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Citations for PerkinElmer :
401 - 450 of 1506 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was then extracted from 2 ug of total RNA per sample using NEXTFLEX® Poly(A) Beads 2.0 (Perkin Elmer, Austin, Texas, USA). Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer ...
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bioRxiv - Microbiology 2024Quote: ... and 4 z positions (0 µm, 0.5 µm, 1 µm, and 1.5 µm) with an Opera Phenix microscope (Perkin Elmer). Colocalization analysis of LC3 ...
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bioRxiv - Microbiology 2023Quote: ... The 14C bicarbonate stock solution (aqueous, activity 5 mCi/5 mL, PerkinElmer, Inc.) was diluted to a final specific activity of 5 µCi/50µL and added to the groundwater sample (43 mL ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Microbiology 2021Quote: ... of which one was [5’-32P] single-end labelled with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol-1) and T4 polynucleotide kinase (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: The ROS production by macrophages was measured by chemiluminescence in the presence of 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) using a thermostatically (37°C) controlled for 1 hr (Envision, PerkinElmer). For nitrite release ...
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bioRxiv - Neuroscience 2020Quote: ... was used as a secondary fluorophore for C1 probes (Gtpbp2, Slc7a1, Chac1) and TSA® Plus Cyanine 3 (PerkinElmer, 1:2,000) was used as a secondary fluorophore for C2 probes (Gtpbp1 ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... were hybridized for 2 h at 40°C followed by signal amplification and developing the HRP channel with TSA Plus fluorophore Cyanine 3 (1:1500) (NEL744001KT, Perkin Elmer) according to the manual ...
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bioRxiv - Cell Biology 2024Quote: ... After two washes of 5min in 1X RNAscope wash buffer slides were incubated with 1:1500 Cyanine 3 amplification reagent (PerkinElmer, #FP1170012UG), or 1:1000 Cyanine 5 amplification reagent (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... and SYBR Gold to the specified dye concentrations (0.03 to 60 μM) and analyzed the sample in a 10 × 2 mm2 cuvette (Perkin Elmer UV/VIS Spectroscopy Cell B0631122) for 5 min for each condition ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Animals were injected intravenously 24h after the last pharmacological treatment with Hypoxisense 680 (2 nmol/100 uL per mouse, Perkin Elmer, Life Sciences, Zaventem, Belgium), and the Hypoxisense 680 was measured in vivo 24h post-injection using the IVIS Spectrum (Perkin Elmer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 4 μL/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 μL/well of ATPLite (PerkinElmer) was dispensed ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 3-D reconstruction was performed with Volocity (PerkinElmer).
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bioRxiv - Immunology 2024Quote: ... mixed with OptiPhase HiSafe 3 scintillation fluid (PerkinElmer) and incubated for 16 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... TR-FRET readout was performed after 1 h incubation at 4°C using an EnVision 2105 Multimode Plate Reader (PerkinElmer) with previously defined measurement parameters53 ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
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bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
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bioRxiv - Microbiology 2021Quote: ... 5-6[3H] (Perkin Elmer) (6 µCi/mL) ...
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bioRxiv - Developmental Biology 2021Quote: ... and cyanine 5 (PerkinElmer #NEL745E001KT). Sections were then incubated in 1X Trueblack Lipofuscin Autofluorescence Quencher (VWR #10119-144 ...
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bioRxiv - Neuroscience 2022Quote: ... D-[5-3H(N)] (PerkinElmer). Cells were incubated for 4 and 6 h after which cell supernatants from duplicate plates were collected at each respective time-point and stored at −20 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-plex positive control probe or with a cocktail of Abcb5-C1 and Abcb4-C2 probes with a 1:750 dilution of TSA-Fluorescein Plus and TSA-Cyanine 3 Plus (PerkinElmer, Shelton, CT), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... image stacks (5 × 1 μm optical sections) were acquired on a spinning disc confocal system (Ultraview Vox, Perkin Elmer) with a 60× 1.4 NA oil-immersion objective (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... of incubation in an isotope solution containing rubidium (86Rb+) (1 μCi/ml, 022-105721-00321-0001, POLATOM) and 3H-mannitol (4 μCi/ml, NET101, Perkin Elmer). 86Rb+ acts as a K+ congener ...
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bioRxiv - Neuroscience 2023Quote: ... Thawed serum samples were diluted with 1xPBS 1:4 and assayed in triplicates using a fluorometer (PerkinElmer, VICTOR3V 1420 Multilabel counter) with excitation/emission of 485 nm / 535 nm (25 nm band-pass filter) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Cancer Biology 2021Quote: In vitro methyltransferase assays were performed using 30 ul reaction buffer (50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2) with 1 ul 3H-AdoMet (Perkin Elmer, specifific activity = 55-85 Ci/mMole,0.55 μCi/ul ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Cancer Biology 2021Quote: 4T1 cells were cultured for 6 h in RPMI medium containing 0.4 μCi ml−1 [5-3H]D-glucose (Perkin Elmer) after which the supernatant was transferred into glass vials sealed with rubber stoppers ...
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bioRxiv - Cancer Biology 2023Quote: ... Each protein (5 µL) was mixed and incubated for 1 h in a 384-well AlphaPlate (PerkinElmer, Waltham, MA, USA). After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi) ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated in TSA plus cyanine 3 (Perkin Elmer) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... The luminescent intensity was measured with VICTOR 3 (PerkinElmer), and the values were normalized to the MAO B protein level measured by Western blotting.
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bioRxiv - Molecular Biology 2024Quote: ... 3 μCi of [α-32P] CTP (PerkinElmer Life Sciences), 1 mM CTP and was carried out at 37°C for 5 min.
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bioRxiv - Developmental Biology 2024Quote: ... The lysate was mixed with OptiPhase HiSafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi ...
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bioRxiv - Microbiology 2021Quote: ... 5 μCi 3H-adenosine (Perkin Elmer), or 10 μM EdA was supplemented ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi [3H]OA (Perkin Elmer) + 0.4 mM OA/0.5% BSA complex ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μCi [γ-32P] ATP (PerkinElmer) and 10 units T4 PNK enzyme (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µCi/ml [3H]-cholesterol (PerkinElmer) was added in the presence of 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... 5 µM [methyl-3H] SAM (PerkinElmer), 10 µM substrate RNA ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 10 min in Cy3 Plus Amplification Reagent diluted 1:300 in 1x Plus Amplification Diluent (TSA Plus Cyanine 3 System, Perkin Elmer, Waltham, MA, NEL744001KT), washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...