Labshake search
Citations for PerkinElmer :
451 - 500 of 505 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 100 nL of compound in DMSO from stock plates were added by pin transfer using a Janus Workstation (PerkinElmer, USA). Streptavidin-coated donor beads (15 µg/ml final concentration ...
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bioRxiv - Bioengineering 2024Quote: ... Assays were read out with luciferase substrates 2 d after infection by removing the media from the wells and adding 80 uL of a 1:1 dilution of BriteLite in DPBS (BriteLite Plus, Perkin Elmer). Luminescence values were measured using a microplate reader (BioTek Synergy™ HT or Tecan M200) ...
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Synchronous 3D patterning of diverse CNS progenitors generates motor neurons of broad axial identitybioRxiv - Neuroscience 2024Quote: Live imaging of SCOs derived from healthy reporter line BJ- ISL1:mNeonGreen under different patterning approaches was performed with an Operetta CLS (PerkinElmer, USA). Images were taken every 6 hours for multiple days ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenization buffer was prepared as a mix of 10% ‘Lysis & Detection Buffer’ and 90% ‘Stimulation Buffer’ 1× from the IP-One Gq kit (Perkin Elmer). While the exact formula of this buffers is proprietary ...
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bioRxiv - Cancer Biology 2024Quote: ... GFP was assessed by fluorescence intensity measurement by well scan from bottom with excitation at 494 nm and emission at 517 nm on Ensight plate reader (Perkinelmer, #HH34000000).
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bioRxiv - Microbiology 2022Quote: ... we collected 22 mL of gas from each jar and analyzed it using a Clarus 400 gas chromatograph (PerkinElmer Ltd., Beaconsfield, UK) equipped with an automatic split/splitless injector and a flame ionization detector (FID) ...
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bioRxiv - Biochemistry 2022Quote: ... 48 hours post infection 50 µL of medium was removed from all cells were lysed by the addition of 50 µL BriteLite™ assay readout solution (Perkin Elmer) into each well ...
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bioRxiv - Immunology 2021Quote: ... 50μl supernatant was harvested from each well and placed in 96-well Luma Plates containing a dry scintillant (Perkin Elmer, Waltham, MA). Dried plates were counted on a MicroBeta counter (Perkin Elmer ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of luciferase production from glioblastoma cells were measured using D-luciferin (150 μg/ml final concentration, Perkin Elmer, Waltham, MA, USA) and luminescence measurement in a plate reader after 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... The molar concentration of cDNA molecules in the individual libraries was calculated from the double-stranded DNA concentration and the single library average size (determined on a PerkinElmer Labchip GX). An equal number of cDNA molecules from each library were pooled and the final pool was purified once more in order to remove any free primer and prevent index-hopping ...
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bioRxiv - Cell Biology 2022Quote: ... The molar concentration of cDNA molecules in the individual libraries was calculated from the double-stranded DNA concentration and the single library average size (determined on a PerkinElmer Labchip GX). An equal number of cDNA molecules from each library were pooled and the final pool was purified once more in order to remove any free primer and prevent index-hopping ...
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bioRxiv - Bioengineering 2022Quote: ... Average radiance values in p/s/cm2/sr were measured from each well using Living Image software (PerkinElmer, Waltham, Massachusetts, United States).
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Synthetic Biology 2022Quote: ... Medium was removed from all wells and cells were lysed by the addition of 100 μL BriteLite™ assay readout solution (Perkin Elmer) into each well ...
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bioRxiv - Biochemistry 2020Quote: ... Microtiter 384-well Proxiplates for the ThermoFRET stability assay and 384-well Optiplates for association binding experiments were obtained from PerkinElmer (Beaconsfield, UK).
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bioRxiv - Neuroscience 2020Quote: ... 75 µL of supernatant from each well was collected for ATP supernatant detection using the ATPlite Luminescence Assay Kit (Perkin Elmer, 6016943) as described by the kit protocol ...
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bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Immunology 2020Quote: Tumors were isolated from mice and sectioned into 5 micrometer sections for staining with the desired markers (below) using Tyramide Signal Amplification (PerkinElmer, Waltham MA) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Cell Biology 2020Quote: ... The alpha-glucosidase activity was determined by measuring the yellow-colored para-nitrophenol released from pNPG at 405 nm (EnVision Multimode plate reader, Perkin Elmer, USA). The results were expressed as the percentage of the blank.
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bioRxiv - Cancer Biology 2021Quote: ... the PFA-fixed sections were blocked and permeabilized in TNBT (0.1 M Tris, pH 7.4, 150 mM NaCl, 0.5% blocking reagent from Perkin Elmer, 0.5% Triton X-100) for 4h at room temperature ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from 250 mg of leaves ground to powder under liquid nitrogen using the Chemagic DNA Plant Kit (Perkin Elmer, Germany), according to the manufacturer’s instructions on a KingFisher Flex™ (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... frozen pellets were resuspended in 750 μL dH2O from which 200 μL was loaded onto Combusto-Pad (Perkin Elmer, part number 5067034), which were combusted in a sample oxidiser (Model 307 Sample Oxidizer ...
