Chemagic MSM I with the Chemagic DNA Blood Kit-96 from Perkin Elmer. Prior to extraction on the MSM-I ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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Activation of the Integrated Stress Response overcomes multidrug resistance in FBXW7-deficient cellsbioRxiv - Cancer Biology 2022Quote: ... Cells were subsequently stained with DAPI and images were automatically acquired from each well using an Opera High-Content Screening System (Perkin Elmer) or an ImageXpress Pico Automated Cell Imaging System (Molecular Devices) ...
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bioRxiv - Cell Biology 2022Quote: ... Tumor size of each individual tumor was measured from H&E stained sections using morphometric analysis in Panoramic viewer software (Perkin Elmer). Tumor burden ...
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bioRxiv - Developmental Biology 2022Quote: ... Multicolour fluorescence in situ hybridisation was modified from Hoppler and Vize (Lea et al., 2012) by developing with tyramide amplification (Perkin Elmer) after addition of antisense RNA probes and antibodies conjugated to horseradish peroxidase (Lea et al. ...
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bioRxiv - Microbiology 2020Quote: ... [methyl-14C] CDP-choline (50–60 mCi/mmol) and [diarachidonyl-1-14C] PC (50–60 mCi/mmol) were purchased from Perkin Elmer, Inc (Waltham ...
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bioRxiv - Molecular Biology 2020Quote: Purified protein complexes were incubated at room temperature with a histone substrate at a final concentration of 500nM and approximately 500nM radioactive SAM (Adenosyl-L-methionine S-methyl3H from PerkinElmer (NET155H250UC)) in methyltransferase buffer (10% glycerol ...
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bioRxiv - Neuroscience 2021Quote: ... the fluorescently labeled axon and dendrites were traced from the PV basket cell soma with a Nipkow spinning disk confocal microscope (Volocity, Perkin Elmer, equipped with an Orca camera ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Cancer Biology 2022Quote: ... Photon flux from each well was quantified as photons/second/steradian/cm2 with Living Image Software 4.0 (Perkin Elmer, Shelton, CT). A spectral unmixing algorithm within Living Image ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
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bioRxiv - Biochemistry 2020Quote: ... The 500 nM annealed DNA substrates were then radiolabeled in PNK buffer with PNK enzyme and radioactive ATP [γ32P] from Perkin Elmer at 37°C for 1 hr then diluted in TE buffer (10 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... Images and videos in the manuscript result from maximum projections of z-stacks using Fiji (Schindelin et al., 2012) or 3D reconstructions using Volocity (Perkin Elmer). Surrounding cells were occasionally edited from the field of view using ImageJ or Imaris (Bitplane ...
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bioRxiv - Biochemistry 2020Quote: ... with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol) from Perkin Elmer. Protein Assay (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... the Her2 VHH library was resuspended in 500 µL Triton X-100 based stain buffer (AlphaLISA immunoassay assay buffer from Perkin Elmer; 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... half the medium was removed from all wells and cells were lysed by the addition of 50 µl of BriteLite assay readout solution (Perkin Elmer) to make a a 1:1 dilution into each well ...
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bioRxiv - Cancer Biology 2023Quote: ... the media from each well was transferred to a non-treated 96-well plate and 25ul of AlphaLISA Lysis Buffer (PerkinElmer, #AL001C) was added per well ...
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bioRxiv - Molecular Biology 2023Quote: ... libraries were generated from a mixture of poly(A)+ enriched and rRNA-depleted samples using the NEXTflex® Rapid Directional qRNA-Seq Kit (PerkinElmer). Fragmentation of the RNAs was carried out enzymatically at 95°C for 10 min via the addition of NEXTflex® RNA Fragmentation Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... From each well was transferred 10 µL of the lysates to a 384-well Optiplate™ (Perkin Elmer, catalog no. 6007290). To each well was added Acceptor Mix (5 µL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 100 nL of compound in DMSO from stock plates were added by pin transfer using a Janus Workstation (PerkinElmer, USA). Streptavidin-coated donor beads (15 µg/ml final concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 μL 14C-labelled lipids from the denser organic fraction were combined with 4 mL OptiPhase HiSafe 3 liquid scintillation cocktail (PerkinElmer; 1200.437) and radio-labeling was quantified using a Tri-Carb 2910 TR Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were seeded in 96-well plates under normal growth conditions and fluorescence from tracked live cells was measured using the Operetta High Content Screening System (Perkin Elmer).
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bioRxiv - Bioengineering 2022Quote: ... the major organs were collected from the mice and whole-organ fluorescence was measured via an IVIS Spectrum in vivo imaging system (Perkin Elmer). Images were processed and analyzed by Living Imaging 4.5.5 (Perkin Elmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 100 μL from each culture were transferred to a black 96-well plate to measure the fluorescence (Victor X3, Perkin Elmer).
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bioRxiv - Cell Biology 2022Quote: ... Plates read by laser irradiation of donor beads at 680 nm generating chemiluminescent emission from the acceptor bead at 615 nm using EnVision Multimode Plate Reader (Perkin Elmer).
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bioRxiv - Cell Biology 2022Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Perkin Elmer (Waltham, MA), per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... B cells were lysed in water by osmotic pressure and were transferred from 96 well plates to glass fiber filtermat (8×12 Filtermat A, GF/C, 100/pk, #1450-421, Perkin Elmer). The filters were rinsed with water and sealed in plastic bags with a scintillation cocktail (OptiScint® LLT ...
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bioRxiv - Microbiology 2023Quote: Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer ...
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bioRxiv - Cancer Biology 2023Quote: ... Calcein AM was assessed by fluorescence intensity measurement by well scan from bottom with excitation at 494 nm and emission at 517 nm on Ensight plate reader (Perkinelmer, #HH34000000).