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bioRxiv - Immunology 2024Quote: ... 1:1 mixed with maleate buffer (R2a from the kit)) was added and incubated at room temperature and measured kinetically on an Envision microplate reader (PerkinElmer, Waltham, MA) at 340 nm for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
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An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08) as per the manufacturer’s protocol and sequenced on the Nextseq 500 instrument (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Messenger RNA was isolated from 150 ng of total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer; Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Neuroscience 2023Quote: ... Half of the cell Triton X lysate from each well was pipetted into a scintillation vial and 4 ml of scintillation fluid (#6013329, Ultima GoldTM, Perkin Elmer, USA) was added ...
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bioRxiv - Immunology 2023Quote: ... 50 µL of culture medium was removed from the plates and 30 µL of luciferase substrate (Perkin Elmer Britelite Plus Catalog #6066769) was added ...
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bioRxiv - Microbiology 2024Quote: ... RNA transcripts from the ffh and zapA promoters were labeled with 2– 10 µCi of [α-32P] ATP or [α-32P] UTP (PerkinElmer), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of cell lysate proteins (20-50 μg) or proteins eluted from agarose beads were separated on SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (PerkinElmer Life Sciences). Membranes were blocked 1 h with 3% BSA/TBST and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... 2.5 µl of fixed and stained cells were transferred from 96-well plates into a poly-lysine-coated 384-well Perkin Elmer Cell Carrier Ultra imaging plate (PerkinElmer, Cat. no. 6057500), pre-filled with 25 µl of 1µM SYTOX Green using a Biomek Fx robot ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Timed blood (few drops drawn from the tail vein) and excreta collection was followed by weighing and γ–counting (PerkinElmer Wizard 2480 Automatic Gamma Counter, PerkinElmer, Waltham, MA). Values were corrected for decay and expressed as a percentage of injected dose per gram of tissue (%ID/gram).
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bioRxiv - Biochemistry 2020Quote: The amounts of cAMP in the cytosol were measured by using the time-resolved fluorescence resonance energy transfer (TR-FRET) kit from Perkin Elmer (EnVision Xcite), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... A standard curve consisting of six known standards was made and measured each time using a Victor 3V 1420 Multilabel Counter from Perkin Elmer (Waltham, MA). Fluorescence was measured by using an excitation wavelength of 485 nm and an emission wavelength of 535 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Cell Biology 2021Quote: ... 41.3 Ci mmol−1) and radiocarbon-labelled serotonin binoxalate (14C-serotonin; 54.0 mCi mmol−1) were purchased from Perkin Elmer (Waltham, Massachusetts, USA). Serotonin creatinine sulphate ...
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bioRxiv - Microbiology 2020Quote: ... as previously described.30 Percentage of the infected cells in each well was automatically obtained from 13 fields per well using Harmony software (PerkinElmer, Waltham, MA, USA). Data was normalized to the infected control ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral plasmids encoding for Red-shifted luciferase (RediFect Red-FLuc, RSLucF) or for NucLight Green (NucG) were from Perkin Elmer (Waltham, MA, USA) and Sartorius (Essen Biosciences ...
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bioRxiv - Physiology 2022Quote: ... The assay was a mobility shift assay based on charge differences before and after electrophoretic separation of product from fluorescently labeled substrate read using a Caliper EZ Reader (Perkin Elmer, Boston, MA). All reactions took place in the presence of excess nicotinamide adenine dinucleotide and the known SIRT1 inhibitor suramin was used as a control.
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bioRxiv - Developmental Biology 2022Quote: Intracellular ATP and extracellular lactate levels were measured for each NCC primary cultures after Seahorse analysis using an ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943) and the Lactate Fluorometric Assay kit from BioVision (cat ...
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bioRxiv - Microbiology 2020Quote: ... 20 μCi of radioactive Pi solution (10 μL from a 2 mCi K2H32PO4 at a concentration of 2 mM of K2H32PO4, PerkinElmer cat. no. NEX055) was added to 1 mL of cell suspension ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3’TAMRA fluorescence from 5’UAR was recorded by a single molecule avalanche photodiode (SPAD) (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 600/50 band pass emission filter ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted from oral mucosal tissue using a commercial kit (chemagic™ DNA Buccal Swab Kit, PerkinElmer and DNAdvance Kit, Beckman Coulter). We performed real-time PCR to determine genotypes of DM-associated mutations (SOD1:c.118G
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bioRxiv - Microbiology 2022Quote: ... samples were diluted 1:10 in reagent-grade H2O and iron concentrations were quantified from the diluted samples using a Perkin Elmer Avio 200 mass spectrometer (Perkin Elmer, Waltham, MA) and compared to a standard curve ...
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bioRxiv - Immunology 2023Quote: ... T lymphocyte migration from the upper plate towards chemoattractant solution in the receiver plate was quantified via the ATP detection assay (Perkin Elmer, Waltham, MA). In parallel with the multiscreen assay ...
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bioRxiv - Developmental Biology 2024Quote: Intracellular ATP and glutamine/glutamate levels were measured for each NCC primary cultures after Seahorse analysis using the ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943), and the Glutamine/Glutamate-Go assay (J8021 ...
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bioRxiv - Microbiology 2020Quote: ... signals detected in four spots per well from three independent samples was quantified using the Operetta high content screening system (Perkin Elmer, Waltham, MA, USA) and the built-in Harmony software.
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Cell Biology 2022Quote: ... determined by competitive immunoanalysis using Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) as a detection system (Lance Ultra cAMP kit from Perkin Elmer, Waltham, MA, USA) according to the manufacture instructions ...